LOCUS       MyrPalm_mCherry2        7092 bp ds-DNA     circular     09-DEC-2019
DEFINITION  .
ACCESSION   urn.local.KUK.1519638089260.90023                                  
VERSION     urn.local.KUK.1519638089260.90023                                  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_RNA        1438..1488
                     /locus_tag="TTC31 RNA"
                     /label="TTC31 RNA"
                     /ApEinfo_label="TTC31 RNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5281..5951)
                     /locus_tag="pUC origin"
                     /label="pUC origin"
                     /ApEinfo_label="pUC origin"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     2406..2424
                     /locus_tag="LGC_EGFP-C1-F"
                     /label="LGC_EGFP-C1-F"
                     /ApEinfo_label="LGC_EGFP-C1-F"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="#54a9a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4771..4890
                     /locus_tag="SV40_PA_terminator"
                     /label="SV40_PA_terminator"
                     /ApEinfo_label="SV40_PA_terminator"
                     /ApEinfo_fwdcolor="#ae0000"
                     /ApEinfo_revcolor="#804040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1828..1846)
                     /locus_tag="LGC_EGFP-N1-R"
                     /label="LGC_EGFP-N1-R"
                     /ApEinfo_label="LGC_EGFP-N1-R"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="#54a9a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1675..1691
                     /locus_tag="TTC31 mRNA"
                     /label="TTC31 mRNA"
                     /ApEinfo_label="TTC31 mRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1675..1691
                     /locus_tag="TTC31 CDS"
                     /label="TTC31 CDS"
                     /ApEinfo_label="TTC31 CDS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        1437..1488
                     /locus_tag="TTC31 RNA(1)"
                     /label="TTC31 RNA(1)"
                     /ApEinfo_label="TTC31 RNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        1675..1691
                     /locus_tag="TTC31 RNA(2)"
                     /label="TTC31 RNA(2)"
                     /ApEinfo_label="TTC31 RNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1385..1691
                     /locus_tag="TTC31 gene"
                     /label="TTC31 gene"
                     /ApEinfo_label="TTC31 gene"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#d20069"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1765..2481
                     /locus_tag="mEGFP"
                     /label="mEGFP"
                     /ApEinfo_label="mEGFP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3628..4635
                     /locus_tag="hygroB"
                     /label="hygroB"
                     /ApEinfo_label="hygroB"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="#d20069"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1759..1768
                     /locus_tag="Kozak sequence"
                     /label="Kozak sequence"
                     /ApEinfo_label="Kozak sequence"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(278..985)
                     /locus_tag="mCherry2"
                     /label="mCherry2"
                     /ApEinfo_label="mCherry2"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1698..1703
                     /locus_tag="KpnI"
                     /label="KpnI"
                     /ApEinfo_label="KpnI"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            complement(1058..1583)
                     /locus_tag="CCDC142 mRNA"
                     /label="CCDC142 mRNA"
                     /ApEinfo_label="CCDC142 mRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2482..2487
                     /locus_tag="SacI"
                     /label="SacI"
                     /ApEinfo_label="SacI"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="#ffff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(982..991)
                     /locus_tag="Kozak sequence(1)"
                     /label="Kozak sequence(1)"
                     /ApEinfo_label="Kozak sequence"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(1058..1583)
                     /locus_tag="CCDC142 gene"
                     /label="CCDC142 gene"
                     /ApEinfo_label="CCDC142 gene"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#d20069"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(992..1045)
                     /locus_tag="MyrPalm"
                     /label="MyrPalm"
                     /ApEinfo_label="MyrPalm"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff4aa5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1058..1190)
                     /locus_tag="CCDC142 CDS"
                     /label="CCDC142 CDS"
                     /ApEinfo_label="CCDC142 CDS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1449..1488
                     /locus_tag="TTC31 CDS(1)"
                     /label="TTC31 CDS(1)"
                     /ApEinfo_label="TTC31 CDS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff80c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1675..1691
                     /locus_tag="TTC31 mRNA(1)"
                     /label="TTC31 mRNA(1)"
                     /ApEinfo_label="TTC31 mRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1058..1076
                     /locus_tag="ccd_ttc promotor ForwP"
                     /label="ccd_ttc promotor ForwP"
                     /ApEinfo_label="ccd_ttc promotor ForwP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     gene            complement(6096..6956)
                     /locus_tag="Ampicillin"
                     /label="Ampicillin"
                     /ApEinfo_label="Ampicillin"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1058..1691
                     /locus_tag="source Homo sapiens"
                     /label="source Homo sapiens"
                     /ApEinfo_label="source Homo sapiens"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_RNA        1675..1691
                     /locus_tag="TTC31 RNA(3)"
                     /label="TTC31 RNA(3)"
                     /ApEinfo_label="TTC31 RNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2520..2747
                     /locus_tag="bGH_PA_terminator"
                     /label="bGH_PA_terminator"
                     /ApEinfo_label="bGH_PA_terminator"
                     /ApEinfo_fwdcolor="#ae0000"
                     /ApEinfo_revcolor="#804040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1385..1488
                     /locus_tag="TTC31 mRNA(2)"
                     /label="TTC31 mRNA(2)"
                     /ApEinfo_label="TTC31 mRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     mRNA            1426..1488
                     /locus_tag="TTC31 mRNA(3)"
                     /label="TTC31 mRNA(3)"
                     /ApEinfo_label="TTC31 mRNA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4844..4864)
                     /locus_tag="SV40 R"
                     /label="SV40 R"
                     /ApEinfo_label="SV40 R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     1645..1664
                     /locus_tag="P67 - seq 2D plus F"
                     /label="P67 - seq 2D plus F"
                     /ApEinfo_label="P67 - seq 2D plus F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     1059..1078
                     /locus_tag="P66 - seq 2D minus R"
                     /label="P66 - seq 2D minus R"
                     /ApEinfo_label="P66 - seq 2D minus R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     2495..2514
                     /locus_tag="P50 - BiDI seq (+) rev"
                     /label="P50 - BiDI seq (+) rev"
                     /ApEinfo_label="P50 - BiDI seq (+) rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     1097..1114
                     /locus_tag="P49 - BiDi seq (-) rev"
                     /label="P49 - BiDi seq (-) rev"
                     /ApEinfo_label="P49 - BiDi seq (-) rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     1628..1647
                     /locus_tag="P48 - BiDi seq (+) fw"
                     /label="P48 - BiDi seq (+) fw"
                     /ApEinfo_label="P48 - BiDi seq (+) fw"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(5307..5326)
                     /locus_tag="LGC_ASK-FN2"
                     /label="LGC_ASK-FN2"
                     /ApEinfo_label="LGC_ASK-FN2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4934..4952)
                     /locus_tag="LGC_Bluescript R"
                     /label="LGC_Bluescript R"
                     /ApEinfo_label="LGC_Bluescript R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4773..4795)
                     /locus_tag="LGC_EGFP-C1-R"
                     /label="LGC_EGFP-C1-R"
                     /ApEinfo_label="LGC_EGFP-C1-R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     110..129
                     /locus_tag="LGC_F1-oriR"
                     /label="LGC_F1-oriR"
                     /ApEinfo_label="LGC_F1-oriR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(2646..2665)
                     /locus_tag="LGC_F1-oriR(1)"
                     /label="LGC_F1-oriR(1)"
                     /ApEinfo_label="LGC_F1-oriR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4951..4971)
                     /locus_tag="LGC_M13-24R"
                     /label="LGC_M13-24R"
                     /ApEinfo_label="LGC_M13-24R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4926..4949)
                     /locus_tag="LGC-M13-24R-BLUE"
                     /label="LGC-M13-24R-BLUE"
                     /ApEinfo_label="LGC-M13-24R-BLUE"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4937..4954)
                     /locus_tag="LGC_M13-29R"
                     /label="LGC_M13-29R"
                     /ApEinfo_label="LGC_M13-29R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(5027..5046)
                     /locus_tag="LGC_M13rev2"
                     /label="LGC_M13rev2"
                     /ApEinfo_label="LGC_M13rev2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4999..5022)
                     /locus_tag="LGC_MKSF"
                     /label="LGC_MKSF"
                     /ApEinfo_label="LGC_MKSF"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     241..260
                     /locus_tag="LGC_pcDNA3.1-R"
                     /label="LGC_pcDNA3.1-R"
                     /ApEinfo_label="LGC_pcDNA3.1-R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(2515..2534)
                     /locus_tag="LGC_pcDNA3.1-R(1)"
                     /label="LGC_pcDNA3.1-R(1)"
                     /ApEinfo_label="LGC_pcDNA3.1-R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(4952..4971)
                     /locus_tag="LGC_pQE-F"
                     /label="LGC_pQE-F"
                     /ApEinfo_label="LGC_pQE-F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     7045..7064
                     /locus_tag="LGC_sCOS1F"
                     /label="LGC_sCOS1F"
                     /ApEinfo_label="LGC_sCOS1F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     4844..4864
                     /locus_tag="LGC_SV40"
                     /label="LGC_SV40"
                     /ApEinfo_label="LGC_SV40"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     3520..3537
                     /locus_tag="LGC_SV40-Do"
                     /label="LGC_SV40-Do"
                     /ApEinfo_label="LGC_SV40-Do"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     204..223
                     /locus_tag="LGC_UniV5his-R"
                     /label="LGC_UniV5his-R"
                     /ApEinfo_label="LGC_UniV5his-R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     primer_bind     complement(2552..2571)
                     /locus_tag="LGC_UniV5his-R(1)"
                     /label="LGC_UniV5his-R(1)"
                     /ApEinfo_label="LGC_UniV5his-R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     rep_origin      3415..3492
                     /locus_tag="SV40 origin"
                     /label="SV40 origin"
                     /ApEinfo_label="SV40 origin"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     rep_origin      2793..3221
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     misc_binding    complement(4960..4982)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(6998..7026)
                     /locus_tag="AmpR promotor"
                     /label="AmpR promotor"
                     /ApEinfo_label="AmpR promotor"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     terminator      complement(28..255)
                     /locus_tag="bGH PA terminator"
                     /label="bGH PA terminator"
                     /ApEinfo_label="bGH PA terminator"
                     /ApEinfo_fwdcolor="#ae0000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        3236..3451
                     /locus_tag="SV40 enhancer"
                     /label="SV40 enhancer"
                     /ApEinfo_label="SV40 enhancer"
                     /ApEinfo_fwdcolor="#51a8ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        3226..3595
                     /locus_tag="SV40 promotor"
                     /label="SV40 promotor"
                     /ApEinfo_label="SV40 promotor"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     promoter        4987..5016
                     /locus_tag="lac promotor"
                     /label="lac promotor"
                     /ApEinfo_label="lac promotor"
                     /ApEinfo_fwdcolor="#0069d2"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 1}
                     width 5 offset 0"
     polyA_signal    2523..2747
                     /locus_tag="bGH poly(A)signal"
                     /label="bGH poly(A)signal"
                     /ApEinfo_label="bGH poly(A)signal"
                     /ApEinfo_fwdcolor="#ffaeff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    complement(28..252)
                     /locus_tag="bGH poly(A)signal(1)"
                     /label="bGH poly(A)signal(1)"
                     /ApEinfo_label="bGH poly(A)signal"
                     /ApEinfo_fwdcolor="#ffaeff"
                     /ApEinfo_revcolor="#d5006a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    complement(5031..5052)
                     /locus_tag="CAP binding site"
                     /label="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="#004080"
                     /ApEinfo_revcolor="#004080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1046..1051
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    272..277
                     /locus_tag="EcoRI"
                     /label="EcoRI"
                     /ApEinfo_label="EcoRI"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#8080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gacggatcgg gagatctgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
       61 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
      121 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
      181 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
      241 tagaaggcac agtcgaggct gatcagcaat tgaattcCTT GTACAGCTCG TCCATGCCGC
      301 CGGTGGAGTG GCGGCCCTCG GCGCGTTCGT ACTGTTCCAC GATGGTGTAG TCCTCGTTGT
      361 GGGAAAGGAT GTCCAACTTG ATGTCGACGT TGTAGGCGCC GGGCAGCTGC ACGGGCTTCT
      421 TGGCCTTGTA GGTGGTCTTG ACCTCAGCGT CGTAGTGGCC GCCGTCCTTC AGCTTCAGCC
      481 TCTGCTTGAT CTCGCCCTTC AGGGCGCCGT CCTCGGGGTA CATCCGCTCA GTGGAGGCCT
      541 CCCAGCCCAT GGTACGACAC TGCATTACGG GGCCGTCGGA GGGGAAGTTG GTGCCGCGCA
      601 GCTTCACCTT GTAGATGAAC TCGCCGTCCT GCAGGGAGGA GTCCTGGGTC ACGGTCACCA
      661 CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCAATTGAA GCCCTCGGGG AAGGACAGCT
      721 TCAAGTAGTC GGGGATGTCG GCGGGGTGCT TCACGTAGGC CTTGGAGCCG TACATGAACT
      781 GAGGGGACAG GATGTCCCAG GCGAAGGGCA GGGGGCCACC CTTGGTCACC TTCAGCTTGG
      841 CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC CTCGATCTCG AACTCGTGGC
      901 CGTTCACGGA GCCCTCCATG TGCACCTTGA AGCGCATGAA CTCCTTGATG ATGGCCATGT
      961 TATCCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTTC ATCGTCATTG AGATTGTCTT
     1021 TCCTTTTTGA TTTAATACAT CCCATggatc cgattctagt gaacctccca gcgaagaccg
     1081 cccgttcgac ttctctccca ctgctcctcc ccagtgcccc cgggttgcgc cctcagcggg
     1141 ggcacgataa cgagtggagg caggctacct gagcgagacg cctgggccat ggggcggcgg
     1201 gtccagaacg aacctaacga ttcccacttc cctgcacgga ccaacgcccg ccgcagctcg
     1261 gacttcgccc catcgcaaga gccgttttct ccagtccggg agtcgcgggg accttcatgg
     1321 actctctcgt gctccgtaat gggaggcttc tgcccctaac aaacatggcc gcccactctg
     1381 tcccaccccg ccctttggct ggtatcaaat actagactac ctttcatttc cgggtcactg
     1441 tagatgcgat ggcgccgatt ccaaagactg tggggcggat caagctaggt gagcggtatg
     1501 acaagaccag tgggggtcta ggagggcaga ggcagcccct cttgggtcca cctgtggtct
     1561 gaacgtttcg agtaggattt tatgcagagg gcggagagtc gagggtagag tggggaggac
     1621 tctagcccat ctgtcccgag cccgctgacg aggctccgcc ttcctttcct gtagactgct
     1681 ctctacggcc ctagatcGGT ACCATGGGGA TGTATTAAAT CAAAAAGGAA AGACAATCTC
     1741 AATGACGATG AACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
     1801 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
     1861 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
     1921 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC
     1981 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
     2041 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
     2101 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
     2161 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT
     2221 ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC
     2281 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
     2341 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAG CAAAGACCCC
     2401 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
     2461 GGCATGGACG AGCTGTACAA GGAGCTCtag agggcccgtt taaacccgct gatcagcctc
     2521 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2581 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2641 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2701 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     2761 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     2821 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2881 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2941 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3001 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3061 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3121 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3181 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     3241 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3301 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3361 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3421 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3481 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     3541 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     3601 gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     3661 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     3721 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     3781 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     3841 gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     3901 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     3961 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     4021 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     4081 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     4141 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     4201 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     4261 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     4321 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     4381 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     4441 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     4501 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     4561 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     4621 ccgagggcaa aggaatagca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     4681 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4741 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     4801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4861 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     4921 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4981 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5041 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5101 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5161 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
     5881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5941 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6001 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6061 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7081 ccacctgacg tc    
//
