LOCUS       IAA17_3xHA_ARF16        9745 bp    DNA        circular     27-JAN-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(6969..6988)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6807..6812
                     /locus_tag="MluI Site"
                     /label="MluI Site"
                     /ApEinfo_label="MluI Site"
                     /ApEinfo_fwdcolor="#4364ff"
                     /ApEinfo_revcolor="#4364ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3502..3531)
                     /locus_tag="I-Sce1 18 n Restriction site"
                     /label="I-Sce1 18 n Restriction site"
                     /ApEinfo_label="I-Sce1 18 n Restriction site"
                     /ApEinfo_fwdcolor="#eeff73"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6813..6818
                     /locus_tag="BbeI Site"
                     /label="BbeI Site"
                     /ApEinfo_label="BbeI Site"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5055..6803
                     /locus_tag="TIR1 MYC ORF"
                     /label="TIR1 MYC ORF"
                     /ApEinfo_label="TIR1 MYC ORF"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3532..3621
                     /locus_tag="N-Terminus of Sir4"
                     /label="N-Terminus of Sir4"
                     /ApEinfo_label="N-Terminus of Sir4"
                     /ApEinfo_fwdcolor="#d84bfe"
                     /ApEinfo_revcolor="#d84bfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3922..4317
                     /locus_tag="ADH1 Promoter"
                     /label="ADH1 Promoter"
                     /ApEinfo_label="ADH1 Promoter"
                     /ApEinfo_fwdcolor="#5f5eff"
                     /ApEinfo_revcolor="#5f5eff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2478..2495
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3483..3501
                     /locus_tag="S2-Site"
                     /label="S2-Site"
                     /ApEinfo_label="S2-Site"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7006..7026)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2614..2631
                     /locus_tag="S1/S3-Site"
                     /label="S1/S3-Site"
                     /ApEinfo_label="S1/S3-Site"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2504..2524
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3502..3531)
                     /locus_tag="I-SceI site"
                     /label="I-SceI site"
                     /ApEinfo_label="I-SceI site"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5019..5039
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#ffc976"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(7394..8076)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3436..3447
                     /locus_tag="Annealing Sequence for Reverse Primer"
                     /label="Annealing Sequence for Reverse Primer"
                     /ApEinfo_label="Annealing Sequence for Reverse Primer"
                     /ApEinfo_fwdcolor="#be99ff"
                     /ApEinfo_revcolor="#be99ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4992..5018
                     /locus_tag="Linker from pGADT7"
                     /label="Linker from pGADT7"
                     /ApEinfo_label="Linker from pGADT7"
                     /ApEinfo_fwdcolor="#ff323c"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2592..2613)
                     /locus_tag="I-SceI site(1)"
                     /label="I-SceI site(1)"
                     /ApEinfo_label="I-SceI site"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2011..2317)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5040..5054
                     /locus_tag="Linker from pGADT7(1)"
                     /label="Linker from pGADT7(1)"
                     /ApEinfo_label="Linker from pGADT7"
                     /ApEinfo_fwdcolor="#ff141d"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2614..2658
                     /locus_tag="L3 Linker Sequence"
                     /label="L3 Linker Sequence"
                     /ApEinfo_label="L3 Linker Sequence"
                     /ApEinfo_fwdcolor="#fb244b"
                     /ApEinfo_revcolor="#fb244b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1879..2334)
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        3622..4304
                     /locus_tag="ADH1 promoter"
                     /label="ADH1 promoter"
                     /ApEinfo_label="ADH1 promoter"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3430..3447
                     /locus_tag="Reverse Primer Annealing"
                     /label="Reverse Primer Annealing"
                     /ApEinfo_label="Reverse Primer Annealing"
                     /ApEinfo_fwdcolor="#ff8171"
                     /ApEinfo_revcolor="#ff8171"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3457..3501
                     /locus_tag="L4 Linker Sequence"
                     /label="L4 Linker Sequence"
                     /ApEinfo_label="L4 Linker Sequence"
                     /ApEinfo_fwdcolor="#67ff4c"
                     /ApEinfo_revcolor="#67ff4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2339..2407)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(7032..7054)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3401..3425
                     /locus_tag="3HA_CONF_F"
                     /label="3HA_CONF_F"
                     /ApEinfo_label="3HA_CONF_F"
                     /ApEinfo_fwdcolor="#35ff29"
                     /ApEinfo_revcolor="#35ff29"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(8174..8833)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3355..3381
                     /locus_tag="HA epitope"
                     /label="HA epitope"
                     /ApEinfo_label="HA epitope"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6804..6806
                     /locus_tag="End of SIR4 ORF"
                     /label="End of SIR4 ORF"
                     /ApEinfo_label="End of SIR4 ORF"
                     /ApEinfo_fwdcolor="#fb3350"
                     /ApEinfo_revcolor="#fb3350"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3385..3411
                     /locus_tag="HA epitope(1)"
                     /label="HA epitope(1)"
                     /ApEinfo_label="HA epitope"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      9163..9673
                     /locus_tag="CEN-ARS pRS"
                     /label="CEN-ARS pRS"
                     /ApEinfo_label="CEN-ARS pRS"
                     /ApEinfo_fwdcolor="#ff6fcf"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3418..3444
                     /locus_tag="HA epitope(2)"
                     /label="HA epitope(2)"
                     /ApEinfo_label="HA epitope"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(440..1774)
                     /locus_tag="Met15 ORF"
                     /label="Met15 ORF"
                     /ApEinfo_label="Met15 ORF"
                     /ApEinfo_fwdcolor="#6851ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(157..227)
                     /locus_tag="Gap Repair Primer"
                     /label="Gap Repair Primer"
                     /ApEinfo_label="Gap Repair Primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1808..1878)
                     /locus_tag="Other Gap Repair Primer"
                     /label="Other Gap Repair Primer"
                     /ApEinfo_label="Other Gap Repair Primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6906..6935)
                     /locus_tag="I-SceI site(2)"
                     /label="I-SceI site(2)"
                     /ApEinfo_label="I-SceI site"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6906..6935)
                     /locus_tag="I-Sce1 18 n Restriction site(1)"
                     /label="I-Sce1 18 n Restriction site(1)"
                     /ApEinfo_label="I-Sce1 18 n Restriction site"
                     /ApEinfo_fwdcolor="#eeff73"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6863..6905
                     /locus_tag="SIR4 3' Homology Flank"
                     /label="SIR4 3' Homology Flank"
                     /ApEinfo_label="SIR4 3' Homology Flank"
                     /ApEinfo_fwdcolor="#7b9efe"
                     /ApEinfo_revcolor="#7b9efe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2391..2411
                     /locus_tag="Sir4_tag_SEQ_F"
                     /label="Sir4_tag_SEQ_F"
                     /ApEinfo_label="Sir4_tag_SEQ_F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6777..6803
                     /locus_tag="I bet this is a linker on the end of TIR1"
                     /label="I bet this is a linker on the end of TIR1"
                     /ApEinfo_label="I bet this is a linker on the end of TIR1"
                     /ApEinfo_fwdcolor="#acff00"
                     /ApEinfo_revcolor="#acff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6819..6862
                     /locus_tag="End of SIR4 ORF(1)"
                     /label="End of SIR4 ORF(1)"
                     /ApEinfo_label="End of SIR4 ORF"
                     /ApEinfo_fwdcolor="#fb3350"
                     /ApEinfo_revcolor="#fb3350"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3789..3811
                     /locus_tag="AID_SEQ_R"
                     /label="AID_SEQ_R"
                     /ApEinfo_label="AID_SEQ_R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6601..6623
                     /locus_tag="TIR1_SEQ_F 897"
                     /label="TIR1_SEQ_F 897"
                     /ApEinfo_label="TIR1_SEQ_F 897"
                     /ApEinfo_fwdcolor="#ff2d1a"
                     /ApEinfo_revcolor="#ff2d1a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6771..6798
                     /locus_tag="TIR1_internal_F2 946"
                     /label="TIR1_internal_F2 946"
                     /ApEinfo_label="TIR1_internal_F2 946"
                     /ApEinfo_fwdcolor="#f6ff9c"
                     /ApEinfo_revcolor="#f6ff9c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4359..4889
                     /locus_tag="ARF16 PB1 Domain Codon Optimized"
                     /label="ARF16 PB1 Domain Codon Optimized"
                     /ApEinfo_label="ARF16 PB1 Domain Codon Optimized"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4890..4916
                     /locus_tag="HA Tag"
                     /label="HA Tag"
                     /ApEinfo_label="HA Tag"
                     /ApEinfo_fwdcolor="#42ff33"
                     /ApEinfo_revcolor="#42ff33"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4926..4982
                     /locus_tag="PTV P2A Cleavage Site"
                     /label="PTV P2A Cleavage Site"
                     /ApEinfo_label="PTV P2A Cleavage Site"
                     /ApEinfo_fwdcolor="#6c7eff"
                     /ApEinfo_revcolor="#6c7eff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4332..4352
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ff86fb"
                     /ApEinfo_revcolor="#ff86fb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2659..3339
                     /locus_tag="Codon-optimized IAA17 full length sequence"
                     /label="Codon-optimized IAA17 full length sequence"
                     /ApEinfo_label="Codon-optimized IAA17 full length
                     sequence"
                     /ApEinfo_fwdcolor="#ff261b"
                     /ApEinfo_revcolor="#ff261b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4228..4248
                     /locus_tag="218"
                     /label="218"
                     /ApEinfo_label="218"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3031..3058
                     /locus_tag="IAA17_SEQ_F"
                     /label="IAA17_SEQ_F"
                     /ApEinfo_label="IAA17_SEQ_F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3448..3450
                     /locus_tag="STOP CODON"
                     /label="STOP CODON"
                     /ApEinfo_label="STOP CODON"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCACGCTTT TCAATTCAAT TCATCACCAT CCTCATGAAA ACTGTGTAAC ATAATAACCG
      241 AAGTGTCGAA AAGGTGGCAC CTTGTCCAAT TGAACACGCT CGATGAAAAA AATAAGATAT
      301 ATATAAGGTT AAGTAAAGCG TCTGTTAGAA AGGAAGTTTT TCCTTTTTCT TGCTCTCTTG
      361 TCTTTTCATC TACTATTTCC TTCGTGTAAT ACAGGGTCGT CAGATACATA GATACAATTC
      421 TATTACCCCC ATCCATACAA TGCCATCTCA TTTCGATACT GTTCAACTAC ACGCCGGCCA
      481 AGAGAACCCT GGTGACAATG CTCACAGATC CAGAGCTGTA CCAATTTACG CCACCACTTC
      541 TTATGTTTTC GAAAACTCTA AGCATGGTTC GCAATTGTTT GGTCTAGAAG TTCCAGGTTA
      601 CGTCTATTCC CGTTTCCAAA ACCCAACCAG TAATGTTTTG GAAGAAAGAA TTGCTGCTTT
      661 AGAAGGTGGT GCTGCTGCTT TGGCTGTTTC CTCCGGTCAA GCCGCTCAAA CCCTTGCCAT
      721 CCAAGGTTTG GCACACACTG GTGACAACAT CGTTTCCACT TCTTACTTAT ACGGTGGTAC
      781 TTATAACCAG TTCAAAATCT CGTTCAAAAG ATTTGGTATC GAGGCTAGAT TTGTTGAAGG
      841 TGACAATCCA GAAGAATTCG AAAAGGTCTT TGATGAAAGA ACCAAGGCTG TTTATTTGGA
      901 AACCATTGGT AATCCAAAGT ACAATGTTCC GGATTTTGAA AAAATTGTTG CAATTGCTCA
      961 CAAACACGGT ATTCCAGTTG TCGTTGACAA CACATTTGGT GCCGGTGGTT ACTTCTGTCA
     1021 GCCAATTAAA TACGGTGCTG ATATTGTAAC ACATTCTGCT ACCAAATGGA TTGGTGGTCA
     1081 TGGTACTACT ATCGGTGGTA TTATTGTTGA CTCTGGTAAG TTCCCATGGA AGGACTACCC
     1141 AGAAAAGTTC CCTCAATTCT CTCAACCTGC CGAAGGATAT CACGGTACTA TCTACAATGA
     1201 AGCCTACGGT AACTTGGCAT ACATCGTTCA TGTTAGAACT GAACTATTAA GAGATTTGGG
     1261 TCCATTGATG AACCCATTTG CCTCTTTCTT GCTACTACAA GGTGTTGAAA CATTATCTTT
     1321 GAGAGCTGAA AGACACGGTG AAAATGCATT GAAGTTAGCC AAATGGTTAG AACAATCCCC
     1381 ATACGTATCT TGGGTTTCAT ACCCTGGTTT AGCATCTCAT TCTCATCATG AAAATGCTAA
     1441 GAAGTATCTA TCTAACGGTT TCGGTGGTGT CTTATCTTTC GGTGTAAAAG ACTTACCAAA
     1501 TGCCGACAAG GAAACTGACC CATTCAAACT TTCTGGTGCT CAAGTTGTTG ACAATTTAAA
     1561 GCTTGCCTCT AACTTGGCCA ATGTTGGTGA TGCCAAGACC TTAGTCATTG CTCCATACTT
     1621 CACTACCCAC AAACAATTAA ATGACAAAGA AAAGTTGGCA TCTGGTGTTA CCAAGGACTT
     1681 AATTCGTGTC TCTGTTGGTA TCGAATTTAT TGATGACATT ATTGCAGACT TCCAGCAATC
     1741 TTTTGAAACT GTTTTCGCTG GCCAAAAACC ATGAGTGTGC GTAATGAGTT GTAAAATTAT
     1801 GTATAAACCT ACTTTCTCTC ACAAGTACTG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
     1861 AGAAAATACC GCATCAGGaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc
     2341 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2401 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2461 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     2521 agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
     2581 aattcctgca gCTATATTAC CCTGTTATCC CTACGTACGC TGCAGGTCGA Cggtggcggt
     2641 tctggcggtg gcggatccGG CTCTGTTGAG TTAAACCTGC GTGAGACCGA GCTATGTCTT
     2701 GGTCTACCCG GTGGTGACAC TGTAGCACCA GTGACAGGCA ACAAAAGGGG CTTCAGCGAG
     2761 ACTGTAGATC TGAAGCTAAA CTTAAACAAC GAACCAGCTA ATAAGGAAGG TAGTACAACA
     2821 CATGATGTGG TTACGTTTGA CAGCAAGGAG AAGAGTGCCT GTCCCAAAGA TCCGGCAAAG
     2881 CCCCCGGCGA AGGCCCAGGT TGTAGGTTGG CCTCCGGTAA GGTCCTATCG TAAAAACGTA
     2941 ATGGTTAGTT GTCAGAAGTC CAGCGGAGGA CCTGAAGCAG CGGCGTTCGT CAAAGTATCT
     3001 ATGGATGGCG CCCCATACTT GAGAAAGATA GACCTGCGTA TGTATAAGTC TTACGACGAG
     3061 TTATCAAATG CGCTATCTAA CATGTTCAGC AGTTTCACCA TGGGCAAACA TGGTGGTGAG
     3121 GAGGGAATGA TCGACTTCAT GAACGAGAGA AAACTTATGG ACCTGGTGAA TAGCTGGGAT
     3181 TATGTCCCTA GCTACGAGGA TAAAGACGGG GATTGGATGC TTGTGGGGGA TGTTCCGTGG
     3241 CCGATGTTCG TCGATACGTG TAAGCGTCTG CGTCTAATGA AGGGCTCCGA TGCTATTGGT
     3301 TTAGCTCCGC GTGCTATGGA AAAGTGTAAG TCCAGAGCTg tatcacccgg ggcataccca
     3361 tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat
     3421 ccatatgacg ttccagatta cgctgcttga cttaagagtt cttctttgag atatcgattg
     3481 aacgagctcg aattcATCGA TCTATATTAC CCTGTTATCC CTAGCGTAAC TATGCCAAAT
     3541 GACAATAAGA CACCCAATAG GTCCAGCACT CCCAAGTTTA CTAAAAAACC AGTAACCCCG
     3601 AATGATAAAA TTCCTGAAAG AcGGGTGTAC AATATGGACT TCCTCTTTTC TGGCAACCAA
     3661 ACCCATACAT CGGGATTCCT ATAATACCTT CGTTGGTCTC CCTAACATGT AGGTGGCGGA
     3721 GGGGAGATAT ACAATAGAAC AGATACCAGA CAAGACATAA TGGGCTAAAC AAGACTACAC
     3781 CAATTACACT GCCTCATTGA TGGTGGTACA TAACGAACTA ATACTGTAGC CCTAGACTTG
     3841 ATAGCCATCA TCATATCGAA GTTTCACTAC CCTTTTTCCA TTTGCCATCT ATTGAAGTAA
     3901 TAATAGGCGC ATGCAACTTC TTTTCTTTTT TTTTCTTTTC TCTCTCCCCC GTTGTTGTCT
     3961 CACCATATCC GCAATGACAA AAAAATGATG GAAGACACTA AAGGAAAAAA TTAACGACAA
     4021 AGACAGCACC AACAGATGTC GTTGTTCCAG AGCTGATGAG GGGTATCTCG AAGCACACGA
     4081 AACTTTTTCC TTCCTTCATT CACGCACACT ACTCTCTAAT GAGCAACGGT ATACGGCCTT
     4141 CCTTCCAGTT ACTTGAATTT GAAATAAAAA AAAGTTTGCT GTCTTGCTAT CAAGTATAAA
     4201 TAGACCTGCA ATTATTAATC TTTTGTTTCC TCGTCATTGT TCTCGTTCCC TTTCTTCCTT
     4261 GTTTCTTTTT CTGCACAATA TTTCAAGCTA TACCAAGCAT ACAATCAACT ATCTCATATA
     4321 CAactagaAT GCCCAAGAAG AAACGTAAGG TGGGTTCCAC CCTTACGAGC GGTCAACGTG
     4381 ACCACAGAGA CGTTGAAAAT GAGTTATCCA GTGCAGCTTT TAGCAGTCAA AGTTTCGGGG
     4441 TTCCAGATAT GTCATTCAAA CCGGGGTGCT CCAGTGACGT CGCAGTCACT GATGCGGGTA
     4501 TGCCTAGCCA AGGGTTATGG AACAACCAGA CGCAAAGGAT GCGTACCTTC ACTAAGGTCC
     4561 AGAAAAGGGG CTCTGTTGGG AGGAGTATCG ACATCACCAG GTATCGTGAT TACGATGAGC
     4621 TACGTCATGA TCTGGCATGT ATGTTCGGCA TACAGGGGCA GTTAGAAGAC CCTTATCGTA
     4681 TGGATTGGAA GTTAGTTTAT GTGGATCACG AGAACGATAT ACTGTTGGTG GGCGACGACC
     4741 CGTGGGAGGA ATTTGTAGGT TGTGTTAAGT CTATCAAGAT TCTATCTGCT GCAGAGGTGC
     4801 AGCAAATGAG CTTGGACGGG GATCTTGGTG GAGTTCCGCC CCAAACCCAG GCGTGCAGTG
     4861 CTTCAGACGA TGCGAACGCT TGGAGAGGCT ATCCCTACGA CGTGCCTGAT TATGCGGGTA
     4921 GCGGGGCCAC TAACTTCAGC TTGTTGAAGC AGGCTGGCGA TGTGGAGGAG AATCCAGGTC
     4981 CGGCGGCTAC CGATAAAGCG GAATTAATTC CCGAGCCTCC AAAAAAGAAG AGAAAGGTCG
     5041 AATTGGGTAC CGCCACGTAC TTCCCGGAGG AGGTGGTGGA GCACATCTTC AGCTTCCTGC
     5101 CGGCGCAGCG CGACCGCAAC ACGGTCTCGC TCGTCTGCAA GGTGTGGTAC GAGATCGAGA
     5161 GGCTGAGCCG CCGCGGCGTC TTCGTGGGCA ACTGCTACGC CGTGCGCGCC GGCCGCGTCG
     5221 CCGCGCGGTT CCCCAACGTG CGGGCGCTCA CGGTGAAGGG GAAGCCCCAC TTCGCCGACT
     5281 TCAACCTCGT GCCCCCCGAC TGGGGCGGCT ACGCGGGGCC GTGGATCGAG GCGGCCGCGA
     5341 GGGGATGCCA CGGCCTGGAG GAGCTCAGGA TGAAGCGGAT GGTGGTGTCC GACGAGAGCC
     5401 TCGAGCTGCT GGCTCGCTCG TTCCCGCGGT TCAGGGCTCT TGTTCTTATC AGCTGCGAGG
     5461 GGTTCAGCAC TGACGGGCTA GCCGCCGTCG CGAGCCATTG CAAGCTTCTG AGGGAGTTGG
     5521 ATTTGCAGGA AAATGAAGTG GAGGATCGAG GGCCTAGGTG GCTTTCCTGC TTCCCTGATT
     5581 CCTGCACATC ACTTGTCTCA TTGAATTTTG CCTGCATCAA AGGGGAGGTT AATGCTGGTT
     5641 CACTGGAGAG ACTTGTTAGC AGGTCCCCAA ACCTGCGGAG TTTGAGGCTG AATCGATCTG
     5701 TATCGGTAGA TACACTTGCA AAGATACTAC TGCGTACCCC TAACTTGGAG GATTTGGGGA
     5761 CAGGGAATTT GACAGATGAC TTCCAAACTG AGTCCTACTT TAAGCTTACC AGTGCTCTGG
     5821 AGAAATGCAA GATGTTGAGG AGTTTGTCTG GATTCTGGGA TGCTTCTCCT GTTTGCCTGT
     5881 CATTTATCTA CCCCCTGTGT GCTCAACTGA CAGGATTGAA CTTGAGCTAT GCCCCCACAC
     5941 TTGATGCTTC TGACCTTACA AAAATGATTA GCCGCTGTGT GAAGCTCCAA CGCCTTTGGG
     6001 TACTGGATTG TATCTCGGAC AAAGGCTTGC AAGTGGTGGC CTCCAGTTGC AAAGACTTGC
     6061 AAGAACTCAG GGTATTTCCA TCAGATTTCT ACGTAGCTGG TTATTCTGCA GTGACAGAGG
     6121 AGGGACTTGT TGCAGTATCC TTGGGCTGTC CAAAACTGAA CTCACTACTG TACTTCTGTC
     6181 ACCAAATGAC TAATGCTGCA CTAGTTACTG TCGCCAAGAA CTGTCCAAAT TTCACACGAT
     6241 TCAGACTTTG TATTCTTGAG CCAGGGAAGC CTGATGTTGT GACAAGCCAA CCATTAGATG
     6301 AAGGCTTTGG AGCTATTGTT CGTGAGTGCA AGGGATTACA ACGTTTGTCA ATATCTGGTC
     6361 TTCTCACAGA CAAAGTTTTC ATGTATATTG GGAAATATGC AAAACAACTT GAGATGCTTT
     6421 CTATAGCATT TGCTGGTGAC AGTGATAAGG GTATGATGCA TGTTATGAAT GGATGCAAGA
     6481 ATTTAAGGAA ACTGGAGATA AGAGATAGCC CGTTTGGTGA TGCTGCACTC TTGGGGAATT
     6541 TTGCTAGGTA CGAGACAATG CGATCCCTTT GGATGTCATC TTGCAATGTC ACGTTAAAGG
     6601 GGTGCCAAGT CCTTGCGTCA AAGATGCCGA TGCTCAATGT TGAGGTCATA AATGAGCGGG
     6661 ATGGTAGCAA TGAAATGGAG GAAAACCATG GAGATCTGCC TAAAGTGGAG AAATTATATG
     6721 TGTACCGCAC AACTGCTGGG GCGAGGGATG ATGCACCAAA TTTTGTTAAA ATCCTAgtcg
     6781 acTCCGGTTC TGCTGCTAGT GGTTGAacgc gtggcgccTG GAAAAAGATT TTCAAGTGAA
     6841 TAAGGAGATA AAACCGTATT GACTTTTTAT CATTCTACAA AAGACCAGTA ACGAAGTGTA
     6901 CCCTGCTATA TTACCCTGTT ATCCCTAGCG TAACTcggcc gccaccgcgg tggagctcca
     6961 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     7021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc ggaagcataa
     7081 agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg ttgcgctcac
     7141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg
     9181 ctataaaaat aattataatt taaatttttt aatataaata tataaattaa aaatagaaag
     9241 taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg
     9301 gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg
     9361 aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt
     9421 acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata
     9481 tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc
     9541 cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa
     9601 ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag
     9661 ttacaggcaa gcgatccgtc ctaagaaacc attattatca tgacattaac ctataaaaat
     9721 aggcgtatca cgaggccctt tcgtc   
//
