LOCUS       Exported                5120 bp DNA     circular SYN 07-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..589
                     /locus_tag="CMV promotor"
                     /label=CMV promotor
     promoter        10..562
                     /locus_tag="CMV immearly promotor"
                     /label=CMV immearly promotor
     enhancer        65..352
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
     primer_bind     519..539
                     /locus_tag="CMV-F"
                     /label=CMV-F
     protein_bind    633..642
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     sig_peptide     639..713
                     /locus_tag="GPIsp"
                     /label=GPIsp
     misc_feature    750..759
                     /locus_tag="Kozak sequence(1)"
                     /label=Kozak sequence
                     /label=Kozak sequence(1)
     misc_feature    756..1463
                     /locus_tag="mCherry2"
                     /label=mCherry2
     gene            1464..1571
                     /locus_tag="GPI"
                     /label=GPI
     primer_bind     1984..2004
                     /locus_tag="SV40"
                     /label=SV40
     rep_origin      complement(2168..2474)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     promoter        2553..2581
                     /locus_tag="AmpR promotor"
                     /label=AmpR promotor
     enhancer        2653..2868
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     promoter        2785..2981
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     rep_origin      2832..2909
                     /locus_tag="SV40 origin"
                     /label=SV40 origin
     CDS             3019..3810
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     terminator      3985..4254
                     /locus_tag="TK PA terminator"
                     /label=TK PA terminator
     polyA_signal    4042..4089
                     /locus_tag="HSV TK poly(A)signal"
                     /label=HSV TK poly(A)signal
     rep_origin      4342..5024
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttagc tagccaccat ggagctcttt tggagtatag
      661 tctttactgt cctcctgagt ttctcctgcc gggggtcaga ctgggaatct ctgcagtcga
      721 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata
      781 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      841 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      901 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      961 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1021 agctgtcctt ccccgagggc ttcaattggg agcgcgtgat gaacttcgag gacggcggcg
     1081 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1141 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gtgtcgtacc atgggctggg
     1201 aggcctccac tgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1261 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1321 agaagcccgt gcagctgccc ggcgcctaca acgtcgacat caagttggac atcctttccc
     1381 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1441 gcggcatgga cgagctgtac aagtatgatc cgcgcccaag cagcggtcat tctagacaca
     1501 gatatgcact tatacccata ccattagcag taattacaac atgtattgtg ctgtatatga
     1561 atgttcttta attgagaaga caatttcttc atttttaggt attctgaaat gtgacagaaa
     1621 accagacaga accaactcca attgattggt aacagaagat gaagacaaca gcataactaa
     1681 attattttaa aaactaaaaa gccatctgat ttctcatttg agctttagag cacaatggat
     1741 ctcgagggat cttccatacc taccaagttt tgcgccttgc aaggtcgcgg ccgcgactct
     1801 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1861 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1921 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1981 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     2041 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2101 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2161 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2221 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2281 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2341 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2401 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2461 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     2521 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2581 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     2641 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2701 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2761 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2821 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2881 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2941 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     3001 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3061 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3121 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3181 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3241 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3301 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3361 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3421 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3481 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3541 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     3601 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3661 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3721 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3781 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     3841 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3901 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3961 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     4021 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     4081 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     4141 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     4201 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     4261 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4321 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4381 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4441 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4501 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4561 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4621 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4681 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4861 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4981 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5041 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5101 accgtattac cgccatgcat
//
