LOCUS       Exported                4185 bp DNA     circular SYN 28-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     bsaI(eco31I) site in plasmid GGTCTC
            bsmbI(Esp3I) site in plasmid CGTCTC
FEATURES             Location/Qualifiers
     source          1..4185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..93
                     /label=T5/lacO region
     CDS             115..153
                     /codon_start=1
                     /label=Histag
                     /translation="MRGSHHHHHHTDP"
     CDS             154..174
                     /codon_start=1
                     /label=egfp begin
                     /translation="MVSKGEE"
     CDS             181..849
                     /codon_start=1
                     
                     /label=padron 3to-1
                     /translation="MAVIKPDMKIKLRMEGSVNGHRFRIEGVGLGKPLEGKQSMDLKVK
                     EGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERQMIYEDGGICVAT
                     NDITLDGDCMISEIRFKGVNFPANGPVFQKRTVKWELSHEKLYARDGLLYSDGNYALSL
                     EGGGHYRCDNKTTYKAKKVVQLPDYHWVTHSIVIKSHDKDYSNVNLHEHAEAHSELPRQ
                     A"
     CDS             850..870
                     /codon_start=1
                     /label=egfp end
                     /translation="MDELYK"
     misc_feature    933..1027
                     /label=lambda terminator
     CDS             1070..1729
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      complement(2343..3025)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(3123..3782)
                     /label=AmpR
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatctcacc atcaccatca ccatacggat ccaatggtga gcaagggcga ggagaacaac
      181 atggccgtga ttaaaccaga catgaagatc aagctgcgta tggaaggctc tgtaaatgga
      241 caccgtttca gaattgaagg agttggcctt gggaagcctc ttgagggaaa acagagtatg
      301 gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt gacaatggcg
      361 ttctgttacg gtaacagggt attcgccaaa tacccagaaa atatagtaga ctatttcaag
      421 cagtcgtttc ctgagggcta ctcttgggaa cgacaaatga tttacgaaga cgggggcatt
      481 tgtgtcgcga caaacgacat aaccctggat ggtgactgta tgatctccga aattcgattt
      541 aagggtgtga actttcctgc caatggtcca gttttccaga agaggactgt gaaatgggag
      601 ctatcccacg agaaattgta tgctcgtgat ggactgctgt actctgatgg taactacgct
      661 ctgtcgcttg aaggaggtgg ccattaccga tgtgacaata aaactactta taaagctaag
      721 aaggttgtcc agttgccaga ctatcactgg gtgacccaca gtattgtgat taaaagccac
      781 gacaaagatt acagtaatgt taatctgcat gagcatgccg aagcacattc tgagctgccg
      841 aggcaggcca tggacgagct gtacaagtaa gaattcgaat ctatcaacag gagtccaagc
      901 tcagctaatt aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
      961 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
     1021 gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1081 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     1141 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1201 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1261 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
     1321 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1381 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1441 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1501 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1561 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1621 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1681 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     1741 cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa
     1801 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1861 ctctcctgag taggacaaat ccgccctcta gagctgcctc gcgcgtttcg gtgatgacgg
     1921 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1981 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     2041 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     2101 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     2161 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2221 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2281 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2581 tcggtgtagg tcgttcgctc caaggagggc tgtgtgcacg aaccccccgt tcagcccgac
     2641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2761 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3001 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3421 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3721 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4081 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4141 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcac
//
