LOCUS       Exported               15250 bp DNA     circular SYN 01-FEB-2022
DEFINITION  Clone#4 sequence correctWTC11 iPSCs red after nucleofection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 1, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..15250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4428..4461
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          7352..7385
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          11812..12481
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="Cloning vector pNEBR-X1Hygro"
     source          11812..12481
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="Cloning vector pNEBR-X1Hygro"
     source          11812..12481
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..130)
                     /label=r-PciI-RGBpolyA
                     /note="color: black; sequence: 
                     aattttccttacatgtgcggccgtcctttgagtg"
     polyA_signal    complement(115..569)
                     /label=RBGpolyA (minus last 72bp)
                     /label=RBGpolyA
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     387..409
                     /label=B11-151217-R
                     /note="color: black; sequence: ccaacacactattgcaatgaaaa"
     primer_bind     437..456
                     /label=A1-151002-R
                     /note="A1"
                     /note="color: black; sequence: gatgctcaaggggcttcatg"
     primer_bind     complement(545..555)
                     /label=152028_CG_hISL1_5R
                     /note="color: black; sequence: 
                     GCTTCCATGGTACCGGGGCCAGGGTTCTCTTCCACGTCGCCACATGTCAGCAGGCTGCC
                     T"
     CDS             complement(579..1326)
                     /codon_start=1
                     /label=rtTA3G
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #ffcc00"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(720..732)
                     /label=A37-151006
                     /note="color: black; sequence: GCTGGAAAAGCAGCTGAAGT"
     primer_bind     complement(814..825)
                     /label=B77
                     /note="color: black; sequence: GTGTCTCTCCCCTGCTGAAG"
     primer_bind     950..963
                     /label=A35-151006
                     /note="color: black; sequence: GTTTTCTGAAGCCGTGCTG"
     primer_bind     955..965
                     /label=151028_CG_hLHX3_IT.F
                     /note="color: black; sequence: CTGCTGCTGGCCCTGCTGGC"
     primer_bind     complement(1226..1242)
                     /label=A40-151006
                     /note="color: black; sequence: AGAAAGCTGGCCCAGAAACT"
     primer_bind     complement(1287..1326)
                     /label=DD39
                     /note="161007.2-MSF,Y"
                     /note="color: black; sequence: 
                     atgagccggctggacaagagcaaagtgatcaacagcgccc"
     primer_bind     1305..1324
                     /label=B54-160309
                     /note="specific to NGN2 (corrected),Y"
                     /note="color: black; sequence: tgctcttgtccagccggctc"
     intron          1333..1426
                     /label=Mini-Intron
                     /note="Common feature added by Mo Mandegar
                     "
                     /note="color: #------"
     primer_bind     complement(1351..1369)
                     /label=A2-151002-F
                     /note="A2"
                     /note="color: black; sequence: ctggttattgtgctgtctc"
     primer_bind     complement(1403..1423)
                     /label=AA27-151016-1F
                     /note="A19"
                     /note="color: black; sequence: tctgctaaccatgttcatgcc"
     primer_bind     complement(1408..1428)
                     /label=A41-151006
                     /note="color: black; sequence: gagcctctgctaaccatgttc"
     enhancer        complement(1434..2681)
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
                     /note="This reverse directional feature has 2 segments:
                        1: 1434 .. 2403 / #ffffff / Enhancer Element
                        2: 2404 .. 2681 / #ffffff / Chicken beta-actin Promoter"
     primer_bind     complement(1651..1670)
                     /label=A26-151102-F
                     /note="A26"
                     /note="color: black; sequence: tttgtcccaaatctgtgcgg"
     primer_bind     complement(1658..1678)
                     /label=B04-151217-F
                     /note="color: black; sequence: GGACTTCCTTTGTCCCAAATC"
     primer_bind     complement(2416..2435)
                     /label=B03-151217-F
                     /note="color: black; sequence: GGCCCTATAAAAAGCGAAGC"
     primer_bind     complement(2642..2661)
                     /label=B16-160126-F
                     /note="Y"
                     /note="color: black; sequence: tgcttcactctccccatctc"
     enhancer        2683..3062
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     primer_bind     2830..2848
                     /label=B10-151217-R
                     /note="color: black; sequence: TGCCAAGTGGGCAGTTTAC"
     misc_feature    3043..3048
                     /label=cut J37 vector with SpeI
                     /note="color: #a6acb3"
     misc_feature    3063..3216
                     /label=Transcription blocker
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene combined with a synthetic 
                     polyA signal"
                     /note="color: #a6acb3"
     primer_bind     3080..3101
                     /label=f-17-trans.block.
                     /note="color: black; sequence: ttcattacatctgtgtgttggt"
     misc_feature    3217..4427
                     /label=chick cHS4 insulator(1)
                     /note="color: #999999"
     protein_bind    4428..4461
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        4462..4673
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          4674..5612
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     complement(4709..4728)
                     /label=E22
                     /note="JTS,I2,y"
                     /note="color: black; sequence: AAGGGCCATAACCCGTAAAG"
     primer_bind     4752..4771
                     /label=E56
                     /note="Y"
                     /note="color: black; sequence: TGGCTGCAGTACGTGATTCT"
     primer_bind     4908..4925
                     /label=D74
                     /note="MAG,180316,y"
                     /note="color: black; sequence: GCCTGTCTCGCTGCTTTC"
     primer_bind     complement(4911..4930)
                     /label=E1
                     /note="mw,y"
                     /note="color: black; sequence: ttatcgaaagcagcgagaca"
     primer_bind     5309..5328
                     /label=D75
                     /note="MSF,180413.1 and .2,y"
                     /note="color: black; sequence: TGAGTCACCCACACAAAGGA"
     primer_bind     5387..5406
                     /label=D48
                     /note="MSF,180223,y"
                     /note="color: black; sequence: GTCCAGGCACCTCGATTAGT"
     primer_bind     complement(5561..5580)
                     /label=E20
                     /note="JTS,I2,y"
                     /note="color: black; sequence: TCTGAGGCTTGAGAATGAAC"
     primer_bind     5569..5589
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     misc_feature    5629..5634
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             5635..6231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5780..5799
                     /label=B40-160210
                     /note="Y"
                     /note="color: black; sequence: tgcaagaactcttcctcacg"
     primer_bind     complement(5951..5969)
                     /label=B41-160210
                     /note="Y"
                     /note="color: black; sequence: aggccttccatctgttgct"
     primer_bind     6131..6150
                     /label=B31B-160210
                     /note="Y"
                     /note="color: black; sequence: gcaacctccccttctacgag"
     primer_bind     6132..6151
                     /label=B42-160210
                     /note="Y"
                     /note="color: black; sequence: caacctccccttctacgagc"
     primer_bind     6132..6151
                     /label=B18-160210
                     /note="Y"
                     /note="color: black; sequence: caacctccccttctacgagc"
     primer_bind     6238..6257
                     /label=E10
                     /note="mw,y"
                     /note="color: black; sequence: GGAGAGGGCAGAGGAAGTCT"
     CDS             6241..6294
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 6291"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6241..6257)
                     /label=B63-T2A-rev
                     /note="Y"
                     /note="color: black; sequence: agaagacttcctctgccctc"
     misc_feature    6301..6327
                     /label=mycNLS
                     /note="color: #fc6665; direction: RIGHT"
     misc_feature    6343..6369
                     /label=mycNLS
                     /note="color: #fc6665; direction: RIGHT"
     CDS             6397..7107
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     6797..6815
                     /label=B05-151217-F
                     /note="Y"
                     /note="color: black; sequence: GCCCTGTGATGCAGAAGAA"
     primer_bind     6797..6815
                     /label=A75-151217-F
                     /note="Y"
                     /note="color: black; sequence: gccctgtgatgcagaagaa"
     misc_feature    7111..7126
                     /label=PCR2
                     /note="color: #a6acb3"
     polyA_signal    7127..7351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     7137..7156
                     /label=E55
                     /note="Y"
                     /note="color: black; sequence: tagttgccagccatctgttg"
     primer_bind     complement(7253..7273)
                     /label=C06-160304-R
                     /note="Y"
                     /note="color: black; sequence: CCCCCAGAATAGAATGACACC"
     protein_bind    7352..7385
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     enhancer        complement(7397..7627)
                     /label=core insul(1) ApEinfo_revcolor=#690f96
                     /label=core insul(1)
                     /note="ApEinfo_revcolor=#690f96
                     ApEinfo_label=core insul
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff00ff"
                     /note="color: #690f96; direction: LEFT"
     repeat_region   7901..7935
                     /label=3'ITR
                     /note="color: #ff0080"
     misc_feature    complement(7920..7922)
                     /label=3bp spacer
                     /note="ApEinfo_revcolor=#0000a0
                     ApEinfo_fwdcolor=#ff80ff"
                     /note="color: #0000a0; direction: LEFT"
     primer_bind     complement(8454..8473)
                     /label=pLac(F)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
                     /note="color: #19ff25; direction: LEFT"
     primer_bind     8454..8473
                     /label=B19-160210
                     /note="Y"
                     /note="color: black; sequence: tatccgctcacaattccaca"
     primer_bind     complement(8454..8473)
                     /label=A18-MM-SV40PA_1
                     /note="A18"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(8889..8909)
                     /label=E61
                     /note="Y"
                     /note="color: black; sequence: ttatagtcctgtcgggtttcg"
     primer_bind     complement(9453..9472)
                     /label=E60
                     /note="Y"
                     /note="color: black; sequence: cgtgagttttcgttccactg"
     CDS             complement(9590..10447)
                     /label=Amp
                     /note="ApEinfo_revcolor=#c3c2c4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#7ab3ee"
                     /note="color: #c3c2c4; direction: LEFT"
     primer_bind     complement(9976..9996)
                     /label=E59
                     /note="Y"
                     /note="color: black; sequence: acaacatgggggatcatgtaa"
     primer_bind     10292..10311
                     /label=F4
                     /note="basu [Ordered as E92],Y"
                     /note="color: black; sequence: accgctgttgagatccagtt"
     primer_bind     complement(10437..10464)
                     /label=HH28
                     /note="y"
                     /note="color: black; sequence: 
                     attgaaaaaggaagagtatgagtattca"
     primer_bind     11259..11278
                     /label=BII(R)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
                     /note="color: #5381ff; direction: RIGHT"
     repeat_region   join(11321..11333,11365..11366,11368..11383)
                     /label=5'TR
                     /note="This feature has 3 segments:
                        1: 11321 .. 11333 / #00ff00
                        2: 11365 .. 11366 / #00ff00
                        3: 11368 .. 11383 / #00ff00"
     misc_feature    11334..11364
                     /label=31bp spacer
                     /note="ApEinfo_revcolor=#ff77e0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff0080"
                     /note="color: #ff0080"
     repeat_region   11334..11364
                     /label=5'ITR
                     /note="color: #00ff00"
     enhancer        11559..11790
                     /label=core insul ApEinfo_revcolor=green
                     /label=core insul
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#0080ff"
                     /note="color: #0080ff"
     primer_bind     11804..11830
                     /label=CC60
                     /note="color: black; sequence: 
                     ttggaaggatgaggagatTTAATTAAgggaggtggtccctgcagt"
     misc_signal     11812..12481
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     11812..12481
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_feature    11812..12481
                     /label=short UCOE
                     /note="blocks chromatin remodelling/silencing
                              p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 
                     12.0px Helvetica; -webkit-text-stroke: #000000}p.p2 
                     {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica; 
                     color: #4787ff; -webkit-text-stroke: #4787ff}span.s1 
                     {font-kerning: none}span.s2 {text-decoration: underline ; 
                     font-kerning: none}It is part of the human HNRPA2B1-CBX3 
                     locus.This is the short version. There is also a longer 
                     fragment, but the short one is supposed to work just as 
                     well.See also these 
                     papers:http://dx.doi.org/10.1182/blood-2006-12-060814http:/
                     /dx.doi.org/10.1038/mt.2010.132   
                     "
                     /note="color: #a6acb3"
     misc_signal     11812..12481
                     /label=SeqVer (SV40 F)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_signal     11812..12481
                     /label=SeqVer (WT AAV R)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    11812..12481
                     /label=Synthesized DNA from GeneWiz
                     /note="color: #a6acb3"
     primer_bind     11823..11842
                     /label=A43-151119
                     /note="Y"
                     /note="color: black; sequence: cctgcagttacgccaatgat"
     misc_feature    12051..12056
                     /label=cut J37 with SpeI
                     /note="color: #a6acb3"
     primer_bind     complement(12057..12076)
                     /label=A44-151119
                     /note="color: black; sequence: ggggagttgggagcttaaaa"
     primer_bind     12186..12205
                     /label=C54
                     /note="y"
                     /note="color: black; sequence: ctgaggagactccggtcact"
     primer_bind     12287..12306
                     /label=EE38
                     /note="y"
                     /note="color: black; sequence: cgcctccgccctccccctag"
     primer_bind     complement(12456..12475)
                     /label=A45-151119
                     /note="color: black; sequence: gcctacagctcaagccacat"
     misc_feature    12482..12487
                     /label=digest with KpnI
                     /note="color: #a6acb3"
     misc_signal     complement(12488..12634)
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: LEFT"
     misc_signal     complement(12488..12634)
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: LEFT"
     misc_signal     complement(12488..12634)
                     /label=SeqVer (TRE 3G F)
                     /note="color: #------; direction: LEFT"
     polyA_signal    complement(12488..12618)
                     /label=SV40polyA
                     /label=SV40 pA
                     /note="Common feature added/edited by Mo MandegarSlightly 
                     longer sequence than the archieved SnapGene Version (131bp 
                     vs 122bp)
                     "
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     12490..12509
                     /label=C31
                     /note="y"
                     /note="color: black; sequence: gacatgataagatacattga"
     primer_bind     complement(12608..12627)
                     /label=C12
                     /note="n"
                     /note="color: black; sequence: gcccaccccAACTTGtttat"
     primer_bind     complement(12608..12627)
                     /label=C12
                     /note="n"
                     /note="color: black; sequence: gcccaccccAACTTGtttat"
     primer_bind     12613..12630
                     /label=C38
                     /note="color: black; sequence: caagttggggtgggcgat"
     CDS             complement(12648..13346)
                     /codon_start=1
                     /label=ascl1
                     /note="color: #993366"
                     /translation="MESSGKMESGAGQQPQPPQPFLPPAACFFATAAAAAAAAAAAAQS
                     AQQQQPQAPPQQAPQLSPVADSQPSGGGHKSAAKQVKRQRSSSPELMRCKRRLNFSGFG
                     YSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIR
                     ALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQ
                     ELLDFTNWFSR"
     misc_feature    13347..13352
                     /label=Kozak
                     /note="color: #a6acb3"
     misc_feature    13388..13838
                     /label=pTRE3G-BI
                     /note="bi-directional Tre3G promoter (sequence from 
                     Clontech)"
                     /note="color: #a6acb3"
     primer_bind     complement(13393..13412)
                     /label=C37
                     /note="color: black; sequence: ccgtaccacttcctaccctc"
     primer_bind     13433..13452
                     /label=A42-151006
                     /note="color: black; sequence: gtgttgtggaattgctccag"
     primer_bind     complement(13458..13478)
                     /label=A13-MM-Tre3GF
                     /note="A13"
                     /note="color: black; sequence: GCTCGTTTAGTGAACCGTCAG"
     primer_bind     complement(13458..13478)
                     /label=A13-MM-Tre3GF
                     /note="A13"
                     /note="color: black; sequence: GCTCGTTTAGTGAACCGTCAG"
     primer_bind     complement(13478..13497)
                     /label=A34-151006
                     /note="color: black; sequence: gggcgcctataaaagcagag"
     primer_bind     13530..13547
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     13566..13583
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     13602..13619
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     13638..13655
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     13674..13696
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     13710..13727
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     13746..13763
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     misc_feature    13856..13861
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             13862..14860
                     /codon_start=1
                     /label=DLX2
                     /note="mouse, same as in Wernig paper"
                     /note="color: #993366"
                     /translation="MTGVFDSLVADMHSTQITASSTYHQHQQPPSGAGAGPGGNSNSSS
                     SNSSLHKPQESPTLPVSTATDSSYYTNQQHPAGGGGGGASPYAHMGSYQYHASGLNNVS
                     YSAKSSYDLGYTAAYTSYAPYGTSSSPVNNEPDKEDLEPEIRIVNGKPKKVRKPRTIYS
                     SFQLAALQRRFQKTQYLALPERAELAASLGLTQTQVKIWFQNRRSKFKKMWKSGEIPTE
                     QHPGASASPPCASPPVSAPASWDFGAPQRMAGGGPGSGGGGAGSSGSSPSSAASAFLGN
                     YPWYHQASGSASHLQATAPLLHPSQTPQAHHHHHHHHHAGGGAPVSAGTIF"
     misc_feature    14861..14868
                     /label=digest with PmeI
                     /note="color: #a6acb3"
     misc_signal     join(14875..15250,1..178)
                     /label=SeqVer (TRE 3G F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     14875..15243
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_signal     14875..15052
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     complement(14879..14896)
                     /label=C38
                     /note="color: black; sequence: caagttggggtgggcgat"
     polyA_signal    14891..15021
                     /label=SV40polyA
                     /label=SV40 pA
                     /note="Common feature added/edited by Mo MandegarSlightly 
                     longer sequence than the archieved SnapGene Version (131bp 
                     vs 122bp)
                     "
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(15000..15019)
                     /label=C31
                     /note="y"
                     /note="color: black; sequence: gacatgataagatacattga"
     primer_bind     15089..15108
                     /label=B19-160210
                     /note="Y"
                     /note="color: black; sequence: tatccgctcacaattccaca"
     primer_bind     complement(15089..15108)
                     /label=A18-MM-SV40PA_1
                     /note="A18"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
ORIGIN
        1 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
       61 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggacgg ccgcacatgt
      121 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
      181 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
      241 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
      301 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
      361 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
      421 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
      481 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
      541 tgataggcag cctgcacctg aggagtgaat tcacgcgttt tagcctggca gcatatcgag
      601 atcgaagtca tcgagagcgt cagcagggag catatccaga tcaaaatcat ccagggcatc
      661 ggcgggcagc atgtccaggt cgaagtcgtc cagggcgtcg gtgggtccgc cgctctcgca
      721 cttcagctgc ttctcgaggc cgcagatgat cagttccagg ccgaacagga aggcgggctc
      781 ggcgccctgt ctgtcgaaca gctcgatggc ctgcttcagc agggggggca tgctgtcggt
      841 ggtgggtgtc tctctttcct ctttggcgac ctggtgttcc tgttcttcca gcacgcagcc
      901 cagggtgaag tggcccacgg cgctcagggc gtacagggcg ttttccaggc tgaagccctg
      961 ctggcacagg aaggccagct ggttttccag tgtctcgtac tgcttctcgg tgggtctggt
     1021 gcccaggtgc actttggcgc cgtcccggtg gctcagcagg gcgcatctgt agctcttggc
     1081 gttgttccgc aggaagtcct gccagctctc gccttccaga gggcagctgt gggtgtggtg
     1141 ccggtccagc atctcgatgg gcagggcgtc cagcagggcc cgcttgttct tcacgtgcca
     1201 gtacagggtg ggctgttcca cgcccagttt ctgggccagc ttccgggtgg tcaggccctc
     1261 gatgcccacg ccgttcagca gttccagggc gctgttgatc actttgctct tgtccagccg
     1321 gctcatgtcg acattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca
     1381 ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg
     1441 tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc
     1501 gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc
     1561 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg
     1621 gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct
     1681 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc
     1741 cgccggcgct ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc
     1801 ccgccccgcc cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc
     1861 cacgccccgt acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg
     1921 ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc
     1981 acacaccccg cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc
     2041 ccccggccgc gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc
     2101 agcgctcaca gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca
     2161 cgcacgcacc ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc
     2221 tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg
     2281 gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg
     2341 gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgt cgcagcgact
     2401 ccccgcccgc cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa
     2461 aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc
     2521 gccccgcccc tcgccccgcc ccgccccgcc tggcgcgcgc cccccccccc cccccgcccc
     2581 catcgctgca caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg
     2641 ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac
     2701 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     2761 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     2821 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2881 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2941 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     3001 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     3061 tcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     3121 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     3181 tccccagtgc aagtgcaggt gccagaacat ttctctgagc tcacggggac agcccccccc
     3241 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     3301 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     3361 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     3421 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     3481 gaatgacaga atcatagaac ggcctgggtt gcaaaggagc acagtgctca tccagatcca
     3541 accccctgct atgtgcaggg tcatcaacca gcagcccagg ctgcccagag ccacatccag
     3601 cctggccttg aatgcctgca gggatggggc atccacagcc tccttgggca acctgttcag
     3661 tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt
     3721 ctcagtgtaa agccattccc ccttgtccta tcaaggggga gtttgctgtg acattgttgg
     3781 tctggggtga cacatgtttg ccaattcagt gcatcacgga gaggcagatc ttggggataa
     3841 ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt gagggctctg ggacactctc
     3901 caagtcacag cgttcagaac agccttaagg ataagaagat aggatagaag gacaaagagc
     3961 aagttaaaac ccagcatgga gaggagcaca aaaaggccac agacactgct ggtccctgtg
     4021 tctgagcctg catgtttgat ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct
     4081 ggagagatac agctgggtca gtaggactgg gacaggcagc tggagaattg ccatgtagat
     4141 gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct ccaagatccc caagaccaac
     4201 cccaacccac ccaccgtgcc cactggccat gtccctcagt gccacatccc cacagttctt
     4261 catcacctcc agggacggtg acccccccac ctccgtgggc agctgtgcca ctgcagcacc
     4321 gctctttgga gaaggtaaat cttgctaaat ccagcccgac cctcccctgg cacaacgtaa
     4381 ggccattatc tctcatccaa ctccaggacg gagtcagtga gaatattata acttcgtata
     4441 gcatacatta tacgaagtta tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4501 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4561 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4621 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4681 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4741 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     4801 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     4861 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     4921 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     4981 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     5041 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     5101 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     5161 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     5221 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     5281 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     5341 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     5401 attagttctc gcgcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     5461 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     5521 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     5581 cagtggttca aagttttttt cttccatttc aggtgtcgtg atttaaatcc caccatgacc
     5641 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5701 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5761 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5821 aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc
     5881 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5941 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6001 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6061 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6121 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6181 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatcggga
     6241 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaccggtc
     6301 cctgccgcta aacgggtcaa actcgacggc ggttctggag gtcctgctgc aaagcgcgtt
     6361 aagttggatg gagggtcagg gggagccacc gccaccatgg tgtctaaggg cgaagagctg
     6421 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     6481 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     6541 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     6601 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     6661 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     6721 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     6781 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     6841 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     6901 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
     6961 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     7021 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     7081 cctagcaaac tggggcacaa gcttaattag gcggccgcta agaattctgt gccttctagt
     7141 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7201 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7261 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     7321 aggcatgctg gggatgcggt gggctctatg gataacttcg tatagcatac attatacgaa
     7381 gttatttggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     7441 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     7501 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     7561 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     7621 tgtccctgat tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
     7681 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
     7741 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     7801 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     7861 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     7921 actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg
     7981 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     8041 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     8101 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     8161 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     8221 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     8281 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     8341 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     8401 ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8461 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8521 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8581 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8641 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8701 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8761 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8821 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8881 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8941 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9001 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9061 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9121 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9181 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9241 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9301 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9361 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9421 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9481 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9541 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9601 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9661 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9721 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9781 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9841 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9901 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9961 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10021 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10081 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10141 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10201 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10261 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10321 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10381 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10441 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10501 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10561 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    10621 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    10681 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    10741 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    10801 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    10861 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    10921 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    10981 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
    11041 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11101 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11161 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttttga
    11221 acccgtggag gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat
    11281 gaagatgctc gacacgctgc agaacacgca gctagattaa ccctagaaag ataatcatat
    11341 tgtgacgtac gttaaagata atcatgtgta aaattgacgc atgtgtttta tcggtctgta
    11401 tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt acacttacat
    11461 actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaacaaa
    11521 aactcaaaat ttcttctata aagtaacaaa acttttatga gggacagccc ccccccaaag
    11581 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
    11641 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
    11701 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
    11761 ctgcagacac ctggggggat acggggaaaa ggcctccacg gccttaatta agggaggtgg
    11821 tccctgcagt tacgccaatg ataacccccg ccagaaaaat cttagtagcc ttcccttttt
    11881 gttttccgtg ccccaactcg gcggattgac tcggcccctt ccggaaacac ccgaatcaac
    11941 ttctagtcaa attattgttc acgccgcaat gacccacccc tggcccgcgt ctgtggaact
    12001 gacccctggt gtacaggaga gttcgctgct gaaagtggtc ccaaaggggt actagttttt
    12061 aagctcccaa ctccccctcc cccagcgtct ggaggattcc acaccctcgc accgcagggg
    12121 cgaggaagtg ggcggagtcc ggttttggcg ccagccgctg aggctgccaa gcagaaaagc
    12181 caccgctgag gagactccgg tcactgtcct cgccccgcct cccccttccc tccccttggg
    12241 gaccaccggg cgccacgccg cgaacggtaa gtgccgcggt cgtcggcgcc tccgccctcc
    12301 ccctagggcc ccaattccca gcgggcgcgg cgcgcggccc ctccccccgc cgggcgcgcg
    12361 cccgctgccc cgcccttcgt ggccgcccgg cgtgggcggt gccacccctc cccccggcgg
    12421 ccccgcgcgc agctcccggc tccctccccc ttcggatgtg gcttgagctg taggcgcgga
    12481 gggtacccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
    12541 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
    12601 agctgcaata aacaagttgg ggtgggcgat cgattcgcga gtttttatct agagaaccag
    12661 ttggtaaagt ccagcagctc ttgttcctct gggctaagag ggtcgtagga gccctcgtcg
    12721 gaggagtagg acgagaccgg agaacccgcc atagagttca agtcgttgga gtagttgggg
    12781 gagatggtgg gcgacaggac gcccgcctga aaggcagcgc tcaccgcgtc gtgctcgtcc
    12841 agcagctgct gcagcgcgcg gatgtactcg accgccgagc gcagcgtctc caccttgctc
    12901 atcttcttgt tggccgcgcc gttggggaca tgctcccgga gggtggcaaa acccaggttg
    12961 accaacttga cccggttgcg ctcgcgctcg ttgcggcgcg ccacggcggc cggctgctgc
    13021 tgtggcaggc tgtagccgaa gccgctgaag ttgagccggc gtttgcagcg catcagttcc
    13081 ggagaggacg agcgctggcg cttgacctgc ttggccgctg acttgtgacc gccccctgag
    13141 ggctggctgt cggccaccgg gctcagctgc ggcgcctgct gcggcggcgc ctgcggctgt
    13201 tgctgctgcg cgctctgagc tgccgcggcc gccgccgctg ccgccgccgc cgcggtcgca
    13261 aagaagcagg ctgcgggagg caggaagggc tgcgggggct gcggctgctg gccggctcca
    13321 ctctccatct tgccagagct ctccatggtg gcgggwcccc cgcggagatc tgggccccgg
    13381 tgtcgacttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg
    13441 gaattgctcc aggcgatctg acggttcact aaacgagctc tgcttttata ggcgcccacc
    13501 gtacacgcct aaagcttata cgttctctat cactgatagg gagtaaactg gatatacgtt
    13561 ctctatcact gatagggagt aaactgtaga tacgttctct atcactgata gggagtaaac
    13621 tggtcatacg ttctctatca ctgataggga gtaaactcct tatacgttct ctatcactga
    13681 tagggagtaa agtctgcata cgttctctat cactgatagg gagtaaactc ttcatacgtt
    13741 ctctatcact gatagggagt aaactcgagt atgtcgaggt ggcgtgtacg gtgggaggcc
    13801 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagaattcg gatccgccac
    13861 catgactgga gtctttgaca gtctggtggc tgatatgcac tcgacccaga tcaccgcctc
    13921 cagcacgtac caccagcacc agcagccccc gagcggtgcg ggcgccggcc ctggcggcaa
    13981 cagcaacagc agcagcagca acagcagcct gcacaagccc caggagtcgc caaccctccc
    14041 ggtgtccacg gctacggaca gcagctacta caccaaccag cagcacccgg cgggcggcgg
    14101 cggcgggggg gcctcgccct acgcgcacat gggctcctac cagtaccacg ccagcggcct
    14161 caacaatgtc tcctactccg ccaaaagcag ctacgacctg ggctacaccg ccgcgtacac
    14221 ctcctacgcg ccctacggca ccagttcgtc tccggtcaac aacgagccgg acaaggaaga
    14281 ccttgagcct gaaatccgaa tagtgaacgg gaagccaaag aaagtccgga aaccacgcac
    14341 catctactcc agtttccagc tggcggccct tcaacgacgc ttccagaaga cccagtatct
    14401 ggccctgcca gagcgagccg agctggcggc gtccctgggc ctcacccaaa ctcaggtcaa
    14461 aatctggttc cagaaccgcc gatccaagtt caagaagatg tggaaaagcg gcgagatacc
    14521 caccgagcag caccctggag ccagcgcttc tcctccttgt gcctccccgc cggtctcggc
    14581 gccagcatcc tgggacttcg gcgcgccgca gcggatggct ggcggcggcc cgggcagcgg
    14641 aggcggcggt gcgggcagct ctggctccag cccgagcagc gccgcctcgg cctttctggg
    14701 aaactacccg tggtatcacc aggcttcggg ctccgcttca cacctgcagg ccacagcgcc
    14761 acttctgcat ccttcgcaga ctccgcaggc gcaccatcac caccatcacc accaccacgc
    14821 aggcgggggc gccccggtga gcgcggggac gattttctaa gtttaaactc gcgaatcgat
    14881 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    14941 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    15001 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    15061 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    15121 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    15181 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    15241 cggccaacgc
//
