LOCUS       Exported                9162 bp DNA     circular SYN 05-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 7, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..9162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    853..1566
                     /locus_tag="YPET"
                     /label=YPET
     misc_feature    1573..1995
                     /locus_tag="FHA1 DOMAIN"
                     /label=FHA1 DOMAIN
     misc_feature    2002..2349
                     /locus_tag="EV linker"
                     /label=EV linker
     misc_feature    2368..2370
                     /locus_tag="Mutated from ACG to GCC (T->A)"
                     /label=Mutated from ACG to GCC (T->A)
     misc_feature    2389..3111
                     /locus_tag="eCFP"
                     /label=eCFP
     misc_feature    3112..3150
                     /locus_tag="NES"
                     /label=NES
     misc_feature    5417..6216
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    6648..7508
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    8591..8620
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cacggagctc
      841 actgaattcg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      901 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      961 gatgccacct acggcaagct gaccctgaag cttctatgca ccaccggcaa gctgcccgtg
     1021 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     1081 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1141 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1201 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1261 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1321 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1441 cccgacaacc actacctgag ctaccagtcc gccctgttca aagaccccaa cgagaagcgc
     1501 gatcacatgg tcctgctgga gttcctgacc gccgccggga tcactgaggg catgaacgag
     1561 ctgtacctcg agaagttttc tcaagaacag atcggcgaaa acattgtgtg cagggtcatt
     1621 tgtaccacgg gtcaaattcc catccgagat ttgtcagctg atatttcaca agtgcttaag
     1681 gaaaaacgat ccataaagaa agtttggaca tttggtagaa acccagcctg tgactatcat
     1741 ttaggaaaca tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt
     1801 aaccttttat tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag
     1861 aagaacagca atcagttact gtcccaaggt gatgaaataa ccgttggtgt aggcgtggaa
     1921 tcagatattt tatctctggt cattttcata aacgacaaat ttaagcagtg cctggagcag
     1981 aacaaagttg atcgcggtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     2041 gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt
     2101 gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc
     2161 ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt
     2221 gctggtggta gtgctggtgg tagtgctggt ggttccggca gtgctggtgg tagtgctggt
     2281 ggtagtacca gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt
     2341 gctggtggtt ccggattgag gcgcgcggcc ctggttgacg gcggccgcat ggtgagcaag
     2401 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2461 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2521 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2581 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2641 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2701 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2761 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2821 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     2881 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2941 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cttgagcacc
     3001 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3061 gtgaccgccg ccgggatcac tctcggcatg gacgagctgg gccgctctag actgcagctg
     3121 cctcctctgg aacgcctgac tctggattaa agtgtcgacc gtggatccag ctttggactt
     3181 cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga
     3241 aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa
     3301 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     3361 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga naattcttat tcttgagtaa
     3421 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     3481 cacctctacc ggcatgccaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3541 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3601 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3661 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataatcgga
     3721 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     3781 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     3841 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     3901 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     3961 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     4021 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     4081 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     4141 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     4201 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     4261 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     4321 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     4381 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     4441 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     4501 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     4561 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     4621 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     4681 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     4741 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     4801 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     4861 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     4921 agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     4981 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     5041 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     5101 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     5161 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     5221 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     5281 tcatttcctt tgatattgga tcgatccgat gataagctgt caaacatgag aattgggtaa
     5341 taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta
     5401 taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt
     5461 tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac
     5521 aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa
     5581 tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc
     5641 atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt
     5701 cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa
     5761 ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa
     5821 accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc
     5881 tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt
     5941 tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt
     6001 gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg
     6061 acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca
     6121 caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg
     6181 agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcatgt
     6241 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     6301 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     6361 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     6421 tgatgaattg aaaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     6481 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     6541 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6601 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6661 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6721 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6781 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6841 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6901 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6961 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7021 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7081 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7141 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7201 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7261 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7321 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7381 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7441 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7501 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7561 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7621 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7681 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7741 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7801 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7861 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7921 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7981 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8041 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8101 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8161 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8221 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8341 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8401 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8461 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8521 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8581 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8641 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttacc gcatcaggaa
     8701 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8761 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8821 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8881 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8941 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     9001 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     9061 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     9121 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gc
//
