LOCUS       Exported               13945 bp DNA     circular SYN 11-NOV-2019
DEFINITION  Clone#4 sequence correctWTC11 iPSCs red after nucleofection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 1, 2022 from SnapGene 6.0.0
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..13945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4428..4461
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          5630..6963
                     /label=Donor sequence
                     /note="color: #fffe11"
     source          5630..6236
                     /label=Donor sequence
                     /note="color: #fffe11"
     source          7205..7238
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          11665..12334
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          11665..12334
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="Cloning vector pNEBR-X1Hygro"
     source          11665..12334
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="Cloning vector pNEBR-X1Hygro"
     primer_bind     complement(97..130)
                     /label=r-PciI-RGBpolyA
                     /note="color: black; sequence: 
                     aattttccttacatgtgcggccgtcctttgagtg"
     polyA_signal    complement(115..569)
                     /label=RBGpolyA (minus last 72bp)
                     /label=RBGpolyA
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     387..409
                     /label=B11-151217-R
                     /note="color: black; sequence: ccaacacactattgcaatgaaaa"
     primer_bind     437..456
                     /label=A1-151002-R
                     /note="A1"
                     /note="color: black; sequence: gatgctcaaggggcttcatg"
     primer_bind     complement(545..555)
                     /label=152028_CG_hISL1_5R
                     /note="color: black; sequence: 
                     GCTTCCATGGTACCGGGGCCAGGGTTCTCTTCCACGTCGCCACATGTCAGCAGGCTGCC
                     T"
     CDS             complement(579..1326)
                     /codon_start=1
                     /label=rtTA3G
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #ffcc00"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(720..732)
                     /label=A37-151006
                     /note="color: black; sequence: GCTGGAAAAGCAGCTGAAGT"
     primer_bind     complement(814..825)
                     /label=B77
                     /note="color: black; sequence: GTGTCTCTCCCCTGCTGAAG"
     primer_bind     950..963
                     /label=A35-151006
                     /note="color: black; sequence: GTTTTCTGAAGCCGTGCTG"
     primer_bind     955..965
                     /label=151028_CG_hLHX3_IT.F
                     /note="color: black; sequence: CTGCTGCTGGCCCTGCTGGC"
     primer_bind     complement(1226..1242)
                     /label=A40-151006
                     /note="color: black; sequence: AGAAAGCTGGCCCAGAAACT"
     primer_bind     complement(1287..1326)
                     /label=DD39
                     /note="161007.2-MSF,Y"
                     /note="color: black; sequence: 
                     atgagccggctggacaagagcaaagtgatcaacagcgccc"
     primer_bind     1305..1324
                     /label=B54-160309
                     /note="specific to NGN2 (corrected),Y"
                     /note="color: black; sequence: tgctcttgtccagccggctc"
     intron          1333..1426
                     /label=Mini-Intron
                     /note="Common feature added by Mo Mandegar
                     "
                     /note="color: #------"
     primer_bind     complement(1351..1369)
                     /label=A2-151002-F
                     /note="A2"
                     /note="color: black; sequence: ctggttattgtgctgtctc"
     primer_bind     complement(1403..1423)
                     /label=AA27-151016-1F
                     /note="A19"
                     /note="color: black; sequence: tctgctaaccatgttcatgcc"
     primer_bind     complement(1408..1428)
                     /label=A41-151006
                     /note="color: black; sequence: gagcctctgctaaccatgttc"
     enhancer        complement(1434..2681)
                     /label=CAG
                     /note="Common feature added by Mo Mandegar"
                     /note="This reverse directional feature has 2 segments:
                        1: 1434 .. 2403 / #ffffff / Enhancer Element
                        2: 2404 .. 2681 / #ffffff / Chicken beta-actin Promoter"
     primer_bind     complement(1651..1670)
                     /label=A26-151102-F
                     /note="A26"
                     /note="color: black; sequence: tttgtcccaaatctgtgcgg"
     primer_bind     complement(1658..1678)
                     /label=B04-151217-F
                     /note="color: black; sequence: GGACTTCCTTTGTCCCAAATC"
     primer_bind     complement(2416..2435)
                     /label=B03-151217-F
                     /note="color: black; sequence: GGCCCTATAAAAAGCGAAGC"
     primer_bind     complement(2642..2661)
                     /label=B16-160126-F
                     /note="Y"
                     /note="color: black; sequence: tgcttcactctccccatctc"
     enhancer        2683..3062
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     primer_bind     2830..2848
                     /label=B10-151217-R
                     /note="color: black; sequence: TGCCAAGTGGGCAGTTTAC"
     misc_feature    3063..3216
                     /label=Transcription blocker
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene combined with a synthetic 
                     polyA signal"
                     /note="color: #a6acb3"
     primer_bind     3080..3101
                     /label=f-17-trans.block.
                     /note="color: black; sequence: ttcattacatctgtgtgttggt"
     misc_feature    3217..4427
                     /label=chick cHS4 insulator(1)
                     /note="color: #999999"
     protein_bind    4428..4461
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     promoter        4462..4673
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          4674..5612
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     complement(4709..4728)
                     /label=E22
                     /note="JTS,I2,y"
                     /note="color: black; sequence: AAGGGCCATAACCCGTAAAG"
     primer_bind     4752..4771
                     /label=E56
                     /note="Y"
                     /note="color: black; sequence: TGGCTGCAGTACGTGATTCT"
     primer_bind     4908..4925
                     /label=D74
                     /note="MAG,180316,y"
                     /note="color: black; sequence: GCCTGTCTCGCTGCTTTC"
     primer_bind     complement(4911..4930)
                     /label=E1
                     /note="mw,y"
                     /note="color: black; sequence: ttatcgaaagcagcgagaca"
     primer_bind     5309..5328
                     /label=D75
                     /note="MSF,180413.1 and .2,y"
                     /note="color: black; sequence: TGAGTCACCCACACAAAGGA"
     primer_bind     5387..5406
                     /label=D48
                     /note="MSF,180223,y"
                     /note="color: black; sequence: GTCCAGGCACCTCGATTAGT"
     primer_bind     complement(5561..5580)
                     /label=E20
                     /note="JTS,I2,y"
                     /note="color: black; sequence: TCTGAGGCTTGAGAATGAAC"
     primer_bind     5569..5589
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     misc_feature    5629..6963
                     /label=synthnesized insert
                     /note="color: #a6acb3"
     misc_signal     5630..6963
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     5630..6963
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     5630..6963
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     5630..6236
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     5630..6236
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     5630..6236
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     5630..5655
                     /label=AS F' KpnI-Bsd
                     /note="color: black; sequence: 
                     gggtaccgccaccatgaagaccttcaacatctcc"
     primer_bind     5630..5650
                     /label=LL62
                     /note="color: black; sequence: 
                     cggcgcctacgctagcgccaccatgaagaccttcaaca"
     CDS             5635..6054
                     /codon_start=1
                     /label=Bsd
                     /note="Common feature added by Mo Mandegar"
                     /note="color: #ff6600"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     misc_signal     5635..6054
                     /label=SeqVer (AAV F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     5956..5976
                     /label=r-77-Blast
                     /note="color: black; sequence: tcagactatgcaccagattgt"
     misc_feature    6064..6129
                     /label=T2A
                     /note="color: #407f02; direction: RIGHT"
     primer_bind     6067..6086
                     /label=E10
                     /note="mw,y"
                     /note="color: black; sequence: GGAGAGGGCAGAGGAAGTCT"
     primer_bind     complement(6070..6086)
                     /label=B63-T2A-rev
                     /note="Y"
                     /note="color: black; sequence: agaagacttcctctgccctc"
     primer_bind     complement(6098..6116)
                     /label=E42
                     /note="FWD AAVS1 (BLAST)-Genotyping H52 Insert (T2A) 
                     [Ordered as E83],y"
                     /note="color: black; sequence: gggattctcctccacgtcac"
     misc_feature    6130..6156
                     /label=mycNLS
                     /note="color: #fc6665; direction: RIGHT"
     misc_feature    6172..6198
                     /label=mycNLS
                     /note="color: #fc6665; direction: RIGHT"
     primer_bind     complement(6219..6251)
                     /label=LL63
                     /note="color: black; sequence: 
                     gtggcgaccggtagcgtggcgaccggggtggcg"
     primer_bind     complement(6219..6251)
                     /label=LL63
                     /note="color: black; sequence: 
                     gtggcgaccggtagcgtggcgaccggggtggcg"
     primer_bind     complement(6219..6251)
                     /label=LL63a
                     /note="color: black; sequence: 
                     gtggcgaccggtagcgtggcgaccggggtggcg"
     primer_bind     complement(6219..6251)
                     /label=LL63a
                     /note="color: black; sequence: 
                     gtggcgaccggtagcgtggcgaccggggtggcg"
     primer_bind     complement(6220..6236)
                     /label=LL39
                     /note="color: black; sequence: 
                     ttgctcaccattGCGATCGCggtggcgaccggggtggc"
     primer_bind     6236..6271
                     /label=DD54
                     /note="color: black; sequence: 
                     tagcgctaccggtcgccaccATGGTGTCCAAGGGCGAAG"
     misc_feature    6247..6252
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             6253..6960
                     /codon_start=1
                     /label=Optimized mApple
                     /note="color: #ff0000"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6669..6688
                     /label=F68
                     /note="Sall1,SH,in cart"
                     /note="color: black; sequence: CGTGATGCAGAAAAAGACCA"
     primer_bind     complement(6890..6910)
                     /label=DD55
                     /note="color: black; sequence: 
                     cgtactgttccacgatggtgTTACTTGTACAGCTCGTCCATTCC"
     primer_bind     6891..6910
                     /label=B27-160210
                     /note="color: black; sequence: CACCATCGTGGAACAGTACG"
     primer_bind     complement(6940..6963)
                     /label=DD55
                     /note="color: black; sequence: 
                     cgtactgttccacgatggtgTTACTTGTACAGCTCGTCCATTCC"
     misc_feature    6964..6979
                     /label=PCR2
                     /note="color: #a6acb3"
     polyA_signal    6980..7204
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     6990..7009
                     /label=E55
                     /note="Y"
                     /note="color: black; sequence: tagttgccagccatctgttg"
     primer_bind     complement(7106..7126)
                     /label=C06-160304-R
                     /note="Y"
                     /note="color: black; sequence: CCCCCAGAATAGAATGACACC"
     protein_bind    7205..7238
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     enhancer        complement(7250..7480)
                     /label=core insul(1) ApEinfo_revcolor=#690f96
                     /label=core insul(1)
                     /note="ApEinfo_revcolor=#690f96
                     ApEinfo_label=core insul
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff00ff"
                     /note="color: #690f96; direction: LEFT"
     repeat_region   7754..7788
                     /label=3'ITR
                     /note="color: #ff0080"
     misc_feature    complement(7773..7775)
                     /label=3bp spacer
                     /note="ApEinfo_revcolor=#0000a0
                     ApEinfo_fwdcolor=#ff80ff"
                     /note="color: #0000a0; direction: LEFT"
     primer_bind     complement(8307..8326)
                     /label=pLac(F)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
                     /note="color: #19ff25; direction: LEFT"
     primer_bind     8307..8326
                     /label=B19-160210
                     /note="Y"
                     /note="color: black; sequence: tatccgctcacaattccaca"
     primer_bind     complement(8307..8326)
                     /label=A18-MM-SV40PA_1
                     /note="A18"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
     primer_bind     complement(8742..8762)
                     /label=E61
                     /note="Y"
                     /note="color: black; sequence: ttatagtcctgtcgggtttcg"
     primer_bind     complement(9306..9325)
                     /label=E60
                     /note="Y"
                     /note="color: black; sequence: cgtgagttttcgttccactg"
     CDS             complement(9443..10300)
                     /label=Amp
                     /note="ApEinfo_revcolor=#c3c2c4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#7ab3ee"
                     /note="color: #c3c2c4; direction: LEFT"
     primer_bind     complement(9829..9849)
                     /label=E59
                     /note="Y"
                     /note="color: black; sequence: acaacatgggggatcatgtaa"
     primer_bind     10145..10164
                     /label=F4
                     /note="basu [Ordered as E92],Y"
                     /note="color: black; sequence: accgctgttgagatccagtt"
     primer_bind     complement(10290..10317)
                     /label=HH28
                     /note="y"
                     /note="color: black; sequence: 
                     attgaaaaaggaagagtatgagtattca"
     primer_bind     11112..11131
                     /label=BII(R)
                     /note="ApEinfo_revcolor=#19ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#5381ff"
                     /note="color: #5381ff; direction: RIGHT"
     repeat_region   join(11174..11186,11218..11219,11221..11236)
                     /label=5'TR
                     /note="This feature has 3 segments:
                        1: 11174 .. 11186 / #00ff00
                        2: 11218 .. 11219 / #00ff00
                        3: 11221 .. 11236 / #00ff00"
     repeat_region   11187..11217
                     /label=5'ITR
                     /note="color: #00ff00"
     misc_feature    11187..11217
                     /label=31bp spacer
                     /note="ApEinfo_revcolor=#ff77e0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff0080"
                     /note="color: #ff0080"
     enhancer        11412..11643
                     /label=core insul ApEinfo_revcolor=green
                     /label=core insul
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#0080ff"
                     /note="color: #0080ff"
     primer_bind     11657..11683
                     /label=CC60
                     /note="color: black; sequence: 
                     ttggaaggatgaggagatTTAATTAAgggaggtggtccctgcagt"
     misc_feature    11665..12334
                     /label=Synthesized DNA from GeneWiz
                     /note="color: #a6acb3"
     misc_signal     11665..12334
                     /label=SeqVer (WT AAV R)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_signal     11665..12334
                     /label=SeqVer (SV40 F)
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    11665..12334
                     /label=short UCOE
                     /note="blocks chromatin remodelling/silencing
                              p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 
                     12.0px Helvetica; -webkit-text-stroke: #000000}p.p2 
                     {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica; 
                     color: #4787ff; -webkit-text-stroke: #4787ff}span.s1 
                     {font-kerning: none}span.s2 {text-decoration: underline ; 
                     font-kerning: none}It is part of the human HNRPA2B1-CBX3 
                     locus.This is the short version. There is also a longer 
                     fragment, but the short one is supposed to work just as 
                     well.See also these 
                     papers:http://dx.doi.org/10.1182/blood-2006-12-060814http:/
                     /dx.doi.org/10.1038/mt.2010.132   
                     "
                     /note="color: #a6acb3"
     misc_signal     11665..12334
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_signal     11665..12334
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     11676..11695
                     /label=A43-151119
                     /note="Y"
                     /note="color: black; sequence: cctgcagttacgccaatgat"
     primer_bind     complement(11910..11929)
                     /label=A44-151119
                     /note="color: black; sequence: ggggagttgggagcttaaaa"
     primer_bind     12039..12058
                     /label=C54
                     /note="y"
                     /note="color: black; sequence: ctgaggagactccggtcact"
     primer_bind     12140..12159
                     /label=EE38
                     /note="y"
                     /note="color: black; sequence: cgcctccgccctccccctag"
     primer_bind     complement(12309..12328)
                     /label=A45-151119
                     /note="color: black; sequence: gcctacagctcaagccacat"
     promoter        12341..12718
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G-sequence identical to 
                     Kyle Denton's AAVS1 integratable non-silencing plasmid
                     "
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    12341..12718
                     /label=added via PCR
                     /note="color: #a6acb3"
     primer_bind     complement(12343..12360)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     complement(12379..12396)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     complement(12410..12432)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     complement(12451..12468)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     complement(12487..12504)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     complement(12523..12540)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     complement(12559..12576)
                     /label=B28-160212-F
                     /note="color: black; sequence: tcactgatagggagtaaagtctg"
     primer_bind     12609..12628
                     /label=A34-151006
                     /note="color: black; sequence: gggcgcctataaaagcagag"
     primer_bind     12628..12648
                     /label=A13-MM-Tre3GF
                     /note="A13"
                     /note="color: black; sequence: GCTCGTTTAGTGAACCGTCAG"
     primer_bind     complement(12654..12673)
                     /label=A42-151006
                     /note="color: black; sequence: gtgttgtggaattgctccag"
     primer_bind     12694..12713
                     /label=C37
                     /note="color: black; sequence: ccgtaccacttcctaccctc"
     primer_bind     12709..12741
                     /label=KK09
                     /note="color: black; sequence: 
                     ccctcgtaaaGGATCCGCCGCCACCATGTTCGT"
     primer_bind     12709..12740
                     /label=BB28-160401
                     /note="color: black; sequence: 
                     ccctcgtaaacttaaggttaattaaGCCGCCACCATGTTCG"
     misc_signal     12725..13555
                     /label=SeqVer (TRE 3G F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     12725..13555
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     12725..13555
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_feature    12725..13555
                     /label=synthesized
                     /note="color: #a6acb3"
     primer_bind     12725..12760
                     /label=151028_XF_hNEUROG2_3F
                     /note="color: black; sequence: 
                     ggttaattaaGCCGCCACCATGTTCGTCAAATCCGAGACCTTGGAG"
     misc_feature    12725..12733
                     /label=Kozac
                     /note="color: #c0c0c0; direction: RIGHT"
     CDS             12734..13552
                     /codon_start=1
                     /label=hNEUROG2
                     /note="color: #339966"
                     /translation="MFVKSETLELKEEEDVLVLLGSASPALAALTPLSSSADEEEEEEP
                     GASGGARRQRGAEAGQGARGGVAAGAEGCRPARLLGLVHDCKRRPSRARAVSRGAKTAE
                     TVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWA
                     LTETLRLADHCGGGGGGLPGALFSEAVLLSPGGASAALSSSGDSPSPASTWSCTNSPAP
                     SSSVSSNSTSPYSCTLSPASPAGSDMDYWQPPPPDKHRYAPHLPIARDCIL"
     primer_bind     12762..12781
                     /label=C28
                     /note="color: black; sequence: TGAAGGAGGAAGAGGACGTG"
     primer_bind     complement(12977..12996)
                     /label=C27
                     /note="color: black; sequence: CGTTTGCAATCGTGTACCAG"
     primer_bind     13166..13183
                     /label=151028_XF_hNEUROG2_IT.F
                     /note="color: blue; sequence: GACGCCAAGCTCACCAAG"
     primer_bind     13170..13189
                     /label=C30
                     /note="color: black; sequence: CCAAGCTCACCAAGATCGAG"
     primer_bind     complement(13286..13307)
                     /label=151028_XF_hNEUROG2_IT.R
                     /note="color: blue; sequence: GCAACACTGCCTCGGAGAAGAG"
     primer_bind     complement(13317..13336)
                     /label=C29
                     /note="color: black; sequence: CAGGGCGGCGCTGGCgCCTC"
     primer_bind     complement(13453..13463)
                     /label=LL63
                     /note="color: black; sequence: 
                     gtggcgaccggtagcgtggcgaccggggtggcg"
     primer_bind     complement(13453..13463)
                     /label=LL63a
                     /note="color: black; sequence: 
                     gtggcgaccggtagcgtggcgaccggggtggcg"
     primer_bind     complement(13454..13463)
                     /label=LL39
                     /note="color: black; sequence: 
                     ttgctcaccattGCGATCGCggtggcgaccggggtggc"
     primer_bind     complement(13528..13552)
                     /label=151028_XF_hNEUROG2_3R
                     /note="color: black; sequence: 
                     GTCGCCACATGTCAGCAGGCTGCCTCTGCCCTCGCCAGAGCCTCTGCTGTGCATCAAGA
                     TACAATCCCTGGCTATGGGG"
     primer_bind     complement(13530..13582)
                     /label=BB29-160401
                     /note="color: black; sequence: 
                     gtgggcgatcgattgcggccgcTTACAAGATACAATCCCTGGCTATGG"
     primer_bind     complement(13533..13581)
                     /label=KK10
                     /note="color: black; sequence: 
                     tgggcgatcgatTCGCGAGTTTAAACTTACAAGATACAATCCCTGGCTA"
     misc_signal     join(13570..13945,1..178)
                     /label=SeqVer (TRE 3G F)
                     /note="color: #------; direction: RIGHT"
     misc_signal     13570..13938
                     /label=SeqVer (WT AAV R)
                     /note="color: #------; direction: RIGHT"
     misc_signal     13570..13747
                     /label=SeqVer (SV40 F)
                     /note="color: #------; direction: RIGHT"
     primer_bind     complement(13574..13591)
                     /label=C38
                     /note="color: black; sequence: caagttggggtgggcgat"
     primer_bind     13577..13596
                     /label=C12
                     /note="n"
                     /note="color: black; sequence: gcccaccccAACTTGtttat"
     polyA_signal    13586..13716
                     /label=SV40polyA
                     /label=SV40 pA
                     /note="Common feature added/edited by Mo MandegarSlightly 
                     longer sequence than the archieved SnapGene Version (131bp 
                     vs 122bp)
                     "
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(13695..13714)
                     /label=C31
                     /note="y"
                     /note="color: black; sequence: gacatgataagatacattga"
     primer_bind     13784..13803
                     /label=B19-160210
                     /note="Y"
                     /note="color: black; sequence: tatccgctcacaattccaca"
     primer_bind     complement(13784..13803)
                     /label=A18-MM-SV40PA_1
                     /note="A18"
                     /note="color: black; sequence: TGTGGAATTGTGAGCGGATA"
ORIGIN
        1 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
       61 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggacgg ccgcacatgt
      121 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
      181 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
      241 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
      301 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
      361 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
      421 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
      481 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
      541 tgataggcag cctgcacctg aggagtgaat tcacgcgttt tagcctggca gcatatcgag
      601 atcgaagtca tcgagagcgt cagcagggag catatccaga tcaaaatcat ccagggcatc
      661 ggcgggcagc atgtccaggt cgaagtcgtc cagggcgtcg gtgggtccgc cgctctcgca
      721 cttcagctgc ttctcgaggc cgcagatgat cagttccagg ccgaacagga aggcgggctc
      781 ggcgccctgt ctgtcgaaca gctcgatggc ctgcttcagc agggggggca tgctgtcggt
      841 ggtgggtgtc tctctttcct ctttggcgac ctggtgttcc tgttcttcca gcacgcagcc
      901 cagggtgaag tggcccacgg cgctcagggc gtacagggcg ttttccaggc tgaagccctg
      961 ctggcacagg aaggccagct ggttttccag tgtctcgtac tgcttctcgg tgggtctggt
     1021 gcccaggtgc actttggcgc cgtcccggtg gctcagcagg gcgcatctgt agctcttggc
     1081 gttgttccgc aggaagtcct gccagctctc gccttccaga gggcagctgt gggtgtggtg
     1141 ccggtccagc atctcgatgg gcagggcgtc cagcagggcc cgcttgttct tcacgtgcca
     1201 gtacagggtg ggctgttcca cgcccagttt ctgggccagc ttccgggtgg tcaggccctc
     1261 gatgcccacg ccgttcagca gttccagggc gctgttgatc actttgctct tgtccagccg
     1321 gctcatgtcg acattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca
     1381 ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg
     1441 tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc
     1501 gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc
     1561 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg
     1621 gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct
     1681 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc
     1741 cgccggcgct ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc
     1801 ccgccccgcc cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc
     1861 cacgccccgt acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg
     1921 ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc
     1981 acacaccccg cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc
     2041 ccccggccgc gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc
     2101 agcgctcaca gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca
     2161 cgcacgcacc ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc
     2221 tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg
     2281 gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg
     2341 gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgt cgcagcgact
     2401 ccccgcccgc cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa
     2461 aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc
     2521 gccccgcccc tcgccccgcc ccgccccgcc tggcgcgcgc cccccccccc cccccgcccc
     2581 catcgctgca caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg
     2641 ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac
     2701 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     2761 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     2821 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2881 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2941 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     3001 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     3061 tcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     3121 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     3181 tccccagtgc aagtgcaggt gccagaacat ttctctgagc tcacggggac agcccccccc
     3241 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     3301 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     3361 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     3421 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     3481 gaatgacaga atcatagaac ggcctgggtt gcaaaggagc acagtgctca tccagatcca
     3541 accccctgct atgtgcaggg tcatcaacca gcagcccagg ctgcccagag ccacatccag
     3601 cctggccttg aatgcctgca gggatggggc atccacagcc tccttgggca acctgttcag
     3661 tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt
     3721 ctcagtgtaa agccattccc ccttgtccta tcaaggggga gtttgctgtg acattgttgg
     3781 tctggggtga cacatgtttg ccaattcagt gcatcacgga gaggcagatc ttggggataa
     3841 ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt gagggctctg ggacactctc
     3901 caagtcacag cgttcagaac agccttaagg ataagaagat aggatagaag gacaaagagc
     3961 aagttaaaac ccagcatgga gaggagcaca aaaaggccac agacactgct ggtccctgtg
     4021 tctgagcctg catgtttgat ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct
     4081 ggagagatac agctgggtca gtaggactgg gacaggcagc tggagaattg ccatgtagat
     4141 gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct ccaagatccc caagaccaac
     4201 cccaacccac ccaccgtgcc cactggccat gtccctcagt gccacatccc cacagttctt
     4261 catcacctcc agggacggtg acccccccac ctccgtgggc agctgtgcca ctgcagcacc
     4321 gctctttgga gaaggtaaat cttgctaaat ccagcccgac cctcccctgg cacaacgtaa
     4381 ggccattatc tctcatccaa ctccaggacg gagtcagtga gaatattata acttcgtata
     4441 gcatacatta tacgaagtta tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4501 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4561 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4621 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4681 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4741 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     4801 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     4861 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     4921 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     4981 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     5041 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     5101 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     5161 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     5221 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     5281 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     5341 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     5401 attagttctc gcgcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     5461 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     5521 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     5581 cagtggttca aagttttttt cttccatttc aggtgtcgtg atttaaatcc caccatgaag
     5641 accttcaaca tctcccagca ggatctagaa ttagtagaag tagcgacaga gaagattaca
     5701 atgctttatg aggataataa acatcatgtg ggagcggcaa ttcgtacgaa aacaggagaa
     5761 atcatttcgg cagtacatat tgaagcgtat ataggacgag taactgtttg tgcagaagcc
     5821 attgcgattg gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat tgtagctgtt
     5881 agacaccctt attctgacga agtagataga agtattcgag tggtaagtcc ttgtggtatg
     5941 tgtagggagt tgatttcaga ctatgcacca gattgttttg tgttaataga aatgaatggc
     6001 aagttagtca aaactacgat tgaagaactc attccactca aatatacccg aaatggatcg
     6061 ggatcgggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     6121 ccaccggtcc ctgccgctaa acgggtcaaa ctcgacggcg gttctggagg tcctgctgca
     6181 aagcgcgtta agttggatgg agggtcaggg ggagccaccg ccaccccggt cgccacgcta
     6241 ccggtcgcca ccatggtgtc caagggcgaa gagaacaaca tggccatcat caaagaattc
     6301 atgcggttca aggtgcacat ggaaggcagc gtgaacggcc acgagttcga gattgaaggc
     6361 gaaggcgagg gcagacccta cgaggctttt cagaccgcca agctgaaagt gaccaaaggc
     6421 ggccctctgc cttttgcctg ggacattctg agccctcagt ttatgtacgg cagcaaggtg
     6481 tacatcaagc accccgccga cattcccgac tacttcaagc tgagcttccc cgagggcttc
     6541 agatgggaga gagtgatgaa cttcgaggac ggcggcatca tccacgtgaa ccaggatagc
     6601 tctctgcagg acggcgtgtt catctacaaa gtgaagctgc ggggcaccaa ctttccctct
     6661 gatggccccg tgatgcagaa aaagaccatg ggctgggaag ccagcgagga aagaatgtac
     6721 cctgaggatg gcgccctgaa gtccgagatc aagaagcggc tgaagctgaa ggatggcggc
     6781 cactatgccg ccgaagtgaa aaccacctac aaggccaaga aacccgtgca gctgcctggc
     6841 gcctacatcg tggatatcaa gctggacatc gtgtcccaca acgaggacta caccatcgtg
     6901 gaacagtacg agagagccga aggcagacac agcacaggcg gaatggacga gctgtacaag
     6961 taagcggccg ctaagaattc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7021 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     7081 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     7141 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     7201 atggataact tcgtatagca tacattatac gaagttattt ggtatggctt tttccccgta
     7261 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     7321 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     7381 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     7441 gtaattacat ccctgggggc tttggggggg ggctgtccct gattctataa caagaaaata
     7501 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     7561 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     7621 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     7681 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     7741 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
     7801 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     7861 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     7921 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     7981 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     8041 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     8101 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     8161 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     8221 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
     8281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9121 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
    10441 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
    10501 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
    10561 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
    10621 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
    10681 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    10741 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    10801 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
    10861 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
    10921 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10981 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    11041 cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc agactcgcgg
    11101 tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc tgcagaacac
    11161 gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgt
    11221 gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat
    11281 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
    11341 atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct ataaagtaac
    11401 aaaactttta tgagggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
    11461 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
    11521 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
    11581 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
    11641 aaaggcctcc acggccttaa ttaagggagg tggtccctgc agttacgcca atgataaccc
    11701 ccgccagaaa aatcttagta gccttccctt tttgttttcc gtgccccaac tcggcggatt
    11761 gactcggccc cttccggaaa cacccgaatc aacttctagt caaattattg ttcacgccgc
    11821 aatgacccac ccctggcccg cgtctgtgga actgacccct ggtgtacagg agagttcgct
    11881 gctgaaagtg gtcccaaagg ggtactagtt tttaagctcc caactccccc tcccccagcg
    11941 tctggaggat tccacaccct cgcaccgcag gggcgaggaa gtgggcggag tccggttttg
    12001 gcgccagccg ctgaggctgc caagcagaaa agccaccgct gaggagactc cggtcactgt
    12061 cctcgccccg cctccccctt ccctcccctt ggggaccacc gggcgccacg ccgcgaacgg
    12121 taagtgccgc ggtcgtcggc gcctccgccc tccccctagg gccccaattc ccagcgggcg
    12181 cggcgcgcgg cccctccccc cgccgggcgc gcgcccgctg ccccgccctt cgtggccgcc
    12241 cggcgtgggc ggtgccaccc ctccccccgg cggccccgcg cgcagctccc ggctccctcc
    12301 cccttcggat gtggcttgag ctgtaggcgc ggagggtacc agtttactcc ctatcagtga
    12361 tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact
    12421 ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat
    12481 gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga
    12541 tagagaacgt atatccagtt tactccctat cagtgataga gaacgtataa gctttaggcg
    12601 tgtacggtgg gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc
    12661 aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaagg
    12721 atccgccgcc accatgttcg tcaaatccga gaccttggag ttgaaggagg aagaggacgt
    12781 gttagtgctg ctcggatcgg cctcccccgc cttggcggcc ctgaccccgc tgtcatccag
    12841 cgccgacgaa gaagaggagg aggagccggg cgcgtcaggc ggggcgcgtc ggcagcgcgg
    12901 ggctgaggcc gggcaggggg cgcggggcgg cgtggctgcg ggtgcggagg gctgccggcc
    12961 cgcacggctg ctgggtctgg tacacgattg caaacggcgc ccttcccggg cgcgggccgt
    13021 ctcccgaggc gccaagacgg ccgagacggt gcagcgcatc aagaagaccc gtagactgaa
    13081 ggccaacaac cgcgagcgaa accgcatgca caacctcaac gcggcactgg acgcgctgcg
    13141 cgaggtgctc cccacgttcc ccgaggacgc caagctcacc aagatcgaga ccctgcgctt
    13201 cgcccacaac tacatctggg cactcaccga gaccctgcgc ctggcggatc actgcggggg
    13261 cggcggcggg ggcctgccgg gggcgctctt ctccgaggca gtgttgctga gcccgggagg
    13321 cgccagcgcc gccctgagca gcagcggaga cagcccctcg cccgcctcca cgtggagttg
    13381 caccaacagc cccgcgccgt cctcctccgt gtcctccaat tccacctccc cctacagctg
    13441 cactttatcg cccgccagcc cggccgggtc agacatggac tattggcagc ccccacctcc
    13501 cgacaagcac cgctatgcac ctcacctccc catagccagg gattgtatct tgtaagttta
    13561 aactcgcgaa tcgatcgccc accccaactt gtttattgca gcttataatg gttacaaata
    13621 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13681 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
    13741 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    13801 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
    13861 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    13921 gctgcattaa tgaatcggcc aacgc
//
