LOCUS       641_pCMV_FLAG-NFL(R438TAG)        5621 bp    DNA     circular
            31-12-1969
ACCESSION   641_pCMV_FLAG-NFL(R438TAG)
VERSION     641_pCMV_FLAG-NFL(R438TAG).0
KEYWORDS    .
COMMENT     VNTNAME|641_pCMV_FLAG-NFL(R438TAG)|
            VNTDATE|-14082549|
            VNTDBDATE|-14082600|
            LOCALIND|1|
FEATURES             Location/Qualifiers
     misc_feature    68..1693
                     /vntifkey="21"
                     /label=NFL R438
     misc_feature    5095..5382
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        5040..5592
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    5549..5569
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        5550..5619
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    1909..1928
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(6051..6357)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        2463..2491
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(6530..6550)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(6536..6751)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2575..2843
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2742..2819
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2804..2823
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS$CDS_pept    2926..3720
                     /gene="NeoR/KanR"
                     /vntifkey="4"
                     /label=NeoR/KanR
                     /note="ORF frame 1"
     gene            2929..3717
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     terminator      3895..4164
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      4312..4931
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    44..67
                     /vntifkey="21"
                     /label=FLAG
     primer_bind     6..1764
                     /vntifkey="28"
                     /label=20210914_sequence confirmed
ORIGIN
        1 ctagcgctac cggactcaga tctcgagctc aagcttcacc atggactaca aagacgatga
       61 cgacaagagt tcgttcggct acgatccgta cttttcgacc tcctacaagc ggcgctatgt
      121 ggagacgccc cgggtgcaca tctccagcgt gcgcagcggc tacagcacgg cgcgctccgc
      181 gtactccagc tactccgcgc cggtctcctc ctcgctgtcc gtgcgccgca gctactcgtc
      241 cagctctggc tctttgatgc ccagcctgga gaatctcgat ctgagccagg tagccgccat
      301 cagcaacgac ctcaagtcta tccgcacaca agagaaggca cagctgcagg acctcaacga
      361 tcgcttcgcc agcttcatcg agcgcgtgca cgagctggag cagcagaaca aggtcctgga
      421 agccgagctg ttggtgctgc gccagaaaca ctctgagcct tcccgcttcc gcgccctgta
      481 cgagcaggag atccgcgatc tgcggctggc agcggaagac gccactaacg agaagcaggc
      541 gctgcagggc gagcgcgagg ggctggagga gactctgcgc aacctgcagg ctcgctatga
      601 ggaagaagtg ctgagccgcg aggacgccga gggccggctg atggaagcgc gcaaaggtgc
      661 ggatgaggcc gcgctcgccc gcgccgagct ggagaagcgc atcgacagcc tgatggacga
      721 gatagctttc ctgaagaagg tgcacgagga agagatcgcc gagctgcagg ctcagatcca
      781 gtatgctcag atctccgtgg agatggacgt gtcctccaag cccgacctct ccgccgctct
      841 caaggacatc cgcgctcagt acgagaagct ggccgccaag aacatgcaga acgccgaaga
      901 gtggttcaag agccgcttca ccgtgctaac cgagagcgcc gccaagaaca ccgacgctgt
      961 gcgcgctgcc aaggacgagg tgtcggaaag ccgccgcctg ctcaaggcta agaccctgga
     1021 gatcgaagcc tgccggggta tgaacgaagc tctggagaag cagctgcagg agctagagga
     1081 caagcagaat gcagacatta gcgccatgca ggacacaatc aacaaactgg agaatgagct
     1141 gagaagcacg aagagcgaga tggccaggta cctgaaggag taccaggacc tcctcaatgt
     1201 caagatggcc ttggacatcg agattgcagc ttacagaaaa ctcttggaag gcgaagagac
     1261 caggctcagt ttcaccagcg tgggtagcat aaccagcggc tactctcaga gctcgcaggt
     1321 cttcggccgt tctgcttaca gtggcttgca gagcagctcc tacttgatgt ctgcttagtc
     1381 tttcccagcc tactatacca gccacgtcca ggaagagcag acagaggtcg aggagaccat
     1441 tgaggctacg aaagctgagg aggccaagga tgagcccccc tctgaaggag aagcagaaga
     1501 ggaggagaag gagaaagagg agggagagga agaggaaggc gctgaggagg aagaagctgc
     1561 caaggatgag tctgaagaca caaaagaaga agaagaaggt ggtgagggtg aggaggaaga
     1621 caccaaagaa tctgaagagg aagagaagaa agaggagagt gctggagagg agcaggtggc
     1681 taagaagaaa gattgagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     1741 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1801 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1921 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     1981 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2041 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2101 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2161 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2221 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2281 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2341 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2401 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2521 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     2581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2641 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2881 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2941 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3001 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3061 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3121 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3181 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3241 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3301 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3361 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3421 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3481 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3541 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3601 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3661 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3721 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     3781 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     3841 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     3901 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     3961 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4021 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4081 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4141 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     4201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     5041 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     5101 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     5161 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5221 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5281 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5341 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     5401 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     5461 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     5521 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     5581 gtctatataa gcagagctgg tttagtgaac cgtcagatcc g
//
