LOCUS       415_Gal_Cas9_Gal       11986 bp    DNA        circular     27-JAN-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3951..3970
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8897..8914)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3913..3933
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(8868..8888)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2863..3545
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      9075..9381
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      9058..9513
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             8985..9053
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1614..2804)
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    3885..3907
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(2363..2804)
                     /gene="S. cerevisiae GAL1"
                     /note="inducible promoter, regulated by Gal4"
                     /locus_tag="GAL1 promoter"
                     /label="GAL1 promoter"
                     /ApEinfo_label="GAL1 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             913..1572
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(2687..2804)
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
                     /locus_tag="UAS"
                     /label="UAS"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(73..583)
                     /locus_tag="CEN-ARS pRS"
                     /label="CEN-ARS pRS"
                     /ApEinfo_label="CEN-ARS pRS"
                     /ApEinfo_fwdcolor="#ff6fcf"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2317..2337)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4451..8590
                     /locus_tag="Cas9 ORF"
                     /label="Cas9 ORF"
                     /ApEinfo_label="Cas9 ORF"
                     /ApEinfo_fwdcolor="#98ff68"
                     /ApEinfo_revcolor="#98ff68"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10218..10220
                     /locus_tag="Start ATG of LEU2 ORF"
                     /label="Start ATG of LEU2 ORF"
                     /ApEinfo_label="Start ATG of LEU2 ORF"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10218..11324
                     /locus_tag="LEU2 ORF"
                     /label="LEU2 ORF"
                     /ApEinfo_label="LEU2 ORF"
                     /ApEinfo_fwdcolor="#644efe"
                     /ApEinfo_revcolor="#644efe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    9574..10217
                     /locus_tag="LEU2 Promoter"
                     /label="LEU2 Promoter"
                     /ApEinfo_label="LEU2 Promoter"
                     /ApEinfo_fwdcolor="#fc63bf"
                     /ApEinfo_revcolor="#fc63bf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11325..11788
                     /locus_tag="LEU2 3'UTR"
                     /label="LEU2 3'UTR"
                     /ApEinfo_label="LEU2 3'UTR"
                     /ApEinfo_fwdcolor="#fda376"
                     /ApEinfo_revcolor="#fda376"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3988..3999
                     /locus_tag="start of gal promoter"
                     /label="start of gal promoter"
                     /ApEinfo_label="start of gal promoter"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3988..4406
                     /locus_tag="GAL1 Promoter"
                     /label="GAL1 Promoter"
                     /ApEinfo_label="GAL1 Promoter"
                     /ApEinfo_fwdcolor="#fb2526"
                     /ApEinfo_revcolor="#fb2526"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1612..2346
                     /locus_tag="ISCE1 ORF with NLS"
                     /label="ISCE1 ORF with NLS"
                     /ApEinfo_label="ISCE1 ORF with NLS"
                     /ApEinfo_fwdcolor="#f2ff91"
                     /ApEinfo_revcolor="#f2ff91"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1483..1508
                     /locus_tag="AMP_SEQ_F"
                     /label="AMP_SEQ_F"
                     /ApEinfo_label="AMP_SEQ_F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2881..2905
                     /locus_tag="ColE1_Origin_SEQ_R"
                     /label="ColE1_Origin_SEQ_R"
                     /ApEinfo_label="ColE1_Origin_SEQ_R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4655..4674
                     /locus_tag="CAS9_SEQ_R"
                     /label="CAS9_SEQ_R"
                     /ApEinfo_label="CAS9_SEQ_R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tttatttcag
     1621 gaaagtttcg gaggagatag tgttcggcag tttgtacatc atctgcggga tcaggtacgg
     1681 tttgatcagg ttgtagaaga tcaggtaaga catagaatcg atgtagatga tcggtttgtt
     1741 tttgttgatt tttacgtaac agttcagttg gaatttgtta cgcagaccct taaccaggta
     1801 ttctacttct tcgaaagtga aagactgggt gttcagtacg atcgatttgt tggtagagtt
     1861 tttgttgtaa tcccatttac caccatcatc catgaaccag tatgccagag acatcggggt
     1921 caggtagttt tcaaccaggt tgttcgggat ggtttttttg ttgttaacga tgaacaggtt
     1981 agccagtttg ttgaaagctt ggtgtttgaa agtctgggcg ccccaggtga ttaccaggtt
     2041 acccaggtgg ttaacacgtt cttttttgtg cggcggggac agtacccact gatcgtacag
     2101 cagacatacg tggtccatgt atgctttgtt tttccactcg aactgcatac agtaggtttt
     2161 accttcatca cgagaacgga tgtaagcatc acccaggatc agaccgatac ctgcttcgaa
     2221 ctgttcgatg ttcagttcga tcagctggga tttgtattct ttcagcagtt tagagttcgg
     2281 acccaggttc attacctggt tttttttgat gtttttaacc tttctcttct tttttggcat
     2341 atgcatggat ccggggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt
     2401 tttgttgata cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt
     2461 attagttaaa gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca
     2521 tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct
     2581 atggttgtta atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt
     2641 tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc
     2701 gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag
     2761 tcttccttcg gagggctgtc acccgctcgg cggcttctaa tccggattta aaacttcatt
     2821 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3061 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3601 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3721 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3781 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg
     3841 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat
     3901 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     3961 actaaaggga acaaaagctg gAGCTCGTGA CAGCCCTCCG AAGGAAGACT CTCCTCCGTG
     4021 CGTCCTCGTC TTCACCGGTC GCGTTCCTGA AACGCAGATG TGCCTCGCGC CGCACTGCTC
     4081 CGAACAATAA AGATTCTACA ATACTAGCTT TTATGGTTAT GAAGAGGAAA AATTGGCAGT
     4141 AACCTGGCCC CACAAACCTT CAAATGAACG AATCAAATTA ACAACCATAG GATGATAATG
     4201 CGATTAGTTT TTTAGCCTTA TTTCTGGGGT AATTAATCAG CGAAGCGATG ATTTTTGATC
     4261 TATTAACGGA TATATAAATG CAAAAACTGC ATAACCACTT TAACTAATAC TTTCAACATT
     4321 TTCGGTTTGT ATTACTTCTT ATTCAAATGT AATAAAAGTA TCAACAAAAA ATTGTTAATA
     4381 TACCTCTATA CTTTAACGTC AAGGAGAAAA AACCCCGGAT TCTAGAACTA GTGGATCCCC
     4441 CGGGaaaaaa ATGGACAAGA AGTACTCCAT TGGGCTCGAT ATCGGCACAA ACAGCGTCGG
     4501 tTGGGCCGTC ATTACGGACG AGTACAAGGT GCCGAGCAAA AAATTCAAAG TTCTGGGCAA
     4561 TACCGATCGC CACAGCATAA AGAAGAACCT CATTGGCGCC CTCCTGTTCG ACTCCGGGGA
     4621 GACGGCCGAA GCCACGCGGC TCAAAAGAAC AGCACGGCGC AGATATACCC GCAGAAAGAA
     4681 TCGGATCTGC TACCTGCAGG AGATCTTTAG TAATGAGATG GCTAAGGTGG ATGACTCTTT
     4741 CTTCCATAGG CTGGAGGAGT CCTTTTTGGT GGAGGAGGAT AAAAAGCACG AGCGCCACCC
     4801 AATCTTTGGC AATATCGTGG ACGAGGTGGC GTACCATGAA AAGTACCCAA CCATATATCA
     4861 TCTGAGGAAG AAGCTTGTAG ACAGTACTGA TAAGGCTGAC TTGCGGTTGA TCTATCTCGC
     4921 GCTGGCGCAT ATGATCAAAT TTCGGGGACA CTTCCTCATC GAGGGGGACC TGAACCCAGA
     4981 CAACAGCGAT GTCGACAAAC TCTTTATCCA ACTGGTTCAG ACTTACAATC AGCTTTTCGA
     5041 AGAGAACCCG ATCAACGCAT CCGGAGTTGA CGCCAAAGCA ATCCTGAGCG CTAGGCTGTC
     5101 CAAATCCCGG CGGCTCGAAA ACCTCATCGC ACAGCTCCCT GGGGAGAAGA AGAACGGCCT
     5161 GTTTGGTAAT CTTATCGCCC TGTCACTCGG GCTGACCCCC AACTTTAAAT CTAACTTCGA
     5221 CCTGGCCGAA GATGCCAAGC TTCAACTGAG CAAAGACACC TACGATGATG ATCTCGACAA
     5281 TCTGCTGGCC CAGATCGGCG ACCAGTACGC AGACCTTTTT TTGGCGGCAA AGAACCTGTC
     5341 AGACGCCATT CTGCTGAGTG ATATTCTGCG AGTGAACACG GAGATCACCA AAGCTCCGCT
     5401 GAGCGCTAGT ATGATCAAGC GCTATGATGA GCACCACCAA GACTTGACTT TGCTGAAGGC
     5461 CCTTGTCAGA CAGCAACTGC CTGAGAAGTA CAAGGAAATT TTCTTCGATC AGTCTAAAAA
     5521 TGGCTACGCC GGATACATTG ACGGCGGAGC AAGCCAGGAG GAATTTTACA AATTTATTAA
     5581 GCCCATCTTG GAAAAAATGG ACGGCACCGA GGAGCTGCTG GTAAAGCTTA ACAGAGAAGA
     5641 TCTGTTGCGC AAACAGCGCA CTTTCGACAA TGGAAGCATC CCCCACCAGA TTCACCTGGG
     5701 CGAACTGCAC GCTATCCTCA GGCGGCAAGA GGATTTCTAC CCCTTTTTGA AAGATAACAG
     5761 GGAAAAGATT GAGAAAATCC TCACATTTCG GATACCCTAC TATGTAGGCC CCCTCGCCCG
     5821 GGGAAATTCC AGATTCGCGT GGATGACTCG CAAATCAGAA GAGACCATCA CTCCCTGGAA
     5881 CTTCGAGGAA GTCGTGGATA AGGGGGCCTC TGCCCAGTCC TTCATCGAAA GGATGACTAA
     5941 CTTTGATAAA AATCTGCCTA ACGAAAAGGT GCTTCCTAAA CACTCTCTGC TGTACGAGTA
     6001 CTTCACAGTT TATAACGAGC TCACCAAGGT CAAATACGTC ACAGAAGGGA TGAGAAAGCC
     6061 AGCATTCCTG TCTGGAGAGC AGAAGAAAGC TATCGTGGAC CTCCTCTTCA AGACGAACCG
     6121 GAAAGTTACC GTGAAACAGC TCAAAGAAGA CTATTTCAAA AAGATTGAAT GTTTCGACTC
     6181 TGTTGAAATC AGCGGAGTGG AGGATCGCTT CAACGCATCC CTGGGAACGT ATCACGATCT
     6241 CCTGAAAATC ATTAAAGACA AGGACTTCCT GGACAATGAG GAGAACGAGG ACATTCTTGA
     6301 GGACATTGTC CTCACCCTTA CGTTGTTTGA AGATAGGGAG ATGATTGAAG AACGCTTGAA
     6361 AACTTACGCT CATCTCTTCG ACGACAAAGT CATGAAACAG CTCAAGAGGC GCCGATATAC
     6421 AGGATGGGGG CGGCTGTCAA GAAAACTGAT CAATGGGATC CGAGACAAGC AGAGTGGAAA
     6481 GACAATCCTG GATTTTCTTA AGTCCGATGG ATTTGCCAAC CGGAACTTCA TGCAGTTGAT
     6541 CCATGATGAC TCTCTCACCT TTAAGGAGGA CATCCAGAAA GCACAAGTTT CTGGCCAGGG
     6601 GGACAGTCTT CACGAGCACA TCGCTAATCT TGCAGGTAGC CCAGCTATCA AAAAGGGAAT
     6661 ACTGCAGACC GTTAAGGTCG TGGATGAACT CGTCAAAGTA ATGGGAAGGC ATAAGCCCGA
     6721 GAATATCGTT ATCGAGATGG CCCGAGAGAA CCAAACTACC CAGAAGGGAC AGAAGAACAG
     6781 TAGGGAAAGG ATGAAGAGGA TTGAAGAGGG TATAAAAGAA CTGGGGTCCC AAATCCTTAA
     6841 GGAACACCCA GTTGAAAACA CCCAGCTTCA GAATGAGAAG CTCTACCTGT ACTACCTGCA
     6901 GAACGGCAGG GACATGTACG TGGATCAGGA ACTGGACATC AATCGGCTCT CCGACTACGA
     6961 CGTGGATCAT ATCGTGCCCC AGTCTTTTCT CAAAGATGAT TCTATTGATA ATAAAGTGTT
     7021 GACAAGATCC GATAAAAATA GAGGGAAGAG TGATAACGTC CCCTCAGAAG AAGTTGTCAA
     7081 GAAAATGAAA AATTATTGGC GGCAGCTGCT GAACGCCAAA CTGATCACAC AACGGAAGTT
     7141 CGATAATCTG ACTAAGGCTG AACGAGGTGG CCTGTCTGAG TTGGATAAAG CCGGCTTCAT
     7201 CAAAAGGCAG CTTGTTGAGA CACGCCAGAT CACCAAGCAC GTGGCCCAAA TTCTCGATTC
     7261 ACGCATGAAC ACCAAGTACG ATGAAAATGA CAAACTGATT CGAGAGGTGA AAGTTATTAC
     7321 TCTGAAGTCT AAGCTGGTCT CAGATTTCAG AAAGGACTTT CAGTTTTATA AGGTGAGAGA
     7381 GATCAACAAT TACCACCATG CGCATGATGC CTACCTGAAT GCAGTGGTAG GCACTGCACT
     7441 TATCAAAAAA TATCCCAAGC TTGAATCTGA ATTTGTTTAC GGAGACTATA AAGTGTACGA
     7501 TGTTAGGAAA ATGATCGCAA AGTCTGAGCA GGAAATAGGC AAGGCCACCG CTAAGTACTT
     7561 CTTTTACAGC AATATTATGA ATTTTTTCAA GACCGAGATT ACACTGGCCA ATGGAGAGAT
     7621 TCGGAAGCGA CCACTTATCG AAACAAACGG AGAAACAGGA GAAATCGTGT GGGACAAGGG
     7681 TAGGGATTTC GCGACAGTCC GGAAGGTCCT GTCCATGCCG CAGGTGAACA TCGTTAAAAA
     7741 GACCGAAGTA CAGACCGGAG GCTTCTCCAA GGAAAGTATC CTCCCGAAAA GGAACAGCGA
     7801 CAAGCTGATC GCACGCAAAA AAGATTGGGA CCCCAAGAAA TACGGCGGAT TCGATTCTCC
     7861 TACAGTCGCT TACAGTGTAC TGGTTGTGGC CAAAGTGGAG AAAGGGAAGT CTAAAAAACT
     7921 CAAAAGCGTC AAGGAACTGC TGGGCATCAC AATCATGGAG CGATCAAGCT TCGAAAAAAA
     7981 CCCCATCGAC TTTCTCGAGG CGAAAGGATA TAAAGAGGTC AAAAAAGACC TCATCATTAA
     8041 GCTTCCCAAG TACTCTCTCT TTGAGCTTGA AAACGGCCGG AAACGAATGC TCGCTAGTGC
     8101 GGGCGAGCTG CAGAAAGGTA ACGAGCTGGC ACTGCCCTCT AAATACGTTA ATTTCTTGTA
     8161 TCTGGCCAGC CACTATGAAA AGCTCAAAGG GTCTCCCGAA GATAATGAGC AGAAGCAGCT
     8221 GTTCGTGGAA CAACACAAAC ACTACCTTGA TGAGATCATC GAGCAAATAA GCGAATTCTC
     8281 CAAAAGAGTG ATCCTCGCCG ACGCTAACCT CGATAAGGTG CTTTCTGCTT ACAATAAGCA
     8341 CAGGGATAAG CCCATCAGGG AGCAGGCAGA AAACATTATC CACTTGTTTA CTCTGACCAA
     8401 CTTGGGCGCG CCTGCAGCCT TCAAGTACTT CGACACCACC ATAGACAGAA AGCGGTACAC
     8461 CTCTACAAAG GAGGTCCTGG ACGCCACACT GATTCATCAG TCAATTACGG GGCTCTATGA
     8521 AACAAGAATC GACCTCTCTC AGCTCGGTGG AGACAGCAGG GCTGACCCCA AGAAGAAGAG
     8581 GAAGGTGTGA TCTCTTCTCG AGTCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT
     8641 CCCCCCACAT CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT
     8701 ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT
     8761 TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG
     8821 GTTTTGGGAC GCTCGAAGGC TTTAATTTGC GGCCGgtacc caattcgccc tatagtgagt
     8881 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8941 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9001 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg
     9061 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9121 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9181 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9241 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9301 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9361 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9421 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9481 cgaattttaa caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca
     9541 tctgtgcggt atttcacacc gcatatcgac ggtcgaggag aacttctagt atatccacat
     9601 acctaatatt attgccttat taaaaatgga atcccaacaa ttacatcaaa atccacattc
     9661 tcttcaaaat caattgtcct gtacttcctt gttcatgtgt gttcaaaaac gttatattta
     9721 taggataatt atactctatt tctcaacaag taattggttg tttggccgag cggtctaagg
     9781 cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag
     9841 atgcaagagt tcgaatctct tagcaaccat tatttttttc ctcaacataa cgagaacaca
     9901 caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag
     9961 gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag
    10021 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
    10081 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
    10141 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatttc
    10201 aaggatatac cattctaatg tctgccccta tgtctgcccc taagaagatc gtcgttttgc
    10261 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
    10321 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
    10381 tcgatgctac aggtgtccca cttccagatg aggcgctgga agcctccaag aaggttgatg
    10441 ccgttttgtt aggtgctgtg ggtggtccta aatggggtac cggtagtgtt agacctgaac
    10501 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
    10561 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
    10621 acttcgttgt tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg
    10681 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
    10741 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
    10801 ataaagctaa tgttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
    10861 agaacgaatt ccctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc
    10921 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
    10981 tcatctccga tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct
    11041 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg
    11101 ctccagattt gccaaagaat aaggtCAacc ctatcgccac tatcttgtct gctgcaatga
    11161 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa
    11221 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
    11281 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt
    11341 tatgatattt gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac
    11401 aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat acatttatca
    11461 agaaggagaa aaaggaggat gtaaaggaat acaggtaagc aaattgatac taatggctca
    11521 acgtgataag gaaaaagaat tgcactttaa cattaatatt gacaaggagg agggcaccac
    11581 acaaaaagtt aggtgtaaca gaaaatcatg aaactaTgat tcctaatttA Tatattggag
    11641 gattttctct aaaaaaaaaa aaatacaaca aataaaaaac actcaatgac ctgaccattt
    11701 gatggagttt aagtcaatac cttcttgaac catttcccat aatggtgaaa gttccctcaa
    11761 gaattttact ctgtcagaaa cggccttacg acgtagtcga tatggtgcac tctcagtaca
    11821 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
    11881 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    11941 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga 
//
