LOCUS       Exported                4056 bp DNA     circular SYN 31-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 31, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4056
                     /note="/dnas_title=NB MCS-1"
                     /organism="synthetic DNA construct"
     promoter        27..779
                     /note="HSV-TK"
     misc_feature    821..829
                     /label=Kozak sequence
     misc_feature    830..1039
                     /label=Endofin domain
     misc_feature    1040..1093
                     /label=Linker
     gene            1094..1567
                     /label=LgBiT
                     /note="LgBiT"
     misc_feature    1603..1824
                     /label=SV40 late poly(A) region
                     /note="SV40 late poly(A) region"
     rep_origin      2149
                     /label=ColE1-derived
                     /note="ColE1-derived"
     gene            complement(2938..3798)
                     /gene="ampR"
                     /label=ampR
                     /note="/dnas_title=ampR"
     polyA_signal    3903..4056
                     /note="synthetic; transcriptional pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccagatct gccgccacca tgcagaaaca
      841 gcctacttgg gttcctgatt cagaagctcc aaactgtatg aactgccaag tcaaatttac
      901 ttttaccaaa cggcgacacc attgccgagc atgtgggaaa gtattttgtg gtgtctgttg
      961 taataggaag tgtaaactgc aatatctaga aaaggaagca agagtatgtg tagtctgcta
     1021 tgaaactatt agtaaataag ggaattctgg ctcgagcggt ggtggcggga gcggaggtgg
     1081 agggtcgtca ggtgtcttca cactcgaaga tttcgttggg gactgggaac agacagccgc
     1141 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgctgc agaatctcgc
     1201 cgtgtccgta actccgatcc aaaggattgt ccggagcggt gaaaatgccc tgaagatcga
     1261 catccatgtc atcatcccgt atgaaggtct gagcgccgac caaatggccc agatcgaaga
     1321 ggtgtttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgccctatgg
     1381 cacactggta atcgacgggg ttacgccgaa catgctgaac tatttcggac ggccgtatga
     1441 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     1501 caaaattatc gacgagcgcc tgatcacccc cgacggctcc atgctgttcc gagtaaccat
     1561 caacagctaa tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     1621 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1681 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1741 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1801 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc
     1861 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     1921 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     1981 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2041 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2101 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2161 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2221 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2281 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2341 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2401 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2461 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2521 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2581 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2641 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2701 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2761 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2821 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2881 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     2941 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     3001 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     3061 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     3121 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     3181 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     3241 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     3301 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     3361 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     3421 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     3481 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     3541 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     3601 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     3661 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     3721 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     3781 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     3841 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     3901 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     3961 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     4021 tccccagtgc aagtgcaggt gccagaacat ttctct
//
