LOCUS       Exported                6145 bp DNA     circular SYN 07-FEB-2022
DEFINITION  pcDNA3.1(+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        236..852
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
     enhancer        315..602
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
     primer_bind     769..789
                     /locus_tag="LGC_CMV-F"
                     /label=LGC_CMV-F
     primer_bind     785..809
                     /locus_tag="LGC_pCEP4for"
                     /label=LGC_pCEP4for
     primer_bind     798..817
                     /locus_tag="LGC_EGFP-N1-F"
                     /label=LGC_EGFP-N1-F
     primer_bind     863..882
                     /locus_tag="LGC_T7prom"
                     /label=LGC_T7prom
     promoter        863..881
                     /locus_tag="T7_promoter"
                     /label=T7_promoter
     misc_feature    883..916
                     /locus_tag="MSC"
                     /label=MSC
     misc_feature    917..922
                     /locus_tag="KpnI"
                     /label=KpnI
     misc_feature    923..1645
                     /locus_tag="mApple"
                     /label=mApple
     primer_bind     970..991
                     /locus_tag="mCherry2 (begin) fw"
                     /label=mCherry2 (begin) fw
     primer_bind     1547..1570
                     /locus_tag="mCherry2 (end) fw"
                     /label=mCherry2 (end) fw
     misc_feature    1646..1651
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    1652..1701
                     /locus_tag="MSC(1)"
                     /label=MSC
                     /label=MSC(1)
     misc_feature    1702..1707
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    1708..1716
                     /locus_tag="MSC(2)"
                     /label=MSC
                     /label=MSC(2)
     primer_bind     1717..1736
                     /locus_tag="P50 - BiDI seq (+) rev"
                     /label=P50 - BiDI seq (+) rev
     primer_bind     complement(1737..1756)
                     /locus_tag="LGC_pcDNA3.1-R"
                     /label=LGC_pcDNA3.1-R
     primer_bind     1739..1756
                     /locus_tag="BGH-R"
                     /label=BGH-R
     terminator      1742..1969
                     /locus_tag="bGH_PA_terminator"
                     /label=bGH_PA_terminator
     polyA_signal    1745..1969
                     /locus_tag="bGH poly(A)signal"
                     /label=bGH poly(A)signal
     primer_bind     complement(1774..1793)
                     /locus_tag="LGC_UniV5his-R"
                     /label=LGC_UniV5his-R
     primer_bind     complement(1868..1887)
                     /locus_tag="LGC_F1-oriR"
                     /label=LGC_F1-oriR
     rep_origin      2032..2338
                     /locus_tag="f1_origin"
                     /label=f1_origin
     promoter        2448..2791
                     /locus_tag="SV40 promotor"
                     /label=SV40 promotor
     primer_bind     complement(2452..2472)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
     enhancer        2458..2673
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     promoter        2470..2738
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
     rep_origin      2637..2714
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
     primer_bind     2699..2718
                     /locus_tag="SV40pro_F"
                     /label=SV40pro_F
     primer_bind     2742..2759
                     /locus_tag="LGC_SV40-Do"
                     /label=LGC_SV40-Do
     CDS             2856..3647
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     terminator      3824..3943
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(3826..3848)
                     /locus_tag="LGC_EGFP-C1-R"
                     /label=LGC_EGFP-C1-R
     primer_bind     complement(3897..3917)
                     /locus_tag="SV40 R"
                     /label=SV40 R
     primer_bind     3897..3917
                     /locus_tag="LGC_SV40"
                     /label=LGC_SV40
     primer_bind     complement(3912..3931)
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(3979..4002)
                     /locus_tag="LGC-M13-24R-BLUE"
                     /label=LGC-M13-24R-BLUE
     primer_bind     complement(3987..4007)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3987..4005)
                     /locus_tag="LGC_Bluescript R"
                     /label=LGC_Bluescript R
     primer_bind     complement(3990..4007)
                     /locus_tag="LGC_M13-29R"
                     /label=LGC_M13-29R
     misc_feature    complement(4004..4026)
                     /locus_tag="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(4004..4024)
                     /locus_tag="LGC_M13-24R"
                     /label=LGC_M13-24R
     primer_bind     complement(4005..4024)
                     /locus_tag="LGC_pQE-F"
                     /label=LGC_pQE-F
     misc_binding    complement(4013..4035)
                     /locus_tag="LacO"
                     /label=LacO
     protein_bind    4015..4031
                     /locus_tag="lac operator"
                     /label=lac operator
     promoter        complement(4040..4069)
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(4052..4075)
                     /locus_tag="LGC_MKSF"
                     /label=LGC_MKSF
     primer_bind     complement(4080..4099)
                     /locus_tag="LGC_M13rev2"
                     /label=LGC_M13rev2
     protein_bind    4084..4105
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
     primer_bind     complement(4360..4379)
                     /locus_tag="LGC_ASK-FN2"
                     /label=LGC_ASK-FN2
     CDS             complement(5149..6009)
                     /codon_start=1
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6051..6079)
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     primer_bind     6098..6117
                     /locus_tag="LGC_sCOS1F"
                     /label=LGC_sCOS1F
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatggtgag caagggcgag gagaataaca tggccatcat
      961 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     1021 gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt
     1081 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     1141 ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
     1201 cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa
     1261 ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa
     1321 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga
     1381 gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa
     1441 ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     1501 gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta
     1561 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     1621 gctgtacaag ggtggcggag ggtccggatc cactagtcca gtgtggtgga attctgcaga
     1681 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     1741 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1801 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1861 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     1921 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     1981 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     2041 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2101 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2161 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2221 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2281 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2341 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2401 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     2461 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2701 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     2761 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     2821 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     2881 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     2941 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3001 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3061 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3121 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3181 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3241 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     3301 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3361 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     3421 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3481 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3541 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3601 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3661 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3721 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3781 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     3841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     3961 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4021 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4081 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4921 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6121 tccccgaaaa gtgccacctg acgtc
//
