LOCUS       EC00968_(L0-ttCPF1_8_in 6921 bp ds-DNA     circular     26-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             227..1237
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     exon            227..1237
                     /label="Translation 227-1237"
     mutation        1204..1204
                     /label="G\to\A"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1397..2011
                     /label="rep\(pMB1)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      complement(1996..1996)
                     /label="ORI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2244..6917
                     /label="ttAtCas12a with 8 introns"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2259..2287
                     /label="CPF_int_F1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2710..2712
                     /label="D156R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2770..2790
                     /label="CPF_int_F2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2821..2911
                     /label="intron\Gluc4"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3043..3067
                     /label="CPF_int_F3"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     intron          3173..3293
                     /label="intron\e2f58"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3501..3524
                     /label="CPF_int_F4"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3738..3838
                     /label="intron\cdpk3"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3953..3979
                     /label="CPF_int_F5"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4201..4321
                     /label="intron\chtog11"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4470..4492
                     /label="CPF_int_F6"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4746..4869
                     /label="intron\atbg11"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4975..4995
                     /label="CPF_int_F7"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     intron          5248..5356
                     /label="intron\atipl3"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5501..5520
                     /label="CPF_int_F8"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5723..5872
                     /label="intron"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5978..6001
                     /label="CPF_int_F9"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6504..6532
                     /label="CPF_int_F10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6632..6752
                     /label="Intron\Rlk7"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6915..6917
                     /label="Stop"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
       61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      421 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
      481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
      601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     1201 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     1261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2101 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2161 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2221 tcattaatca ctctgtggtc tcaaatgagc aagctcgaga agtttaccaa ctgctacagc
     2281 ctctctaaga ccctcaggtt caaggctatc cctgtgggaa agacccaaga gaatatcgac
     2341 aacaagaggc tcctcgtcga ggatgagaag agagctgaag attacaaggg cgtgaagaag
     2401 ctcctcgaca ggtactacct cagcttcatc aacgatgtgc tccacagcat caagctcaag
     2461 aacctcaaca actacatcag cctcttccgt aagaaaacca ggaccgagaa agagaacaaa
     2521 gagcttgaga acctcgagat caacctccgt aaagagatcg ccaaggcttt caagggaaac
     2581 gagggataca agagcctctt caagaaggat attatcgaga caatcctgcc tgagttcctg
     2641 gacgataagg atgagatcgc tctcgtgaac agcttcaacg gattcactac tgccttcacc
     2701 ggattcttca gaaacaggga aaacatgttc agcgaagagg ccaagagcac ctctatcgct
     2761 ttcagatgca tcaacgagaa cctcacgcgt tacatcagca acatggacat cttcgagaag
     2821 gtaacattcc ttagttacct ttcttttctt tttccatcat aagtttatag attgtacatg
     2881 ctttgagatt tttctttgca aacaatctca ggtggacgcc atcttcgata agcacgaggt
     2941 gcaagaaatc aaagagaaga tcctcaacag cgactacgac gtcgaggact tttttgaagg
     3001 ggagttcttc aacttcgttc tcacccaaga gggcatcgac gtgtacaacg ctattatcgg
     3061 aggattcgtg accgagtctg gggagaagat taagggactc aacgagtaca tcaacctgta
     3121 caaccagaaa acgaagcaga agctcccgaa gttcaagccg ctctacaagc aggtctgtct
     3181 ttcctatttc atatgtttaa tcctaggaat ttgatcaatt gattgtatgt atgtcgatcc
     3241 caagactttc ttgttcactt atatcttaac tctctctttg ctgtttcttg caggttctct
     3301 ctgatcgtga gagcctctca ttttacggtg agggttacac ctctgacgag gaagtgcttg
     3361 aggttttccg taacaccctc aacaagaaca gcgagatctt ctcgtccatc aagaagttgg
     3421 agaagctttt caagaacttc gacgagtaca gcagcgctgg gatcttcgtt aagaacggac
     3481 ctgctatcag caccatcagc aaggatattt tcggcgagtg gaacgtgatc agggacaagt
     3541 ggaatgctga gtacgatgac atccacctca agaagaaggc tgtcgtcact gagaagtacg
     3601 aggatgacag gcgtaagtcg ttcaagaaga tcggctcttt cagcctcgag cagcttcaag
     3661 aatacgctga tgctgatctc agcgtggtcg agaagctcaa agagatcatc atccagaagg
     3721 tcgacgagat ctacaaggta agttgttact tatgattgtt ttcctctctg ctacatgtat
     3781 tttgttgttc atttctgtaa gatataagaa ttgagttttc ctctgatgat attattaggt
     3841 gtacgggtcc tctgagaagt tgttcgatgc tgatttcgtc ctcgagaaga gtctgaagaa
     3901 gaacgacgct gtcgtcgcga tcatgaagga tttgctcgac agcgtgaagt ccttcgagaa
     3961 ctatatcaag gccttcttcg gagagggcaa agagactaat agggacgagt ctttctacgg
     4021 ggatttcgtg ctcgcttacg atatcctcct caaggtggac catatctacg acgccatcag
     4081 aaactacgtg acccagaagc cttacagcaa ggacaagttc aagttgtact ttcagaaccc
     4141 gcagttcatg ggcggatggg acaaagacaa agagacagat tacagggcca ccatcctcag
     4201 gttagtatca tatgaagaaa tacctagttt cagttgatga atgctatttt ctgacctcag
     4261 ttgttctctt ttgagaatta tttcttttct aatttgcctg atttttctat taattcatta
     4321 ggtacgggtc taagtactac ctggccatca tggacaagaa atacgccaag tgcctccaaa
     4381 agatcgacaa ggatgacgtg aacgggaact atgagaagat caactacaag ctccttccgg
     4441 gaccgaacaa gatgcttcct aaggtgttct tcagcaagaa atggatggcc tactacaacc
     4501 cgtctgagga catccagaaa atctacaaga acgggacctt caagaaaggc gacatgttca
     4561 acctcaacga ctgccacaag ctcatcgatt tcttcaagga cagcatctcg cgttacccga
     4621 agtggtctaa cgcttacgac tttaacttca gcgagacaga aaagtacaag gatatcgccg
     4681 ggttctaccg tgaggttgag gaacagggtt acaaAgttag cttcgagagc gcctccaaga
     4741 aagaggtaaa tcctggtcca cacttttacg ataaaaacac aagattttaa actatgaact
     4801 gatcaataat cattcctaaa agaccacact tttgttttgt ttctaaagta atttttactg
     4861 ttataacagg ttgacaagtt ggtcgaagag ggcaagctct acatgttcca gatctataac
     4921 aaggacttct ccgacaagag ccacggaact cctaacctcc atacgatgta cttcaagctg
     4981 cttttcgacg agaacaacca cgggcagatc agactttctg gtggtgctga actcttcatg
     5041 cgtagggcct cactcaagaa agaagagttg gttgttcacc cggccaactc tccaatcgct
     5101 aacaagaatc ctgacaaccc gaaaaagacc accacgctgt cttacgacgt ctacaaggac
     5161 aaaaggttca gcgaggacca gtacgagctt catatcccga tcgctatcaa caagtgcccg
     5221 aagaacatct tcaagatcaa taccgaggta aggacttctc atgaatatta gtggcagatt
     5281 agtgttgtta aagtctttgg ttagataatc gatgcctcct aattgtccat gttttactgg
     5341 ttttctacaa ttaaaggtga gggtgctgct caagcacgat gataaccctt acgtgatcgg
     5401 aatcgatcgt ggtgagagaa acctcctcta catcgttgtg gtggacggaa agggaaacat
     5461 cgtcgagcag tacagcctga acgagattat caacaatttc aacggcatca ggatcaagac
     5521 cgactaccac tcactcctcg ataagaaaga aaaagagcgt ttcgaggcca ggcagaactg
     5581 gacttctatc gaaaacatca aagagttgaa ggccggctac atctctcagg tggtgcataa
     5641 gatctgcgag ctggtggaaa agtacgatgc tgtgatcgct cttgaggacc tcaactctgg
     5701 gttcaagaac agtagagtga aggtaagttc tgcatttggt tatgctcctt gcattttagg
     5761 tgttcgtcgc acttccattt ccatgaatag ctaagatttt ttttctctgc attcattctt
     5821 cttgcctcag ttctaactgt ttgtggtatt tttgttttaa ttattgctac aggttgagaa
     5881 gcaggtctac caaaagttcg agaagatgct catcgacaag ctcaactaca tggtggacaa
     5941 aaagagcaac ccttgcgcta ccggtggtgc tcttaaggga taccagatca cgaacaagtt
     6001 cgagtccttc aagagcatga gcacccagaa cggcttcatc ttctatatcc ctgcttggct
     6061 caccagcaag atcgatcctt ctactggttt cgtgaacctg ctcaagacca agtacacctc
     6121 gatcgccgac agcaagaagt tcatctcgtc tttcgacagg atcatgtacg tgccggaaga
     6181 ggatcttttc gagttcgctc tcgactataa gaacttcagc aggaccgacg ccgactacat
     6241 taagaagtgg aagctctact cctacgggaa ccgtatcagg atcttccgaa atccgaagaa
     6301 aaacaacgtg ttcgactggg aagaagtgtg cctcacctct gcctacaaag aactgttcaa
     6361 caagtacggc atcaactacc agcagggtga tatcagggct cttttgtgcg agcagagcga
     6421 caaggcattc tacagctcat tcatggccct catgtctctc atgctccaga tgaggaactc
     6481 tatcaccgga aggaccgatg tggacttcct tatctctccg gtcaagaact ctgacgggat
     6541 cttctacgac agccgtaact atgaggctca agagaacgct atcctgccga agaatgctga
     6601 tgcaaacggg gcttacaaca ttgcgagaaa ggtaaagcaa ctgtgtttta atcaatttct
     6661 tgtcaggata tatggattat aacttaattt ttgagaaatc tgtagtattt ggcgtgaaat
     6721 gagtttgctt tttggtttct cccgtgttat aggttctctg ggctatcggg cagtttaaga
     6781 aagcggaaga tgagaagctg gacaaggtga agatcgccat ctccaacaaa gagtggcttg
     6841 agtacgctca gacctccgtt aagcacaaga ggcctgctgc tactaagaaa gctggtcagg
     6901 ctaagaagaa gaaatgagct t
//