LOCUS       LeGO-BSD-5LO_Cop        9421 bp    DNA     circular UNA 08-FEB-2022
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...10-efu6w4d
VERSION     urn.local...10-efu6w4d
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    10..611
                     /locus_tag="IRES"
                     /note="Geneious type: polylinker"
                     /standard_name="IRES"
     gene            <612..1007
                     /note="BSD"
                     /standard_name="BSD gene"
     protein_bind    1059..1092
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /standard_name="loxP"
     misc_binding    1148..1736
                     /note="WPRE"
     misc_feature    1944..2445
                     /locus_tag="3'SIN LTR"
                     /standard_name="3' LTR"
     misc_feature    complement(2448..3121)
                     /locus_tag="pUC"
                     /standard_name="ori"
     CDS             complement(3266..4126)
                     /locus_tag="AmpR"
                     /standard_name="AmpR"
     promoter        complement(4127..4231)
                     /gene="bla"
                     /note="AmpR promoter; color: #ffffff; direction: LEFT"
                     /standard_name="AmpR promoter"
     enhancer        4497..4876
                     /note="human cytomegalovirus immediate early enhancer"
                     /standard_name="CMV enhancer"
     promoter        4878..5076
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /standard_name="CMV promoter"
     LTR             5087..5275
                     /note="5' LTR (R and U5 portions; U3 was replaced by the
                     CMV promoter)"
                     /standard_name="LTR"
     misc_feature    5321..5446
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /standard_name="HIV-1 Psi"
     misc_feature    5947..6187
                     /locus_tag="RRE"
                     /standard_name="RRE"
     misc_feature    6681..>6855
                     /locus_tag="cPPT"
                     /standard_name="cPPT"
     protein_bind    6939..6972
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /standard_name="loxP"
     promoter        7043..7449
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /standard_name="SFFV promoter"
     regulatory      7454..7465
                     /standard_name="Kozac-Sequenz; Kozak-Sequenz"
     CDS             7463..9415
                     /standard_name="5-LO"
ORIGIN      
        1 gctacgtaaa ttccgccccc cccccccctc tccctccccc ccccctaacg ttactggccg
       61 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
      121 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
      181 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
      241 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
      301 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
      361 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
      421 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
      481 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
      541 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
      601 tggccacaac cgccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
      661 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
      721 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
      781 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
      841 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
      901 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
      961 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaaagt aaagcggccg
     1021 gccgccagca cagtggtcga aattcgtcga gggacctaat aacttcgtat agcatacatt
     1081 atacgaagtt atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct
     1141 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1201 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1261 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1321 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1381 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1441 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1501 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1561 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1621 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1681 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     1741 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     1801 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     1861 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     1921 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     1981 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     2041 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     2101 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     2161 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     2221 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     2281 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2341 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2401 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag
     2461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2941 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3601 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3661 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3721 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3781 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3841 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3901 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3961 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4021 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4081 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4141 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4201 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4261 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     4321 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     4381 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     4441 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     4501 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     4561 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     4621 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     4681 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     4741 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     4801 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     4861 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     4921 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     4981 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     5041 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     5101 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5161 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5221 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     5281 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     5341 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     5401 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     5461 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     5521 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     5581 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     5641 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     5701 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     5761 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     5821 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     5881 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     5941 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     6001 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     6061 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     6121 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     6181 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     6241 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     6301 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     6361 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     6421 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     6481 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     6541 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     6601 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     6661 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     6721 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     6781 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     6841 agcagagatc cagtttggtt agtaccgggc ccgctctagt cgaggtcgac ggtatcgata
     6901 agctcgcttc acgagattcc agcaggtcga gggacctaat aacttcgtat agcatacatt
     6961 atacgaagtt atattaaggg ttccaagctt aagcggccgg ccgctgaaag accccacctg
     7021 taggtttggc aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc
     7081 aagaatagag aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac
     7141 aggatatctg cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc
     7201 agtttcggcc ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag
     7261 cagtttctta agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc
     7321 gccttatttg aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc
     7381 cgagctctat aaaagagctc acaacccctc actcggcgcg ccagtcctcc gattgactga
     7441 gtcgcccgga tccgccgcca ccatgcccag ctacaccgtg accgtggcca ccggctctca
     7501 gtggttcgcc ggcaccgacg actacatcta cctgagcctg gtcggaagcg ccggctgcag
     7561 cgagaaacat ctgctggaca agcccttcta caacgacttc gagcggggag ccgtggactc
     7621 ctacgatgtg accgtggacg aggaactggg cgagatccag ctggtccgca tcgagaagcg
     7681 gaagtactgg ctgaacgacg actggtatct gaagtacatc accctgaaaa ccccccacgg
     7741 cgactacatc gagttccctt gctaccggtg gattacagga gacgtggaag tggtgctgcg
     7801 ggacggcaga gccaagctgg ccagggacga ccagatccac atcctgaagc agcaccggcg
     7861 gaaagagctg gaaacccggc agaaacagta ccgctggatg gaatggaacc ccggcttccc
     7921 cctgtccatc gacgccaagt gccacaagga cctgccccgg gacatccagt tcgacagcga
     7981 gaagggcgtg gacttcgtgc tgaactacag caaggccatg gaaaacctgt tcatcaaccg
     8041 gttcatgcac atgttccaga gcagctggaa cgacttcgcc gacttcgaga agatcttcgt
     8101 gaagatcagc aacaccatca gcgagagagt gatgaaccac tggcaggaag atctgatgtt
     8161 cggctaccag ttcctgaacg gctgcaaccc cgtgctgatc cggcggtgta ccgagctgcc
     8221 tgagaagctg cccgtgacca ccgagatggt ggaatgcagc ctggaacggc agctgagcct
     8281 ggaacaggaa gtgcagcagg gcaacatctt catcgtggat ttcgagctgc tggacggcat
     8341 tgacgccaac aagaccgacc cctgcaccct gcagtttctg gccgctccca tctgcctgct
     8401 gtacaagaac ctggccaaca agatcgtgcc tatcgccatc cagctgaacc agatccccgg
     8461 cgacgagaac cccatcttcc tgcccagcga cgccaaatac gactggctgc tggccaagat
     8521 ctgggtccgc tccagcgact tccacgtgca ccagaccatc acccatctgc tgcggaccca
     8581 cctggtgtcc gaggtgttcg gaatcgccat gtacagacag ctgcccgccg tgcaccctat
     8641 cttcaagctg ctggtggccc acgtgcggtt cacaatcgcc atcaacacca aggccagaga
     8701 gcagctgatc tgcgagtgcg gcctgttcga caaggccaac gccacaggcg gcggaggcca
     8761 cgtgcagatg gtgcagagag ccatgaagga cctgacctac gccagcctgt gcttccccga
     8821 ggccatcaag gccaggggca tggaaagcaa agaggacatc ccctactact tctaccggga
     8881 cgacggcctg ctggtctggg aggccatcag aaccttcacc gccgaggtgg tggacatcta
     8941 ctacgagggc gaccaggtgg tggaagagga ccccgagctg caggacttcg tgaacgacgt
     9001 gtacgtgtac ggcatgcggg gcagaaagag cagcggcttc cccaagagcg tgaacttcgg
     9061 ccagtacgac tggtgcagct ggatccccaa cgcccctccc accatgagag cccccccacc
     9121 tacagccaag ggcgtggtga caatcgagca gatcgtggac accctgcccg accggggcag
     9181 aagctgttgg catctgggag ccgtgtgggc cctgagccag ttccaggaaa acgagctgtt
     9241 cctgggcatg taccccgagg aacactttat cgagaagccc gtgaaagaag ccatggcccg
     9301 gttccggaag aacctggaag ccatcgtgtc cgtgatcgcc gagcggaaca agaagaagca
     9361 gctgccctac tactacctgt cccccgacag aatccctaac agcgtggcca tctgagcggc
     9421 c
//
