LOCUS       Exported                9878 bp DNA     circular SYN 21-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(10..1152)
                     /label=deltaVP1
     promoter        1162..1829
                     /label=GAL10/GAL1 bi-directional promoter
                     /note="Obtained from plasmid pILGFPB5A"
     protein_bind    1497..1614
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     gene            1839..2036
                     /label=VP2C
     CDS             2043..3101
                     /codon_start=1
                     /label=ERG20 (F96W-N127W)
                     /translation="MASEKEIRRERFLNVFPKLVEELNASLLAYGMPKEACDWYAHSLN
                     YNTPGGKLNRGLSVVDTYAILSNKTVEQLGQEEYEKVAILGWCIELLQAYWLVADDMMD
                     KSITRRGQPCWYKVPEVGEIAIWDAFMLEAAIYKLLKSHFRNEKYYIDITELFHEVTFQ
                     TELGQLMDLITAPEDKVDLSKFSLKKHSFIVTFKTAYYSFYLPVALAMYVAGITDEKDL
                     KQARDVLIPLGEYFQIQDDYLDCFGTPEQIGKIGTDIQDNKCSWVINKALELASAEQRK
                     TLDENYGKKDSVAEAKCKKIFNDLKIEQLYHEYEESIAKDLKAKISQVDESRGFKADVL
                     TAFLNKVYKRSK"
     misc_feature    3108..3832
                     /label=tGAL10-pGAL7
                     /note="GAL10 terminator - GAL7 promoterFrom S288C genomic
                     DNA
                     "
     gene            3842..4039
                     /label=VP2C
     CDS             4046..5767
                     /codon_start=1
                     /product="Actinidia chinensis nerolidol/linalool synthase
                     Codon optimized for expression in Saccharomyces cerevisae "
                     /label=AcNES1
                     /translation="MATAAGPIATNNSPQNSNAYRTPIAPSVPITHKWSIAEDLTCISN
                     PSKHNNPQTGYRSFSDELYVKYEEKLEDVRKALREVEENPLEGLVMIDALQRLGIDYHF
                     RGEIGAFLQKQQIISSTPDGYPEHGLYEVSTLFRFLRQEGHNVTADVFNNFKDKEGRFR
                     SELSTDIRGLMSLYEASQLRIEGEDILDQAADFSSQLLGRWTKDPNHHEARLVSNTLTH
                     PYHKSLATFMGQKLSYMNCKGPNWDGVDNLQELAKMDLTIVQSIHQKEVFQVSQWWKDT
                     GLANELKLARNQPLKWYMWPMAALTDPRFSEERVELTKPISFIYIIDDIFDVYGTIEEL
                     TLFTDAVNRWELSAVEQLPDYMKVCFKALYDVTNEIAYKIYKKHGQNPIDSLQKTWASL
                     CNAFLVEAKWFASGHLPNAEEYLKNGIISSGVHVVLAHMFFLLGDGITQESVDLVDDYP
                     GISTSIATILRLSDDLGSAKDEDQDGYDGSYIECYMKEHKGSSVDSAREEVIRMISEAW
                     KCLNKECLSPNPFSESFRIGSLNMARMIPMMYSYDDNHNLPILEEHMKAMIYNTSL"
     terminator      5774..5918
                     /label=HIS5 terminator
     terminator      5930..6047
                     /label=K. lactis URA3 terminator
     terminator      5930..6047
                     /label=K. lactis URA3 terminator
     gene            complement(6048..6851)
                     /product="Kluyveromyces lactis URA3"
                     /label=K. lactis URA3
     misc_feature    6852..7024
                     /label=S. cerevisiae URA3 promoter
     promoter        7518..7622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7623..8483
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8654..9242
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9530..9551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9566..9596
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9604..9620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      join(9645..9878,1)
                     /label=S. cerevisiae URA3 terminator
ORIGIN
        1 tgcggccgct taattaccag ggaatacagt tttagtttta ccgaatctat ctacatatct
       61 tgtcatgtca gggtcacctg gaactggttc agtaccatcg taaactctaa cttcttcaac
      121 ttgtgtgttt tcaccttcca ttggttgacc ttgaacttgt ggtaacatat tattaaacaa
      181 agaagaaatc aaagaagcca ttgggtatgg atttttaacc catctttttc ttaaagtaat
      241 cttgaagtat cttggcaaac ctctccaatg atgaacatcg tagtttcttg taactctcca
      301 acccataata tcaacgcaag ataagtacaa accttcacct ttacataatg gaccaacacc
      361 attttcatct aacaaaacag ttgtcaaagt gttagtgaat tgcaaaactg gtggagttgt
      421 agtaccacca gtgtaattac cgaagtatct tgtgttttca tttttagctg gatctggatg
      481 ccaaatttca actgggtaca tgccatcctt atccaattta gcctttgaaa ttgggttcaa
      541 aacttgatcc ttattaacca tatctttctt agtgattgtt ttaatagtaa caacaccttc
      601 ttccttgtac ttagttcttg catctgtaac taaaccttgc aaatccaatg gttcaccacc
      661 aacagcaaaa acatgatatt gtgaaccttc aactggagta gaaataccct ttgtgttaac
      721 agtatctgtt ggcttattaa aaccatgaac atccaacaaa gaaccagaac caacaacttc
      781 tgttttaact gaaactgctt cccacatttg caaagtatca caagtcaaat cttcgttcaa
      841 cattggtaat tgcaattttg ccatagacca agttggtaat gtattattac ctggtgaatc
      901 ttcagtatca gatgtagcca aattaatacc tcttgaccaa ccgtaatatt gaccaccttc
      961 agttaaagat tctggtgttg gtggttgacc cattcttggg ttcaaaaatg cttcgatttc
     1021 agtaacagaa tctggacctg taaccaaatc caaaacttcc ataccacctt taatcaataa
     1081 cttaggaact ggagctggtc tagggcaagc ctttgtgcat ttggtttcgc atttacttac
     1141 acctgaagcc attttgctag ctatagtttt ttctccttga cgttaaagta tagaggtata
     1201 ttaacaattt tttgttgata cttttatgac atttgaataa gaagtaatac aaactgaaaa
     1261 tgttgaaagt attagttaaa gtggttatgc agcttttcca tttatatatc tgttaataga
     1321 tcaaaaatca tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca
     1381 ttatcatcct atggttgtta atttgattcg ttaatttgaa ggtttgtggg gccaggttac
     1441 tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga
     1501 gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca
     1561 cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa tccgtacttc
     1621 aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg
     1681 ctaagataat ggggctcttt acatttccac aacatataag taagattaga tatggatatg
     1741 tatatggtgg taatgccatg taatatgatt attaaacttc tttgcgtcca tccaaaaaaa
     1801 aagtaagaat ttttgaaaat tcaatataaa tcgataaaat gggtaatggt ggtccaactc
     1861 cagctgctca tattcaagat gaatctggtg aagttattaa attttatcaa gctcaagttg
     1921 tttctcatca aagagttact ccagattgga tgttgccatt gattttgggt ttgtatggtg
     1981 acatcactcc aggtggtggt ggttctggtg gcggtggctc cggtggcggc ggttctggat
     2041 ccatggcttc agaaaaagaa attaggagag agagattctt gaacgttttc cctaaattag
     2101 tagaggaatt gaacgcatcg cttttggctt acggtatgcc taaggaagca tgtgactggt
     2161 atgcccactc attgaactac aacactccag gcggtaagct aaatagaggt ttgtccgttg
     2221 tggacacgta tgctattctc tccaacaaga ccgttgaaca attggggcaa gaagaatacg
     2281 aaaaggttgc cattctaggt tggtgcattg agttgttgca ggcttactgg ttggtcgccg
     2341 atgatatgat ggacaagtcc attaccagaa gaggccaacc atgttggtac aaggttcctg
     2401 aagttgggga aattgccatc tgggacgcat tcatgttaga ggctgctatc tacaagcttt
     2461 tgaaatctca cttcagaaac gaaaaatact acatagatat caccgaattg ttccatgagg
     2521 tcaccttcca aaccgaattg ggccaattga tggacttaat cactgcacct gaagacaaag
     2581 tcgacttgag taagttctcc ctaaagaagc actccttcat agttactttc aagactgctt
     2641 actattcttt ctacttgcct gtcgcattgg ccatgtacgt tgccggtatc acggatgaaa
     2701 aggatttgaa acaagccaga gatgtcttga ttccattggg tgaatacttc caaattcaag
     2761 atgactactt agactgcttc ggtaccccag aacagatcgg taagatcggt acagatatcc
     2821 aagataacaa atgttcttgg gtaatcaaca aggcattaga acttgcttcc gcagaacaaa
     2881 gaaagacttt agacgaaaat tacggtaaga aggactcagt cgcagaagcc aaatgcaaaa
     2941 agattttcaa tgacttgaaa attgaacagc tataccacga atatgaagag tctattgcca
     3001 aggatttgaa ggccaaaatt tctcaggtcg atgagtctcg tggcttcaaa gctgatgtct
     3061 taactgcgtt cttgaacaaa gtttacaaga gaagcaaata aagatctttt gccagcttac
     3121 tatccttctt gaaaatatgc actctatatc ttttagttct taattgcaac acatagattt
     3181 gctgtataac gaattttatg ctatttttta aatttggagt tcagtgataa aagtgtcaca
     3241 gcgaatttcc tcacatgtag ggaccgaatt gtttacaagt tctctgtacc accatggaga
     3301 catcaaaaat tgaaaatcta tggaaagata tggacggtag caacaagaat atagcacgag
     3361 ccgcggagtt catttcgtta cttttgatat cactcacaac tattgcgaag cgcttcagtg
     3421 aaaaaatcat aaggaaaagt tgtaaatatt attggtagta ttcgtttggt aaagtagagg
     3481 gggtaatttt tcccctttat tttgttcata cattcttaaa ttgctttgcc tctccttttg
     3541 gaaagctata cttcggagca ctgttgagcg aaggctcatt agatatattt tctgtcattt
     3601 tccttaaccc aaaaataagg gaaagggtcc aaaaagcgct cggacaactg ttgaccgtga
     3661 tccgaaggac tggctataca gtgttcacaa aatagccaag ctgaaaataa tgtgtagcta
     3721 tgttcagtta gtttggctag caaagatata aaagcaggtc ggaaatattt atgggcatta
     3781 ttatgcagag catcaacatg ataaaaaaaa acagttgaat attccctcaa aaatcgataa
     3841 aatgggtaat ggtggtccaa ctccagctgc tcatattcaa gatgaatctg gtgaagttat
     3901 taaattttat caagctcaag ttgtttctca tcaaagagtt actccagatt ggatgttgcc
     3961 attgattttg ggtttgtatg gtgacatcac tccaggtggt ggtggttctg gtggcggtgg
     4021 ctccggtggc ggcggttctg agctcatggc taccgcagca ggtcctatcg caactaacaa
     4081 ctccccacaa aactccaacg cttacagaac tccaatcgct ccttccgtac caattactca
     4141 taaatggtct atagctgaag atttgacatg tatttccaat cctagtaagc acaataaccc
     4201 tcaaactggt tacagatcat tttctgacga attatacgtt aagtacgaag aaaagttgga
     4261 agatgttaga aaagcattaa gagaagttga agaaaaccct ttggaaggtt tagttatgat
     4321 agacgctttg caaagattgg gtatcgatta ccatttcaga ggtgaaattg gtgcattctt
     4381 gcaaaagcaa caaatcatat cttcaactcc agatggttac cctgaacatg gtttgtacga
     4441 agtttcaaca ttgtttagat tcttaagaca agaaggtcac aatgttaccg ctgacgtctt
     4501 taataacttc aaggataagg aaggtagatt cagatcagaa ttgtcaacag atattagagg
     4561 tttgatgtcc ttatacgaag caagtcaatt gagaatagaa ggtgaagaca tcttagatca
     4621 agctgctgat ttctccagtc aattgttagg tagatggaca aaagatccta atcatcacga
     4681 agccagattg gtttctaaca ctttaacaca tccataccac aagtcattgg ctacctttat
     4741 gggtcaaaaa ttgtcctaca tgaactgcaa gggtccaaac tgggacggtg tcgataattt
     4801 gcaagaatta gctaagatgg atttgactat cgtacaaagt atccatcaaa aggaagtatt
     4861 ccaagtttct caatggtgga aggatacagg tttagccaat gaattgaaat tggctagaaa
     4921 ccaaccattg aagtggtata tgtggcctat ggccgcttta accgatccaa gattcagtga
     4981 agaaagagtt gaattaacta agcctatttc ttttatatac atcatagatg acatcttcga
     5041 cgtctatggt accattgaag aattgacctt gtttactgat gcagttaata gatgggaatt
     5101 gtctgccgtc gaacaattac cagactacat gaaagtatgt ttcaaggctt tgtacgatgt
     5161 taccaacgaa atcgcataca aaatctataa aaagcatggt caaaacccta ttgattcctt
     5221 gcaaaagact tgggctagtt tgtgcaatgc atttttagtt gaagcaaagt ggttcgcctc
     5281 tggtcacttg ccaaatgcag aagaatactt aaagaacggt atcatctctt caggtgttca
     5341 tgttgtcttg gcccacatgt ttttcttgtt aggtgacggt attacacaag aatcagttga
     5401 tttggtagat gactatccag gtatttccac aagtatcgca accattttga gattatctga
     5461 tgacttgggt tcagccaaag atgaagacca agatggttat gatggttctt acatcgaatg
     5521 ttacatgaag gaacataagg gttccagtgt cgattcagcc agagaagaag taataagaat
     5581 gatctccgaa gcatggaaat gtttgaataa ggaatgctta tcaccaaacc ctttttctga
     5641 atcattcaga ataggttcct tgaatatggc tagaatgatc cctatgatgt actcttacga
     5701 tgacaaccat aacttgccaa ttttagaaga acacatgaag gcaatgatct ataacacatc
     5761 tttataactc gagatagatt aatttaaaca gtatatgtac agttttatat atatatatat
     5821 atatatatac atatataaag aaacctgtgc gttttttgta ttttcaaata catttagttt
     5881 tggcgccact tctataaaag gtctcatgat attgttactc tagagatccc aatacaacag
     5941 atcacgtgat cttttgtaag atgaagttga agtgagtgtt gcaccgtgcc aatgcaggtg
     6001 gctattagat taaatatgtg atttgttcta ttaagtttcc tgtataatta atggggagcg
     6061 ctgattctct tttggtacgc ttcccatcca gcatttctgt atctttcacc ttcaacctta
     6121 ggatctctac ccttggcgaa aagtcctctg ccaacaatga tgatatctga tccaccactt
     6181 acaacttcgt cgacggttct gtactgctga cccaatgcat cgcctttgtc gtctaaacct
     6241 acacctgggg tcatgattag ccaatcaaac ccttcttctc ttcctcccat atcgttctga
     6301 gcaatgaacc caataacgaa atctttatca ctctttgcaa tatcaacggt acccttagta
     6361 tattcaccgt gtgctagaga acccttggaa gacaattcag caagcatcaa taatcccctt
     6421 ggttctttgg tgacctcttg cgcaccttgt ttcaagccag caacaatacc agcaccagta
     6481 accccgtggg cgttggtgat atcagaccat tctgcgatac ggtaaacgcc cgatgtatat
     6541 tgtaatttga ctgtgttacc gatatcggcg aattttctgt cctcaaatat caagaacttg
     6601 tatttctctg ccaatgcttt caatggaacg acagtaccct cataactgaa atcatccaag
     6661 atatcaacgt gtgttttcaa aaggcaaatg tatggaccca acgtttcaac aagtttcaat
     6721 agctcatcag tcgaacgaac gtcaagagaa gcacacaaat tggtcttctt ttcatccatt
     6781 aaacgtaaaa gtttcgatgc aaccggactt gcatgagtct cagctctact ggtatatgat
     6841 tttgtggaca tgttttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag
     6901 aatactgggc aatttcatgt ttcttcaaca ccacatatgc gtatatatac caatctaagt
     6961 ctgtgctcct tccttcgttc ttccttctgc tcggagatta ccgaatcaaa aaaatttaaa
     7021 tgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7081 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7141 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     7201 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     7261 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7321 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7381 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7441 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7861 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8761 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9661 ttgcatgcat ttaaatcatt acgaccgaga ttcccgggta ataactgata taattaaatt
     9721 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
     9781 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
     9841 accttagcat cccttccctt tgcaaatagt cctactag
//
