LOCUS       Exported               10601 bp ds-DNA     circular SYN 03-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pL-GW-EcoDam-V5-mseLMNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 3, 2022 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2398..2878
                     /label=HSP
     misc_feature    2885..2890
                     /label=Kozak
     misc_feature    2891..3724
                     /label=EcoDam
     CDS             2894..3587
                     /codon_start=1
                     /label=EcoDam
                     /translation="KKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTD
                     FSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPF
                     RRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCES
                     YADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPV
                     LISNHDTML"
     CDS             3731..3772
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3731..3772
                     /label=V5-tag
     protein_bind    3788..3812
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3829..3832
                     /label=CACC
     misc_feature    3833..5830
                     /label=LaminA(Mm)
     misc_feature    5847..5863
                     /label=core
     misc_recomb     5864..5871
                     /label=attR2
     misc_recomb     5864..5871
                     /label=retained from attR2
     LTR             6402..6582
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6614..6838
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6884..7312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7326..7656
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7507..7642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7704..7751
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7770..8144
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8274..8395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8444..8460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8468..8484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8492..8522)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8537..8558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8846..9434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9605..10465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10466..10570)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcccccgg gctgcaggaa
     2401 ttcagatctc ggccgcatat taagtgcatt gttctcgata ccgctaagtg cattgttctc
     2461 gttagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg
     2521 ttctcttgct gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcagtaccc
     2581 gggagtaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
     2641 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
     2701 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
     2761 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
     2821 caagaagaga actctgaata ctttcaacaa gttaccgaga aagaagaact cacacacagc
     2881 tagcgccacc atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct
     2941 gcttgatgat attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg
     3001 tgccgggtcg gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag
     3061 cgacctgatc agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc
     3121 acgcgagctg tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga
     3181 gttcaacaaa agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta
     3241 cggttacaac ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgttcggccg
     3301 ctacaaaaaa ccctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa
     3361 tgcctttttc tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt
     3421 cgtctattgc gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca
     3481 cacaaacagt tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt
     3541 tgagcgccat attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta
     3601 tcagcgcgca aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac
     3661 acgtaaaaag gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa
     3721 aaaaaccggt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgatcgattt
     3781 cgatcaccca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca ccatggagac
     3841 cccgtcacag cggcgcgcca cccgcagtgg ggcgcaggcc agctctaccc cactgtcgcc
     3901 cactcggatc acccggctgc aggagaagga ggacctgcag gagctcaatg accgcctggc
     3961 cgtgtacatc gatcgcgtgc gttccctgga gaccgagaac gcggggctgc gccttcgcat
     4021 cactgagtct gaagaggtgg tcagccgaga ggtgtccggc atcaaggcgg cctacgaggc
     4081 cgagctgggg gatgcccgca agacccttga ttctgtggcc aaggagcgcg cccgcctcca
     4141 gctagagctg agcaaagtgc gtgaggagtt caaggagctg aaggctcgca acaccaagaa
     4201 ggagggggac ttgttggctg cgcaggcccg gctcaaggac ctcgaggctc ttctcaactc
     4261 caaggaagct gccctgagca ctgctctcag tgagaagcgc acattggagg gcgagctcca
     4321 tgacctgcgg gggcaggtag ccaagcttga ggcggccctg ggagaggcta agaagcagct
     4381 tcaggatgag atgctgaggc gagtggatgc tgagaacagg ctacagacgc tgaaggagga
     4441 gcttgacttc cagaagaaca tttacagcga ggaactgcgt gagaccaagc gccggcatga
     4501 gacgcggctt gtggagatcg ataacgggaa gcagcgagag tttgagagcc ggctggcaga
     4561 tgccctgcag gagctgcggg ctcagcatga ggaccaggtg gaacagtata agaaggagct
     4621 agaaaagaca tactccgcca agctggataa tgccaggcag tctgctgaga ggaacagcaa
     4681 cctcgtgggg gctgcccatg aggaactgca gcagtctcga atccgcattg acagcctctc
     4741 ggcccagctc agccagctcc aaaagcagtt ggcagccaag gaggcaaagc tgcgtgacct
     4801 ggaggactcg ctggcccgtg agcgcgatac cagccggcgc ctgctggctg agaaagagcg
     4861 agagatggcg gagatgcggg cgaggatgca gcagcagctg gacgagtacc aggagctgct
     4921 ggacatcaag ctggccctgg acatggagat ccatgcctac cgaaagctgc tggagggcga
     4981 ggaggagagg ctgcgcctgt cccccagccc tacctcgcag cgcagccgtg gccgcgcctc
     5041 ctcccactca tcccagtctc agggtggagg cagcgtcacc aaaaagcgca agctggagtc
     5101 ttccgagagc cggagcagct tctcgcagca tgctcgcact agcgggcgtg tggcggtaga
     5161 ggaagtcgat gaagagggaa agttcgtgcg gctgcgcaac aagtccaacg aggaccagtc
     5221 catgggcaac tggcagatca ggcgtcagaa tggtgacgat cctttgatga cctatcgctt
     5281 cccaccgaag ttcaccctaa aggctgggca ggtggtgacg atctgggctt caggagctgg
     5341 ggccacccat agccccccta ctgacttggt gtggaaggcg cagaacacct ggggctgtgg
     5401 gagcagcctt cgcaccgctc tcatcaactc cactggagaa gaagtggcca tgcgcaagct
     5461 ggtgcgctca ctgaccatgg ttgaggacaa tgaggatgac gacgaggatg gagaagagct
     5521 cctccatcac caccgtggtt cccactgcag cggctcgggg gaccccgctg agtacaacct
     5581 gcgctcacgc accgtgctgt gcgggacgtg tgggcagcct gctgacaagg ctgccggtgg
     5641 agcgggagcc caggtgggcg gatccatctc ctctggctct tctgcctcca gtgtcacagt
     5701 cactcgaagc ttccgcagtg tggggggcag tgggggtggc agcttcgggg acaacctagt
     5761 cacccgctcc tacctcctgg gcaactccag tccccggagc cagagctccc agaactgcag
     5821 catcatgtaa aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttcgatcgaa
     5881 ccgcggtcta gactcgagac ctggaaaaac atggagcaat cacaagtagc aacacagcag
     5941 ctaccaatgc tgcttgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     6001 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6061 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     6121 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     6181 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     6241 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     6301 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     6361 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     6421 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6481 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6541 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     6601 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6661 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6721 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6781 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6841 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     6901 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6961 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7021 cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc
     7081 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7141 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7201 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt
     7261 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     7321 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg
     7381 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7501 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7561 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     7621 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     7681 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     7741 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     7801 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     7861 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     7921 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     7981 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     8041 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     8101 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     8161 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8221 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     8281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8401 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     8461 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8521 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8581 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8641 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8701 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8761 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8821 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8881 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8941 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9001 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     9061 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9601 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9661 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9721 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9781 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9841 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9901 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9961 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10021 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10081 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10141 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10201 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10261 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10321 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10381 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10441 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10501 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10561 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
