LOCUS       413_pCMV_NfL-FLAG        5636 bp    DNA     circular     31-12-1969
ACCESSION   413_pCMV_NfL-FLAG
VERSION     413_pCMV_NfL-FLAG.0
KEYWORDS    .
COMMENT     VNTNAME|413_pCMV_NfL-FLAG|
            VNTDATE|-13325325|
            VNTDBDATE|-14092722|
            LOCALIND|1|
FEATURES             Location/Qualifiers
     misc_feature    1685..1708
                     /vntifkey="21"
                     /label=FLAG
     misc_feature    41..1669
                     /vntifkey="21"
                     /label=NFL
     rep_origin      4327..4946
                     /vntifkey="33"
                     /label=pBR322_origin
     terminator      3910..4179
                     /vntifkey="43"
                     /label=TK_PA_terminator
     gene            2944..3732
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS$CDS_pept    2941..3735
                     /gene="NeoR/KanR"
                     /vntifkey="4"
                     /label=NeoR/KanR
                     /note="ORF frame 1"
     misc_feature    2819..2838
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     rep_origin      2757..2834
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        2590..2858
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    complement(6536..6751)
                     /vntifkey="21"
                     /label=SV40_enhancer
     misc_feature    complement(6530..6550)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     promoter        2478..2506
                     /vntifkey="30"
                     /label=AmpR_promoter
     rep_origin      complement(6051..6357)
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    1924..1943
                     /vntifkey="21"
                     /label=EBV_rev_primer
     promoter        5565..5634
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    5564..5584
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        5055..5607
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    5110..5397
                     /vntifkey="21"
                     /label=CAG_enhancer
     primer_bind     927..1856
                     /vntifkey="28"
                     /label=sequence confirmed
     misc_feature    1670..1684
                     /vntifkey="21"
                     /label=linker
     misc_feature    1364..1423
                     /vntifkey="21"
                     /label=DA2 antibody recognizing epitope
ORIGIN
        1 ctagcgctac cggactcaga tctcgagctc aagcttcacc atgagttcgt tcggctacga
       61 tccgtacttt tcgacctcct acaagcggcg ctatgtggag acgccccggg tgcacatctc
      121 cagcgtgcgc agcggctaca gcacggcgcg ctccgcgtac tccagctact ccgcgccggt
      181 ctcctcctcg ctgtccgtgc gccgcagcta ctcgtccagc tctggctctt tgatgcccag
      241 cctggagaat ctcgatctga gccaggtagc cgccatcagc aacgacctca agtctatccg
      301 cacacaagag aaggcacagc tgcaggacct caacgatcgc ttcgccagct tcatcgagcg
      361 cgtgcacgag ctggagcagc agaacaaggt cctggaagcc gagctgttgg tgctgcgcca
      421 gaaacactct gagccttccc gcttccgcgc cctgtacgag caggagatcc gcgatctgcg
      481 gctggcagcg gaagacgcca ctaacgagaa gcaggcgctg cagggcgagc gcgaggggct
      541 ggaggagact ctgcgcaacc tgcaggctcg ctatgaggaa gaagtgctga gccgcgagga
      601 cgccgagggc cggctgatgg aagcgcgcaa aggtgcggat gaggccgcgc tcgcccgcgc
      661 cgagctggag aagcgcatcg acagcctgat ggacgagata gctttcctga agaaggtgca
      721 cgaggaagag atcgccgagc tgcaggctca gatccagtat gctcagatct ccgtggagat
      781 ggacgtgtcc tccaagcccg acctctccgc cgctctcaag gacatccgcg ctcagtacga
      841 gaagctggcc gccaagaaca tgcagaacgc cgaagagtgg ttcaagagcc gcttcaccgt
      901 gctaaccgag agcgccgcca agaacaccga cgctgtgcgc gctgccaagg acgaggtgtc
      961 ggaaagccgc cgcctgctca aggctaagac cctggagatc gaagcctgcc ggggtatgaa
     1021 cgaagctctg gagaagcagc tgcaggagct agaggacaag cagaatgcag acattagcgc
     1081 catgcaggac acaatcaaca aactggagaa tgagctgaga agcacgaaga gcgagatggc
     1141 caggtacctg aaggagtacc aggacctcct caatgtcaag atggccttgg acatcgagat
     1201 tgcagcttac agaaaactct tggaaggcga agagaccagg ctcagtttca ccagcgtggg
     1261 tagcataacc agcggctact ctcagagctc gcaggtcttc ggccgttctg cttacagtgg
     1321 cttgcagagc agctcctact tgatgtctgc tcgctctttc ccagcctact ataccagcca
     1381 cgtccaggaa gagcagacag aggtcgagga gaccattgag gctacgaaag ctgaggaggc
     1441 caaggatgag cccccctctg aaggagaagc agaagaggag gagaaggaga aagaggaggg
     1501 agaggaagag gaaggcgctg aggaggaaga agctgccaag gatgagtctg aagacacaaa
     1561 agaagaagaa gaaggtggtg agggtgagga ggaagacacc aaagaatctg aagaggaaga
     1621 gaagaaagag gagagtgctg gagaggagca ggtggctaag aagaaagatg gggcccggga
     1681 tccggactac aaagacgatg acgacaagtg agcggccgcg actctagatc ataatcagcc
     1741 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1801 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1861 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1921 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     1981 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2041 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2101 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2161 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2221 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2281 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2341 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2401 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2461 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2521 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     2581 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2641 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2701 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2761 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2821 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     2881 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     2941 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3001 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3061 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3121 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3181 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3241 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3301 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3361 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3421 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     3481 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3541 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3601 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3661 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3721 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     3781 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     3841 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     3901 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     3961 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4021 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4081 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4141 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     4201 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4261 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4741 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4981 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     5041 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5101 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5161 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5221 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5281 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5341 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5401 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     5461 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5521 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     5581 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccg
//
