LOCUS       414_pCMV_NfL(K211TAG)-FLAG        5636 bp    DNA     circular
            31-12-1969
ACCESSION   414_pCMV_NfL(K211TAG)-FLAG
VERSION     414_pCMV_NfL(K211TAG)-FLAG.0
KEYWORDS    .
COMMENT     VNTNAME|414_pCMV_NfL(K211TAG)-FLAG|
            VNTDATE|-13325327|
            VNTDBDATE|-13345341|
            LOCALIND|1|
FEATURES             Location/Qualifiers
     misc_feature    1686..1709
                     /vntifkey="21"
                     /label=FLAG
     primer_bind     672..674
                     /vntifkey="28"
                     /label=K211TAG
     misc_feature    42..1670
                     /vntifkey="21"
                     /label=NFL
     rep_origin      4328..4947
                     /vntifkey="33"
                     /label=pBR322_origin
     terminator      3911..4180
                     /vntifkey="43"
                     /label=TK_PA_terminator
     gene            2945..3733
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS$CDS_pept    2942..3736
                     /gene="NeoR/KanR"
                     /vntifkey="4"
                     /label=NeoR/KanR
                     /note="ORF frame 1"
     misc_feature    2820..2839
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     rep_origin      2758..2835
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        2591..2859
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    complement(6536..6751)
                     /vntifkey="21"
                     /label=SV40_enhancer
     misc_feature    complement(6530..6550)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     promoter        2479..2507
                     /vntifkey="30"
                     /label=AmpR_promoter
     rep_origin      complement(6051..6357)
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    1925..1944
                     /vntifkey="21"
                     /label=EBV_rev_primer
     promoter        5566..5635
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    5565..5585
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        5056..5608
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    5111..5398
                     /vntifkey="21"
                     /label=CAG_enhancer
     primer_bind     976..1908
                     /vntifkey="28"
                     /label=sequence confirmed
     primer_bind     1..768
                     /vntifkey="28"
                     /label=sequence confirmed
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcac catgagttcg ttcggctacg
       61 atccgtactt ttcgacctcc tacaagcggc gctatgtgga gacgccccgg gtgcacatct
      121 ccagcgtgcg cagcggctac agcacggcgc gctccgcgta ctccagctac tccgcgccgg
      181 tctcctcctc gctgtccgtg cgccgcagct actcgtccag ctctggctct ttgatgccca
      241 gcctggagaa tctcgatctg agccaggtag ccgccatcag caacgacctc aagtctatcc
      301 gcacacaaga gaaggcacag ctgcaggacc tcaacgatcg cttcgccagc ttcatcgagc
      361 gcgtgcacga gctggagcag cagaacaagg tcctggaagc cgagctgttg gtgctgcgcc
      421 agaaacactc tgagccttcc cgcttccgcg ccctgtacga gcaggagatc cgcgatctgc
      481 ggctggcagc ggaagacgcc actaacgaga agcaggcgct gcagggcgag cgcgaggggc
      541 tggaggagac tctgcgcaac ctgcaggctc gctatgagga agaagtgctg agccgcgagg
      601 acgccgaggg ccggctgatg gaagcgcgca aaggtgcgga tgaggccgcg ctcgcccgcg
      661 ccgagctgga gtagcgcatc gacagcctga tggacgagat agctttcctg aagaaggtgc
      721 acgaggaaga gatcgccgag ctgcaggctc agatccagta tgctcagatc tccgtggaga
      781 tggacgtgtc ctccaagccc gacctctccg ccgctctcaa ggacatccgc gctcagtacg
      841 agaagctggc cgccaagaac atgcagaacg ccgaagagtg gttcaagagc cgcttcaccg
      901 tgctaaccga gagcgccgcc aagaacaccg acgctgtgcg cgctgccaag gacgaggtgt
      961 cggaaagccg ccgcctgctc aaggctaaga ccctggagat cgaagcctgc cggggtatga
     1021 acgaagctct ggagaagcag ctgcaggagc tagaggacaa gcagaatgca gacattagcg
     1081 ccatgcagga cacaatcaac aaactggaga atgagctgag aagcacgaag agcgagatgg
     1141 ccaggtacct gaaggagtac caggacctcc tcaatgtcaa gatggccttg gacatcgaga
     1201 ttgcagctta cagaaaactc ttggaaggcg aagagaccag gctcagtttc accagcgtgg
     1261 gtagcataac cagcggctac tctcagagct cgcaggtctt cggccgttct gcttacagtg
     1321 gcttgcagag cagctcctac ttgatgtctg ctcgctcttt cccagcctac tataccagcc
     1381 acgtccagga agagcagaca gaggtcgagg agaccattga ggctacgaaa gctgaggagg
     1441 ccaaggatga gcccccctct gaaggagaag cagaagagga ggagaaggag aaagaggagg
     1501 gagaggaaga ggaaggcgct gaggaggaag aagctgccaa ggatgagtct gaagacacaa
     1561 aagaagaaga agaaggtggt gagggtgagg aggaagacac caaagaatct gaagaggaag
     1621 agaagaaaga ggagagtgct ggagaggagc aggtggctaa gaagaaagat ggggcccggg
     1681 atccggacta caaagacgat gacgacaagt gagcggccgc gactctagat cataatcagc
     1741 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1801 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1921 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     1981 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2041 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2101 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2161 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2221 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2281 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2341 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2401 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2461 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2521 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     2581 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2641 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     2701 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2821 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2881 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     2941 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3001 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3061 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3121 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3181 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3241 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3301 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3361 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3421 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     3481 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3541 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3601 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3661 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3721 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     3781 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3841 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     3901 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     3961 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4021 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     4081 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     4141 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     4201 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4261 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4321 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4381 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4441 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4501 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4561 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4621 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4681 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4741 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4801 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4861 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4921 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4981 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5041 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5101 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     5161 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5221 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5281 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5341 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5401 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     5461 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     5521 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     5581 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatcc
//
