LOCUS       Exported               10075 bp DNA     circular SYN 01-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 1, 2022 from SnapGene 5.3.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    19..136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        184..1362
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          415..1353
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             1388..2095
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2102..3415
                     /codon_start=1
                     /label=hTRF1
                     /translation="AEHVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLE
                     CQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSA
                     EAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKE
                     HDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDT
                     FHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGND
                     VEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERR
                     VGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGE
                     GNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED"
     protein_bind    complement(3441..3465)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3555..4016
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4021..4440
                     /codon_start=1
                     /label=BlastR
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     misc_feature    4478..5066
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4949..4960)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5214..5394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        5527..5631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5632..6492
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6663..7251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7662..7683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7698..7728
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7736..7752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7760..7776
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8037..8670
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8717..8842
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9339..9572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      181 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      241 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      301 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      361 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      421 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      481 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      541 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      601 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      661 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      721 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      781 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      841 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      901 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      961 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1021 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1081 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1141 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1201 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1261 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1321 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccg cccccttcac
     1381 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     1441 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     1501 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     1561 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     1621 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     1681 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     1741 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     1801 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     1861 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     1921 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     1981 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     2041 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggatc
     2101 cgcggagcat gtttcctcag cggccccgag cccgcggggc tgtgcggatg gtagggatgc
     2161 cgaccctact gaggagcaga tggcagaaac agagagaaac gacgaggagc agttcgaatg
     2221 ccaggaactg ctcgagtgcc aggtgcaggt gggggccccc gaggaggagg aggaggagga
     2281 ggaggacgcg ggcctggtgg ccgaggccga ggccgtggct gccggctgga tgctcgattt
     2341 cctctgcctc tctctttgcc gagctttccg cgacggccgc tccgaggact tccgcaggac
     2401 ccgcaacagc gcagaggcta ttattcatgg actatccagt ctaacagctt gccagttgag
     2461 aacgatatac atatgtcagt ttttgacaag aattgcagca ggaaaaaccc ttgatgcaca
     2521 gtttgaaaat gatgaacgaa ttacaccctt ggaatcagcc ctgatgattt ggggttcaat
     2581 tgaaaaggaa catgacaaac ttcatgaaga aatacagaat ttaattaaaa ttcaggctat
     2641 agctgtttgt atggaaaatg gcaactttaa agaagcagaa gaagtctttg aaagaatatt
     2701 tggtgatcca aattctcata tgcctttcaa aagcaaattg cttatgataa tctctcagaa
     2761 agatacattt cattcctttt ttcaacactt cagctacaac cacatgatgg agaaaattaa
     2821 gagttatgtg aattatgtgc taagtgaaaa atcatcaacc tttctaatga aggcagcggc
     2881 aaaagtagta gaaagcaaaa ggacaagaac aataacttct caagataaac ctagtggtaa
     2941 tgatgttgaa atggaaactg aagctaattt ggatacaaga aaaagtgtta gtgacaaaca
     3001 gtctgcggta actgaatcct cagagggtac agtatcctta ttgaggtctc acaagaatct
     3061 tttcttatct aagttgcaac atggaaccca gcaacaagac cttaataaga aagaaagaag
     3121 agtaggaact cctcaaagta caaaaaagaa aaaagaaagc agaagagcca ctgaaagcag
     3181 aatacctgtt tcaaagagtc agccggtaac tcctgaaaaa catcgagcta gaaaaagaca
     3241 ggcatggctt tgggaagaag acaagaattt gagatctggc gtgaggaaat atggagaggg
     3301 aaactggtct aaaatactgt tgcattataa attcaacaac cggacaagtg tcatgttaaa
     3361 agacagatgg aggaccatga agaaactaaa actgatttcc tcagacagcg aagactgagg
     3421 atccaagggt gggcgcgccg acccagcttt cttgtacaaa gtggtgatga tccactagag
     3481 ttgtaggaat tcgccagcac agtggtcgac caattccggt tattttccac catattgccg
     3541 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3601 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3661 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3721 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     3781 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     3841 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     3901 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
     3961 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatacc
     4021 atgaaaacat ttaacatttc tcaacaggat ctagaattag tagaagtagc gacagagaag
     4081 attacaatgc tttatgagga taataaacat catgtgggag cggcaattcg tacgaaaaca
     4141 ggagaaatca tttcggcagt acatattgaa gcgtatatag gacgagtaac tgtttgtgca
     4201 gaagccattg cgattggtag tgcagtttcg aatggacaaa aggattttga cacgattgta
     4261 gctgttagac acccttattc tgacgaagta gatagaagta ttcgagtggt aagtccttgt
     4321 ggtatgtgta gggagttgat ttcagactat gcaccagatt gttttgtgtt aatagaaatg
     4381 aatggcaagt tagtcaaaac tacgattgaa gaactcattc cactcaaata tacccgaaat
     4441 taaaagtttt accataccaa gctcatcgat agatcctaat caacctctgg attacaaaat
     4501 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4561 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4621 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4681 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4741 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4801 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4861 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4921 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4981 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5041 gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     5101 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5161 aacgaagaca agatcacctg caggacaggc gcgccctgct ttttgcttgt actgggtctc
     5221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcacccggg
     5401 cgattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     5461 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5521 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5581 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5641 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5701 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5761 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5821 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5881 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5941 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6001 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6061 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6121 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6181 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6241 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6301 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6361 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6421 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6481 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6541 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6601 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6661 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6721 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6781 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6841 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6901 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6961 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7021 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7081 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7141 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7201 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7261 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7321 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7381 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7441 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     7501 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7561 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
     7621 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7681 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7741 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
     7801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7861 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     7921 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     7981 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     8041 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     8101 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     8161 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     8221 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     8281 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     8341 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     8401 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     8461 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8641 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8701 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8761 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8821 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8881 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8941 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     9001 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9061 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     9121 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     9181 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9241 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9301 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9361 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9421 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9481 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9541 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9601 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9661 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9721 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9781 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9841 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9901 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9961 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10021 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaat
//
