LOCUS       Exported               16660 bp DNA     circular SYN 02-AUG-2019
DEFINITION  Clone#4 sequence correctWTC11 iPSCs red after nucleofection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..16660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4428..4461
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          7352..7385
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          11812..12481
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          11812..12481
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="Cloning vector pNEBR-X1Hygro"
     source          11812..12481
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="Cloning vector pNEBR-X1Hygro"
     source          12690..14216
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          12690..14216
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..130)
                     /label=r-PciI-RGBpolyA
                     /note="color: black; sequence: 
                     aattttccttacatgtgcggccgtcctttgagtg"
     polyA_signal    complement(115..569)
                     /label=RBGpolyA (minus last 72bp)
                     /label=RBGpolyA
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     387..409
                     /label=B11-151217-R
                     /note="color: black; sequence: ccaacacactattgcaatgaaaa"
     primer_bind     437..456
                     /label=A1-151002-R
                     /note="A1"
                     /note="color: black; sequence: gatgctcaaggggcttcatg"
     primer_bind     complement(545..555)
                     /label=152028_CG_hISL1_5R
                     /note="color: black; sequence: 
                     GCTTCCATGGTACCGGGGCCAGGGTTCTCTTCCACGTCGCCACATGTCAGCAGGCTGCC
                     T"
     CDS             complement(579..1326)
                     /codon_start=1
                     /label=rtTA3G
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #ffcc00"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(720..732)
                     /label=A37-151006
                     /note="color: black; sequence: GCTGGAAAAGCAGCTGAAGT"
     primer_bind     complement(814..825)
                     /label=B77
                     /note="color: black; sequence: GTGTCTCTCCCCTGCTGAAG"
     primer_bind     950..963
                     /label=A35-151006
                     /note="color: black; sequence: GTTTTCTGAAGCCGTGCTG"
     primer_bind     955..965
                     /label=151028_CG_hLHX3_IT.F
                     /note="color: black; sequence: CTGCTGCTGGCCCTGCTGGC"
     primer_bind     complement(1226..1242)
                     /label=A40-151006
                     /note="color: black; sequence: AGAAAGCTGGCCCAGAAACT"
     primer_bind     complement(1287..1326)
                     /label=DD39
                     /note="161007.2-MSF,Y"
                     /note="color: black; sequence: 
                     atgagccggctggacaagagcaaagtgatcaacagcgccc"
     primer_bind     1305..1324
                     /label=B54-160309
                     /note="specific to NGN2 (corrected),Y"
                     /note="color: black; sequence: tgctcttgtccagccggctc"
     intron          1333..1426
                     /label=Mini-Intron
                     /note="Common feature added by Mo Mandegar
                     "
                     /note="color: #------"
     primer_bind     complement(1351..1369)
                     /label=A2-151002-F
                     /note="A2"
                     /note="color: black; sequence: ctggttattgtgctgtctc"
     primer_bind     complement(1403..1423)
                     /label=AA27-151016-1F
                     /note="A19"
                     /note="color: black; sequence: tctgctaaccatgttcatgcc"
     primer_bind     complement(1408..1428)
                     /label=A41-151006
                     /note="color: black; sequence: gagcctctgctaaccatgttc"
     enhancer        complement(1434..2681)
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
                     /note="This reverse directional feature has 2 segments:
                        1: 1434 .. 2403 / #ffffff / Enhancer Element
                        2: 2404 .. 2681 / #ffffff / Chicken beta-actin Promoter"
     primer_bind     complement(1651..1670)
                     /label=A26-151102-F
                     /note="A26"
                     /note="color: black; sequence: tttgtcccaaatctgtgcgg"
     primer_bind     complement(1658..1678)
                     /label=B04-151217-F
                     /note="color: black; sequence: GGACTTCCTTTGTCCCAAATC"
     primer_bind     complement(2416..2435)
                     /label=B03-151217-F
                     /note="color: black; sequence: GGCCCTATAAAAAGCGAAGC"
     primer_bind     complement(2642..2661)
                     /label=B16-160126-F
                     /note="Y"
                     /note="color: black; sequence: tgcttcactctccccatctc"
     enhancer        2683..3062
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     primer_bind     2830..2848
                     /label=B10-151217-R
                     /note="color: black; sequence: TGCCAAGTGGGCAGTTTAC"
     misc_feature    3063..3216
                     /label=Transcription blocker
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene combined with a synthetic 
                     polyA signal"
                     /note="color: #a6acb3"
     primer_bind     3080..3101
                     /label=f-17-trans.block.
                     /note="color: black; sequence: ttcattacatctgtgtgttggt"
     misc_feature    3217..4427
                     /label=chick cHS4 insulator(1)
                     /note="color: #999999"
     protein_bind    4428..4461
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        4462..4673
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          4674..5612
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     complement(4709..4728)
                     /label=E22
                     /note="JTS,I2,y"
                     /note="color: black; sequence: AAGGGCCATAACCCGTAAAG"
     primer_bind     4752..4771
                     /label=E56
                     /note="Y"
                     /note="color: black; sequence: TGGCTGCAGTACGTGATTCT"
     primer_bind     4908..4925
                     /label=D74
                     /note="MAG,180316,y"
                     /note="color: black; sequence: GCCTGTCTCGCTGCTTTC"
     primer_bind     complement(4911..4930)
                     /label=E1
                     /note="mw,y"
                     /note="color: black; sequence: ttatcgaaagcagcgagaca"
     primer_bind     5309..5328
                     /label=D75
                     /note="MSF,180413.1 and .2,y"
                     /note="color: black; sequence: TGAGTCACCCACACAAAGGA"
     primer_bind     5387..5406
                     /label=D48
                     /note="MSF,180223,y"
                     /note="color: black; sequence: GTCCAGGCACCTCGATTAGT"
     primer_bind     complement(5561..5580)
                     /label=E20
                     /note="JTS,I2,y"
                     /note="color: black; sequence: TCTGAGGCTTGAGAATGAAC"
     primer_bind     5569..5589
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     misc_feature    5629..5634
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             5635..6231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5780..5799
                     /label=B40-160210
                     /note="Y"
                     /note="color: black; sequence: tgcaagaactcttcctcacg"
     primer_bind     complement(5951..5969)
                     /label=B41-160210
                     /note="Y"
                     /note="color: black; sequence: aggccttccatctgttgct"
     primer_bind     6131..6150
                     /label=B31B-160210
                     /note="Y"
                     /note="color: black; sequence: gcaacctccccttctacgag"
     primer_bind     6132..6151
                     /label=B42-160210
                     /note="Y"
                     /note="color: black; sequence: caacctccccttctacgagc"
     primer_bind     6132..6151
                     /label=B18-160210
                     /note="Y"
                     /note="color: black; sequence: caacctccccttctacgagc"
     primer_bind     6238..6257
                     /label=E10
                     /note="mw,y"
                     /note="color: black; sequence: GGAGAGGGCAGAGGAAGTCT"
     CDS             6241..6294
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 6291"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6241..6257)
                     /label=B63-T2A-rev
                     /note="Y"
                     /note="color: black; sequence: agaagacttcctctgccctc"
     primer_bind     complement(6269..6287)
                     /label=E42
                     /note="FWD AAVS1 (BLAST)-Genotyping H52 Insert (T2A) 
                     [Ordered as E83],y"
                     /note="color: black; sequence: gggattctcctccacgtcac"
     primer_bind     complement(6269..6287)
                     /label=E42
                     /note="FWD AAVS1 (BLAST)-Genotyping H52 Insert (T2A) 
                     [Ordered as E83],y"
                     /note="color: black; sequence: gggattctcctccacgtcac"
     misc_feature    6301..6327
                     /label=mycNLS
                     /note="color: #fc6665; direction: RIGHT"
     misc_feature    6343..6369
                     /label=mycNLS
                     /note="color: #fc6665; direction: RIGHT"
     CDS             6397..7107
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     6797..6815
                     /label=B05-151217-F
                     /note="Y"
                     /note="color: black; sequence: GCCCTGTGATGCAGAAGAA"
     primer_bind     6797..6815
                     /label=A75-151217-F
                     /note="Y"
                     /note="color: black; sequence: gccctgtgatgcagaagaa"
     misc_feature    7111..7126
                     /label=PCR2
                     /note="color: #a6acb3"
     polyA_signal    7127..7351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     7137..7156
                     /label=E55
                     /note="Y"
                     /note="color: black; sequence: tagttgccagccatctgttg"
     primer_bind     complement(7253..7273)
                     /label=C06-160304-R
                     /note="Y"
                     /note="color: black; sequence: CCCCCAGAATAGAATGACACC"
     protein_bind    7352..7385
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     enhancer        complement(7397..7627)
                     /label=core insul(1) ApEinfo_revcolor=#690f96
                     /label=core insul(1)
                     /note="ApEinfo_revcolor=#690f96
                     ApEinfo_label=core insul
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff00ff"
                     /note="color: #690f96; direction: LEFT"
     repeat_region   7901..7935
                     /label=3'ITR
                     /note="color: #ff0080"
     misc_feature    complement(7920..7922)
                     /label=3bp spacer
                     /note="ApEinfo_revcolor=#0000a0
                     ApEinfo_fwdcolor=#ff80ff"
                     /note="color: #0000a0; direction: LEFT"
     primer_bind     complement(8454..8473)
                     /label=pLac(F)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
                     /note="color: #19ff25; direction: LEFT"
     primer_bind     8454..8473
                     /label=B19-160210
                     /note="Y"
                     /note="color: black; sequence: tatccgctcacaattccaca"
     primer_bind     complement(8454..8473)
                     /label=A18-MM-SV40PA_1
                     /note="A18"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(8889..8909)
                     /label=E61
                     /note="Y"
                     /note="color: black; sequence: ttatagtcctgtcgggtttcg"
     primer_bind     complement(9453..9472)
                     /label=E60
                     /note="Y"
                     /note="color: black; sequence: cgtgagttttcgttccactg"
     CDS             complement(9590..10447)
                     /label=Amp
                     /note="ApEinfo_revcolor=#c3c2c4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#7ab3ee"
                     /note="color: #c3c2c4; direction: LEFT"
     primer_bind     complement(9976..9996)
                     /label=E59
                     /note="Y"
                     /note="color: black; sequence: acaacatgggggatcatgtaa"
     primer_bind     10292..10311
                     /label=F4
                     /note="basu [Ordered as E92],Y"
                     /note="color: black; sequence: accgctgttgagatccagtt"
     primer_bind     complement(10437..10464)
                     /label=HH28
                     /note="y"
                     /note="color: black; sequence: 
                     attgaaaaaggaagagtatgagtattca"
     primer_bind     11259..11278
                     /label=BII(R)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
                     /note="color: #5381ff; direction: RIGHT"
     repeat_region   join(11321..11333,11365..11366,11368..11383)
                     /label=5'TR
                     /note="This feature has 3 segments:
                        1: 11321 .. 11333 / #00ff00
                        2: 11365 .. 11366 / #00ff00
                        3: 11368 .. 11383 / #00ff00"
     repeat_region   11334..11364
                     /label=5'ITR
                     /note="color: #00ff00"
     misc_feature    11334..11364
                     /label=31bp spacer
                     /note="ApEinfo_revcolor=#ff77e0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff0080"
                     /note="color: #ff0080"
     enhancer        11559..11790
                     /label=core insul ApEinfo_revcolor=green
                     /label=core insul
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#0080ff"
                     /note="color: #0080ff"
     primer_bind     11804..11830
                     /label=CC60
                     /note="color: black; sequence: 
                     ttggaaggatgaggagatTTAATTAAgggaggtggtccctgcagt"
     misc_feature    11804..11811
                     /label=digest with PacI
                     /note="color: #a6acb3"
     misc_feature    11812..16274
                     /label=synthesized insert
                     /note="color: #a6acb3"
     misc_feature    11812..12481
                     /label=Synthesized DNA from GeneWiz
                     /note="color: #a6acb3"
     misc_signal     11812..12481
                     /label=SeqVer (WT AAV R)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_signal     11812..12481
                     /label=SeqVer (SV40 F)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    11812..12481
                     /label=short UCOE
                     /note="blocks chromatin remodelling/silencing
                              p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 
                     12.0px Helvetica; -webkit-text-stroke: #000000}p.p2 
                     {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica; 
                     color: #4787ff; -webkit-text-stroke: #4787ff}span.s1 
                     {font-kerning: none}span.s2 {text-decoration: underline ; 
                     font-kerning: none}It is part of the human HNRPA2B1-CBX3 
                     locus.This is the short version. There is also a longer 
                     fragment, but the short one is supposed to work just as 
                     well.See also these 
                     papers:http://dx.doi.org/10.1182/blood-2006-12-060814http:/
                     /dx.doi.org/10.1038/mt.2010.132   
                     "
                     /note="color: #a6acb3"
     misc_signal     11812..12481
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_signal     11812..12481
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     11823..11842
                     /label=A43-151119
                     /note="Y"
                     /note="color: black; sequence: cctgcagttacgccaatgat"
     primer_bind     complement(12057..12076)
                     /label=A44-151119
                     /note="color: black; sequence: ggggagttgggagcttaaaa"
     primer_bind     12186..12205
                     /label=C54
                     /note="y"
                     /note="color: black; sequence: ctgaggagactccggtcact"
     primer_bind     12287..12306
                     /label=EE38
                     /note="y"
                     /note="color: black; sequence: cgcctccgccctccccctag"
     primer_bind     complement(12456..12475)
                     /label=A45-151119
                     /note="color: black; sequence: gcctacagctcaagccacat"
     misc_feature    complement(12482..12669)
                     /label=SV40 polyA signal
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(12690..14216)
                     /codon_start=1
                     /product="human, from DNASU 
                     http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=74606"
                     /label=SOX9
                     /note="color: #993366"
                     /translation="MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTEN
                     TRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPH
                     VKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRV
                     QHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVH
                     SPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSD
                     VISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQ
                     APPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKT
                     EQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQ
                     GTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP"
     primer_bind     complement(12819..12837)
                     /label=A4
                     /note="color: black; sequence: cggcctctactccaccttc"
     primer_bind     13009..13028
                     /label=A10
                     /note="color: black; sequence: ctcagctgctccgtcttgat"
     primer_bind     13232..13241
                     /label=LL39
                     /note="color: black; sequence: 
                     ttgctcaccattGCGATCGCggtggcgaccggggtggc"
     primer_bind     complement(13343..13362)
                     /label=A3
                     /note="color: black; sequence: catcgagaccttcgatgtca"
     primer_bind     13485..13502
                     /label=A9
                     /note="color: black; sequence: gtcggttttgggggtggt"
     primer_bind     complement(13821..13840)
                     /label=A2
                     /note="color: black; sequence: agtacccgcacttgcacaac"
     primer_bind     14016..14035
                     /label=A8
                     /note="color: black; sequence: gtcctcctcgctctccttct"
     misc_feature    14217..14222
                     /label=Kozak
                     /note="color: #a6acb3"
     misc_feature    14252..14702
                     /label=pTRE3G-BI
                     /note="bi-directional Tre3G promoter (sequence from 
                     Clontech)"
                     /note="color: #a6acb3"
     misc_feature    14739..14744
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             14745..16274
                     /codon_start=1
                     /product="transcript variant 1, homo sapiansmost highly 
                     expressed variant in astrocytes
                     "
                     /label=NFIA.1
                     /note="color: #993366"
                     /translation="MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHE
                     KRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCV
                     LSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCV
                     QPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGV
                     FSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSV
                     EDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTS
                     SLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEF
                     VQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKP
                     PTTSTEGGAASPTSPTYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWYLG"
     primer_bind     complement(14825..14845)
                     /label=A11
                     /note="color: black; sequence: tgcaggttgaaccatgtgtag"
     primer_bind     14994..15012
                     /label=A5
                     /note="color: black; sequence: cgacccgaatatcgagagg"
     primer_bind     complement(15355..15374)
                     /label=A12
                     /note="color: black; sequence: atgtccattttctggctggt"
     primer_bind     15510..15529
                     /label=A6
                     /note="color: black; sequence: agcatgagtccaggagcaat"
     primer_bind     complement(15831..15850)
                     /label=A13
                     /note="color: black; sequence: tgaagagtcgatggtgttgc"
     primer_bind     16019..16037
                     /label=A7
                     /note="color: black; sequence: caaggtgcacaacccattc"
     misc_feature    16275..16278
                     /label=digest with PmeI
                     /note="color: #a6acb3"
     misc_signal     join(16285..16660,1..178)
                     /label=SeqVer (TRE 3G F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     16285..16653
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_signal     16285..16462
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     complement(16289..16306)
                     /label=C38
                     /note="color: black; sequence: caagttggggtgggcgat"
     primer_bind     16292..16311
                     /label=C12
                     /note="n"
                     /note="color: black; sequence: gcccaccccAACTTGtttat"
     polyA_signal    16301..16431
                     /label=SV40polyA
                     /label=SV40 pA
                     /note="Common feature added/edited by Mo MandegarSlightly 
                     longer sequence than the archieved SnapGene Version (131bp 
                     vs 122bp)
                     "
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(16410..16429)
                     /label=C31
                     /note="y"
                     /note="color: black; sequence: gacatgataagatacattga"
     primer_bind     16499..16518
                     /label=B19-160210
                     /note="Y"
                     /note="color: black; sequence: tatccgctcacaattccaca"
     primer_bind     complement(16499..16518)
                     /label=A18-MM-SV40PA_1
                     /note="A18"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
ORIGIN
        1 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
       61 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggacgg ccgcacatgt
      121 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
      181 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
      241 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
      301 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
      361 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
      421 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
      481 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
      541 tgataggcag cctgcacctg aggagtgaat tcacgcgttt tagcctggca gcatatcgag
      601 atcgaagtca tcgagagcgt cagcagggag catatccaga tcaaaatcat ccagggcatc
      661 ggcgggcagc atgtccaggt cgaagtcgtc cagggcgtcg gtgggtccgc cgctctcgca
      721 cttcagctgc ttctcgaggc cgcagatgat cagttccagg ccgaacagga aggcgggctc
      781 ggcgccctgt ctgtcgaaca gctcgatggc ctgcttcagc agggggggca tgctgtcggt
      841 ggtgggtgtc tctctttcct ctttggcgac ctggtgttcc tgttcttcca gcacgcagcc
      901 cagggtgaag tggcccacgg cgctcagggc gtacagggcg ttttccaggc tgaagccctg
      961 ctggcacagg aaggccagct ggttttccag tgtctcgtac tgcttctcgg tgggtctggt
     1021 gcccaggtgc actttggcgc cgtcccggtg gctcagcagg gcgcatctgt agctcttggc
     1081 gttgttccgc aggaagtcct gccagctctc gccttccaga gggcagctgt gggtgtggtg
     1141 ccggtccagc atctcgatgg gcagggcgtc cagcagggcc cgcttgttct tcacgtgcca
     1201 gtacagggtg ggctgttcca cgcccagttt ctgggccagc ttccgggtgg tcaggccctc
     1261 gatgcccacg ccgttcagca gttccagggc gctgttgatc actttgctct tgtccagccg
     1321 gctcatgtcg acattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca
     1381 ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg
     1441 tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc
     1501 gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc
     1561 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg
     1621 gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct
     1681 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc
     1741 cgccggcgct ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc
     1801 ccgccccgcc cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc
     1861 cacgccccgt acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg
     1921 ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc
     1981 acacaccccg cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc
     2041 ccccggccgc gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc
     2101 agcgctcaca gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca
     2161 cgcacgcacc ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc
     2221 tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg
     2281 gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg
     2341 gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgt cgcagcgact
     2401 ccccgcccgc cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa
     2461 aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc
     2521 gccccgcccc tcgccccgcc ccgccccgcc tggcgcgcgc cccccccccc cccccgcccc
     2581 catcgctgca caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg
     2641 ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac
     2701 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     2761 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     2821 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2881 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2941 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     3001 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     3061 tcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     3121 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     3181 tccccagtgc aagtgcaggt gccagaacat ttctctgagc tcacggggac agcccccccc
     3241 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     3301 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     3361 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     3421 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     3481 gaatgacaga atcatagaac ggcctgggtt gcaaaggagc acagtgctca tccagatcca
     3541 accccctgct atgtgcaggg tcatcaacca gcagcccagg ctgcccagag ccacatccag
     3601 cctggccttg aatgcctgca gggatggggc atccacagcc tccttgggca acctgttcag
     3661 tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt
     3721 ctcagtgtaa agccattccc ccttgtccta tcaaggggga gtttgctgtg acattgttgg
     3781 tctggggtga cacatgtttg ccaattcagt gcatcacgga gaggcagatc ttggggataa
     3841 ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt gagggctctg ggacactctc
     3901 caagtcacag cgttcagaac agccttaagg ataagaagat aggatagaag gacaaagagc
     3961 aagttaaaac ccagcatgga gaggagcaca aaaaggccac agacactgct ggtccctgtg
     4021 tctgagcctg catgtttgat ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct
     4081 ggagagatac agctgggtca gtaggactgg gacaggcagc tggagaattg ccatgtagat
     4141 gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct ccaagatccc caagaccaac
     4201 cccaacccac ccaccgtgcc cactggccat gtccctcagt gccacatccc cacagttctt
     4261 catcacctcc agggacggtg acccccccac ctccgtgggc agctgtgcca ctgcagcacc
     4321 gctctttgga gaaggtaaat cttgctaaat ccagcccgac cctcccctgg cacaacgtaa
     4381 ggccattatc tctcatccaa ctccaggacg gagtcagtga gaatattata acttcgtata
     4441 gcatacatta tacgaagtta tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4501 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4561 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4621 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4681 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4741 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     4801 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     4861 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     4921 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     4981 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     5041 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     5101 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     5161 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     5221 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     5281 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     5341 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     5401 attagttctc gcgcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     5461 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     5521 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     5581 cagtggttca aagttttttt cttccatttc aggtgtcgtg atttaaatcc caccatgacc
     5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5821 aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc
     5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6001 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatcggga
     6241 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaccggtc
     6301 cctgccgcta aacgggtcaa actcgacggc ggttctggag gtcctgctgc aaagcgcgtt
     6361 aagttggatg gagggtcagg gggagccacc gccaccatgg tgtctaaggg cgaagagctg
     6421 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     6481 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     6541 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     6601 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     6661 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     6721 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     6781 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     6841 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     6901 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
     6961 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     7021 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     7081 cctagcaaac tggggcacaa gcttaattag gcggccgcta agaattctgt gccttctagt
     7141 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7201 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7261 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7321 aggcatgctg gggatgcggt gggctctatg gataacttcg tatagcatac attatacgaa
     7381 gttatttggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     7441 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     7501 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     7561 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     7621 tgtccctgat tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
     7681 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
     7741 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     7801 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     7861 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     7921 actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg
     7981 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     8041 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     8101 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     8161 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     8221 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     8281 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     8341 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     8401 ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8461 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8521 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8581 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8641 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8701 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8761 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8821 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8881 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8941 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9001 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9061 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9121 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9181 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9241 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9301 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9361 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9421 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9481 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9541 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9601 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9661 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9721 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9781 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9841 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9901 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9961 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10021 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10081 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10141 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10201 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10261 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10321 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10381 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10441 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10501 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10561 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    10621 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    10681 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    10741 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    10801 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    10861 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    10921 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    10981 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
    11041 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11101 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11161 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttttga
    11221 acccgtggag gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat
    11281 gaagatgctc gacacgctgc agaacacgca gctagattaa ccctagaaag ataatcatat
    11341 tgtgacgtac gttaaagata atcatgtgta aaattgacgc atgtgtttta tcggtctgta
    11401 tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt acacttacat
    11461 actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaacaaa
    11521 aactcaaaat ttcttctata aagtaacaaa acttttatga gggacagccc ccccccaaag
    11581 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
    11641 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
    11701 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
    11761 ctgcagacac ctggggggat acggggaaaa ggcctccacg gccttaatta agggaggtgg
    11821 tccctgcagt tacgccaatg ataacccccg ccagaaaaat cttagtagcc ttcccttttt
    11881 gttttccgtg ccccaactcg gcggattgac tcggcccctt ccggaaacac ccgaatcaac
    11941 ttctagtcaa attattgttc acgccgcaat gacccacccc tggcccgcgt ctgtggaact
    12001 gacccctggt gtacaggaga gttcgctgct gaaagtggtc ccaaaggggt actagttttt
    12061 aagctcccaa ctccccctcc cccagcgtct ggaggattcc acaccctcgc accgcagggg
    12121 cgaggaagtg ggcggagtcc ggttttggcg ccagccgctg aggctgccaa gcagaaaagc
    12181 caccgctgag gagactccgg tcactgtcct cgccccgcct cccccttccc tccccttggg
    12241 gaccaccggg cgccacgccg cgaacggtaa gtgccgcggt cgtcggcgcc tccgccctcc
    12301 ccatagggcc ccaattccca gcgggcgcgg cgcgcggccc ctccccccgc cgggcgcgcg
    12361 cccgctgccc cgcccttcgt ggccgcccgg cgtgggcggt gccacccctc cccccggcgg
    12421 ccccgcgcgc agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga
    12481 gcggcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
    12541 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
    12601 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
    12661 tgattatgat ccggatccgg cgcgccttaa ggtcgagtga gctgtgtgta gacgggttgt
    12721 tcccagtgct gggggctgtg ggtctgcggg atggaaggga ccccagaggt gtcggcgatg
    12781 ggggtgtaca tggggcgctg agcggggttc atgtaggtga aggtggagta gaggccggtg
    12841 ccctggcctg ccgcgtggct gtagtaggag ctggagttct ggtggtcggt gtagtcgtac
    12901 tgtgagcggg tgatgggcgg gtaggagggg ctgtagtgtg ggaggttgaa ggggctgtag
    12961 gcgatctgtt ggggcgagtg ctgctgctgc tcgctgtagt ggctggggct cagctgctcc
    13021 gtcttgatgt gcgttcgctg ggactggccc ggctcgctgc tcagcgtggt cagcgtgtgc
    13081 gcctgtggct gctgcggggg tgccgccggc tgctgtgggg gcgccgcctg cggctgcggg
    13141 ggcgcctgcg gggccggcgg ggcctgtggg ggctgctgcg ggggtggcgg cggcgcctgc
    13201 tgcttggaca tccacacgtg gcccgcgctc gccggggtgg ccgcggtgct gctgatgccg
    13261 tagctgcccg tgtaggtgac ctggccgtgc gtggccggca cccccgggtg gccgttgggc
    13321 ggcaggtact ggtcaaactc gttgacatcg aaggtctcga tgttggagat gacgtcgctg
    13381 ctcagctcgc cgatgtccac gtcgcggaag tcgatagggg gctgtctgcc cccctctggc
    13441 aaggggcgcc cctctcgctt caggtcagcc ttgcccggct gcacgtcggt tttgggggtg
    13501 gtgggtgggg tcggtgggcc ctgggattgc cccgagtgct cgccggggga gtgcacctcg
    13561 ctcatgccgg aggaggagtg tggcgagtcg gcctgcagcg ccttgaagat ggcgttgggg
    13621 gagatgtgcg tctgctccgt ggcctcctct gcctccgcct gcccgttctt caccgacttc
    13681 ctccgccgcg gctggtactt gtaatccggg tggtccttct tgtgctgcac gcgcagccgc
    13741 tccgcctcct ccacgaaggg ccgcttctcg ctctcgttca gaagtctcca gagcttgccc
    13801 agcgtcttgc tgagctcggc gttgtgcaag tgcgggtact ggtccgcgag cttcctgcgc
    13861 gccgcctgcg cccacaccat gaaggcgttc atgggccgct tgacgtgcgg cttgttcttg
    13921 ctggagccgt tgacgcgcac cggcatgggc accagcgtcc agtcgtagcc tttgagcacc
    13981 tggctgaccg cctcgcggat gcacacgggg aacttgtcct cctcgctctc cttcttcaga
    14041 tcgggctcgc ccttggggaa cgtgttctcc tggggccgcg tgttctcggt gtccgagccg
    14101 gagcccgacg ggcagggcga gcccgcggag tcctcggaca tggtggggct gggggcgccg
    14161 gacaggccct tctcctgctc gtcggtcatc ttcatgaagg ggtccaggag attcatggtg
    14221 gccctagggg atcccccggg gaattctcca ggcgatctga cggttcacta aacgagctct
    14281 gcttatatag gcctcccacc gtacacgcca cctcgacata ctcgagttta ctccctatca
    14341 gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt
    14401 tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac
    14461 gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca
    14521 gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt ataagcttta
    14581 ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc agatcgcctg
    14641 gagcaattcc acaacacttt tgtcttatac caactttccg taccacttcc taccctcgta
    14701 aagtcgacac cggggcccag atctccgcgg gtttaaacgc caccatgtat tctccgctct
    14761 gtctcaccca ggatgaattt catcctttca tcgaagcact tctgccccac gtccgagcct
    14821 ttgcctacac atggttcaac ctgcaggccc gaaaacgaaa atacttcaaa aaacatgaaa
    14881 agcgtatgtc aaaagaagaa gagagagccg tgaaggatga attgctaagt gaaaaaccag
    14941 aggtcaagca gaagtgggca tctcgacttc tggcaaagtt gcggaaagat atccgacccg
    15001 aatatcgaga ggattttgtt cttacagtta cagggaaaaa acctccatgt tgtgttcttt
    15061 ccaacccaga ccagaaaggc aagatgcgaa gaattgactg cctccgccag gcagataaag
    15121 tctggaggtt ggaccttgtt atggtgattt tgtttaaagg tattccgctg gaaagtactg
    15181 atggcgagcg ccttgtaaag tccccacaat gctctaatcc agggctctgt gtccaacccc
    15241 atcacatagg ggtttctgtt aaggaactcg atttatattt ggcatacttt gtgcatgcag
    15301 cagattcaag tcaatctgaa agtcccagcc agccaagtga cgctgacatt aaggaccagc
    15361 cagaaaatgg acatttgggc ttccaggaca gttttgtcac atcaggtgtt tttagtgtca
    15421 ctgagctagt aagagtgtca cagacaccaa tagctgcagg aactggccca aatttttctc
    15481 tctcagattt ggaaagttct tcatactaca gcatgagtcc aggagcaatg aggaggtctt
    15541 tacccagcac atcctctacg agctccacaa agcgcctcaa gtctgtggag gatgaaatgg
    15601 acagtcctgg tgaggagcca ttttatacag gccaagggcg ctccccagga agtggcagtc
    15661 agtcaagtgg atggcatgaa gtggagccag gaatgccatc tccaaccaca ctgaagaagt
    15721 cggagaagtc tggtttcagc agcccctccc cttcacagac ctcctccctg ggaacggcgt
    15781 tcacacagca tcaccgacct gtcattacag gacccagagc aagtccgcat gcaacaccat
    15841 cgactcttca tttcccgaca tcacccatta tccagcagcc tgggccttac ttctcacacc
    15901 cagccatccg ctatcaccct caggagacgc tgaaagaatt tgtccaactt gtctgccctg
    15961 atgctggtca gcaggctgga caggtggggt tcctcaatcc caatgggagc agccaaggca
    16021 aggtgcacaa cccattcctt cccaccccaa tgttgccacc gccaccgcca ccaccgatgg
    16081 ccaggcctgt gcctctgccg gtgccagaca caaagcctcc aaccacgtca acagaaggag
    16141 gtgcagcctc ccccacgtca ccaacctact cgacacccag cacctccccc gcaaaccgat
    16201 tcgtcagtgt tggaccacgg gatccaagct ttgtaaatat ccctcaacag acacagtcct
    16261 ggtacctggg ataaaaactc gcgaatcgat cgcccacccc aacttgttta ttgcagctta
    16321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    16381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    16441 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    16501 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    16561 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    16621 aaacctgtcg tgccagctgc attaatgaat cggccaacgc
//
