LOCUS       Exported                8310 bp DNA     circular SYN 21-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        2..669
                     /label=GAL10/GAL1 bi-directional promoter
                     /note="Obtained from plasmid pILGFPB5A"
     protein_bind    337..454
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             679..1737
                     /codon_start=1
                     /label=ERG20 (F96W-N127W)
                     /translation="MASEKEIRRERFLNVFPKLVEELNASLLAYGMPKEACDWYAHSLN
                     YNTPGGKLNRGLSVVDTYAILSNKTVEQLGQEEYEKVAILGWCIELLQAYWLVADDMMD
                     KSITRRGQPCWYKVPEVGEIAIWDAFMLEAAIYKLLKSHFRNEKYYIDITELFHEVTFQ
                     TELGQLMDLITAPEDKVDLSKFSLKKHSFIVTFKTAYYSFYLPVALAMYVAGITDEKDL
                     KQARDVLIPLGEYFQIQDDYLDCFGTPEQIGKIGTDIQDNKCSWVINKALELASAEQRK
                     TLDENYGKKDSVAEAKCKKIFNDLKIEQLYHEYEESIAKDLKAKISQVDESRGFKADVL
                     TAFLNKVYKRSK"
     misc_feature    1744..2468
                     /label=tGAL10-pGAL7
                     /note="GAL10 terminator - GAL7 promoterFrom S288C genomic
                     DNA
                     "
     CDS             2478..4199
                     /codon_start=1
                     /product="Actinidia chinensis nerolidol/linalool synthase
                     Codon optimized for expression in Saccharomyces cerevisae "
                     /label=AcNES1
                     /translation="MATAAGPIATNNSPQNSNAYRTPIAPSVPITHKWSIAEDLTCISN
                     PSKHNNPQTGYRSFSDELYVKYEEKLEDVRKALREVEENPLEGLVMIDALQRLGIDYHF
                     RGEIGAFLQKQQIISSTPDGYPEHGLYEVSTLFRFLRQEGHNVTADVFNNFKDKEGRFR
                     SELSTDIRGLMSLYEASQLRIEGEDILDQAADFSSQLLGRWTKDPNHHEARLVSNTLTH
                     PYHKSLATFMGQKLSYMNCKGPNWDGVDNLQELAKMDLTIVQSIHQKEVFQVSQWWKDT
                     GLANELKLARNQPLKWYMWPMAALTDPRFSEERVELTKPISFIYIIDDIFDVYGTIEEL
                     TLFTDAVNRWELSAVEQLPDYMKVCFKALYDVTNEIAYKIYKKHGQNPIDSLQKTWASL
                     CNAFLVEAKWFASGHLPNAEEYLKNGIISSGVHVVLAHMFFLLGDGITQESVDLVDDYP
                     GISTSIATILRLSDDLGSAKDEDQDGYDGSYIECYMKEHKGSSVDSAREEVIRMISEAW
                     KCLNKECLSPNPFSESFRIGSLNMARMIPMMYSYDDNHNLPILEEHMKAMIYNTSL"
     terminator      4206..4350
                     /label=HIS5 terminator
     terminator      4362..4479
                     /label=K. lactis URA3 terminator
     terminator      4362..4479
                     /label=K. lactis URA3 terminator
     gene            complement(4480..5283)
                     /product="Kluyveromyces lactis URA3"
                     /label=K. lactis URA3
     misc_feature    5284..5456
                     /label=S. cerevisiae URA3 promoter
     promoter        5950..6054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6055..6915
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7086..7674
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7962..7983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7998..8028
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8036..8052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      join(8077..8310,1)
                     /label=S. cerevisiae URA3 terminator
ORIGIN
        1 ttatagtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata
       61 cttttatgac atttgaataa gaagtaatac aaactgaaaa tgttgaaagt attagttaaa
      121 gtggttatgc agcttttcca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
      181 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta
      241 atttgattcg ttaatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat
      301 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca
      361 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccgtcg
      421 gagggctgtc gcccgctcgg cggcttctaa tccgtacttc aatatagcaa tgagcagtta
      481 agcgtattac tgaaagttcc aaagagaagg tttttttagg ctaagataat ggggctcttt
      541 acatttccac aacatataag taagattaga tatggatatg tatatggtgg taatgccatg
      601 taatatgatt attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat
      661 tcaatataag gatccaaaat ggcttcagaa aaagaaatta ggagagagag attcttgaac
      721 gttttcccta aattagtaga ggaattgaac gcatcgcttt tggcttacgg tatgcctaag
      781 gaagcatgtg actggtatgc ccactcattg aactacaaca ctccaggcgg taagctaaat
      841 agaggtttgt ccgttgtgga cacgtatgct attctctcca acaagaccgt tgaacaattg
      901 gggcaagaag aatacgaaaa ggttgccatt ctaggttggt gcattgagtt gttgcaggct
      961 tactggttgg tcgccgatga tatgatggac aagtccatta ccagaagagg ccaaccatgt
     1021 tggtacaagg ttcctgaagt tggggaaatt gccatctggg acgcattcat gttagaggct
     1081 gctatctaca agcttttgaa atctcacttc agaaacgaaa aatactacat agatatcacc
     1141 gaattgttcc atgaggtcac cttccaaacc gaattgggcc aattgatgga cttaatcact
     1201 gcacctgaag acaaagtcga cttgagtaag ttctccctaa agaagcactc cttcatagtt
     1261 actttcaaga ctgcttacta ttctttctac ttgcctgtcg cattggccat gtacgttgcc
     1321 ggtatcacgg atgaaaagga tttgaaacaa gccagagatg tcttgattcc attgggtgaa
     1381 tacttccaaa ttcaagatga ctacttagac tgcttcggta ccccagaaca gatcggtaag
     1441 atcggtacag atatccaaga taacaaatgt tcttgggtaa tcaacaaggc attagaactt
     1501 gcttccgcag aacaaagaaa gactttagac gaaaattacg gtaagaagga ctcagtcgca
     1561 gaagccaaat gcaaaaagat tttcaatgac ttgaaaattg aacagctata ccacgaatat
     1621 gaagagtcta ttgccaagga tttgaaggcc aaaatttctc aggtcgatga gtctcgtggc
     1681 ttcaaagctg atgtcttaac tgcgttcttg aacaaagttt acaagagaag caaataaaga
     1741 tcttttgcca gcttactatc cttcttgaaa atatgcactc tatatctttt agttcttaat
     1801 tgcaacacat agatttgctg tataacgaat tttatgctat tttttaaatt tggagttcag
     1861 tgataaaagt gtcacagcga atttcctcac atgtagggac cgaattgttt acaagttctc
     1921 tgtaccacca tggagacatc aaaaattgaa aatctatgga aagatatgga cggtagcaac
     1981 aagaatatag cacgagccgc ggagttcatt tcgttacttt tgatatcact cacaactatt
     2041 gcgaagcgct tcagtgaaaa aatcataagg aaaagttgta aatattattg gtagtattcg
     2101 tttggtaaag tagagggggt aatttttccc ctttattttg ttcatacatt cttaaattgc
     2161 tttgcctctc cttttggaaa gctatacttc ggagcactgt tgagcgaagg ctcattagat
     2221 atattttctg tcattttcct taacccaaaa ataagggaaa gggtccaaaa agcgctcgga
     2281 caactgttga ccgtgatccg aaggactggc tatacagtgt tcacaaaata gccaagctga
     2341 aaataatgtg tagctatgtt cagttagttt ggctagcaaa gatataaaag caggtcggaa
     2401 atatttatgg gcattattat gcagagcatc aacatgataa aaaaaaacag ttgaatattc
     2461 cctcaaaaga gctcaaaatg gctaccgcag caggtcctat cgcaactaac aactccccac
     2521 aaaactccaa cgcttacaga actccaatcg ctccttccgt accaattact cataaatggt
     2581 ctatagctga agatttgaca tgtatttcca atcctagtaa gcacaataac cctcaaactg
     2641 gttacagatc attttctgac gaattatacg ttaagtacga agaaaagttg gaagatgtta
     2701 gaaaagcatt aagagaagtt gaagaaaacc ctttggaagg tttagttatg atagacgctt
     2761 tgcaaagatt gggtatcgat taccatttca gaggtgaaat tggtgcattc ttgcaaaagc
     2821 aacaaatcat atcttcaact ccagatggtt accctgaaca tggtttgtac gaagtttcaa
     2881 cattgtttag attcttaaga caagaaggtc acaatgttac cgctgacgtc tttaataact
     2941 tcaaggataa ggaaggtaga ttcagatcag aattgtcaac agatattaga ggtttgatgt
     3001 ccttatacga agcaagtcaa ttgagaatag aaggtgaaga catcttagat caagctgctg
     3061 atttctccag tcaattgtta ggtagatgga caaaagatcc taatcatcac gaagccagat
     3121 tggtttctaa cactttaaca catccatacc acaagtcatt ggctaccttt atgggtcaaa
     3181 aattgtccta catgaactgc aagggtccaa actgggacgg tgtcgataat ttgcaagaat
     3241 tagctaagat ggatttgact atcgtacaaa gtatccatca aaaggaagta ttccaagttt
     3301 ctcaatggtg gaaggataca ggtttagcca atgaattgaa attggctaga aaccaaccat
     3361 tgaagtggta tatgtggcct atggccgctt taaccgatcc aagattcagt gaagaaagag
     3421 ttgaattaac taagcctatt tcttttatat acatcataga tgacatcttc gacgtctatg
     3481 gtaccattga agaattgacc ttgtttactg atgcagttaa tagatgggaa ttgtctgccg
     3541 tcgaacaatt accagactac atgaaagtat gtttcaaggc tttgtacgat gttaccaacg
     3601 aaatcgcata caaaatctat aaaaagcatg gtcaaaaccc tattgattcc ttgcaaaaga
     3661 cttgggctag tttgtgcaat gcatttttag ttgaagcaaa gtggttcgcc tctggtcact
     3721 tgccaaatgc agaagaatac ttaaagaacg gtatcatctc ttcaggtgtt catgttgtct
     3781 tggcccacat gtttttcttg ttaggtgacg gtattacaca agaatcagtt gatttggtag
     3841 atgactatcc aggtatttcc acaagtatcg caaccatttt gagattatct gatgacttgg
     3901 gttcagccaa agatgaagac caagatggtt atgatggttc ttacatcgaa tgttacatga
     3961 aggaacataa gggttccagt gtcgattcag ccagagaaga agtaataaga atgatctccg
     4021 aagcatggaa atgtttgaat aaggaatgct tatcaccaaa ccctttttct gaatcattca
     4081 gaataggttc cttgaatatg gctagaatga tccctatgat gtactcttac gatgacaacc
     4141 ataacttgcc aattttagaa gaacacatga aggcaatgat ctataacaca tctttataac
     4201 tcgagataga ttaatttaaa cagtatatgt acagttttat atatatatat atatatatat
     4261 acatatataa agaaacctgt gcgttttttg tattttcaaa tacatttagt tttggcgcca
     4321 cttctataaa aggtctcatg atattgttac tctagagatc ccaatacaac agatcacgtg
     4381 atcttttgta agatgaagtt gaagtgagtg ttgcaccgtg ccaatgcagg tggctattag
     4441 attaaatatg tgatttgttc tattaagttt cctgtataat taatggggag cgctgattct
     4501 cttttggtac gcttcccatc cagcatttct gtatctttca ccttcaacct taggatctct
     4561 acccttggcg aaaagtcctc tgccaacaat gatgatatct gatccaccac ttacaacttc
     4621 gtcgacggtt ctgtactgct gacccaatgc atcgcctttg tcgtctaaac ctacacctgg
     4681 ggtcatgatt agccaatcaa acccttcttc tcttcctccc atatcgttct gagcaatgaa
     4741 cccaataacg aaatctttat cactctttgc aatatcaacg gtacccttag tatattcacc
     4801 gtgtgctaga gaacccttgg aagacaattc agcaagcatc aataatcccc ttggttcttt
     4861 ggtgacctct tgcgcacctt gtttcaagcc agcaacaata ccagcaccag taaccccgtg
     4921 ggcgttggtg atatcagacc attctgcgat acggtaaacg cccgatgtat attgtaattt
     4981 gactgtgtta ccgatatcgg cgaattttct gtcctcaaat atcaagaact tgtatttctc
     5041 tgccaatgct ttcaatggaa cgacagtacc ctcataactg aaatcatcca agatatcaac
     5101 gtgtgttttc aaaaggcaaa tgtatggacc caacgtttca acaagtttca atagctcatc
     5161 agtcgaacga acgtcaagag aagcacacaa attggtcttc ttttcatcca ttaaacgtaa
     5221 aagtttcgat gcaaccggac ttgcatgagt ctcagctcta ctggtatatg attttgtgga
     5281 catgttttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     5341 gcaatttcat gtttcttcaa caccacatat gcgtatatat accaatctaa gtctgtgctc
     5401 cttccttcgt tcttccttct gctcggagat taccgaatca aaaaaattta aatgaattca
     5461 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5521 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5581 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     5641 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5701 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5761 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5821 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5881 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5941 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6001 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6061 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6121 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6181 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6241 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6301 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6361 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6421 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6481 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6541 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6601 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6661 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6721 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6781 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6841 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6901 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6961 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7021 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7081 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7141 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7201 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7261 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7321 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7381 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7441 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7501 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7561 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7621 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7681 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7741 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7801 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7861 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7921 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7981 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8041 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc
     8101 atttaaatca ttacgaccga gattcccggg taataactga tataattaaa ttgaagctct
     8161 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     8221 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     8281 atcccttccc tttgcaaata gtcctactag
//
