LOCUS       PX552-mScn8a-Hal        6206 bp    DNA     circular     31-JAN-2022
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14235237|
COMMENT     VNTDBDATE|-14243849|
COMMENT     LSOWNER|
COMMENT     VNTNAME|PX552-mScn8a-HaloTag-V5-EF1-sfGFP|
COMMENT     VNTAUTHORNAME|Hui Liu|
COMMENT     VNTOAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    165..397
                     /vntifkey="21"
                     /label=U6p
     misc_feature    2951..3429
                     /vntifkey="21"
                     /label=hGH\PA\terminator
     misc_feature    4526..5386
                     /vntifkey="21"
                     /label=Amp
     enhancer        1562..2177
                     /vntifkey="9"
                     /label=EF1
     misc_feature    405..424
                     /vntifkey="21"
                     /label=mScn8a\gRNA
     misc_feature    519..541
                     /vntifkey="21"
                     /label=mScn8a\gRNA\target\site1
     misc_feature    1533..1555
                     /vntifkey="21"
                     /label=mScn8a\gRNA\target\site2
     misc_feature    555..1517
                     /vntifkey="21"
                     /label=HaloTag-V5
     misc_feature    2193..2900
                     /vntifkey="21"
                     /label=sfGFP
BASE COUNT     1422 a      1639 c      1595 g      1550 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt 
      181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa 
      241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 
      301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 
      361 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccccgaca aggagaagca 
      421 gcaggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa 
      481 aagtggcacc gagtcggtgc tttttttcta gtaccggtcc gctgctgctt ctccttgtcg 
      541 gttctggatc gggtgaggat ctgtactttc agagcgataa cgatggatcc gaaatcggta 
      601 ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg cactacgtcg 
      661 atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg acctcctcct 
      721 acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt gctccagacc 
      781 tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac gaccacgtcc 
      841 gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg 
      901 actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta 
      961 ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca gaatttgccc 
     1021 gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc atcgatcaga 
     1081 acgtttttat cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga 
     1141 tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc 
     1201 caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc gaagaataca 
     1261 tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc ccaggcgttc 
     1321 tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg 
     1381 acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga 
     1441 tcgcgcgctg gctgtctact ctggagattt ccggtgggaa gcctataccc aatcctctcc 
     1501 tggggttgga cagcaccggc tcaggtagcg gcccgctgct gcttctcctt gtcggtctag 
     1561 accaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 
     1621 ccccgagaag ttggggggag gggtcggcaa ttcgctccgg tgcccgtcag tgggcagagc 
     1681 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 
     1741 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 
     1801 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 
     1861 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 
     1921 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 
     1981 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 
     2041 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 
     2101 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 
     2161 gatccaagct gtgaccgggt accgccacca tgaaaggaga agaacttttc actggagttg 
     2221 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag 
     2281 agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa 
     2341 aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt 
     2401 cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt 
     2461 atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag 
     2521 tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag 
     2581 aagatggaaa cattcttgga cacaaactcg agtacaactt taactcacac aatgtataca 
     2641 tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg 
     2701 aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc 
     2761 ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca 
     2821 acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg 
     2881 gcatggatga gctctacaaa taagaattcg atatcaagct tatcgatacc gagcgctgct 
     2941 cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 
     3001 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 
     3061 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 
     3121 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 
     3181 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 
     3241 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 
     3301 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 
     3361 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 
     3421 cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag 
     3481 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa 
     3541 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct 
     3601 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 
     3661 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     3721 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     3781 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     3841 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt 
     3901 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     3961 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     4021 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     4081 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt 
     4141 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc 
     4201 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 
     4261 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 
     4321 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 
     4381 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     4441 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     4501 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 
     4561 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 
     4621 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 
     4681 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 
     4741 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 
     4801 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 
     4861 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 
     4921 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 
     4981 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 
     5041 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 
     5101 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 
     5161 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 
     5221 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 
     5281 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 
     5341 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 
     5401 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     5461 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     5521 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     5581 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     5641 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     5701 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     5761 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     5821 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     5881 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     5941 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     6001 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     6061 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     6121 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     6181 cttttgctgg ccttttgctc acatgt 
//
