LOCUS       S074_map                4444 bp ds-DNA     circular     28-JAN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        20..39
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    97..99
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             100..1740
                     /label="Translation 100-1740"
     misc_feature    1741..1782
                     /label="Myc Tag"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1741..1795
                     /label="Translation 1741-1795"
     misc_feature    1783..1788
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1790..1795
                     /label="Geneious type: restriction site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1846..1863)
                     /label="T7 Rev"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2003..2458
                     /label="f1 origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacatA TGCATCATCA TCATCATCAT
      121 GGTTATCCCT ATGATGTTCC AGATTATGCT GGGGCCCAGC CGGCCAGATC TGCGAACGAC
      181 ACCGTAGTTG TGGGCTCGAT CGAGTTTACA GAAGGGATTA TCGTCGCAAA CATGGTGGCA
      241 GAGATGATTG AGGCGCATAC AGACCTTAAG GTGGTTCGCA AACTGAACCT TGGCGGGCCG
      301 AACGTTAACT TTGAAGCCAT TAAACGCGGA GGTGCGAATA ATGGTATTGA CATTTACGTG
      361 GAGTACACTG GGTCCGGTCT TGTGGATATT CTGGGGTTCC CGGAGCCGAA CGTCTATATC
      421 ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCGAACTTCA AGATCCGCCA CAACATGGAG
      481 GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
      541 GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG TGCTGAGCAA AGACCCCAAC
      601 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
      661 ATGGACGAGC TGTACAAGGG CGGTACCGGA GGGAGCATGA GCAAGGGCGA GGAGCTGTTC
      721 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGGCG TAAACGGCCA CAAGTTCAGC
      781 GTGCGCGGCG AGGGCGAGGG CGATGCCACC AACGGCAAGC TGACCCTGAA GTTCATCTGC
      841 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG
      901 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG
      961 CCCGAAGGCT ACGTCCAGGA GCGCACCATC AGCTTCAAGG ACGACGGCAC CTACAAGACC
     1021 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC
     1081 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTT TCCGCCGCCC
     1141 AACTCTACTG ATCCAGAAGG TGCATACGAA ACCGTGAAGA AGGAGTACAA ACGTAAATGG
     1201 AATATTGTAT GGCTCAAACC ACTGGGATTC AACAATACGT ATACGCTTGG GGTTAAAGAC
     1261 GAACTGGCGA AACAGTATAA CCTTAAAACC TTCAGTGACT TAGCGAAAAT CTCGGATAAG
     1321 CTGATTCTGG GTGCAACGAT GTTCTTTTTA GAAGGGCCCG ATGGTTACCC AGGCCTGCAA
     1381 AAACTGTACA ATTTCAAATT CAAGCACACC AAAAGCATGG ACATGGGTAT TCGCTATACC
     1441 GCCATTGATA ATAACGAAGT TCAGGTAATT GATGCCTGGG CCACTGATGG CTTGCTGGTG
     1501 AGCCACAAAT TAAAAATTCT GGAGGATGAT AAAGCGTTCT TCCCGCCGTA TTATGCTGCC
     1561 CCCATCATCC GTCAGGATGT CTTAGATAAG CATCCTGAAC TGAAGGACGT GCTGAACAAA
     1621 CTCGCGAATC AAATTTCACT GGAAGAAATG CAGAAACTGA ATTACAAGGT GGACGGTGAG
     1681 GGTCAGGACC CAGCGAAAGT AGCTAAGGAG TTTTTGAAAG AGAAAGGTTT AATTCTGCAG
     1741 gtcgacgaac aaaaactcat ctcagaagag gatctgaatt aagaattcga agcttgatcc
     1801 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     1861 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     1921 tatatccgga tctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1981 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2041 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     2101 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     2161 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2221 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2281 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2341 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     2401 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     2461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4321 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4381 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4441 gcag
//