LOCUS       Exported                7099 bp DNA     circular SYN 07-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   urn.local.KUK.1519638089260.90023
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..255)
                     /locus_tag="bGH PA terminator"
                     /label=bGH PA terminator
     primer_bind     110..129
                     /locus_tag="LGC_F1-oriR"
                     /label=LGC_F1-oriR
     primer_bind     204..223
                     /locus_tag="LGC_UniV5his-R"
                     /label=LGC_UniV5his-R
     primer_bind     241..260
                     /locus_tag="LGC_pcDNA3.1-R"
                     /label=LGC_pcDNA3.1-R
     primer_bind     complement(241..258)
                     /locus_tag="BGH-R(1)"
                     /label=BGH-R
                     /label=BGH-R(1)
     misc_feature    complement(278..280)
                     /locus_tag="STOP"
                     /label=STOP
     misc_feature    complement(281..997)
                     /locus_tag="mEGFP"
                     /label=mEGFP
     misc_feature    complement(338..356)
                     /locus_tag="EGFP-C1-F"
                     /label=EGFP-C1-F
     misc_feature    complement(377..379)
                     /locus_tag="A206K"
                     /label=A206K
     misc_feature    complement(994..1003)
                     /locus_tag="Kozak sequence(1)"
                     /label=Kozak sequence
                     /label=Kozak sequence(1)
     gene            complement(1011..1057)
                     /locus_tag="MyrPalm"
                     /label=MyrPalm
     mRNA            complement(1070..1595)
                     /locus_tag="CCDC142 mRNA"
                     /label=CCDC142 mRNA
     gene            complement(1070..1595)
                     /locus_tag="CCDC142 gene"
                     /label=CCDC142 gene
     CDS             complement(1070..1202)
                     /locus_tag="CCDC142 CDS"
                     /label=CCDC142 CDS
     primer_bind     1070..1088
                     /locus_tag="ccd_ttc promotor ForwP"
                     /label=ccd_ttc promotor ForwP
     primer_bind     complement(1071..1090)
                     /locus_tag="P66 - seq 2D minus R"
                     /label=P66 - seq 2D minus R
     primer_bind     complement(1109..1126)
                     /locus_tag="P49 - BiDi seq (-) rev"
                     /label=P49 - BiDi seq (-) rev
     gene            1397..1703
                     /locus_tag="TTC31 gene"
                     /label=TTC31 gene
     mRNA            1397..1500
                     /locus_tag="TTC31 mRNA(2)"
                     /label=TTC31 mRNA
                     /label=TTC31 mRNA(2)
     mRNA            1438..1500
                     /locus_tag="TTC31 mRNA(3)"
                     /label=TTC31 mRNA
                     /label=TTC31 mRNA(3)
     misc_RNA        1449..1500
                     /locus_tag="TTC31 RNA(1)"
                     /label=TTC31 RNA
                     /label=TTC31 RNA(1)
     misc_RNA        1450..1500
                     /locus_tag="TTC31 RNA"
                     /label=TTC31 RNA
     CDS             1461..1500
                     /locus_tag="TTC31 CDS(1)"
                     /label=TTC31 CDS
                     /label=TTC31 CDS(1)
     primer_bind     1640..1659
                     /locus_tag="P48 - BiDi seq (+) fw"
                     /label=P48 - BiDi seq (+) fw
     primer_bind     1657..1676
                     /locus_tag="P67 - seq 2D plus F"
                     /label=P67 - seq 2D plus F
     mRNA            1687..1703
                     /locus_tag="TTC31 mRNA"
                     /label=TTC31 mRNA
     CDS             1687..1703
                     /locus_tag="TTC31 CDS"
                     /label=TTC31 CDS
     misc_RNA        1687..1703
                     /locus_tag="TTC31 RNA(2)"
                     /label=TTC31 RNA
                     /label=TTC31 RNA(2)
     mRNA            1687..1703
                     /locus_tag="TTC31 mRNA(1)"
                     /label=TTC31 mRNA
                     /label=TTC31 mRNA(1)
     misc_RNA        1687..1703
                     /locus_tag="TTC31 RNA(3)"
                     /label=TTC31 RNA
                     /label=TTC31 RNA(3)
     misc_feature    1716..1762
                     /locus_tag="MyrPalm(1)"
                     /label=MyrPalm
                     /label=MyrPalm(1)
     misc_feature    1770..1779
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    1776..2483
                     /locus_tag="mCherry2"
                     /label=mCherry2
     primer_bind     complement(2502..2521)
                     /locus_tag="P50 - BiDI seq (+) rev"
                     /label=P50 - BiDI seq (+) rev
     primer_bind     complement(2522..2541)
                     /locus_tag="LGC_pcDNA3.1-R(1)"
                     /label=LGC_pcDNA3.1-R
                     /label=LGC_pcDNA3.1-R(1)
     primer_bind     2524..2541
                     /locus_tag="BGH-R"
                     /label=BGH-R
     terminator      2527..2754
                     /locus_tag="bGH_PA_terminator"
                     /label=bGH_PA_terminator
     primer_bind     complement(2559..2578)
                     /locus_tag="LGC_UniV5his-R(1)"
                     /label=LGC_UniV5his-R
                     /label=LGC_UniV5his-R(1)
     primer_bind     complement(2653..2672)
                     /locus_tag="LGC_F1-oriR(1)"
                     /label=LGC_F1-oriR
                     /label=LGC_F1-oriR(1)
     rep_origin      complement(2800..3228)
                     /direction=LEFT
                     /locus_tag="f1 origin"
                     /label=f1 origin
     promoter        3233..3602
                     /locus_tag="SV40 promotor"
                     /label=SV40 promotor
     enhancer        3243..3458
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     rep_origin      3422..3499
                     /locus_tag="SV40 origin"
                     /label=SV40 origin
     primer_bind     3527..3544
                     /locus_tag="LGC_SV40-Do"
                     /label=LGC_SV40-Do
     CDS             3635..4642
                     /locus_tag="hygroB"
                     /label=hygroB
     terminator      4775..4897
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
     primer_bind     complement(4780..4802)
                     /locus_tag="LGC_EGFP-C1-R"
                     /label=LGC_EGFP-C1-R
     primer_bind     4851..4871
                     /locus_tag="LGC_SV40"
                     /label=LGC_SV40
     primer_bind     complement(4933..4956)
                     /locus_tag="LGC-M13-24R-BLUE"
                     /label=LGC-M13-24R-BLUE
     primer_bind     complement(4941..4959)
                     /locus_tag="LGC_Bluescript R"
                     /label=LGC_Bluescript R
     primer_bind     complement(4944..4961)
                     /locus_tag="LGC_M13-29R"
                     /label=LGC_M13-29R
     primer_bind     complement(4958..4978)
                     /locus_tag="LGC_M13-24R"
                     /label=LGC_M13-24R
     primer_bind     complement(4959..4978)
                     /locus_tag="LGC_pQE-F"
                     /label=LGC_pQE-F
     protein_bind    4967..4989
                     /locus_tag="lac operator"
                     /label=lac operator
     promoter        4994..5023
                     /locus_tag="lac promotor"
                     /label=lac promotor
     primer_bind     complement(5006..5029)
                     /locus_tag="LGC_MKSF"
                     /label=LGC_MKSF
     primer_bind     complement(5034..5053)
                     /locus_tag="LGC_M13rev2"
                     /label=LGC_M13rev2
     protein_bind    5038..5059
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
     rep_origin      5288..5958
                     /locus_tag="ori (high copy ColE1, pBR322, pUC)"
                     /label=ori (high copy ColE1, pBR322, pUC)
     primer_bind     complement(5314..5333)
                     /locus_tag="LGC_ASK-FN2"
                     /label=LGC_ASK-FN2
     CDS             complement(6103..6963)
                     /locus_tag="AmpR"
                     /label=AmpR
     promoter        complement(6964..7068)
                     /locus_tag="AmpR promotor"
                     /label=AmpR promotor
     primer_bind     7052..7071
                     /locus_tag="LGC_sCOS1F"
                     /label=LGC_sCOS1F
ORIGIN
        1 gacggatcgg gagatctgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
       61 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
      121 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
      181 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
      241 tagaaggcac agtcgaggct gatcagcaat tgaattctta cttgtacagc tcgtccatgc
      301 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      361 tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      421 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      481 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      541 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      601 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      661 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
      721 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
      781 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      841 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      901 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      961 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt tcatcgtcat
     1021 tgagattgtc tttccttttt gatttaatac atcccatgga tccgattcta gtgaacctcc
     1081 cagcgaagac cgcccgttcg acttctctcc cactgctcct ccccagtgcc cccgggttgc
     1141 gccctcagcg ggggcacgat aacgagtgga ggcaggctac ctgagcgaga cgcctgggcc
     1201 atggggcggc gggtccagaa cgaacctaac gattcccact tccctgcacg gaccaacgcc
     1261 cgccgcagct cggacttcgc cccatcgcaa gagccgtttt ctccagtccg ggagtcgcgg
     1321 ggaccttcat ggactctctc gtgctccgta atgggaggct tctgccccta acaaacatgg
     1381 ccgcccactc tgtcccaccc cgccctttgg ctggtatcaa atactagact acctttcatt
     1441 tccgggtcac tgtagatgcg atggcgccga ttccaaagac tgtggggcgg atcaagctag
     1501 gtgagcggta tgacaagacc agtgggggtc taggagggca gaggcagccc ctcttgggtc
     1561 cacctgtggt ctgaacgttt cgagtaggat tttatgcaga gggcggagag tcgagggtag
     1621 agtggggagg actctagccc atctgtcccg agcccgctga cgaggctccg ccttcctttc
     1681 ctgtagactg ctctctacgg ccctagatcg gtaccatggg atgtattaaa tcaaaaagga
     1741 aagacaatct caatgacgat gaaccggtcg ccaccatggt gagcaagggc gaggaggata
     1801 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1861 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1921 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1981 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2041 agctgtcctt ccccgagggc ttcaattggg agcgcgtgat gaacttcgag gacggcggcg
     2101 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2161 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gtgtcgtacc atgggctggg
     2221 aggcctccac tgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2281 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2341 agaagcccgt gcagctgccc ggcgcctaca acgtcgacat caagttggac atcctttccc
     2401 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2461 gcggcatgga cgagctgtac aaggtataga gctctagagg gcccgtttaa acccgctgat
     2521 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2581 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2641 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2701 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     2761 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     2821 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2881 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2941 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3001 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3061 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3121 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3181 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     3241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3301 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3361 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3481 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     3541 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     3601 cggatctgat cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3661 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3721 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3781 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3841 tccggaagtg cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg
     3901 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3961 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     4021 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     4081 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     4141 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     4201 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     4261 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     4321 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     4381 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     4441 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     4501 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     4561 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4621 cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt
     4681 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4741 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     4801 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4861 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     4921 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4981 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5041 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5761 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5881 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5941 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6001 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6061 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7081 aaaagtgcca cctgacgtc
//
