LOCUS       Exported                4763 bp DNA     circular SYN 07-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..589
                     /locus_tag="CMV promotor"
                     /label=CMV promotor
     misc_feature    519..539
                     /locus_tag="CMV-F"
                     /label=CMV-F
     misc_feature    658..711
                     /locus_tag="MyrPalm"
                     /label=MyrPalm
     misc_feature    712..721
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    718..1425
                     /locus_tag="mApple"
                     /label=mApple
     rep_origin      complement(1811..2117)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     promoter        complement(2196..2224)
                     /locus_tag="AmpR promotor"
                     /label=AmpR promotor
     enhancer        2296..2511
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     promoter        2428..2624
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     rep_origin      2475..2552
                     /locus_tag="SV40 origin"
                     /label=SV40 origin
     CDS             2662..3453
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     terminator      3628..3897
                     /locus_tag="TK PA terminator"
                     /label=TK PA terminator
     polyA_signal    3685..3732
                     /locus_tag="HSV TK poly(A)signal"
                     /label=HSV TK poly(A)signal
     rep_origin      3985..4667
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcggaccatg
      661 ggatgtatta aatcaaaaag gaaagacaat ctcaatgacg atgaaccggt cgccaccatg
      721 gtgagcaagg gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      781 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      841 ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc
      901 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggtctacat taagcaccca
      961 gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg
     1021 atgaacttcg aggacggcgg cattattcac gttaaccagg actcctccct gcaggacggc
     1081 gtgttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1141 cagaagaaga ccatgggctg ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc
     1201 ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg gcggccacta cgccgccgag
     1261 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac
     1321 atcaagttgg acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1381 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac
     1441 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     1501 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     1561 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1621 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
     1681 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     1741 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     1801 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     1861 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     1921 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     1981 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2041 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2101 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     2161 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2221 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     2281 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2401 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2461 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2521 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2581 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     2641 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     2701 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     2761 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     2821 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     2881 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     2941 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     3001 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3061 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3121 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3181 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3241 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3301 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3361 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3421 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     3481 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     3541 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     3601 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     3661 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     3721 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     3781 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     3841 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     3901 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3961 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4201 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4741 ataaccgtat taccgccatg cat
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