LOCUS       pCMV(MinDis)-iEscSnFR_c 6610 bp ds-DNA     circular     07-FEB-2022
DEFINITION  .
ACCESSION   urn:local:.:1506620526970.23                                       
FEATURES             Location/Qualifiers
     misc_feature    2..597
                     /label="hCMV promoter/enhancer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    545..549
                     /label="CAAAT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    578..584
                     /label="TATA box"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     637..656
                     /label="T7 promoter/priming site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     sig_peptide     736..738
                     /label="sig_peptide"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             736..738
                     /label="Translation 736-738"
     CDS             739..2286
                     /label="Translation 739-2286"
     CDS             2287..2295
                     /label="Translation 2287-2295"
     misc_feature    2296..2337
                     /label="Strong NES"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2296..2337
                     /label="Translation 2296-2337"
     CDS             2338..2340
                     /label="Translation 2338-2340"
     misc_feature    2338..2356
                     /label="motif"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2359..2376)
                     /label="r.0.401"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2424..2440)
                     /label="motif"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      complement(2703..3385)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5163..5822)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            complement(5163..5822)
                     /label="Translation 5822-5163"
     rep_origin      complement(6152..6608)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      721 ttggggatat ccaccatgAC CGTAGTTGTG GGCTCGATCG AGTTTACAGA AGGGATTATC
      781 GTCGCAAACA TGGTGGCAGA GATGATTGAG GCGCATACAG ACCTTAAGGT GGTTCGCAAA
      841 CTGAACCTTG GCGGGGAGAA CGTTAACTTT GAAGCCATTA AACGCGGAGG TGCGAATAAT
      901 GGTATTGACA TTTACGTGGA GTACACTGGG CACGGTCTTG TGGATATTCT GGGGTTCCCG
      961 GAGCCGAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC GAACTTCAAG
     1021 ATCCGCCACA ACATGGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC
     1081 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGTG
     1141 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC
     1201 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGCG GTACCGGAGG GAGCATGAGC
     1261 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGGCGTA
     1321 AACGGCCACA AGTTCAGCGT GCGCGGCGAG GGCGAGGGCG ATGCCACCAA CGGCAAGCTG
     1381 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
     1441 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
     1501 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCAG CTTCAAGGAC
     1561 GACGGCACCT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGtGAACCGC
     1621 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
     1681 TACAACTTTC CGCCGCCCAG CTCTACTGAT CCAGAAGGTG CATACGAAAC CGTGAAGAAG
     1741 GAGTACAAAC GTAAATGGAA TATTGTATGG CTCAAACCAC TGGGATTCAA CAATACGTAT
     1801 ACGCTTACCG TTAAAGACGA ACTGGCGAAA CAGTATAACC TTAAAACCTT CAGTGACTTA
     1861 GCGAAAATCT CGGATAAGCT GATTCTGGGT GCAACGATGT TCTTTTTAGA AGGGCCCGAT
     1921 GGTTACCCAG GCCTGCAAAA ACTGTACAAT TTCAAATTCA AGCACACCAA AAGCATGGAC
     1981 ATGGGTATTC GCTATACCGC CATTGATAAT AACGAAGTTC AGGTAATTGA TGCCTGGGCC
     2041 ACTGATGGCT TGCTGGTGAG CCACAAATTA AAAATTCTGG AGGATGATAA AGAGTTCTTC
     2101 CCGCCGTATT ATGCTGCCCC CATCATCCGT CAGGATGTCT TAGATAAGCA TCCTGAACTG
     2161 AAGGACGTGC TGAACAAACT CGCGAATCAA ATTTCACTGG AAGAAATGCA GAAACTGAAT
     2221 TACAAGGTGG ACGGTGAGGG TCAGGACCCA GCGAAAGTAG CTAAGGAGTT TTTGAAAGAG
     2281 AAAGGTttaa ttctggatat tgatgaactg gcgctgaaat ttgcgggcct ggatctgtga
     2341 gcggccgctc gagatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2401 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2461 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     2521 ggcaggacag caagggggag gattgggaag acaatagcag gcgtgctggg gatgcggtgg
     2581 gctctatggc ttctgaggcg gaaagaacca gtggcggtaa tacggttatc cacagaatca
     2641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2881 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3121 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca
     3481 gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg
     3541 ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg
     3601 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc
     3661 gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc
     3721 cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc
     3781 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
     3841 gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg
     3901 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3961 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     4021 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     4081 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     4141 catcgccatg ggtcacgacg agatcctcgc cgtcgggctt gctcgccttg agcctggcga
     4201 acagttcggc tggcgcgagc ccctgatgct cttgatcatc ctgatcgaca agaccggctt
     4261 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     4321 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     4381 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     4441 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     4501 acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga
     4561 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     4621 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     4681 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcgatct
     4741 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     4801 gaggaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     4861 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     4921 gctgactaat tgagatgctt gctttgcata cttctgcctg ctggggagcc tggggacttt
     4981 ccacacctgg ttgctgacta attgagatgc ttgctttgca tacttctgcc tgctggggag
     5041 cctggggact ttccacaccc taactgacac acattccaca gctggttctt tccgcctcag
     5101 gactcttcct ttttcaataa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5161 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5221 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5281 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5341 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5401 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5461 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5521 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5581 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5641 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5701 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5761 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5821 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5881 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5941 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6001 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6061 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6121 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     6181 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6241 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6301 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6361 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6421 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6481 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6541 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6601 acaatttacg
//