LOCUS       Exported                8352 bp DNA     circular SYN 20-MAY-2020
DEFINITION  pcDNA3.1(+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        236..852
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
     enhancer        315..602
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
     primer_bind     769..789
                     /locus_tag="LGC_CMV-F"
                     /label=LGC_CMV-F
     primer_bind     785..809
                     /locus_tag="LGC_pCEP4for"
                     /label=LGC_pCEP4for
     primer_bind     798..817
                     /locus_tag="LGC_EGFP-N1-F"
                     /label=LGC_EGFP-N1-F
     primer_bind     863..882
                     /locus_tag="LGC_T7prom"
                     /label=LGC_T7prom
     promoter        863..881
                     /locus_tag="T7_promoter"
                     /label=T7_promoter
     misc_feature    883..894
                     /locus_tag="MSC(2)"
                     /label=MSC
                     /label=MSC(2)
     misc_feature    895..900
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    915..924
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    921..1637
                     /locus_tag="mEYFP woSTOP"
                     /label=mEYFP woSTOP
     primer_bind     complement(984..1002)
                     /locus_tag="LGC_EGFP-N1-R"
                     /label=LGC_EGFP-N1-R
     primer_bind     1562..1580
                     /locus_tag="LGC_EGFP-C1-F"
                     /label=LGC_EGFP-C1-F
     misc_feature    1638..1660
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    1655..1660
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    1661..1666
                     /locus_tag="MSC(1)"
                     /label=MSC
                     /label=MSC(1)
     misc_feature    1661..1666
                     /locus_tag="AflII"
                     /label=AflII
     misc_feature    1668..2375
                     /locus_tag="mCherry2"
                     /label=mCherry2
     misc_feature    2376..2398
                     /locus_tag="Linker(2)"
                     /label=Linker
                     /label=Linker(2)
     misc_feature    2399..2404
                     /locus_tag="KpnI"
                     /label=KpnI
     misc_feature    2406..3122
                     /locus_tag="mEGFP"
                     /label=mEGFP
     primer_bind     complement(2469..2487)
                     /locus_tag="LGC_EGFP-N1-R(1)"
                     /label=LGC_EGFP-N1-R
                     /label=LGC_EGFP-N1-R(1)
     primer_bind     3047..3065
                     /locus_tag="LGC_EGFP-C1-F(1)"
                     /label=LGC_EGFP-C1-F
                     /label=LGC_EGFP-C1-F(1)
     misc_feature    3123..3145
                     /locus_tag="Linker(1)"
                     /label=Linker
                     /label=Linker(1)
     misc_feature    3140..3145
                     /locus_tag="AgeI(1)"
                     /label=AgeI
                     /label=AgeI(1)
     misc_feature    3146..3151
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    3152
                     /locus_tag="MSC"
                     /label=MSC
     misc_feature    3153..3875
                     /locus_tag="mApple"
                     /label=mApple
     misc_feature    3876..3881
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    3876..3881
                     /locus_tag="MSC(5)"
                     /label=MSC
                     /label=MSC(5)
     misc_feature    3882..3908
                     /locus_tag="MSC(3)"
                     /label=MSC
                     /label=MSC(3)
     misc_feature    3909..3914
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    3915..3923
                     /locus_tag="MSC(4)"
                     /label=MSC
                     /label=MSC(4)
     primer_bind     3924..3943
                     /locus_tag="P50 - BiDI seq (+) rev"
                     /label=P50 - BiDI seq (+) rev
     primer_bind     complement(3944..3963)
                     /locus_tag="LGC_pcDNA3.1-R"
                     /label=LGC_pcDNA3.1-R
     primer_bind     3946..3963
                     /locus_tag="BGH-R"
                     /label=BGH-R
     terminator      3949..4176
                     /locus_tag="bGH_PA_terminator"
                     /label=bGH_PA_terminator
     polyA_signal    3952..4176
                     /locus_tag="bGH poly(A)signal"
                     /label=bGH poly(A)signal
     primer_bind     complement(3981..4000)
                     /locus_tag="LGC_UniV5his-R"
                     /label=LGC_UniV5his-R
     primer_bind     complement(4075..4094)
                     /locus_tag="LGC_F1-oriR"
                     /label=LGC_F1-oriR
     rep_origin      4239..4545
                     /locus_tag="f1_origin"
                     /label=f1_origin
     promoter        4655..4998
                     /locus_tag="SV40 promotor"
                     /label=SV40 promotor
     primer_bind     complement(4659..4679)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
     enhancer        4665..4880
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     promoter        4677..4945
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
     rep_origin      4844..4921
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
     primer_bind     4906..4925
                     /locus_tag="SV40pro_F"
                     /label=SV40pro_F
     primer_bind     4949..4966
                     /locus_tag="LGC_SV40-Do"
                     /label=LGC_SV40-Do
     CDS             5063..5854
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     terminator      6031..6150
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(6033..6055)
                     /locus_tag="LGC_EGFP-C1-R"
                     /label=LGC_EGFP-C1-R
     primer_bind     complement(6104..6124)
                     /locus_tag="SV40 R"
                     /label=SV40 R
     primer_bind     6104..6124
                     /locus_tag="LGC_SV40"
                     /label=LGC_SV40
     primer_bind     complement(6119..6138)
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(6186..6209)
                     /locus_tag="LGC-M13-24R-BLUE"
                     /label=LGC-M13-24R-BLUE
     primer_bind     complement(6194..6214)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(6194..6212)
                     /locus_tag="LGC_Bluescript R"
                     /label=LGC_Bluescript R
     primer_bind     complement(6197..6214)
                     /locus_tag="LGC_M13-29R"
                     /label=LGC_M13-29R
     misc_feature    complement(6211..6233)
                     /locus_tag="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(6211..6231)
                     /locus_tag="LGC_M13-24R"
                     /label=LGC_M13-24R
     primer_bind     complement(6212..6231)
                     /locus_tag="LGC_pQE-F"
                     /label=LGC_pQE-F
     misc_binding    complement(6220..6242)
                     /locus_tag="LacO"
                     /label=LacO
     protein_bind    6222..6238
                     /locus_tag="lac operator"
                     /label=lac operator
     promoter        complement(6247..6276)
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
                     /note="/translation=MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTV
                     KHEEA 
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA 
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ 
                     PPHCISHDGYFLGRSKVR*"
     primer_bind     complement(6259..6282)
                     /locus_tag="LGC_MKSF"
                     /label=LGC_MKSF
     primer_bind     complement(6287..6306)
                     /locus_tag="LGC_M13rev2"
                     /label=LGC_M13rev2
     protein_bind    6291..6312
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
     primer_bind     complement(6567..6586)
                     /locus_tag="LGC_ASK-FN2"
                     /label=LGC_ASK-FN2
     CDS             complement(7356..8216)
                     /codon_start=1
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8258..8286)
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     primer_bind     8305..8324
                     /locus_tag="LGC_sCOS1F"
                     /label=LGC_sCOS1F
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 acgatgaacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      961 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1021 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1081 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc
     1141 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1201 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1261 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1321 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1381 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1441 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1501 tgctgctgcc cgacaaccac tacctgagct accagtccaa actgagcaaa gaccccaacg
     1561 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1621 tggacgagct gtacaagccg cctgccgctg caccaccggt cttaagcatg gtgagcaagg
     1681 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1741 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1801 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1861 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     1921 ccgactactt gaagctgtcc ttccccgagg gcttcaattg ggagcgcgtg atgaacttcg
     1981 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     2041 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagtgtcgta
     2101 ccatgggctg ggaggcctcc actgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     2161 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     2221 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcgac atcaagttgg
     2281 acatcctttc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     2341 gccactccac cggcggcatg gacgagctgt acaagccgcc tgccgctgca ccaccggtgg
     2401 tacccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2461 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2521 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2581 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2641 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2701 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2761 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2821 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2881 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2941 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3001 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     3061 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3121 agccgcctgc cgctgcacca ccggtggatc ccatggtgag caagggcgag gagaataaca
     3181 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     3241 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
     3301 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     3361 tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc
     3421 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
     3481 ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc
     3541 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     3601 cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc
     3661 tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
     3721 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
     3781 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     3841 gcatggacga gctgtacaaa ggtggcggag ggtccgaatt ctgcagatat ccagcacagt
     3901 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     3961 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4021 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4081 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4141 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     4201 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4261 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4321 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4381 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4441 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4501 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4561 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4621 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4681 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4741 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4801 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4861 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4921 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4981 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     5041 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     5101 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5161 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5221 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5281 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5341 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5401 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5461 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5521 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5581 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5641 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5701 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5761 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5821 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5881 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5941 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     6001 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     6061 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6121 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     6181 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6301 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7021 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
     7141 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7201 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7261 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7321 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7381 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7441 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7501 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7561 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7621 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7681 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7741 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7801 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7861 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7921 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7981 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8041 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8101 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8161 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8221 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8281 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8341 ccacctgacg tc
//
