LOCUS       Exported               11897 bp DNA     circular SYN 23-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Dimitri Conomos
  TITLE     Direct Submission
  JOURNAL   Exported Feb 1, 2022 from SnapGene 5.3.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11897
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    19..136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        184..1362
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          415..1353
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             1417..2115
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             2140..3867
                     /codon_start=1
                     /label=hCoilin
                     /translation="MAASETVRLRLQFDYPPPATPHCTAFWLLVDLNRCRVVTDLISLI
                     RQRFGFSSGAFLGLYLEGGLLPPAESARLVRDNDCLRVKLEERGVAENSVVISNGDINL
                     SLRKAKKRAFQLEEGEETEPDCKYSKKHWKSRENNNNNEKVLDLEPKAVTDQTVSKKNK
                     RKNKATCGTVGDDNEEAKRKSPKKKEKCEYKKKAKNPKSPKVQAVKDWANQRCSSPKGS
                     ARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSK
                     EEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGF
                     LKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREE
                     NLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPK
                     KDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSS
                     LPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA
                     "
     3'UTR           3868..4710
                     /label=hCoilin 3'-UTR
     misc_feature    4774..5235
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             5240..6265
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    6300..6888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6771..6782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7036..7216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        7349..7453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7454..8314
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8485..9073
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9484..9505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9520..9550
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9558..9574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9582..9598
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9859..10492
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10539..10664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11161..11394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
       61 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      121 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      181 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      241 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      301 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      361 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      421 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      481 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      541 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      601 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      661 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      721 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      781 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      841 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      901 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      961 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1021 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1081 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1141 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1201 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1261 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1321 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccg cttaattaag
     1381 tttaaacgga tcccgccggc ggctagcgct accggtatga gcgagctgat taaggagaac
     1441 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     1501 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     1561 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     1621 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     1681 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     1741 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     1801 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     1861 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     1921 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     1981 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     2041 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     2101 gggcacaagc ttaataagta ctcagatcca ccaagcaaga tggcagcttc cgagacggtt
     2161 aggctacggc ttcaatttga ttacccgccg ccagctaccc cgcactgtac ggccttctgg
     2221 cttctggtcg acttgaacag atgccgagtc gtcacagatc tcattagtct catccgccag
     2281 cgcttcggct tcagttctgg ggccttccta ggcctctacc tggagggggg gctcttgccc
     2341 cccgccgaga gcgcgcgcct tgtgagagac aacgactgcc tcagagttaa attagaagag
     2401 agaggagttg ctgagaattc tgtagtcatc agtaatggtg acattaattt atctcttaga
     2461 aaagcaaaga agcgggcatt tcagttagag gagggtgaag aaactgaacc agattgcaaa
     2521 tattcaaaga agcattggaa gagtcgagag aacaataaca ataatgagaa ggtcttggat
     2581 ctggaaccaa aagctgtcac agatcagact gtcagcaaaa aaaacaagag aaaaaataaa
     2641 gcaacctgtg gcacagtggg tgatgataac gaagaggcca aaagaaaatc accaaagaaa
     2701 aaggagaaat gtgaatataa aaaaaaggct aagaatccca agtctccgaa agtacaggca
     2761 gtgaaagact gggccaatca gagatgtagt tctccaaaag gttctgctag aaacagcctt
     2821 gttaaagcca aaaggaaagg tagtgtaagc gtttgctcaa aagagagtcc cagttcctcc
     2881 tcggagtctg agtcttgtga tgaatctatc agtgatggtc ccagcaaagt cactttggag
     2941 gccagaaatt cctcagagaa attaccaact gagttatcaa aggaagaacc ctctaccaaa
     3001 aatacaactg cagacaaact ggctataaaa cttggcttta gccttacccc cagcaagggc
     3061 aagacctctg gaacaacatc ttccagttca gactctagtg cagagtcaga cgaccaatgc
     3121 ttgatgtcat cgagcacccc ggagtgtgct gcgggtttct taaagacagt aggccttttt
     3181 gcaggaagag gtcgtccagg cccagggctg tcatcacaga ctgcaggtgc tgctggatgg
     3241 aggcgttctg gctcaaatgg tggtggacag gctcctggtg cttctcccag tgtgtctctc
     3301 cctgctagtt taggaagagg atggggtaga gaagagaacc ttttttcttg gaagggagct
     3361 aagggacggg gcatgcgggg gagaggtcga ggacgagggc atcctgtttc ctgtgttgta
     3421 aatagaagca ctgacaacca gaggcaacag caattaaatg acgtggtaaa aaattcatct
     3481 actattatcc agaatccagt agagacaccc aagaaggact atagtctgtt accactgtta
     3541 gcagctgccc ctcaagttgg agaaaagatt gcatttaagc ttttggagct aacatccagt
     3601 tactctcctg atgtctctga ctacaaggaa ggaagaatat taagccacaa tccagagacc
     3661 cagcaagtag atatagaaat tctttcatcc ttacctgcct tgagagaacc tgggaaattt
     3721 gatttagttt atcacaatga aaatggagcc gaggtagtgg agtacgctgt gacacaggag
     3781 agcaagatca ctgtattttg gaaagagttg attgacccaa gactgattat tgaatctcca
     3841 agtaacacat caagtacaga acctgcctga gtatgacctc tccaccttat agtttatgaa
     3901 tgtcttgttt gtgaaagtga ctataaccca aacttttttt tttttttaaa gaggatttgg
     3961 aagttgtatg gatttttttg ttatcttcac tttactgcat aggaaacaat ctacctcatc
     4021 atttaaaatg acatgggtgt cggttttgta gatctttggt ttttttgtca ggtttaattt
     4081 cagttaacaa aatgtaaaac atgacattcc ctgcagatat tgttgtatac cagtatggtt
     4141 tcttctcttt ctttaaatgt ttttggccat caagtagcag tcgtcagtag gagtttataa
     4201 taccaagaat gtgctgcgta tcttgtctca ataagtttta agtaacattt aaaaatatta
     4261 aagcatgtta tttgacctaa ttttttagca tttgagttgt tccattaaat ggagcatctt
     4321 gtaaatttca agtattttat acttgcaatt gttaagagtt aacaggtagt tggatttgtc
     4381 gcagacaatg agttaaggaa tcctttcacg tttttcccaa ctttaaaatt aaggattctc
     4441 aggaccctgt gtagagcagt gaaaataaga tgtgcgtatg tgtgtgtatg cctggagaat
     4501 tggtgtttca cttcagtgag aggattggct gtgagcttca gaccaggaaa tgtgtcatct
     4561 tgccaggcac ctggctgagt gtgctggagt gaggatcttg aacagaaact tccttttctg
     4621 ttattattca ctacgaagct aaaatggcca aatatatacc gtgaaaattg gtttcattta
     4681 acaaaagatc agatccctcc ttcagctgta tctagagctc gaggtcgacc aattccggtt
     4741 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     4801 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     4861 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     4921 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     4981 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5041 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5101 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5161 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5221 aaaacacgat gataatacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     5281 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     5341 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     5401 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     5461 tccggaagtg cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg
     5521 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     5581 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     5641 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     5701 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     5761 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     5821 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     5881 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     5941 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     6001 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     6061 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     6121 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     6181 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     6241 cactcgtccg agggcaaagg aatagagtag atgccgaccg ggatctatcg atagatccta
     6301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6661 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     6901 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6961 ctggaagggc taattcactc ccaacgaaga caagatcacc tgcaggacag gcgcgccctg
     7021 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7081 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7141 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7201 tggaaaatct ctagcacccg ggcgattaag gaaagggcta gatcattctt gaagacgaaa
     7261 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7321 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7381 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7441 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7501 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7561 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7621 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7681 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     7741 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7801 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7861 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7921 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7981 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8041 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8101 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8161 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8221 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8281 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8341 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8401 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8461 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8521 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8581 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8641 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8701 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8761 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8821 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8881 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8941 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9001 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9061 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9241 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9301 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9421 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9481 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9541 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     9601 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9661 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     9721 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     9781 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     9841 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     9901 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     9961 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
    10021 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
    10081 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
    10141 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
    10201 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
    10261 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
    10321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    10501 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    10561 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    10621 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    10681 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    10741 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    10801 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    10861 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    10921 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    10981 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
    11041 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    11101 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    11161 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    11221 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    11281 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    11341 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    11401 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    11461 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    11521 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    11581 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    11641 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    11701 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    11761 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
    11821 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
    11881 tcgccgaatt cacaaat
//
