LOCUS       pSEM371___unc_11        4088 bp    DNA        circular     02-FEB-2022
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Unpublished reagents. Sonia el Mouridi and Christian
            Froekjaer-Jensen. Synthetic Genome Biology lab. KAUST.
COMMENT     www.wormbuilder.org
COMMENT     MOdular Safe harbor Transgene Insertion (mosTI). 
COMMENT     Transgene fragment for array insertion into ce-unc-119 locus. Use
            with pSEM376 (sgRNA spacer: TTTGGGAACCAGGTGTTGG)
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(1124..1241,1293..1430,1482..1748)
                     /locus_tag="recoded unc-119 (non-rescuing)"
                     /label="recoded unc-119 (non-rescuing)"
                     /ApEinfo_label="recoded unc-119 (non-rescuing)"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(843..861)
                     /locus_tag="pSEM376 spacer"
                     /label="pSEM376 spacer"
                     /ApEinfo_label="pSEM376 spacer"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1868..1888
                     /locus_tag="Uni_9 R"
                     /label="Uni_9 R"
                     /ApEinfo_label="Uni_9 R"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(845..846)
                     /locus_tag="Cut between TT"
                     /label="Cut between TT"
                     /ApEinfo_label="Cut between TT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(2941..3801)
                     /codon_start="1"
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1050..1123
                     /locus_tag="recoded unc-119 (non-rescuing)(1)"
                     /label="recoded unc-119 (non-rescuing)(1)"
                     /ApEinfo_label="recoded unc-119 (non-rescuing)"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1924..1940)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(3802..3906)
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1050..1052
                     /locus_tag="Met - > Ile to prevent rescue"
                     /label="Met - > Ile to prevent rescue"
                     /ApEinfo_label="Met - > Ile to prevent rescue"
                     /ApEinfo_fwdcolor="#d783ff"
                     /ApEinfo_revcolor="#d783ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(2112..2700)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4020..4036
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="#fc6665"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(2817..2841)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4067..4088
                     /locus_tag="Uni_9 F"
                     /label="Uni_9 F"
                     /ApEinfo_label="Uni_9 F"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           1749..1861
                     /locus_tag="ce-eft-3 3'UTR"
                     /label="ce-eft-3 3'UTR"
                     /ApEinfo_label="ce-eft-3 3'UTR"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 tctagatgag agagagagcg tgtttatgca cgcagtatac taactcctga tatccaattt
       61 catatatcga tatgcttgct ttcttttctt ctacgttgaa attgtttgaa cttttctcaa
      121 aaaacgcgga ttttagagag acttagagtt gctccaaata aatttaattt gcaattttgc
      181 acttactatt agaatctact tttatagcgc taaaatgacc gaataacaca gtagatttta
      241 aaagaaaatt ttaaaaatta tttttttgtg cagtcaaatt tcgctagttt ttgccactca
      301 tttttgaaca tttgtatatg tgcctttcgg tggttcaatc aggggtggca cgtggtgtca
      361 gagtgttcca tttcggtttg atctacgttg atctacaaaa aatgcgggag aagagacgca
      421 gagttctcaa ctgatttcac atggttatga accaggggtg ttcggcaaat ttgccgtttg
      481 ccgagctggg caaattttga gatttgccgc acaccccttt tatgaacgtg ctgacgtcac
      541 atctttttgg acaaagaatt cccgcatttt ttgtatatca aaccgtgatg ggacagcctg
      601 gcaccacgtg gggttggcgg caaatgattt tttccgacaa atcggcaaat tggcaaattg
      661 ccggaattta aagtttctgg caaattgttg atttgccgaa aaaaaatttc aaaaaggcac
      721 agttttaagt gtttccgtct tataaaatat ttctctaaaa atttccggaa aacggaaaat
      781 tgccggaaat acaaagtttc gtcaaatcgg caaacctgca atttgccgaa cggcaactgc
      841 cgccaacacc tggttcccaa aaaagcgtgc actgtttgaa tattaaaaaa aattcacaca
      901 aaaataataa attgaaatag gctaaattca catctatgta caaaaattgc atgccagcac
      961 cggtcaattt tctcaattac tttttgtcta tttttgccga acataatttg ctgtttcctt
     1021 ggcagaatac agaaaactct ttttttcagA TCCCACGTCC ACCTCCAAGT ACAGAACAAG
     1081 GTATCACCAC CGAATCCGAA CTGGCCAAAA AAGCGCAGAT CACCCCGAAC GACGTGCTGG
     1141 CCCTGCCAGG CATTACCCAA GGCTTCCTGT GCAGCCCAAG CGCGAACATT TATAACATTG
     1201 AGTTTACCAA GTTTCAGATC CGCGATCTGG ATACCGAGCA Ggtaagttta aacatatata
     1261 tactaactaa ccctgattat ttaaattttc agGTGCTGTT TGAGATTGCG AAGCCGGAAA
     1321 ACGACCAGGA GAACGATGAA TCCCCGCAAG AAAGCGCCCG CTACGTGCGC TACCGCTTTG
     1381 CGCCGAACTT TCTCAAACTC AAAACCGTGG GAGCGACCGT CGAATTTAAG gtaagtttaa
     1441 acagttcggt actaactaac catacatatt taaattttca gGTGGGCGAT ATTCCAATTC
     1501 ATCATTTTCG TATGATTGAA CGCCATTTCT TTAAAGATCG CCTCCTGAAG TGCTTTGATT
     1561 TCGAATTTGG ATTTTGCATT CCAAACAGCC GTAACAACTG CGAACATATC TATGAGTTTC
     1621 CGCAACTGAG CCAGCAGCTC ATGGATGATA TGATCAACAA CCCGAACGAA ACCCGTAGCG
     1681 ACAGCTTTTA CTTTGTCGAT AACAAACTCG TGATGCACAA CAAAGCGGAC TATAGCTACG
     1741 ATGCGTAAat cttcattgtt gagtttatct tgttgatttt tgaataaatt atcaactctt
     1801 tactttttaa tgggttatga aataaataaa cattgaaaac tgataaacaa cgttcatctc
     1861 ttctagaagg ctaggtggag gctcagtgat gataagtctg cgatggtgga tgcatgtgtc
     1921 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc agagggcaca atcctattcc
     1981 gcgctatccg acaatctcca agacattagg tggagttcag ttcggcgtat ggcatatgtc
     2041 gctggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2701 gaagatcctt tgatcttttc tacggggtct gacgctctat tcaacaaagc cgccgtcccg
     2761 tcaagtcagc gtaaatgggt agggggcttc aaatcgtcct cgtgatacca attcggagcc
     2821 tgcttttttg tacaaacttg ttgataatgg caattcaagg atcttcacct agatcctttt
     2881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3061 cagtgctgca atgataccgc gagagccacg ctcaccggct ccagatttat cagcaataaa
     3121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3901 tccgcgcaca tttccccgaa aagtgccaga tacctgaaac aaaacccatc gtacggccaa
     3961 ggaagtctcc aataactgtg atccaccaca agcgccaggg ttttcccagt cacgacgttg
     4021 taaaacgacg gccagtcatg cataatccgc acgcatctgg aataaggaag tgccattccg
     4081 cctgacct     
//
