LOCUS       pCMV(MinDis)-iEscSnFR_P 5200 bp ds-DNA     circular     07-FEB-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..5200
                     /label="source"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     enhancer        9..388
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        389..592
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     543..563
                     /label="CMV-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        637..655
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     637..656
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     sig_peptide     736..798
                     /label="Ig-kappa leader"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             736..2373
                     /label="Translation 736-2373"
     CDS             2374..2403
                     /label="Myc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     exon            2374..2403
                     /label="Translation 2374-2403"
     CDS             2404..2609
                     /label="Translation 2404-2609"
     CDS             2608..2610
                     /label="Translation 2608-2610"
     primer_bind     complement(2629..2646)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     polyA_signal    2635..2859
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(2991..3579)
                     /label="ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(3071..3090)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(3750..4610)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(3750..4610)
                     /label="Translation 4610-3750"
     primer_bind     4373..4392
                     /label="Amp-R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        complement(4611..4715)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      4742..5197
                     /label="f1 ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4829..4848)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     5039..5060
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      721 ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt
      781 ccaggttcca ctggtgacag atctGCGAAC GACACCGTAG TTGTGGGCTC GATCGAGTTT
      841 ACAGAAGGGA TTATCGTCGC AAACATGGTG GCAGAGATGA TTGAGGCGCA TACAGACCTT
      901 AAGGTGGTTC GCAAACTGAA CCTTGGCGGG GAGAACGTTA ACTTTGAAGC CATTAAACGC
      961 GGAGGTGCGA ATAATGGTAT TGACATTTAC GTGGAGTACA CTGGGCACGG TCTTGTGGAT
     1021 ATTCTGGGGT TCCCGGAGCC GAACGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC
     1081 AAGGCGAACT TCAAGATCCG CCACAACATG GAGGACGGCA GCGTGCAGCT CGCCGACCAC
     1141 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
     1201 AGCACCCAGT CCGTGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
     1261 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GGGCGGTACC
     1321 GGAGGGAGCA TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG
     1381 CTGGACGGCG GCGTAAACGG CCACAAGTTC AGCGTGCGCG GCGAGGGCGA GGGCGATGCC
     1441 ACCAACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
     1501 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC
     1561 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
     1621 ATCAGCTTCA AGGACGACGG CACCTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
     1681 ACCCTGGtGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
     1741 GGGCACAAGC TGGAGTACAA CTTTCCGCCG CCCAGCTCTA CTGATCCAGA AGGTGCATAC
     1801 GAAACCGTGA AGAAGGAGTA CAAACGTAAA TGGAATATTG TATGGCTCAA ACCACTGGGA
     1861 TTCAACAATA CGTATACGCT TACCGTTAAA GACGAACTGG CGAAACAGTA TAACCTTAAA
     1921 ACCTTCAGTG ACTTAGCGAA AATCTCGGAT AAGCTGATTC TGGGTGCAAC GATGTTCTTT
     1981 TTAGAAGGGC CCGATGGTTA CCCAGGCCTG CAAAAACTGT ACAATTTCAA ATTCAAGCAC
     2041 ACCAAAAGCA TGGACATGGG TATTCGCTAT ACCGCCATTG ATAATAACGA AGTTCAGGTA
     2101 ATTGATGCCT GGGCCACTGA TGGCTTGCTG GTGAGCCACA AATTAAAAAT TCTGGAGGAT
     2161 GATAAAGAGT TCTTCCCGCC GTATTATGCT GCCCCCATCA TCCGTCAGGA TGTCTTAGAT
     2221 AAGCATCCTG AACTGAAGGA CGTGCTGAAC AAACTCGCGA ATCAAATTTC ACTGGAAGAA
     2281 ATGCAGAAAC TGAATTACAA GGTGGACGGT GAGGGTCAGG ACCCAGCGAA AGTAGCTAAG
     2341 GAGTTTTTGA AAGAGAAAGG Tttaattacg cgtgaacaaa aactcatctc agaagaggat
     2401 ctgaatgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt
     2461 aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc
     2521 atcctcatca aatacttgca gaagaggcgg gaaaggcgac gccaggagaa cgctaactcc
     2581 ttttgctatg aaaatgaggt agccctgtag gcggccgctc gagatcagcc tcgactgtgc
     2641 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2701 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2761 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2821 acaatagcag gcgtgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2881 gtggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2941 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3001 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3061 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3121 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3181 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3241 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3301 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3361 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3421 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3481 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3541 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3601 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3661 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3721 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3781 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3841 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3901 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3961 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4021 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4081 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4141 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4201 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4261 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4321 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4381 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4441 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgtacccaac
     4501 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4561 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4621 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4681 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4741 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4801 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4861 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4921 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4981 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5041 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5101 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5161 aacgcgaatt ttaacaaaat attaacgctt acaatttacg
//