LOCUS       Exported                7526 bp DNA     circular SYN 25-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     enhancer        2420..2797
                     /label=hCMV
                     /note="hCMV"
     promoter        2803..4144
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     intron          3172..4096
                     /note="intron"
     gene            complement(4151..4184)
                     /label=loxP
                     /note="loxP"
     gene            4186..4251
                     /label=EM7 promoter
                     /note="EM7 promoter"
     CDS             4252..5055
                     /label=Neo
                     /note="Neo"
     gene            5130..5432
                     /label=bGHpA
                     /note="bGHpA"
     misc_feature    5438..5665
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5671..5898
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    5904..6131
                     /label=SV40 pA
                     /note="SV40 pA"
     gene            complement(6137..6170)
                     /label=loxP
                     /note="loxP"
     misc_feature    6210..6226
                     /label=misc_feature 2
     misc_feature    6238..6254
                     /label=misc_feature 3
     misc_feature    6266..6282
                     /label=misc_feature 4
     misc_feature    6294..6310
                     /label=misc_feature 1
     misc_feature    6333..6634
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    6333..6340
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    complement(6881..6902)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(6911..6927)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaaccggcgg atcacaagca ataataacct gtagttttgc
     2221 tgcataaaac cccagatgac tacctatcct cccattttcc ttatttgccc ctattaaaaa
     2281 acttcccgac aaaaccgaaa atctgtggga agtcttgtcc ctccaatttt acacctgttc
     2341 aattcccctg caggacaacg cccacacacc aggttagcct ttaagcctgc ccagaagact
     2401 cccgcccatc ttctaggtcg acattgatta ttgactagtt attaatagta atcaattacg
     2461 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2521 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2581 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2641 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2701 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2761 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     2821 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     2881 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg
     2941 gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc
     3001 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
     3061 cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc
     3121 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     3181 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     3241 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     3301 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     3361 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     3421 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     3481 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     3541 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     3601 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     3661 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg
     3721 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
     3781 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
     3841 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
     3901 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
     3961 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
     4021 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
     4081 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     4141 caaagaattc ataacttcgt atagcataca ttatacgaag ttatagttga caattaatca
     4201 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgggatcg
     4261 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4321 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4381 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4441 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4501 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4561 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4621 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4681 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4741 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     4801 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4861 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4921 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4981 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5041 gacgagttct tctgagggga tcaattcgct agcagatctc cctcactaaa gggaacaaaa
     5101 gctggagctc caccgcggtg gcggccgctc tagagctcgc tgatcagcct cgactgtgcc
     5161 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5221 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5281 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5341 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5401 ctggggctcg actagagctt gcggaaccct tagctagaat cataatcagc cttaccacat
     5461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5521 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5641 tgtccaaact catcaatgta tcttaactag aatcataatc agccatacca catttgtaga
     5701 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5761 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5821 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5881 actcatcaat gtatcttaac tagaatcata atcagccata ccacatttgt agaggtttta
     5941 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6001 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6121 aatgtatctt aactagataa cttcgtatag catacattat acgaagttat aggcgcgccc
     6181 atgttaatta agatcggatc cataatggca ttcaccgcgt gccttaattc gaatggcatt
     6241 caccgcgtgc cttaaacgcg ttggcattca ccgcgtgcct taaatgcatt ggcattcacc
     6301 gcgtgcctta acagatccac tagagcggcc gcctagagct cgctgatcag cctcgactgt
     6361 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6421 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6481 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6541 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6601 cagctggggc tcgactagag cttgcggaac ccttaagctt aaagactgga gttgcagatc
     6661 acgagggaag agggggaagg gattctccca ggcccagggc ggtcctcaga agccaggagg
     6721 cagcagagaa ctcccagaaa ggtattgcaa cactcccctc ccccctccgg agaagggtgc
     6781 ggccttctcc ccgcctactc cactgcagct cccttactga taacaactca gagcgacttt
     6841 gggagagcaa gtgctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt
     6901 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6961 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7021 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     7081 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7141 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7201 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7261 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7321 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7381 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7441 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7501 taatgcgccg ctacagggcg cgtcag
//
