LOCUS       Exported               16494 bp DNA     circular SYN 17-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Michael Swan
  TITLE     Direct Submission
  JOURNAL   Exported Feb 1, 2022 from SnapGene 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..98
                     /label=GAGA
     primer_bind     99..118
                     /label=pbar-seq-f
                     228..2221
                     /note="note=""Fragment extracted from Tub67C Promoter+first
                     intron [1994  nt] : (AgeI[7] / KpnI[2005])"
     primer_bind     2221..2252
                     /label=MB2Fus_F1.FOR
     5'UTR           2227..2438
                     /label=bcd 5'UTR
     primer_bind     complement(2415..2438)
                     /label=pbs-bb-r
     primer_bind     complement(2415..2438)
                     /label=MB2Fus2_Vec.REV
     primer_bind     complement(2415..2438)
                     /label=MB4Fus_Vec.REV
     primer_bind     complement(2415..2438)
                     /label=MB4_Vec.REV
     primer_bind     complement(2416..2438)
                     /label=MB2Fus_F1.REV
     primer_bind     2424..2441
                     /label=MB11_pBS_swap.FOR
     misc_feature    2439..2546
                     /label=gblock-3xha
     misc_feature    2439..2441
                     /label=startcodon
     misc_feature    2442..2468
                     /label=ha
     misc_feature    2472..2498
                     /label=ha
     misc_feature    2505..2531
                     /label=ha
     primer_bind     2532..2556
                     /label=HA-Bcd-F
     misc_feature    2532..2546
                     /label=Linker
                     /note="note=""direct strand 
                     translation""translation=""EFDSAGSAGSAGGSS"""
     CDS             2547..4010
                     /codon_start=1
                     /label=Bicoid
                     /note="note=""direct strand 
                     translation""translation=""AQPPPDQNFYHHPLPHTHTHPHPHSHPHPHSH
                     PHPHHQHPQLQLPPQFRNPFDLLFDERTGAINYNYIRPYLPNQMPKPEELPDSLVMRRP
                     RRTRTTFTSSQIAELEQHFLQGRYLTAPRLADLSAKLALGTAQVKIWFKNRRRRHKIQS
                     DQHKDQSYEGMPLSPGMKQSDGDPPSLQTLSLGGGATPNALTPSPTPSTPTAHMTEHYS
                     "
                     /translation="AQPPPDQNFYHHPLPHTHTHPHPHSHPHPHSHPHPHHQHPQLQLP
                     PQFRNPFDLLFDERTGAINYNYIRPYLPNQMPKPEELPDSLVMRRPRRTRTTFTSSQIA
                     ELEQHFLQGRYLTAPRLADLSAKLALGTAQVKIWFKNRRRRHKIQSDQHKDQSYEGMPL
                     SPGMKQSDGDPPSLQTLSLGGGATPNALTPSPTPSTPTAHMTEHYSESFNAYYNYNGGH
                     NHAQANRHMHMQYPSGGGAGPGSTNVNGGQFFQQQQVHNHQQQLHHQGNHVPHQMQQQQ
                     QQAQQQQYHHFDFQQKQASACRVLVKDEPEADYNFNSSYYMRSGMSGATASASAVARGA
                     ASPGSEVYEPLTPKNDESPSLCGIGIGGPCAIAVGETEAADDMDDGTSKKTTLQILEPL
                     KGLDKSCDDGSSDDMSTGIRALAGTGNRGAAFAKFGKPSPPQGPQPPLGMGGVAMGESN
                     QYQCTMDTIMQAYNPHRNAAGNSQFAYCFN"
     primer_bind     2547..2579
                     /label=MB11_F1.FOR
     primer_bind     2547..2559
                     /label=MB11_pBS_swap.FOR
     misc_feature    2723..2740
                     /label=MB1SEQ3
     primer_bind     complement(2846..2867)
                     /label=M327.REV
     misc_feature    complement(3324..3341)
                     /label=MB1SEQ4
     CDS             4011..4490
                     /codon_start=1
                     /label=NLS_mCh_LEXY
                     /note="note=""direct strand 
                     translation""translation=""MAAAKRVKLDVSKGEEDNMAIIKEFMRFKVHM
                     EGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPA
                     DIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVM
                     QKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
                     N"
                     /translation="KGGSGGSGGSLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLT
                     EYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQ
                     PMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDELLKELADLNLD"
     misc_feature    4041..4448
                     /label=AsLOV2
                     /note="ugene_name=""AsLOV2"" - ugene_group=""CDS"" - 
                     1663..2070"
     misc_feature    4450..4487
                     /label=NES 21
                     /note="ugene_name=""NES 21"" - ugene_group=""CDS"" - 
                     2072..2109"
     primer_bind     complement(4466..4505)
                     /label=MB11_pBS_swap.REV
     primer_bind     complement(4467..4490)
                     /label=MB11_F1.REV
     primer_bind     4491..4550
                     /label=MB4Fus_Vec.FOR
     primer_bind     4491..4540
                     /label=MB4_Vec.FOR
     misc_feature    4491..4538
                     /label=FRT
     misc_feature    4539..5516
                     /label=bcd3'UTR
     misc_feature    5525..5730
                     /label=3xP3 hsp70
     misc_feature    5758..6435
                     /label=RFP
     misc_feature    6460..7272
                     /label=tubulin alpha 1 3'UTR
     primer_bind     7273..7321
                     /label=MB4Fus_Vec.FOR
     primer_bind     7273..7321
                     /label=MB4_Vec.FOR
     misc_feature    7273..7320
                     /label=FRT
     primer_bind     complement(7273..7287)
                     /label=MB11_pBS_swap.REV
     misc_feature    7327..7346
                     /label=GFP-Bcd F
     misc_feature    7350..8170
                     /label=sqh 3'UTR
     misc_feature    7470..7489
                     /label=GFP_RFP seq R
     misc_feature    8007..8026
                     /label=GFP-Bcd_out F
     primer_bind     complement(8149..8176)
                     /label=MB2Fus_F2.REV
     misc_feature    8171..8176
                     /label=SpeI site
     misc_feature    8177..8182
                     /label=pBABR homology
     misc_feature    8502..8752
                     /label=AttB
     misc_feature    complement(8784..9016)
                     /label=3' P-Element Sequence
     promoter        9855..9883
                     /label=Amp prom
     promoter        9855..9883
                     /label=Amp prom
     CDS             10123..10782
                     /label=AmpR
     misc_feature    10783..11779
                     /label=5' P-Element Sequence
     misc_feature    complement(12375..16494)
                     /label=mini-White+
     misc_feature    15423..15442
                     /label=mini w F
     misc_feature    15502..15521
                     /label=mini w R
ORIGIN
        1 aattggccgc tctagccccc cctcgaatgt tctctctctt ctcttctctc tctctttctc
       61 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctaag
      121 ggtcgagtcg atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt
      181 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaaccggtcc acggccgctt
      241 ggagggagat tagcaaaacg cagcgcgtga tcaaggtgac aatgaagcca ccgaccacca
      301 cggtggcgcc caacaaggca cgagtggttt gcatgtccga cacgcactcc ctgacgccct
      361 acatcaagtt cgatatacca gatggcgata tattcataca tgccggcgat tttaccaagt
      421 gcggccaact ggaggaggtg gaggagttta acacctggat tggagcactg ccccatcgcc
      481 acaagatcgt gattgctggc aatcatgagc tcagcttcga tagaaccttc acccatcctt
      541 tccagaagag caaaagtcat gcttcgagca gcaaacacac cggcatgtcc atcctcgatg
      601 atctgcccac tttgggcaac gccaaggaga acttggagag cgctgtgcaa acgcagaatg
      661 tccgcgatgt tctgaccaac tgtcggtatt tggaggatga actacttgag atttggggta
      721 ttcaaatata tggctcgcca tggcaaccgg aattctgtcg ctgggccttt aatgttccac
      781 gtggaactgc ctgtttggat aagtggaatc aaatcccgga aggcatcgat atcctggtca
      841 cccacacgcc gcccgtgggt cacggcgatc tctgctgttc cggcgtacga gccggttgtg
      901 tggagctact cagcacagtg cagcaacgag tgcgcccgaa ataccatgtc ttcggacatg
      961 tacacgaagg ctacggcatc accagcgatg gcaggatcat ctttgtgaat gcctcaacgt
     1021 gtgacatcaa ctacctaccc aacaatgccc ccatcgtttt cgatgtgacg ctgccccccg
     1081 gatttcgcaa ggattagcat gcctagatga ttatttattg tgtcacaggt tcaatgaatt
     1141 tattttcgtc ccactttatg tttatgttaa ggtatttcgt ctgtaaagat tttgaagcac
     1201 aattgtaggt gtctagagag atagaattca attcgcacga aattaaaaaa agaaattcac
     1261 acttaagatg ccataaaata tttattcaag tagagtgcat ttgtcaatat atgaatttgt
     1321 aaaaataaac aagttttgaa gacacctaaa cttttgaaga ttttcaatat gatattatat
     1381 agtttttaat aataatttaa gtaaaggtaa agcaaatcct gagatacata catactattt
     1441 caaattagta tcgataagcc gccagaaatg ggttggaaaa accttggaat gattattggt
     1501 tagcagccct gtctcccaat ttgacattcc tctttggcgc tctgtaacgg cgcttttcca
     1561 gtgactcccc aatcagcgaa ccaactaacc tgaggctgca cttttcgcgt ggcgaccaaa
     1621 aaaattataa aaaaaaggtc ccgcattaga ttttaatttg tatacaccca attttagtcc
     1681 agcaaaaaaa aactaatcaa ttggtgagaa gaaggaaaaa ctaattagtt taagattgct
     1741 gaaagcggga agtgaattta aatcagaaaa gatgtgtaag aagatgtgaa atgaaaaggt
     1801 gtgggaagtg aacatgaata aatgttcatt gaaaatgaac agctaattgc gaattttttg
     1861 aaacttaact gttaactgtt actgctcaca aatccttgaa aaatattaag atcgatctta
     1921 gaattgttta tttaaaatga attcctcgta tgtattataa cagacaatca agtttattaa
     1981 agaatattta aacttgtatt tttttttgag tttgataaaa tgtcccaaat ataaaagagt
     2041 attacctttt cgtcatataa tactgttctt aatattagtt ttagttagta atgtgtttat
     2101 aaagtaaatt acaaagggag gacctaacct aaccttaaac aatggcgcct tctcttgccc
     2161 agctcatcac ttttattgtt tcacccgcca gcgcgaagta gtgtccatcc agattgggac
     2221 ggtaccatct cttcgctcat ccctaaataa cggcactctg cagatgcgaa gcagtggatc
     2281 gcaaaaacgc aaaatgtggg cgaaataagt tcgcgagcgt ctcgaaagta tccggttact
     2341 gaaaatacaa gaaagtttcc acactccttt gccatttttc cgcgcggcgc ttggaaattc
     2401 gtaaagataa cgcggcggag tgtttggggc tagcaaagat gtacccatac gatgttcctg
     2461 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     2521 cagattacgc tgcgggaggt agtagtgcgc aaccgccgcc agatcaaaac ttttaccatc
     2581 atccgctgcc ccacacgcac acacatccgc atccgcactc ccatccgcat ccgcactcgc
     2641 atccgcaccc acatcaccaa catccgcagc ttcagttgcc gccacaattc cgaaatccct
     2701 tcgatttgct tttcgatgag cgaacgggag cgataaacta caactacata cgtccgtatc
     2761 tgcccaacca gatgcccaag ccagaggagc tgcccgactc tctggtgatg cggcgaccac
     2821 gtcgcacccg caccactttt accagctctc aaatagcaga gctggagcag cactttctgc
     2881 agggacgata cctcacagcc ccccgacttg cggatctgtc agcgaaacta gccctgggca
     2941 cagcccaggt gaagatatgg tttaagaacc gtcggcgtcg tcacaagatc caatcggatc
     3001 agcacaagga ccagtcctac gaggggatgc ctctctcgcc gggtatgaaa cagagcgatg
     3061 gcgatccccc cagcttgcag actcttagct tgggtggagg ggccacgccc aacgctttga
     3121 ctccgtcacc cacgccctca acgcccactg cacacatgac ggagcactac agcgagtcat
     3181 tcaacgccta ctacaactac aatggaggcc acaatcacgc ccaggccaat cgtcacatgc
     3241 acatgcagta tccttccgga gggggggcag gacctgggtc gaccaatgtc aatggcggcc
     3301 agttcttcca gcagcagcag gtccataatc accagcagca actgcaccac cagggcaacc
     3361 acgtgccgca ccagatgcag cagcagcaac agcaggctca gcagcagcaa taccatcact
     3421 ttgacttcca gcaaaagcaa gccagcgcct gtcgcgtcct ggtcaaggac gaaccggagg
     3481 ccgactacaa cttcaacagc tcgtactaca tgcgatcggg aatgtctggc gccactgcat
     3541 cggcatccgc tgtggcccga ggcgctgcct cgccgggctc cgaggtctac gagccattaa
     3601 cacccaagaa tgacgaaagt ccgagtctgt gtggcatcgg catcggcgga ccttgcgcca
     3661 tcgccgttgg cgagacggag gcggccgacg acatggacga cggaacgagc aagaagacga
     3721 cgctacagat cttggagcct ttgaagggtc tggacaagag ctgcgacgat ggcagtagcg
     3781 acgacatgag caccggaata agagccttag caggaaccgg aaatcgtgga gcggcatttg
     3841 ccaaatttgg caagccttcg cccccacaag gccctcagcc gcccctcggg atggggggcg
     3901 tggccatggg cgaatcgaac caatatcaat gcacgatgga tacgataatg caagcgtata
     3961 atccccatcg gaacgccgcg ggcaactcgc agtttgccta ctgcttcaat aagggtggat
     4021 ctggaggttc aggtggaagt ttggctacta cacttgaacg tattgagaag aactttgtca
     4081 ttactgaccc aagattgcca gataatccca ttatattcgc gtccgatagt ttcttgcagt
     4141 tgacagaata tagccgtgaa gaaattttgg gaagaaactg caggtttcta caaggtcctg
     4201 aaactgatcg cgcgacagtg agaaaaatta gagatgccat agataaccaa acagaggtca
     4261 ctgttcagct gattaattat acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc
     4321 agcctatgcg agatcagaag ggagatgtcc agtactttat tggggttcag ttggatggaa
     4381 ctgagcatgt ccgagatgct gccgagagag agggagtcat gctgattaag aaaactgcag
     4441 aaaatattga cgagctgctg aaggagctgg ccgacctgaa cctggactag gaagtaccta
     4501 ttccgaagtt cctattctct agaaagtata ggaacttccc tggatgagag gcgtgttaga
     4561 gagtttcatt agctttaggt taaccactgt tgttcctgat tgtacaaata ccaagtgatt
     4621 gtagatatct acgcgtagaa agttaggtct agtcctaaga tccgtgtaaa tggttcccag
     4681 ggaagtttta tgtactagcc tagtcagcag gccgcacgga ttccagtgca tatcttagtg
     4741 atactccagt taactctata ctttccctgc aatacgctat tcgccttaga tgtatctggg
     4801 tggctgctcc actgaagccc gggaatatgc aaccagttac atttgaggcc atttgggctt
     4861 aagcgtattc catggaaagt tatcgtccca catttcggaa attatattcc gagccagcaa
     4921 gaaaatcttc tctgttacaa tttgacatag ctaaaaactg tactaatcaa aatgaaaaat
     4981 gtttctcttg ggcgtaatct catacaatga ttacccttaa agatcgaaca tttaaacaat
     5041 aatatttgat atgatatttt caatttctat gctatgccaa agtgtctgac ataatcaaac
     5101 atttgcgcat tctttgacca agaatagtca gcaaattgta ttttcaatca atgcagacca
     5161 tttgtttcag attcggagat tttttgctgc caaacggaat aactatcata gctcacattc
     5221 taattacatc actaagaaga gcattgcaat ctgttaggcc tcaagtttaa ttttaaaatg
     5281 ctgcaccttt gatgttgtct ctttaagctt tgtattttta attacgaaaa tatataagaa
     5341 ctactctact cgggtaaatt gtgactaact acacataact acatacttag cccatatttc
     5401 cgtccctttc tagaatgaac gaaaacagta tctggttttc ccgaaaatct tatgaattta
     5461 aaaatgcact ttattgcaca tactcacaca tgcctgccat aaaatatgat tcgcgagcgg
     5521 ccgcggatct aattcaatta gagactaatt caattagagc taattcaatt aggatccaag
     5581 cttatcgatt tcgaaccctc gaccgccgga gtataaatag aggcgcttcg tctacggagc
     5641 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
     5701 caagcgcagc tgaacaagct aaacaatcgg gcggccgcac tagagccggt cgccaccatg
     5761 aggtcttcca agaatgttat caaggagttc atgaggttta aggttcgcat ggaaggaacg
     5821 gtcaatgggc acgagtttga aatagaaggc gaaggagagg ggaggccata cgaaggccac
     5881 aataccgtaa agcttaaggt aaccaagggg ggacctttgc catttgcttg ggatattttg
     5941 tcaccacaat ttcagtatgg aagcaaggta tatgtcaagc accctgccga cataccagac
     6001 tataaaaagc tgtcatttcc tgaaggattt aaatgggaaa gggtcatgaa ctttgaagac
     6061 ggtggcgtcg ttactgtaac ccaggattcc agtttgcagg atggctgttt catctacaag
     6121 gtcaagttca ttggcgtgaa ctttccttcc gatggacctg ttatgcaaaa gaagacaatg
     6181 ggctgggaag ccagcactga gcgtttgtat cctcgtgatg gcgtgttgaa aggagagatt
     6241 cataaggctc tgaagctgaa agacggtggt cattacctag ttgaattcaa aagtatttac
     6301 atggcaaaga agcctgtgca gctaccaggg tactactatg ttgactccaa actggatata
     6361 acaagccaca acgaagacta tacaatcgtt gagcagtatg aaagaaccga gggacgccac
     6421 catctgttcc tttagcggcc atcgaattcg agctcgccca ctaagcgtcg cgccacttca
     6481 acgctcgatg ggagcgtcat tggtgggcgg ggtaaccgtc gaaatcagtg tttacgcttc
     6541 caatcgcaac aaaaaattca ctgcaacact gaaaagcata cgaaaacgat gaagattgta
     6601 cgagaaacca taaagtattt tatccacaaa gacacgtata gcagaaaagc caagttaact
     6661 cggcgataag ttgtgtacac aagaataaaa tcggccagat tcagtgttgt cagaaataag
     6721 aaaaccccac tatgtttttc tttgcctttt ctttctccca gcgatcattc atttcgtggt
     6781 gaaagaacgg ggtcattgca cggagtttcg actgcgggaa agcagagctg ccgttcactt
     6841 cgtctataat tagcgctttc tattttcccc gattcgggcc gctgctgcgc ttttccgcct
     6901 gctgtttgtg gcaagtgtag cagcaggctg tgcacgcagt gtggcatgca cttggctttc
     6961 caccgttggt atcgattctc tgggacgatg agtcattcct ttcggggcca cagcataatc
     7021 gttgccagct caccgaaatg gtgacttcat ttcttaactg ccgtcaagca tgcgattgta
     7081 catacataca tatttatata tgtacatatt tatgtgacta tggtaggtcg atataatagc
     7141 aatcaacgca agcaaatgtg tcagtcctgc ttacaggaac gattctattt agtaattttc
     7201 gttgtataaa gtaattatgt atgtatgtaa gccccataaa tctgaaacaa ttaggcaaaa
     7261 ccatgcgaag ctgaagtacc tattccgaag ttcctattct ctagaaagta taggaacttc
     7321 catatggcgg ccgccattcg tagaattggg atccccagca gtcgattcac tagccagctg
     7381 cctctccaat cagcctgtcc aaactatcca tcatgagttt cgttttgata atttaatata
     7441 tacaagtata aatttttttt tttttggttt cgcgccttct gttctttgat tcctgataat
     7501 cctgtactta tccgtgcatt tgaattaaac aaaaagtagc caacaccagc gattgttatg
     7561 tacgacagtg tagtgtgtga gatgttggtg ccagataaca cgatttcccg aaaaactcct
     7621 ttcataagta ttaactcatt gaatatacat atatattacc tatatacaaa tacatactta
     7681 tacaagaatg tggcaataaa cctttgtttt tcttacactc gagaacgtgc gcacgtccaa
     7741 ccaaacagat gtctgcagaa atccttcagc caaaagcaac tgatcaaaca atagaaactt
     7801 tggaatatgt accccgcatg gctaactaag catgatcact agcatgatgt gataaaagta
     7861 tgtagcttca aatgtggagg gaaattgaaa gagatttcgg aaattagtca aaattttctt
     7921 ggaaccttcg acaagctgac taatgagttc atgtccaaaa gactcgaact tgtaaactca
     7981 tatcttatct cacattattt ttgattcgaa gatatttcag cttctgcaaa tatatgtttt
     8041 tcaagaattt atttaaaaaa aaaatcaatt aaatagatgg cttaatgttc gaaacactca
     8101 acgttgtata ttacacactt tttggacaac tattaattta aaagaaaaca cgaacatttg
     8161 aaaatctaga actagtggat ctggatccag actgcagaga agatctatag aattcagaga
     8221 ggccggcaga gaggctagca ggcatgcatc tagagtcgac ctcgaacgtt aacgttaacg
     8281 taacgttaac tcgaggagct tgataacatt atacctaaac ccatggtcaa gagtaaacat
     8341 ttctgccttt gaagttgaga acacaattaa gcatcccctg gttaaacctg cttgatagtg
     8401 caggctatac agaactgcgc tagtgactcc tatccgcctg cgtttctctg cctgctcacg
     8461 ggtcatatgc gtgctttgga gctgagggtg cggcggattg gcgtcgacga tgtaggtcac
     8521 ggcgggcatg tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt
     8581 gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg
     8641 gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc
     8701 gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cgacgatgta
     8761 ggtcacggcg ggcagccaag ctttgcgtac tcgcaaatta ttaaaaataa aactttaaaa
     8821 ataatttcgt ctaattaata ttatgagtta attcaaaccc cacggacatg ctaagggtta
     8881 atcaacaatc atatcgctgt ctcactcaga ctcaatacga cactcagaat actattcctt
     8941 tcactcgcac ttattgcaag catacgttaa gtggatgtct cttgccgacg ggaccacctt
     9001 atgttatttc atcatggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg
     9061 gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg
     9121 tttttttttt ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta
     9181 atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc
     9241 cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc
     9301 tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta
     9361 tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc
     9421 gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac
     9481 gaccttggag cgactgtcgt tagttccgcg cgattcgggt gcggtatttc acaccgcata
     9541 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     9601 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     9661 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     9721 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     9781 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9901 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    10141 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    10201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10441 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10801 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10861 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    11101 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    11161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    11221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11521 tcaggggggc ggagcctatg gaaaaacgcc ttcttcttga actcgggctc ggtgccagta
    11581 tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt
    11641 aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc
    11701 cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg
    11761 gactacgtgg gtctggccca tgatgaaata acataaggtg gtcccgtcga tagccgaagc
    11821 ttaccgaagt atacacttaa attcagtgca cgtttgcttg ttgagaggaa aggttgtgtg
    11881 cggacgaatt tttttttgaa aacattaacc cttacgtgga ataaaaaaaa atgaaatatt
    11941 gcaaattttg ctgcaaagct gtgactggag taaaattaat tcacgtgccg aagtgtgcta
    12001 ttaagagaaa attgtgggag cagagccttg ggtgcagcct tggtgaaaac tcccaaattt
    12061 gtgataccca ctttaatgat tcgcagtgga aggctgcacc tgcaaaaggt cagacattta
    12121 aaaggaggcg actcaacgca gatgccgtac ctagtaaagt gatagagcct gaaccagaaa
    12181 agataaaaga aggctatacc agtgggagta cacaaacaga gtaagtttga atagtaaaaa
    12241 aaatcattta tgtaaacaat aacgtgactg tgcgttaggt cctgttcatt gtttaatgaa
    12301 aataagagct tgagggaaaa aattcgtact ttggagtacg aaatgcgtcg tttagagcag
    12361 cagccgaatt aattctagtt ccagtgaaat ccaagcattt tctaaattaa atgtattctt
    12421 attattatag ttgttatttt tgatatatat aaacaacact attatgccca ccattttttt
    12481 gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa ttgtaacgct
    12541 aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta aattagaata
    12601 aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg tacatacaaa
    12661 tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt gagcgaaccg
    12721 aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc
    12781 ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat
    12841 tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat
    12901 gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag
    12961 acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc
    13021 atatcttgtt tttattggca caaatataat tacattagct ttttgagggg gcaataaaca
    13081 gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc
    13141 ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc
    13201 acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc
    13261 gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt
    13321 tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac
    13381 catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag
    13441 ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc catcgaggtc
    13501 gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc caccagagtg
    13561 accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat catcggatag
    13621 gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc aatcgttttg
    13681 cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat aaaaactggc
    13741 agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa attattgaaa
    13801 tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt gaaaggtcat
    13861 gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt gcgtgagttg
    13921 ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa aatgttactc
    13981 gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa aactataaac
    14041 tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct atctaaaatc
    14101 tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact ggaaccactc
    14161 accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac cgacagccag
    14221 gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt gtacccattc
    14281 tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc catatcccgg
    14341 gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg ggactcgatc
    14401 tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt gtagttggta
    14461 ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta gataccctta
    14521 ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag tgcccaagaa
    14581 agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca gctcggaaga
    14641 cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct tcagcacctg
    14701 gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc agatcagaag
    14761 cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc cgccggacag
    14821 acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga gcgaaagctc
    14881 ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc gtggcatccg
    14941 caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa agaggtcatc
    15001 ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt ggccattgag
    15061 cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg caagggcatt
    15121 cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt cgcccggata
    15181 ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag atttcacaca
    15241 gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct gggcgccggt
    15301 atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg ccatccggag
    15361 cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt gaggttctcg
    15421 gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg gagcgtgccg
    15481 tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc tccgctgtca
    15541 ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat ctaaccccga
    15601 acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta ttttttgtgc
    15661 atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga atatagtcga
    15721 aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc ttatatagcg
    15781 agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat tgcggtttgg
    15841 gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat cgcagttcct
    15901 atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc agatgctcgg
    15961 cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg cccattgccg
    16021 ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc caatcactca
    16081 aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt gtttaattga
    16141 tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac tgaaggcgga
    16201 cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa
    16261 gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt tgagtggaat
    16321 gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct tggatcgaag
    16381 taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt
    16441 tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata ctag
//
