LOCUS       CSWAT_SMASH             8115 bp    DNA        circular     27-JAN-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(5339..5358)
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3586..3615)
                     /locus_tag="I-Sce1 18 n Restriction site"
                     /label="I-Sce1 18 n Restriction site"
                     /ApEinfo_label="I-Sce1 18 n Restriction site"
                     /ApEinfo_fwdcolor="#eeff73"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3616..3705
                     /locus_tag="N-Terminus of Sir4"
                     /label="N-Terminus of Sir4"
                     /ApEinfo_label="N-Terminus of Sir4"
                     /ApEinfo_fwdcolor="#d84bfe"
                     /ApEinfo_revcolor="#d84bfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2478..2495
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3567..3585
                     /locus_tag="S2-Site"
                     /label="S2-Site"
                     /ApEinfo_label="S2-Site"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5376..5396)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2614..2631
                     /locus_tag="S1/S3-Site"
                     /label="S1/S3-Site"
                     /ApEinfo_label="S1/S3-Site"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2504..2524
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3586..3615)
                     /locus_tag="I-SceI site"
                     /label="I-SceI site"
                     /ApEinfo_label="I-SceI site"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5764..6446)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2592..2613)
                     /locus_tag="I-SceI site(1)"
                     /label="I-SceI site(1)"
                     /ApEinfo_label="I-SceI site"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2011..2317)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2614..2658
                     /locus_tag="L3 Linker Sequence"
                     /label="L3 Linker Sequence"
                     /ApEinfo_label="L3 Linker Sequence"
                     /ApEinfo_fwdcolor="#fb244b"
                     /ApEinfo_revcolor="#fb244b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1879..2334)
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3541..3585
                     /locus_tag="L4 Linker Sequence"
                     /label="L4 Linker Sequence"
                     /ApEinfo_label="L4 Linker Sequence"
                     /ApEinfo_fwdcolor="#67ff4c"
                     /ApEinfo_revcolor="#67ff4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2339..2407)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(5402..5424)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6544..7203)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4123..4932
                     /locus_tag="Kan Resistance Gene"
                     /label="Kan Resistance Gene"
                     /ApEinfo_label="Kan Resistance Gene"
                     /ApEinfo_fwdcolor="#7174ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5186..5232
                     /locus_tag="End of SIR4 ORF"
                     /label="End of SIR4 ORF"
                     /ApEinfo_label="End of SIR4 ORF"
                     /ApEinfo_fwdcolor="#fb3350"
                     /ApEinfo_revcolor="#fb3350"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7533..8043
                     /locus_tag="CEN-ARS pRS"
                     /label="CEN-ARS pRS"
                     /ApEinfo_label="CEN-ARS pRS"
                     /ApEinfo_fwdcolor="#ff6fcf"
                     /ApEinfo_revcolor="#ff6fcf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5048..5065
                     /locus_tag="Reverse Primer"
                     /label="Reverse Primer"
                     /ApEinfo_label="Reverse Primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(440..1774)
                     /locus_tag="Met15 ORF"
                     /label="Met15 ORF"
                     /ApEinfo_label="Met15 ORF"
                     /ApEinfo_fwdcolor="#6851ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5166..5185
                     /locus_tag="R1"
                     /label="R1"
                     /ApEinfo_label="R1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(157..227)
                     /locus_tag="Gap Repair Primer"
                     /label="Gap Repair Primer"
                     /ApEinfo_label="Gap Repair Primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3706..3725
                     /locus_tag="F1"
                     /label="F1"
                     /ApEinfo_label="F1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1808..1878)
                     /locus_tag="Other Gap Repair Primer"
                     /label="Other Gap Repair Primer"
                     /ApEinfo_label="Other Gap Repair Primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3826..3844
                     /locus_tag="KAN R Primer"
                     /label="KAN R Primer"
                     /ApEinfo_label="KAN R Primer"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5276..5305)
                     /locus_tag="I-SceI site(2)"
                     /label="I-SceI site(2)"
                     /ApEinfo_label="I-SceI site"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5276..5305)
                     /locus_tag="I-Sce1 18 n Restriction site(1)"
                     /label="I-Sce1 18 n Restriction site(1)"
                     /ApEinfo_label="I-Sce1 18 n Restriction site"
                     /ApEinfo_fwdcolor="#eeff73"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5233..5275
                     /locus_tag="SIR4 3' Homology Flank"
                     /label="SIR4 3' Homology Flank"
                     /ApEinfo_label="SIR4 3' Homology Flank"
                     /ApEinfo_fwdcolor="#7b9efe"
                     /ApEinfo_revcolor="#7b9efe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4802..4823
                     /locus_tag="KAN_SEQ_F"
                     /label="KAN_SEQ_F"
                     /ApEinfo_label="KAN_SEQ_F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2391..2411
                     /locus_tag="Sir4_tag_SEQ_F"
                     /label="Sir4_tag_SEQ_F"
                     /ApEinfo_label="Sir4_tag_SEQ_F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2659..3540
                     /locus_tag="SMASH TAG"
                     /label="SMASH TAG"
                     /ApEinfo_label="SMASH TAG"
                     /ApEinfo_fwdcolor="#ff4ff1"
                     /ApEinfo_revcolor="#ff4ff1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2546..2572
                     /locus_tag="CSWAT_Plasmid_F 964"
                     /label="CSWAT_Plasmid_F 964"
                     /ApEinfo_label="CSWAT_Plasmid_F 964"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2586..2612
                     /locus_tag="IAA17_F 959"
                     /label="IAA17_F 959"
                     /ApEinfo_label="IAA17_F 959"
                     /ApEinfo_fwdcolor="#ffbf61"
                     /ApEinfo_revcolor="#ffbf61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCACGCTTT TCAATTCAAT TCATCACCAT CCTCATGAAA ACTGTGTAAC ATAATAACCG
      241 AAGTGTCGAA AAGGTGGCAC CTTGTCCAAT TGAACACGCT CGATGAAAAA AATAAGATAT
      301 ATATAAGGTT AAGTAAAGCG TCTGTTAGAA AGGAAGTTTT TCCTTTTTCT TGCTCTCTTG
      361 TCTTTTCATC TACTATTTCC TTCGTGTAAT ACAGGGTCGT CAGATACATA GATACAATTC
      421 TATTACCCCC ATCCATACAA TGCCATCTCA TTTCGATACT GTTCAACTAC ACGCCGGCCA
      481 AGAGAACCCT GGTGACAATG CTCACAGATC CAGAGCTGTA CCAATTTACG CCACCACTTC
      541 TTATGTTTTC GAAAACTCTA AGCATGGTTC GCAATTGTTT GGTCTAGAAG TTCCAGGTTA
      601 CGTCTATTCC CGTTTCCAAA ACCCAACCAG TAATGTTTTG GAAGAAAGAA TTGCTGCTTT
      661 AGAAGGTGGT GCTGCTGCTT TGGCTGTTTC CTCCGGTCAA GCCGCTCAAA CCCTTGCCAT
      721 CCAAGGTTTG GCACACACTG GTGACAACAT CGTTTCCACT TCTTACTTAT ACGGTGGTAC
      781 TTATAACCAG TTCAAAATCT CGTTCAAAAG ATTTGGTATC GAGGCTAGAT TTGTTGAAGG
      841 TGACAATCCA GAAGAATTCG AAAAGGTCTT TGATGAAAGA ACCAAGGCTG TTTATTTGGA
      901 AACCATTGGT AATCCAAAGT ACAATGTTCC GGATTTTGAA AAAATTGTTG CAATTGCTCA
      961 CAAACACGGT ATTCCAGTTG TCGTTGACAA CACATTTGGT GCCGGTGGTT ACTTCTGTCA
     1021 GCCAATTAAA TACGGTGCTG ATATTGTAAC ACATTCTGCT ACCAAATGGA TTGGTGGTCA
     1081 TGGTACTACT ATCGGTGGTA TTATTGTTGA CTCTGGTAAG TTCCCATGGA AGGACTACCC
     1141 AGAAAAGTTC CCTCAATTCT CTCAACCTGC CGAAGGATAT CACGGTACTA TCTACAATGA
     1201 AGCCTACGGT AACTTGGCAT ACATCGTTCA TGTTAGAACT GAACTATTAA GAGATTTGGG
     1261 TCCATTGATG AACCCATTTG CCTCTTTCTT GCTACTACAA GGTGTTGAAA CATTATCTTT
     1321 GAGAGCTGAA AGACACGGTG AAAATGCATT GAAGTTAGCC AAATGGTTAG AACAATCCCC
     1381 ATACGTATCT TGGGTTTCAT ACCCTGGTTT AGCATCTCAT TCTCATCATG AAAATGCTAA
     1441 GAAGTATCTA TCTAACGGTT TCGGTGGTGT CTTATCTTTC GGTGTAAAAG ACTTACCAAA
     1501 TGCCGACAAG GAAACTGACC CATTCAAACT TTCTGGTGCT CAAGTTGTTG ACAATTTAAA
     1561 GCTTGCCTCT AACTTGGCCA ATGTTGGTGA TGCCAAGACC TTAGTCATTG CTCCATACTT
     1621 CACTACCCAC AAACAATTAA ATGACAAAGA AAAGTTGGCA TCTGGTGTTA CCAAGGACTT
     1681 AATTCGTGTC TCTGTTGGTA TCGAATTTAT TGATGACATT ATTGCAGACT TCCAGCAATC
     1741 TTTTGAAACT GTTTTCGCTG GCCAAAAACC ATGAGTGTGC GTAATGAGTT GTAAAATTAT
     1801 GTATAAACCT ACTTTCTCTC ACAAGTACTG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
     1861 AGAAAATACC GCATCAGGaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc
     2341 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2401 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2461 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     2521 agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
     2581 aattcctgca gCTATATTAC CCTGTTATCC CTACGTACGC TGCAGGTCGA Cggtggcggt
     2641 tctggcggtg gcggatccga cgaaatggaa gaatgtagta agcacttacc tggtgcaggc
     2701 tcatctggcg atatcatgga ctacaaggac gacgatgata agggttcttc tggaacaggt
     2761 tcaggttcag gtacaagtgc acccattact gcttatgctc agcaaacaag aggcctattg
     2821 ggttgtatta ttactagttt gacgggcaga gacaaaaacc aggtagaagg tgaagtgcag
     2881 atcgtatcca cggctactca gacattcttg gctacttgta ttaatggtgt gtgttgggca
     2941 gtttaccacg gtgcgggaac tagaactatt gcctctccta agggtccagt gatccaaatg
     3001 tatactaacg ttgatcaaga tttggtcggc tggccagctc cacaagggtc aagaagccta
     3061 actccttgca cctgcggttc ttcagatctg tacttagtga cacgtcatgc agatgttata
     3121 ccagttagac gtcgtgggga cagtagaggt tcactgctgt caccacgtcc aattagttac
     3181 cttaaaggta gttctggcgg tcccctgttg tgtcctgcag gtcatgcagt aggattgttt
     3241 agagccgccg tctgcaccag aggggttgca aaagctgtgg attttattcc tgttgaaaat
     3301 cttgaaacga ctatgagatc acctgtcttt accgataata gttccccacc agctgtgact
     3361 cttacacatc cgatcaccaa aatcgatact aaatacatta tgacctgtat gtctgccgat
     3421 ttagaagtag taacatctac atgggtttta gtcggtgggg ttttagctgc cctagccgca
     3481 tattgcttgt ccacaggttg cgttgttata gttggcagga tcgttttgag cggaaaataa
     3541 agttcttctt tgagatatcg attgaacgag ctcgaattcA TCGATCTATA TTACCCTGTT
     3601 ATCCCTAGCG TAACTATGCC AAATGACAAT AAGACACCCA ATAGGTCCAG CACTCCCAAG
     3661 TTTACTAAAA AACCAGTAAC CCCGAATGAT AAAATTCCTG AAAGACGGAT CCCCGGGTTA
     3721 ATTAAGGCGC GCCAGATCTG TTTAGCTTGC CTCGTCCCCG CCGGGTCACC CGGCCAGCGA
     3781 CATGGAGGCC CAGAATACCC TCCTTGACAG TCTTGACGTG CGCAGCTCAG GGGCATGATG
     3841 TGACTGTCGC CCGTACATTT AGCCCATACA TCCCCATGTA TAATCATTTG CATCCATACA
     3901 TTTTGATGGC CGCACGGCGC GAAGCAAAAA TTACGGCTCC TCGCTGCAGA CCTGCGAGCA
     3961 GGGAAACGCT CCCCTCACAG ACGCGTTGAA TTGTCCCCAC GCCGCGCCCC TGTAGAGAAA
     4021 TATAAAAGGT TAGGATTTGC CACTGAGGTT CTTCTTTCAT ATACTTCCTT TTAAAATCTT
     4081 GCTAGGATAC AGTTCTCACA TCACATCCGA ACATAAACAA CCATGGGTAA GGAAAAGACT
     4141 CACGTTTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG
     4201 GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT
     4261 GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG
     4321 ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC
     4381 CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGCAAAAC AGCATTCCAG
     4441 GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG
     4501 CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT
     4561 CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC
     4621 GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC
     4681 TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG
     4741 GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT
     4801 CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT
     4861 CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT
     4921 GAGTTTTTCT AATCAGTACT GACAATAAAA AGATTCTTGT TTTCAAGAAC TTGTCATTTG
     4981 TATAGTTTTT TTATATTGTA GTTGTTCTAT TTTAATCAAA TGTTAGCGTG ATTTATATTT
     5041 TTTTTCGCCT CGACATCATC TGCCCAGATG CGAAGTTAAG TGCGCAGAAA GTAATATCAT
     5101 GCGTCAATCG TATGTGAATG CTGGTCGCTA TACTGCTGTC GATTCGATAC TAACGCCGCC
     5161 ATCCAGTTTA AACGAGCTCG AATTCTGATG GAAAAAGATT TTCAAGTGAA TAAGGAGATA
     5221 AAACCGTATT GACTTTTTAT CATTCTACAA AAGACCAGTA ACGAAGTGTA CCCTGCTATA
     5281 TTACCCTGTT ATCCCTAGCG TAACTcggcc gccaccgcgg tggagctcca gcttttgttc
     5341 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5401 aaattgttat ccgctcacaa ttccacacaa cataggagcc ggaagcataa agtgtaaagc
     5461 ctggggtgcc taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt
     5521 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5581 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5641 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5701 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5761 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6181 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7501 tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg ctataaaaat
     7561 aattataatt taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga
     7621 aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca
     7681 acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca
     7741 tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt
     7801 aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac
     7861 gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt
     7921 ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag
     7981 agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa
     8041 gcgatccgtc ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8101 cgaggccctt tcgtc    
//
