LOCUS       PX552-mScn2a-Hal        6209 bp    DNA     circular     16-SEP-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-12994161|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    165..397
                     /vntifkey="21"
                     /label=U6p
     misc_feature    2954..3432
                     /vntifkey="21"
                     /label=hGH\PA\terminator
     misc_feature    4529..5389
                     /vntifkey="21"
                     /label=Amp
     misc_feature    2196..2903
                     /vntifkey="21"
                     /label=sfGFP
     enhancer        1565..2180
                     /vntifkey="9"
                     /label=EF1
     misc_feature    405..424
                     /vntifkey="21"
                     /label=mScn2a\gRNA
     misc_feature    519..541
                     /vntifkey="21"
                     /label=mScn2a\target\gRNA1
     misc_feature    1536..1558
                     /vntifkey="21"
                     /label=mScn2a\target\gRNA2
     misc_feature    557..1477
                     /vntifkey="21"
                     /label=HaloTag
     V_region        1478..1519
                     /vntifkey="45"
                     /label=V5
BASE COUNT     1427 a      1638 c      1589 g      1555 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt 
      181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa 
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 
      361 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggacaa ggggaaagat 
      421 atcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa 
      481 aagtggcacc gagtcggtgc tttttttcta gtaccggtcc ctgatatctt tccccttgtc 
      541 cggttctgga tcgggtgagg atctgtactt tcagagcgat aacgatggat ccgaaatcgg 
      601 tactggcttt ccattcgacc cccattatgt ggaagtcctg ggcgagcgca tgcactacgt 
      661 cgatgttggt ccgcgcgatg gcacccctgt gctgttcctg cacggtaacc cgacctcctc 
      721 ctacgtgtgg cgcaacatca tcccgcatgt tgcaccgacc catcgctgca ttgctccaga 
      781 cctgatcggt atgggcaaat ccgacaaacc agacctgggt tatttcttcg acgaccacgt 
      841 ccgcttcatg gatgccttca tcgaagccct gggtctggaa gaggtcgtcc tggtcattca 
      901 cgactggggc tccgctctgg gtttccactg ggccaagcgc aatccagagc gcgtcaaagg 
      961 tattgcattt atggagttca tccgccctat cccgacctgg gacgaatggc cagaatttgc 
     1021 ccgcgagacc ttccaggcct tccgcaccac cgacgtcggc cgcaagctga tcatcgatca 
     1081 gaacgttttt atcgagggta cgctgccgat gggtgtcgtc cgcccgctga ctgaagtcga 
     1141 gatggaccat taccgcgagc cgttcctgaa tcctgttgac cgcgagccac tgtggcgctt 
     1201 cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc gtcgcgctgg tcgaagaata 
     1261 catggactgg ctgcaccagt cccctgtccc gaagctgctg ttctggggca ccccaggcgt 
     1321 tctgatccca ccggccgaag ccgctcgcct ggccaaaagc ctgcctaact gcaaggctgt 
     1381 ggacatcggc ccgggtctga atctgctgca agaagacaac ccggacctga tcggcagcga 
     1441 gatcgcgcgc tggctgtcta ctctggagat ttccggtggg aagcctatac ccaatcctct 
     1501 cctggggttg gacagcaccg gctcaggtag cggctccctg atatctttcc ccttgtcctc 
     1561 tagaccaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 
     1621 agtccccgag aagttggggg gaggggtcgg caattcgctc cggtgcccgt cagtgggcag 
     1681 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg 
     1741 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 
     1801 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 
     1861 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac 
     1921 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc 
     1981 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga 
     2041 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc 
     2101 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt 
     2161 acagatccaa gctgtgaccg ggtaccgcca ccatgaaagg agaagaactt ttcactggag 
     2221 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtccgtg 
     2281 gagagggtga aggtgatgct acaaacggaa aactcaccct taaatttatt tgcactactg 
     2341 gaaaactacc tgttccgtgg ccaacacttg tcactactct gacctatggt gttcaatgct 
     2401 tttcccgtta tccggatcac atgaaacggc atgacttttt caagagtgcc atgcccgaag 
     2461 gttatgtaca ggaacgcact atatctttca aagatgacgg gacctacaag acgcgtgctg 
     2521 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaagggt attgatttta 
     2581 aagaagatgg aaacattctt ggacacaaac tcgagtacaa ctttaactca cacaatgtat 
     2641 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaacg 
     2701 ttgaagatgg ttccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 
     2761 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgtcctt tcgaaagatc 
     2821 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac 
     2881 atggcatgga tgagctctac aaataagaat tcgatatcaa gcttatcgat accgagcgct 
     2941 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg 
     3001 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg 
     3061 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg 
     3121 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca 
     3181 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct 
     3241 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt 
     3301 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag 
     3361 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc 
     3421 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc 
     3481 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 
     3541 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 
     3601 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 
     3661 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 
     3721 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     3781 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     3841 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     3901 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     3961 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     4021 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     4081 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 
     4141 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 
     4201 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 
     4261 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 
     4321 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 
     4381 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     4441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     4501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
     4561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
     4621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
     4681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
     4741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
     4801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
     4861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
     4921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
     4981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
     5041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
     5101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
     5161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
     5221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
     5281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
     5341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     5401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     5461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     5521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     5581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     5641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     5701 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     5761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     5821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     5881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     5941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     6001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     6061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     6121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     6181 ggccttttgc tggccttttg ctcacatgt 
//
