LOCUS       __2_pMOD4_mT2A_r        3266 bp    DNA        circular     16-NOV-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 24, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    621..710
                     /note="mT2A"
                     /locus_tag="mT2A"
                     /label="mT2A"
                     /ApEinfo_label="mT2A"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    711..1633
                     /note="rpsL-bsd"
                     /locus_tag="rpsL-BSD"
                     /label="rpsL-BSD"
                     /ApEinfo_label="rpsL-BSD"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    849..1223
                     /note="rpsL"
                     /locus_tag="rpsL"
                     /label="rpsL"
                     /ApEinfo_label="rpsL"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    925..937
                     /note="missing an A"
                     /locus_tag="missing an A"
                     /label="missing an A"
                     /ApEinfo_label="missing an A"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1235..1633
                     /note="bsdR"
                     /locus_tag="BSD"
                     /label="BSD"
                     /ApEinfo_label="BSD"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(1672..1705)
                     /note="FRT"
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2333..2361
                     /locus_tag="AmpR_promoter"
                     /label="AmpR_promoter"
                     /ApEinfo_label="AmpR_promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2403..3263
                     /codon_start=1
                     /note="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     I
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     S
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     W
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     A
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     S LIKHW"
                     /locus_tag="bla"
                     /label="bla"
                     /ApEinfo_label="bla"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    711..848
                     /locus_tag="Promoter"
                     /label="Promoter"
                     /ApEinfo_label="Promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1706..1733
                     /locus_tag="3' homology - add PacI"
                     /label="3' homology - add PacI"
                     /ApEinfo_label="3' homology - add PacI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttgtg
       61 catccgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
      121 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
      181 taacgtacta agctctcatg gctaacgtac taagctctca tggctaacgt actaagctct
      241 catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag
      301 ttttataaga aaaaaaagaa tatataaggc ttttaaagct agcttttaag gtttcaccat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aagacagctg
      541 tctcttatac acatctcaac cctgaagctc ttgttggcta gtgcgtagtc gttggcaagc
      601 ttgcatgcct gcaggtcgac ggaggatctg gcggaagatc tgaaggccgc ggcagcctgc
      661 tgacatgcgg cgacgtggaa gagaaccctg gccctgctcc tggcagcaca ggcctggtga
      721 tgatggcggg atcgttgtat atttcttgac accttttcgg catcgcccta aaattcggcg
      781 tcctcatatt gtgtgaggac gttttattac gtgtttacga agcaaaagct aaaaccagga
      841 gctatttaat ggcaacagtt aaccagctgg tacgcaaacc acgtgctcgc aaagttgcga
      901 aaagcaacgt gcctgcgctg gaagcatgcc cgcaaaaacg tggcgtatgt actcgtgtat
      961 atactaccac tcctaaaaaa ccgaactccg cgctgcgtaa agtatgccgt gttcgtctga
     1021 ctaacggttt cgaagtgact tcctacatcg gtggtgaagg tcacaacctg caggagcact
     1081 ccgtgatcct gatccgtggc ggtcgtgtta aagacctccc gggtgttcgt taccacaccg
     1141 tacgtggtgc gcttgactgc tccggcgtta aagaccgtaa gcaggctcgt tccaagtatg
     1201 gcgtgaagcg tcctaaggct taaggaggac aatcatggcc aagcctttgt ctcaagaaga
     1261 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     1321 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     1381 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     1441 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     1501 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     1561 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     1621 gtgggagggc taaaagctag cttggctgga cgtaaactcc tcttcagacc tgaagttcct
     1681 atactttcta gagaatagga acttcggaat tcgatatcaa gcttatcgat accggatccc
     1741 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggacgaacta tacaaaagcc
     1801 cacaagcttt gggtaccgag ctcgaattcg agccaatatg cgagaacacc cgagaaaatt
     1861 catcgatgat ggttgagatg tgtataagag acagctgtcg taatagcgaa gaggcccgca
     1921 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     1981 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     2041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     2221 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     2281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2401 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2461 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2521 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2581 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2641 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2701 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2761 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2821 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2881 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2941 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3001 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3061 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3121 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3181 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3241 cctcactgat taagcattgg taactg   
//
