LOCUS       Exported                8970 bp DNA     circular SYN 21-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Institute for Molecular Bioscience
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            complement(10..1152)
                     /label=deltaVP1
     promoter        1162..1829
                     /label=GAL10/GAL1 bi-directional promoter
                     /note="Obtained from plasmid pILGFPB5A"
     protein_bind    1497..1614
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     gene            1839..2036
                     /label=VP2C
     CDS             2043..3098
                     /codon_start=1
                     /label=ERG20 (F96C)
                     /translation="MASEKEIRRERFLNVFPKLVEELNASLLAYGMPKEACDWYAHSLN
                     YNTPGGKLNRGLSVVDTYAILSNKTVEQLGQEEYEKVAILGWCIELLQAYCLVADDMMD
                     KSITRRGQPCWYKVPEVGEIAINDAFMLEAAIYKLLKSHFRNEKYYIDITELFHEVTFQ
                     TELGQLMDLITAPEDKVDLSKFSLKKHSFIVTFKTAYYSFYLPVALAMYVAGITDEKDL
                     KQARDVLIPLGEYFQIQDDYLDCFGTPEQIGKIGTDIQDNKCSWVINKALELASAEQRK
                     TLDENYGKKDSVAEAKCKKIFNDLKIEQLYHEYEESIAKDLKAKISQVDESRGFKADVL
                     TAFLNKVYKRSK"
     CDS             3138..4859
                     /codon_start=1
                     /product="Actinidia chinensis nerolidol/linalool synthase
                     Codon optimized for expression in Saccharomyces cerevisae "
                     /label=AcNES1
                     /translation="MATAAGPIATNNSPQNSNAYRTPIAPSVPITHKWSIAEDLTCISN
                     PSKHNNPQTGYRSFSDELYVKYEEKLEDVRKALREVEENPLEGLVMIDALQRLGIDYHF
                     RGEIGAFLQKQQIISSTPDGYPEHGLYEVSTLFRFLRQEGHNVTADVFNNFKDKEGRFR
                     SELSTDIRGLMSLYEASQLRIEGEDILDQAADFSSQLLGRWTKDPNHHEARLVSNTLTH
                     PYHKSLATFMGQKLSYMNCKGPNWDGVDNLQELAKMDLTIVQSIHQKEVFQVSQWWKDT
                     GLANELKLARNQPLKWYMWPMAALTDPRFSEERVELTKPISFIYIIDDIFDVYGTIEEL
                     TLFTDAVNRWELSAVEQLPDYMKVCFKALYDVTNEIAYKIYKKHGQNPIDSLQKTWASL
                     CNAFLVEAKWFASGHLPNAEEYLKNGIISSGVHVVLAHMFFLLGDGITQESVDLVDDYP
                     GISTSIATILRLSDDLGSAKDEDQDGYDGSYIECYMKEHKGSSVDSAREEVIRMISEAW
                     KCLNKECLSPNPFSESFRIGSLNMARMIPMMYSYDDNHNLPILEEHMKAMIYNTSL"
     terminator      4866..5010
                     /label=HIS5 terminator
     terminator      5022..5139
                     /label=K. lactis URA3 terminator
     terminator      5022..5139
                     /label=K. lactis URA3 terminator
     gene            complement(5140..5943)
                     /product="Kluyveromyces lactis URA3"
                     /label=K. lactis URA3
     misc_feature    5944..6116
                     /label=S. cerevisiae URA3 promoter
     promoter        6610..6714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6715..7575
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7746..8334
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8622..8643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8658..8688
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8696..8712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      join(8737..8970,1)
                     /label=S. cerevisiae URA3 terminator
ORIGIN
        1 tgcggccgct taattaccag ggaatacagt tttagtttta ccgaatctat ctacatatct
       61 tgtcatgtca gggtcacctg gaactggttc agtaccatcg taaactctaa cttcttcaac
      121 ttgtgtgttt tcaccttcca ttggttgacc ttgaacttgt ggtaacatat tattaaacaa
      181 agaagaaatc aaagaagcca ttgggtatgg atttttaacc catctttttc ttaaagtaat
      241 cttgaagtat cttggcaaac ctctccaatg atgaacatcg tagtttcttg taactctcca
      301 acccataata tcaacgcaag ataagtacaa accttcacct ttacataatg gaccaacacc
      361 attttcatct aacaaaacag ttgtcaaagt gttagtgaat tgcaaaactg gtggagttgt
      421 agtaccacca gtgtaattac cgaagtatct tgtgttttca tttttagctg gatctggatg
      481 ccaaatttca actgggtaca tgccatcctt atccaattta gcctttgaaa ttgggttcaa
      541 aacttgatcc ttattaacca tatctttctt agtgattgtt ttaatagtaa caacaccttc
      601 ttccttgtac ttagttcttg catctgtaac taaaccttgc aaatccaatg gttcaccacc
      661 aacagcaaaa acatgatatt gtgaaccttc aactggagta gaaataccct ttgtgttaac
      721 agtatctgtt ggcttattaa aaccatgaac atccaacaaa gaaccagaac caacaacttc
      781 tgttttaact gaaactgctt cccacatttg caaagtatca caagtcaaat cttcgttcaa
      841 cattggtaat tgcaattttg ccatagacca agttggtaat gtattattac ctggtgaatc
      901 ttcagtatca gatgtagcca aattaatacc tcttgaccaa ccgtaatatt gaccaccttc
      961 agttaaagat tctggtgttg gtggttgacc cattcttggg ttcaaaaatg cttcgatttc
     1021 agtaacagaa tctggacctg taaccaaatc caaaacttcc ataccacctt taatcaataa
     1081 cttaggaact ggagctggtc tagggcaagc ctttgtgcat ttggtttcgc atttacttac
     1141 acctgaagcc attttgctag ctatagtttt ttctccttga cgttaaagta tagaggtata
     1201 ttaacaattt tttgttgata cttttatgac atttgaataa gaagtaatac aaactgaaaa
     1261 tgttgaaagt attagttaaa gtggttatgc agcttttcca tttatatatc tgttaataga
     1321 tcaaaaatca tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca
     1381 ttatcatcct atggttgtta atttgattcg ttaatttgaa ggtttgtggg gccaggttac
     1441 tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga
     1501 gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca
     1561 cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa tccgtacttc
     1621 aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg
     1681 ctaagataat ggggctcttt acatttccac aacatataag taagattaga tatggatatg
     1741 tatatggtgg taatgccatg taatatgatt attaaacttc tttgcgtcca tccaaaaaaa
     1801 aagtaagaat ttttgaaaat tcaatataaa tcgataaaat gggtaatggt ggtccaactc
     1861 cagctgctca tattcaagat gaatctggtg aagttattaa attttatcaa gctcaagttg
     1921 tttctcatca aagagttact ccagattgga tgttgccatt gattttgggt ttgtatggtg
     1981 acatcactcc aggtggtggt ggttctggtg gcggtggctc cggtggcggc ggttctggat
     2041 ccatggcttc agaaaaagaa attaggagag agagattctt gaacgttttc cctaaattag
     2101 tagaggaatt gaacgcatcg cttttggctt acggtatgcc taaggaagca tgtgactggt
     2161 atgcccactc attgaactac aacactccag gcggtaagct aaatagaggt ttgtccgttg
     2221 tggacacgta tgctattctc tccaacaaga ccgttgaaca attggggcaa gaagaatacg
     2281 aaaaggttgc cattctaggt tggtgcattg agttgttgca ggcttactgt ttggtcgccg
     2341 atgatatgat ggacaagtcc attaccagaa gaggccaacc atgttggtac aaggttcctg
     2401 aagttgggga aattgccatc aatgacgcat tcatgttaga ggctgctatc tacaagcttt
     2461 tgaaatctca cttcagaaac gaaaaatact acatagatat caccgaattg ttccatgagg
     2521 tcaccttcca aaccgaattg ggccaattga tggacttaat cactgcacct gaagacaaag
     2581 tcgacttgag taagttctcc ctaaagaagc actccttcat agttactttc aagactgctt
     2641 actattcttt ctacttgcct gtcgcattgg ccatgtacgt tgccggtatc acggatgaaa
     2701 aggatttgaa acaagccaga gatgtcttga ttccattggg tgaatacttc caaattcaag
     2761 atgactactt agactgcttc ggtaccccag aacagatcgg taagatcggt acagatatcc
     2821 aagataacaa atgttcttgg gtaatcaaca aggcattaga acttgcttcc gcagaacaaa
     2881 gaaagacttt agacgaaaat tacggtaaga aggactcagt cgcagaagcc aaatgcaaaa
     2941 agattttcaa tgacttgaaa attgaacagc tataccacga atatgaagag tctattgcca
     3001 aggatttgaa ggctaaaatt tctcaggtcg atgagtctcg tggcttcaaa gctgatgtct
     3061 taactgcgtt tttgaacaaa gtttacaaga gaagcaaaag atctgctggt ggaggaggta
     3121 ctggtggtgc tgagctcatg gctaccgcag caggtcctat cgcaactaac aactccccac
     3181 aaaactccaa cgcttacaga actccaatcg ctccttccgt accaattact cataaatggt
     3241 ctatagctga agatttgaca tgtatttcca atcctagtaa gcacaataac cctcaaactg
     3301 gttacagatc attttctgac gaattatacg ttaagtacga agaaaagttg gaagatgtta
     3361 gaaaagcatt aagagaagtt gaagaaaacc ctttggaagg tttagttatg atagacgctt
     3421 tgcaaagatt gggtatcgat taccatttca gaggtgaaat tggtgcattc ttgcaaaagc
     3481 aacaaatcat atcttcaact ccagatggtt accctgaaca tggtttgtac gaagtttcaa
     3541 cattgtttag attcttaaga caagaaggtc acaatgttac cgctgacgtc tttaataact
     3601 tcaaggataa ggaaggtaga ttcagatcag aattgtcaac agatattaga ggtttgatgt
     3661 ccttatacga agcaagtcaa ttgagaatag aaggtgaaga catcttagat caagctgctg
     3721 atttctccag tcaattgtta ggtagatgga caaaagatcc taatcatcac gaagccagat
     3781 tggtttctaa cactttaaca catccatacc acaagtcatt ggctaccttt atgggtcaaa
     3841 aattgtccta catgaactgc aagggtccaa actgggacgg tgtcgataat ttgcaagaat
     3901 tagctaagat ggatttgact atcgtacaaa gtatccatca aaaggaagta ttccaagttt
     3961 ctcaatggtg gaaggataca ggtttagcca atgaattgaa attggctaga aaccaaccat
     4021 tgaagtggta tatgtggcct atggccgctt taaccgatcc aagattcagt gaagaaagag
     4081 ttgaattaac taagcctatt tcttttatat acatcataga tgacatcttc gacgtctatg
     4141 gtaccattga agaattgacc ttgtttactg atgcagttaa tagatgggaa ttgtctgccg
     4201 tcgaacaatt accagactac atgaaagtat gtttcaaggc tttgtacgat gttaccaacg
     4261 aaatcgcata caaaatctat aaaaagcatg gtcaaaaccc tattgattcc ttgcaaaaga
     4321 cttgggctag tttgtgcaat gcatttttag ttgaagcaaa gtggttcgcc tctggtcact
     4381 tgccaaatgc agaagaatac ttaaagaacg gtatcatctc ttcaggtgtt catgttgtct
     4441 tggcccacat gtttttcttg ttaggtgacg gtattacaca agaatcagtt gatttggtag
     4501 atgactatcc aggtatttcc acaagtatcg caaccatttt gagattatct gatgacttgg
     4561 gttcagccaa agatgaagac caagatggtt atgatggttc ttacatcgaa tgttacatga
     4621 aggaacataa gggttccagt gtcgattcag ccagagaaga agtaataaga atgatctccg
     4681 aagcatggaa atgtttgaat aaggaatgct tatcaccaaa ccctttttct gaatcattca
     4741 gaataggttc cttgaatatg gctagaatga tccctatgat gtactcttac gatgacaacc
     4801 ataacttgcc aattttagaa gaacacatga aggcaatgat ctataacaca tctttataac
     4861 tcgagataga ttaatttaaa cagtatatgt acagttttat atatatatat atatatatat
     4921 acatatataa agaaacctgt gcgttttttg tattttcaaa tacatttagt tttggcgcca
     4981 cttctataaa aggtctcatg atattgttac tctagagatc ccaatacaac agatcacgtg
     5041 atcttttgta agatgaagtt gaagtgagtg ttgcaccgtg ccaatgcagg tggctattag
     5101 attaaatatg tgatttgttc tattaagttt cctgtataat taatggggag cgctgattct
     5161 cttttggtac gcttcccatc cagcatttct gtatctttca ccttcaacct taggatctct
     5221 acccttggcg aaaagtcctc tgccaacaat gatgatatct gatccaccac ttacaacttc
     5281 gtcgacggtt ctgtactgct gacccaatgc atcgcctttg tcgtctaaac ctacacctgg
     5341 ggtcatgatt agccaatcaa acccttcttc tcttcctccc atatcgttct gagcaatgaa
     5401 cccaataacg aaatctttat cactctttgc aatatcaacg gtacccttag tatattcacc
     5461 gtgtgctaga gaacccttgg aagacaattc agcaagcatc aataatcccc ttggttcttt
     5521 ggtgacctct tgcgcacctt gtttcaagcc agcaacaata ccagcaccag taaccccgtg
     5581 ggcgttggtg atatcagacc attctgcgat acggtaaacg cccgatgtat attgtaattt
     5641 gactgtgtta ccgatatcgg cgaattttct gtcctcaaat atcaagaact tgtatttctc
     5701 tgccaatgct ttcaatggaa cgacagtacc ctcataactg aaatcatcca agatatcaac
     5761 gtgtgttttc aaaaggcaaa tgtatggacc caacgtttca acaagtttca atagctcatc
     5821 agtcgaacga acgtcaagag aagcacacaa attggtcttc ttttcatcca ttaaacgtaa
     5881 aagtttcgat gcaaccggac ttgcatgagt ctcagctcta ctggtatatg attttgtgga
     5941 catgttttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     6001 gcaatttcat gtttcttcaa caccacatat gcgtatatat accaatctaa gtctgtgctc
     6061 cttccttcgt tcttccttct gctcggagat taccgaatca aaaaaattta aatgaattca
     6121 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6181 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6241 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     6301 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     6361 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6421 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     6481 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     6541 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc
     8761 atttaaatca ttacgaccga gattcccggg taataactga tataattaaa ttgaagctct
     8821 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     8881 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     8941 atcccttccc tttgcaaata gtcctactag
//
