LOCUS       Exported                7806 bp DNA     circular SYN 25-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7806
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        51..627
                     /label=CMV_promoter
                     /label=CMV_immearly_promoter
                     /note="color: #ffffff"
     gene            729..2144
                     /label=FOXA1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2151..2205
                     /label=T2A
                     /note="color: #a6acb3"
     gene            2211..3155
                     /label=miRFP670
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      3179..3449
                     /label=TK_PA_terminator
                     /note="color: #ffffff"
     misc_feature    3503..3809
                     /label=f1_origin
                     /note="color: #a6acb3"
     promoter        3941..4209
                     /label=SV40_promoter
                     /note="color: #ffffff"
     misc_feature    4108..4185
                     /label=SV40_origin
                     /note="color: #a6acb3"
     gene            4327..5115
                     /label=NeoR/KanR
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      5297..5416
                     /label=SV40_PA_terminator
                     /note="color: #ffffff"
     misc_feature    complement(5851..6470)
                     /label=pBR322_origin
                     /note="color: #a6acb3; direction: LEFT"
     gene            complement(6625..7485)
                     /label=Ampicillin
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(7527..7555)
                     /label=AmpR_promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagaat gttaggaact gtgaagatgg aagggcatga aaccagcgac tggaacagct
      781 actacgcaga cacgcaggag gcctactcct ccgtcccggt cagcaacatg aactcaggcc
      841 tgggctccat gaactccatg aacacctaca tgaccatgaa caccatgact acgagcggca
      901 acatgacccc ggcgtccttc aacatgtcct atgccaaccc gggcctaggg gccggcctga
      961 gtcccggcgc agtagccggc atgccggggg gctcggcggg cgccatgaac agcatgactg
     1021 cggccggcgt gacggccatg ggtacggcgc tgagcccgag cggcatgggc gccatgggtg
     1081 cgcagcaggc ggcctccatg aatggcctgg gcccctacgc ggccgccatg aacccgtgca
     1141 tgagccccat ggcgtacgcg ccgtccaacc tgggccgcag ccgcgcgggc ggcggcggcg
     1201 acgccaagac gttcaagcgc agctacccgc acgccaagcc gccctactcg tacatctcgc
     1261 tcatcaccat ggccatccag caggcgccca gcaagatgct cacgctgagc gagatctacc
     1321 agtggatcat ggacctcttc ccctattacc ggcagaacca gcagcgctgg cagaactcca
     1381 tccgccactc gctgtccttc aatgactgct tcgtcaaggt ggcacgctcc ccggacaagc
     1441 cgggcaaggg ctcctactgg acgctgcacc cggactccgg caacatgttc gagaacggct
     1501 gctacttgcg ccgccagaag cgcttcaagt gcgagaagca gccgggggcc ggcggcgggg
     1561 gcgggagcgg aagcgggggc agcggcgcca agggcggccc tgagagccgc aaggacccct
     1621 ctggcgcctc taaccccagc gccgactcgc ccctccatcg gggtgtgcac gggaagaccg
     1681 gccagctaga gggcgcgccg gcccccgggc ccgccgccag cccccagact ctggaccaca
     1741 gtggggcgac ggcgacaggg ggcgcctcgg agttgaagac tccagcctcc tcaactgcgc
     1801 cccccataag ctccgggccc ggggcgctgg cctctgtgcc cgcctctcac ccggcacacg
     1861 gcttggcacc ccacgagtcc cagctgcacc tgaaagggga cccccactac tccttcaacc
     1921 acccgttctc catcaacaac ctcatgtcct cctcggagca gcagcataag ctggacttca
     1981 aggcatacga acaggcactg caatactcgc cttacggctc tacgttgccc gccagcctgc
     2041 ctctaggcag cgcctcggtg accaccagga gccccatcga gccctcagcc ctggagccgg
     2101 cgtactacca aggtgtgtat tccagacccg tcctaaacac ttccgctagc gagggcagag
     2161 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctggtacc gtagcaggtc
     2221 atgcctctgg cagccccgca ttcgggaccg cctctcattc gaattgcgaa catgaagaga
     2281 tccacctcgc cggctcgatc cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc
     2341 atcgcgtcat ccaggccagc gccaacgccg cggaatttct gaatctcgga agcgtactcg
     2401 gcgttccgct cgccgagatc gacggcgatc tgttgatcaa gatcctgccg catctcgatc
     2461 ccaccgccga aggcatgccg gtcgcggtgc gctgccggat cggcaatccc tctacggagt
     2521 actgcggtct gatgcatcgg cctccggaag gcgggctgat catcgaactc gaacgtgccg
     2581 gcccgtcgat cgatctgtca ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg
     2641 gttcactgcg cgcgctgtgc gatgacaccg tgctgctgtt tcagcagtgc accggctacg
     2701 accgggtgat ggtgtatcgt ttcgatgagc aaggccacgg cctggtattc tccgagtgcc
     2761 atgtgcctgg gctcgaatcc tatttcggca accgctatcc gtcgtcgact gtcccgcaga
     2821 tggcgcggca gctgtacgtg cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc
     2881 cggtgccgct ggagccgcgg ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct
     2941 gcttcctgcg ctcgatgtcg ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg
     3001 ccaccctggc ggtgtcgctg gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc
     3061 attatctgcc gcgcttcatc cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa
     3121 ggatcgcgac gcggatcacc gcgcttgaga gctaaaccgg ttagtaatga gtttaaacgg
     3181 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     3241 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg
     3301 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     3361 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     3421 caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta
     3481 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
     3661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3841 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3901 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4021 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4081 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4141 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4201 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4261 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     4321 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4381 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4441 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4501 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4561 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4621 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4681 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     4741 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     4801 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     4861 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     4921 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4981 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5041 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5101 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
     5161 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5221 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     5281 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5401 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     5461 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5521 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     5581 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5641 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5701 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5761 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5821 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5881 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5941 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6001 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6061 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6121 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6181 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6241 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6301 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6361 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6421 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6481 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6541 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6601 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6661 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6721 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6781 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6841 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6901 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6961 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7021 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7081 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7141 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7201 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7261 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7321 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7381 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7441 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7501 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7561 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7621 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat
     7681 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     7741 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     7801 cttagg
//
