LOCUS       pAAV\TRE-Tight\CheRiff-mKate2        6178 bp    DNA     circular UNA 27-JAN-2022
ACCESSION   ChR(H134R)-2A-tDimer in pAAV (Ingrid)
SOURCE      Cloning vector pCI (unknown).
  ORGANISM  Cloning vector pCI
            Cloning vector pCI other sequences; artificial sequences; vectors.
COMMENT     CaMKIIa Promoter was cloned via MluI-EcoRI restriction sites
            Promoter was amplified with pcr by Daniel Udwari using template pAAV CaMKIIa eNpHR3.0 EYFP
            cloned into pAAV [Syn] ChR2 E123T-T159C 2A tDimer by Iris Ohmert
            Sequenced (with gaps) 17-12, Clone #61 Map corrected 05-12-2012
            Function tested in organotypic hippocampal cultures by Simon Wiegert
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14237985|
COMMENT     VNTDBDATE|-14237985|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV TRE-Tight CheRiff-mKate2|
COMMENT     VNTAUTHORNAME|Iris Ohmert|
COMMENT     VNTAUTHOREML|iohmert@zmnh.uni-hamburg.de|
COMMENT     VNTOAUTHORNAME|ISP|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        (CStringList "Acc65I" "AfeI" "AgeI" "ApaI" "AscI" "BamHI" "BclI" "BglI" 
COMMENT         "BglII" "BoxI" "BshTI" "Bsp1407I" "Bsu15I" "Cfr9I" "ClaI" "Eco147I" 
COMMENT         "Eco32I" "EcoRI" "HindIII" "KpnI" "MluI" "NcoI" "NdeI" "NheI" "NotI" 
COMMENT         "PasI" "PstI" "SacI" "SalI" "SanDI" "SgsI" "SmaI" "SphI" "XbaI" "XhoI" 
COMMENT         "AflII" "BspTI" "PdmI" "XmnI" "BmtI" "BspOI" "BspEI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
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COMMENT             "GTTACTTTTGAAGATGTTGCTGTTCTTTTTACTCGTGATGAATGGAAAAAACTTGATCTTTCTCAACGTTCTCTTTATCGTGAAGTTATGCTTGAAAATTATTCTAATCTT" 
COMMENT             "" 0 (CStringList) 0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 
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COMMENT       (CMolPar 1 0 0 0 0 1 6178 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT       (CObList #1=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AfeI" "agcgct" 4 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 505 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2723 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "BclI" "tgatca" 2 0 2 642 0 1401 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 8 596 0 872 0 1854 0 
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COMMENT        #10=(CRSite (CStringList) "BoxI" "gacnnnngtc" 6 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "BshTI" "accggt" 2 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "Bsp1407I" "tgtaca" 2 0 1 1428 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "Bsu15I" "atcgat" 3 0 2 2097 0 2692 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "Cfr9I" "cccggg" 2 0 4 3378 0 3389 0 6070 0 
COMMENT             6081 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 2097 0 2692 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "Eco147I" "aggcct" 4 0 1 278 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "Eco32I" "gatatc" 4 0 2 428 0 1210 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 324 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2090 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 2 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 719 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 1386 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 335 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 3 1290 0 3286 0 6173 0 0 
COMMENT             "") #26=(CRSite (CStringList) "PasI" "cccwggg" 3 0 1 1609 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1722 0 3431 0 6036 0 0 
COMMENT             "") 
COMMENT        #28=(CRSite (CStringList) "SacI" "gagctc" 6 0 3 295 0 352 0 1229 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2700 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "SanDI" "gggwccc" 3 0 1 1358 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "SgsI" "ggcgcgcc" 3 0 1 2723 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "SmaI" "cccggg" 4 0 4 3380 0 3391 0 6072 0 
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COMMENT        #33=(CRSite (CStringList) "SphI" "gcatgc" 6 0 2 1081 0 3093 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2761 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 2706 0 2755 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "AflII" "cttaag" 2 0 0 0 "") 
COMMENT        #37=(CRSite (CStringList) "BspTI" "cttaag" 2 0 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "PdmI" "gaannnnttc" 6 0 2 388 0 4537 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 388 0 4537 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "BmtI" "gctagc" 6 0 1 339 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "BspOI" "gctagc" 6 0 1 339 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "BspEI" "tccgga" 2 0 0 0 "")) 
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COMMENT             (CStringList) 1 1 1 1 "2929..3524") 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "3599..4076") 
COMMENT        #45=(CFSignal (CObList) "right ITR" 87 0 0 3292 3431 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4117..4256") 
COMMENT        #46=(CFSignal (CObList) "f1 origin" 33 0 0 3524 3829 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4349..4654") 
COMMENT        #47=(CFSignal (CObList) "pUC ori" 33 0 0 5357 6023 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6182..6848") 
COMMENT        #48=(CFSignal (CObList) "left ITR" 35 0 0 6030 6169 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6855..6994") 
COMMENT        #49=(CFSignal (CObList) "Linker" 21 0 0 1363 1383 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #50=(CFSignal (CObList) "mKate2" 21 0 0 1387 2082 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT             (CStringList) 1 1 1 1 "5174..6027") 
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COMMENT             (CStringList) 1 1 1 1 "")) 
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COMMENT                          "Currently local object. Original author: ISP" 1) 
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COMMENT                          "Created: 01/27/22 10:00" 1) 
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COMMENT                          "length: 6178 bp" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 6-JAN-2022" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/ChR(H134R)-2A-tDimer\">ChR(H134R)-2A-tDimer</a>; <a href=\"ncbilink:dnarna/in\">in</a>; <a href=\"ncbilink:dnarna/pAAV\">pAAV</a>; <a href=\"ncbilink:dnarna/(Ingrid)\">(Ingrid)</a>" 
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COMMENT                          "Sequence Source: Cloning vector pCI (unknown)." 1) 
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COMMENT                          "Organism(s): <a href=\"ncbilink:taxonomy/Cloning+vector+pCI\">Cloning vector pCI(Cloning vector pCI)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Cloning vector pCI other sequences; artificial sequences; vectors" 
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COMMENT                      #142=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 114407296 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" 
COMMENT                            "CCTAGG") 
COMMENT                      #143=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407168 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BclI: 2 sites") 1 5 "TGATCA" 
COMMENT                            "ACTAGT") 
COMMENT                      #144=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114406496 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BglI: 8 sites") 7 4 "GCCNNNNNGGC" 
COMMENT                            "CGGNNNNNCCG") 
COMMENT                      #145=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407392 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #146=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #147=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 660 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #148=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408864 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #149=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #40# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #150=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 339 " 1))))) "BmtI: 1 site") 5 
COMMENT                            1 "GCTAGC" "CGATCG") 
COMMENT                      #151=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114406784 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BoxI: 0 sites") 5 5 "GACNNNNGTC" 
COMMENT                            "CTGNNNNCAG") 
COMMENT                      #152=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114406688 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BshTI: 0 sites") 1 5 "ACCGGT" 
COMMENT                            "TGGCCA") 
COMMENT                      #153=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114820536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #154=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #12# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #155=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #12# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1428 " 1))))) 
COMMENT                             "Bsp1407I: 1 site") 1 5 "TGTACA" "ACATGT") 
COMMENT                      #156=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408768 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BspEI: 0 sites") 1 5 "TCCGGA" 
COMMENT                            "AGGCCT") 
COMMENT                      #157=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408736 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #158=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #159=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 339 " 1))))) "BspOI: 1 site") 
COMMENT                            5 1 "GCTAGC" "CGATCG") 
COMMENT                      #160=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114409536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BspTI: 0 sites") 1 5 "CTTAAG" 
COMMENT                            "GAATTC") 
COMMENT                      #161=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114406880 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "Bsu15I: 2 sites") 2 4 "ATCGAT" 
COMMENT                            "TAGCTA") 
COMMENT                      #162=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408032 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "Cfr9I: 4 sites") 1 5 "CCCGGG" 
COMMENT                            "GGGCCC") 
COMMENT                      #163=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408512 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ClaI: 2 sites") 2 4 "ATCGAT" 
COMMENT                            "TAGCTA") 
COMMENT                      #164=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408416 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #165=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #166=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 278 " 1))))) "Eco147I: 1 site") 
COMMENT                            3 3 "AGGCCT" "TCCGGA") 
COMMENT                      #167=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407680 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "Eco32I: 2 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
COMMENT                      #168=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408064 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #169=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #170=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 324 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #171=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408352 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #172=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #19# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #173=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #19# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2090 " 1))))) 
COMMENT                             "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #174=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                            "CCATGG") 
COMMENT                      #175=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408096 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #176=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #21# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #177=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #21# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2 " 1))))) "MluI: 1 site") 1 5 
COMMENT                            "ACGCGT" "TGCGCA") 
COMMENT                      #178=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407712 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #179=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #22# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #180=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 719 " 1))))) "NcoI: 1 site") 1 
COMMENT                            5 "CCATGG" "GGTACC") 
COMMENT                      #181=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407648 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #182=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #23# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #183=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1386 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
COMMENT                      #184=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407776 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #185=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #24# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #186=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #24# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 335 " 1))))) "NheI: 1 site") 1 
COMMENT                            5 "GCTAGC" "CGATCG") 
COMMENT                      #187=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407872 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 3 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #188=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408160 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #189=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #26# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #190=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #26# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1609 " 1))))) "PasI: 1 site") 
COMMENT                            2 5 "CCCWGGG" "GGGWCCC") 
COMMENT                      #191=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408576 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PdmI: 2 sites") 5 5 "GAANNNNTTC" 
COMMENT                            "CTTNNNNAAG") 
COMMENT                      #192=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114407968 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PstI: 3 sites") 5 1 "CTGCAG" 
COMMENT                            "GACGTC") 
COMMENT                      #193=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408192 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacI: 3 sites") 5 1 "GAGCTC" 
COMMENT                            "CTCGAG") 
COMMENT                      #194=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114409312 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #195=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #29# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #196=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2700 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #197=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408832 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #198=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #30# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #199=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1358 " 1))))) "SanDI: 1 site") 
COMMENT                            2 5 "GGGWCCC" "CCCWGGG") 
COMMENT                      #200=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408896 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #201=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #31# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #202=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #31# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2723 " 1))))) "SgsI: 1 site") 
COMMENT                            2 6 "GGCGCGCC" "CCGCGCGG") 
COMMENT                      #203=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114409216 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SmaI: 4 sites") 3 3 "CCCGGG" 
COMMENT                            "GGGCCC") 
COMMENT                      #204=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408800 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SphI: 2 sites") 5 1 "GCATGC" 
COMMENT                            "CGTACG") 
COMMENT                      #205=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114409408 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #206=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #34# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #207=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #34# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2761 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
COMMENT                      #208=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114409440 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XhoI: 2 sites") 1 5 "CTCGAG" 
COMMENT                            "GAGCTC") 
COMMENT                      #209=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 114408672 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XmnI: 2 sites") 5 5 "GAANNNNTTC" 
COMMENT                            "CTTNNNNAAG"))) 
COMMENT                    "Restriction/Methylation Map:  (no more than 1 site)") 
COMMENT              #210=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 14 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #211=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                            1) 
COMMENT                      #212=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                            "is calculated as an AVERAGE similarity" 1) 
COMMENT                      #213=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 112591424 (9 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #214=(CGroupPar 
COMMENT                                    (CParagraph 0 (11 #55# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #215=(COlyLinePar 
COMMENT                                           (CLinePar 
COMMENT                                            (CParagraph 0 
COMMENT                                             (17 
COMMENT                                              #216=(COligo 
COMMENT                                                    "KRAB-A Repressor domain" 
COMMENT                                                    "GTTACTTTTGAAGATGTTGCTGTTCTTTTTACTCGTGATGAATGGAAAAAACTTGATCTTTCTCAACGTTCTCTTTATCGTGAAGTTATGCTTGAAAATTATTCTAATCTT" 
COMMENT                                                    "" 0 (CStringList) 0) 0) 1 2 
COMMENT                                             2 0 181) 
COMMENT                                            " GTTACTTTTGAAGATGTTGCTGTTCTTTTTACTCGTGATGAATGGAAAAAACTTGATCTTTCTCAACGTTCTCTTTATCGTGAAGTTATGCTTGAAAATTATTCTAATCTT" 
COMMENT                                            1)))))) 
COMMENT                            "KRAB-A Repressor domain (0 sites)"))) 
COMMENT                    "Motifs (both strands)")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #217=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #218=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #219=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #220=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #221=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #222=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #223=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #224=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #225=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #226=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #227=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #228=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #229=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #230=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #231=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #232=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #233=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #234=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #235=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #236=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #237=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #238=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #239=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #240=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #241=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #242=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #243=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #244=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #245=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #246=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #247=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #248=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #249=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #250=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #251=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #252=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #253=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #254=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #255=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #256=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 87.75 87.75 
COMMENT         0.1 -6178) 1 1 1 1 1 
COMMENT        (mapper: 16.4379 -19.6473 40.0378 40.0378 0.01 10 -1 6178 6178 1 0 0) 
COMMENT        #257=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -666 100) 
COMMENT              (CObjectList 
COMMENT               #258=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -945 100) 
COMMENT                     (CObjectList 
COMMENT                      #259=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #223# 807411744 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.2752 6.2732 1 0.0214037) 0.101541) 
COMMENT                      #260=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #220# -137362 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 23 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pAAV TRE-Tight CheRiff-mKate2" 
COMMENT                            "@N" 1 0 0.871165 0 -4.55956 19.032 1.34873 Nil) 
COMMENT                      #261=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #219# -68661 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 15 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "6178 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -5.82624 2.67247 0.949103 Nil)) (CObjectList)) 
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FEATURES             Location/Qualifiers
     misc_feature    2916..7497
                     /vntifkey="21"
                     /label=pAAV-syn_ChRtDimer
                     /note="Region on donor sequence (pAAV-syn_ChRtDimer): 2844..479"
     misc_feature    2097..2692
                     /vntifkey="21"
                     /label=WRE
                     /note="Oriinal location: 2863..3458"
     polyA_signal    2774..3251
                     /vntifkey="25"
                     /label=hGH\polyA\Signal
                     /note="Oriinal location: 3533..4010"
     misc_signal     3292..3431
                     /vntifkey="87"
                     /label=right\ITR
                     /note="Oriinal location: 4051..4190"
     rep_origin      3524..3829
                     /vntifkey="33"
                     /label=f1\origin
                     /note="Oriinal location: 4283..4588"
     misc_structure  4349..5202
                     /vntifkey="88"
                     /label=AmpR
                     /note="Oriinal location: 5108..5961"
     rep_origin      5357..6023
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="Oriinal location: 6116..6782"
     repeat_unit     6030..6169
                     /vntifkey="35"
                     /label=left\ITR
                     /note="Oriinal location: 6789..6928"
     misc_feature    1363..1383
                     /vntifkey="21"
                     /label=Linker
     misc_feature    1387..2082
                     /vntifkey="21"
                     /label=mKate2
     misc_feature    7..252
                     /vntifkey="21"
                     /label=TRE
     misc_feature    7..318
                     /vntifkey="21"
                     /label=pTight
     CDS             340..1356
                     /vntifkey="4"
                     /label=Cheriff
BASE COUNT     1371 a      1654 c      1639 g      1514 t 
ORIGIN
        1 acgcgtttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc 
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg 
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata 
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta 
      301 gtgaaccgtc agatcgcctg gagaattcaa gctgctagca tgggcggagc tcctgctcca 
      361 gacgctcaca gcgccccacc tggaaacgat tctgccggag gcagtgagta ccatgcccca 
      421 gctggatatc aagtgaatcc accctaccac cccgtgcatg ggtatgagga acagtgcagc 
      481 tccatctaca tctactatgg ggccctgtgg gagcaggaaa cagctagggg cttccagtgg 
      541 tttgccgtgt tcctgtctgc cctgtttctg gctttctacg gctggcacgc ctataaggcc 
      601 agcgtgggat gggaggaagt gtacgtgtgc tccgtggagc tgatcaaagt gattctggag 
      661 atctatttcg agttcaccag tcctgctatg ctgttcctgt acggagggaa cattacccca 
      721 tggctgagat atgccgaatg gctgctgaca tgtcccgtga tcctgattca tctgtctaac 
      781 atcaccggcc tgagtgaggc atacaataag cggacaatgg ctctgctggt gtccgacctg 
      841 ggaactattt gcatgggagt gacagccgct ctggccactg ggtgggtgaa gtggctgttt 
      901 tactgtatcg gcctggtgta tggaacccag acattctaca acgctggaat catctacgtg 
      961 gagtcttact atatcatgcc tgccggcggc tgtaagaaac tggtgctggc catgactgcc 
     1021 gtgtactatt ctagttggct gatgtttccc ggcctgttca tctttgggcc tgaaggcatg 
     1081 cacaccctga gcgtggctgg gtccactatt ggccatacca tcgccgacct gctgtccaag 
     1141 aatatttggg gactgctggg gcacttcctg cggatcaaaa ttcacgagca tatcattatg 
     1201 tacggcgata tcaggagacc agtgagctcc cagtttctgg gacgcaaggt ggacgtgctg 
     1261 gccttcgtga cagaggaaga taaagtggcg gccgccaaga gcaggatcac cagcgagggc 
     1321 gagtacatcc ccctggacca gatcgacatc aacgtgggac ccgtggctgc tcctgtggtg 
     1381 gctcatatgg tgtccgagct gatcaaagaa aacatgcaca tgaagctgta catggaaggc 
     1441 accgtgaaca accaccactt caagtgcacc agcgagggcg agggcaagcc ttacgagggc 
     1501 acccagacca tgagaatcaa ggccgtggaa ggcggccctc tgcccttcgc ctttgacatc 
     1561 ctggccacca gctttatgta cggcagcaag accttcatca accacaccca gggcatcccc 
     1621 gatttcttca agcagagctt ccccgagggc ttcacctggg agagagtgac cacatacgag 
     1681 gacggcggag tgctgaccgc cacccaggat acaagcctgc aggacggctg cctgatctac 
     1741 aacgtgaaga tcagaggcgt gaacttcccc agcaacggcc ctgtgatgca gaagaaaaca 
     1801 ctgggctggg aggccagcac cgagacactg tatcctgctg acggcggcct ggaaggcaga 
     1861 gccgatatgg ccctgaaact cgtgggcgga ggccacctga tctgtaacct gaaaaccacc 
     1921 tacaggtcca agaagcccgc caagaacctg aagatgcccg gcgtgtacta cgtggacaga 
     1981 aggctggaaa gaatcaaaga ggccgacaaa gaaacctacg tggaacagca cgaggtggcc 
     2041 gtggctagat actgcgacct gcctagcaag ctgggacaca gatgacaaaa gcttatcgat 
     2101 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 
     2161 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 
     2221 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 
     2281 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 
     2341 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 
     2401 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 
     2461 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 
     2521 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 
     2581 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 
     2641 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt 
     2701 cgacctcgag acgcgatcgc ggcgcgccat tcgatggcct ctctggcctc gacctcgagt 
     2761 ctagagatga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg 
     2821 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg 
     2881 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg 
     2941 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca 
     3001 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct 
     3061 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt 
     3121 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag 
     3181 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc 
     3241 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc 
     3301 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 
     3361 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 
     3421 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 
     3481 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 
     3541 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     3601 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     3661 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     3721 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     3781 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     3841 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     3901 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 
     3961 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 
     4021 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 
     4081 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 
     4141 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 
     4201 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     4261 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     4321 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
     4381 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
     4441 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
     4501 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
     4561 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
     4621 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
     4681 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
     4741 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
     4801 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
     4861 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
     4921 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
     4981 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
     5041 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
     5101 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
     5161 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     5221 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     5281 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     5341 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     5401 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     5461 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     5521 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     5581 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     5641 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     5701 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     5761 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     5821 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     5881 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     5941 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     6001 ggccttttgc tggccttttg ctcacatgtc ctgcaggcag ctgcgcgctc gctcgctcac 
     6061 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag 
     6121 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgc 
//
