LOCUS       Exported                7575 bp DNA     circular SYN 25-JAN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2022 from SnapGene Viewer 6.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..627
                     /label=CMV_promoter
                     /label=CMV_immearly_promoter
                     /note="color: #ffffff"
     gene            729..2144
                     /label=FOXA1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2151..2205
                     /label=T2A
                     /note="color: #a6acb3"
     gene            2211..2924
                     /label=EGFP
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      2948..3218
                     /label=TK_PA_terminator
                     /note="color: #ffffff"
     misc_feature    3272..3578
                     /label=f1_origin
                     /note="color: #a6acb3"
     promoter        3710..3978
                     /label=SV40_promoter
                     /note="color: #ffffff"
     gene            4096..4884
                     /label=NeoR/KanR
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      5066..5185
                     /label=SV40_PA_terminator
                     /note="color: #ffffff"
     misc_feature    complement(5620..6239)
                     /label=pBR322_origin
                     /note="color: #a6acb3; direction: LEFT"
     gene            complement(6394..7254)
                     /label=Ampicillin
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(7296..7324)
                     /label=AmpR_promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagaat gttaggaact gtgaagatgg aagggcatga aaccagcgac tggaacagct
      781 actacgcaga cacgcaggag gcctactcct ccgtcccggt cagcaacatg aactcaggcc
      841 tgggctccat gaactccatg aacacctaca tgaccatgaa caccatgact acgagcggca
      901 acatgacccc ggcgtccttc aacatgtcct atgccaaccc gggcctaggg gccggcctga
      961 gtcccggcgc agtagccggc atgccggggg gctcggcggg cgccatgaac agcatgactg
     1021 cggccggcgt gacggccatg ggtacggcgc tgagcccgag cggcatgggc gccatgggtg
     1081 cgcagcaggc ggcctccatg aatggcctgg gcccctacgc ggccgccatg aacccgtgca
     1141 tgagccccat ggcgtacgcg ccgtccaacc tgggccgcag ccgcgcgggc ggcggcggcg
     1201 acgccaagac gttcaagcgc agctacccgc acgccaagcc gccctactcg tacatctcgc
     1261 tcatcaccat ggccatccag caggcgccca gcaagatgct cacgctgagc gagatctacc
     1321 agtggatcat ggacctcttc ccctattacc ggcagaacca gcagcgctgg cagaactcca
     1381 tccgccactc gctgtccttc aatgactgct tcgtcaaggt ggcacgctcc ccggacaagc
     1441 cgggcaaggg ctcctactgg acgctgcacc cggactccgg caacatgttc gagaacggct
     1501 gctacttgcg ccgccagaag cgcttcaagt gcgagaagca gccgggggcc ggcggcgggg
     1561 gcgggagcgg aagcgggggc agcggcgcca agggcggccc tgagagccgc aaggacccct
     1621 ctggcgcctc taaccccagc gccgactcgc ccctccatcg gggtgtgcac gggaagaccg
     1681 gccagctaga gggcgcgccg gcccccgggc ccgccgccag cccccagact ctggaccaca
     1741 gtggggcgac ggcgacaggg ggcgcctcgg agttgaagac tccagcctcc tcaactgcgc
     1801 cccccataag ctccgggccc ggggcgctgg cctctgtgcc cgcctctcac ccggcacacg
     1861 gcttggcacc ccacgagtcc cagctgcacc tgaaagggga cccccactac tccttcaacc
     1921 acccgttctc catcaacaac ctcatgtcct cctcggagca gcagcataag ctggacttca
     1981 aggcatacga acaggcactg caatactcgc cttacggctc tacgttgccc gccagcctgc
     2041 ctctaggcag cgcctcggtg accaccagga gccccatcga gccctcagcc ctggagccgg
     2101 cgtactacca aggtgtgtat tccagacccg tcctaaacac ttccgctagc gagggcagag
     2161 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctggtacc gtgagcaagg
     2221 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2281 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2341 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2401 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2461 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2521 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2581 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2641 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2701 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2761 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2821 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2881 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaccggt tagtaatgag
     2941 tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     3001 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     3061 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     3121 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     3181 gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc
     3241 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3301 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3361 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt
     3421 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3481 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3541 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3601 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat
     3661 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     3721 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3781 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3841 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3901 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3961 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4021 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     4081 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4141 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4201 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4261 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4321 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4381 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4441 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4501 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4561 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4621 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4681 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4741 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4801 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4861 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
     4921 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4981 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5041 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5101 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5161 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5221 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5281 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5341 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5401 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5461 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5521 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5581 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5641 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5701 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5761 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5821 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5881 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5941 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6001 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6061 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6121 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7381 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
     7441 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     7501 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     7561 aagaatctgc ttagg
//
