LOCUS       Exported               13235 bp ds-DNA     circular SYN 14-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Research Gerlich
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 10, 2022 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     792..808
                     /label=Primer 32
     LTR             834..1015
                     /gene="RU5"
                     /label=RU5
                     /note="/SECDrawAs=Region"
                     /note="/Transferred_From=backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=RIEPTETronly; RIKPB; RIKPH;
                     pHR-SFFV-dCas9-BFP-KRAB (modified); 
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP); 
                     pRRL.SFFV-rtTA3-IRES-EcoR-PGK-Puro (pRRL.RIEP) Copy"
                     /note="/X-Transferred_Similarity=100.00%"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    834..1014
                     /label=HIV-1_5_LTR
     misc_feature    834..1014
                     /label=truncHIV-1_3_LTR
     misc_feature    834..932
                     /label=R
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), 
                     lentiCas9-Hygro,pHR-SFFV-dCas9-BFP-KRAB 
                     (modified),pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);pLentiCRISPR-P2A-Thy1.1; 
                     plenti6gw_u6laminshrna_verB - R"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    835..1014
                     /gene="5'LTR"
                     /label=5'LTR
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), 
                     lentiCas9-Hygro,pHR-SFFV-dCas9-BFP-KRAB 
                     (modified),pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); m-m-pCDH-E - 5'LTR; 
                     pHR-SFFV-dCas9-BFP-KRAB(modified); pLentiCRISPR-P2A-Thy1.1"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    931..1015
                     /gene="U5"
                     /label=U5
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
                     /note="/Transferred_From=backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LUPG FKBP tTA P2A;
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    933..1014
                     /label=U5
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), lentiCas9-Hygro,lentiGuide-Puro
                     (modified), pHR-SFFV-dCas9-BFP-KRAB(modified), 
                     pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); lentiGuide-Puro 
                     (modified);pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);pLentiCRISPR-P2A-Thy1.1; 
                     plenti6gw_u6laminshrna_verB - U5"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1170..1534
                     /label=gag
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro, 
                     LentiCrisprmCherryP2Apuro-improved tracer, backup 
                     oflentiCas9-Hygro, lentiCRISPR v2 
                     (modified),lentiCas9-Blast (modified), lentiCas9-Hygro, 
                     lentiCrisprmCherryP2AneoR, lentiCrispr 
                     mCherryP2AneoR-improvedtracer, pLentiCRISPR, 
                     pLentiCRISPR-P2A-Thy1.1, pLentisgRNAThy1.1-P2A-neo
                     +Kosak, pLentisgRNA Thy1.1-P2A-neo +Kosak -improved 
                     backbone andkilled BsmBi site in thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro;LentiC
                     rispr mCherryP2Apuro-improved tracer; lentiCRISPR 
                     v2(modified); lentiCas9-Blast (modified); 
                     lentiCrisprmCherryP2AneoR; lentiCrispr 
                     mCherryP2AneoR-improvedtracer; pLentiCRISPR; 
                     pLentiCRISPR-P2A-Thy1.1; pLentisgRNAThy1.1-P2A-neo
                     +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak -improved 
                     backbone andkilled BsmBi site in 
                     thy1.1;pLentisgRNA-Thy1.1-P2A-Neo; 
                     pLentisgRNA-Thy1.1-P2A-NeoCopy"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    1584..1599
                     /label=SV40_int
                     /note="/Transferred_From=backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);
                     pLV-tTRKRAB (modified)"
                     /note="/X-Transferred_Similarity=100.00%; 68.75%"
                     /note="/X-Transferred_Translation=MSIQHFRVALIPFFAAFCLPVFAHP
                     ETLVK 
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ 
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL 
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ 
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT 
                     GSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     2131..2150
                     /label=Binding Region (2)
     misc_feature    2173..2186
                     /label=SV40_int
                     /note="/Transferred_From=backup of lentiCas9-Hygro, 
                     lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); pHR-SFFV-dCas9-BFP-KRAB 
                     (modified)"
                     /note="/X-Transferred_Similarity=100.00%; 81.25%"
                     /note="/X-Transferred_Translation=MSIQHFRVALIPFFAAFCLPVFAHP
                     ETLVK 
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ 
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL 
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ 
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT 
                     GSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    2416..2438
                     /label=HRP
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), lentiCas9-Hygro,lentiGuide-Puro
                     (modified), pHR-SFFV-dCas9-BFP-KRAB(modified), 
                     pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); lentiGuide-Puro 
                     (modified);pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);pLentiCRISPR-P2A-Thy1.1; plKO1 3xLac - HRP"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2439..2452
                     /label=cPPT
                     /note="/Transferred_From=backup of lentiCas9-Hygro, 
                     lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     lentiGuide-Puro (modified), pHR-SFFV-dCas9-BFP-KRAB 
                     (modified), pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiGuide-Puro (modified); 
                     pHR-SFFV-dCas9-BFP-KRAB (modified); 
                     pLentiCRISPR-P2A-Thy1.1"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    2443..2464
                     /label=U3PPT
                     /note="/Transferred_From=backup of lentiCas9-Hygro, 
                     lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro;LentiC
                     rispr mCherryP2Apuro-improved tracer; lentiCRISPR13450; 
                     lentiCRISPR v2 (modified); lentiCas9-Blast(modified); 
                     lentiCrispr mCherryP2AneoR; 
                     lentiCrisprmCherryP2AneoR-improved tracer; pCW-Cas9 
                     (modified);pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);pLentiCRISPR-P2A-Thy1.1; pLentisgRNA 
                     Thy1.1-P2A-neo+Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak - 
                     improvedbackbone andkilled BsmBi site in thy1.1"
                     /note="/X-Transferred_Similarity=100.00%; 86.36%"
                     /note="/X-Transferred_Translation=MLLLPVWLSFSPPCINPGCCLFMRS
                     CGPLS 
                     GNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWA 
                     LTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVF 
                     AFALRRVGSPFGPPPRIDTVDLEGINSSSVPLRPMTYKAAVDLSHFLKEKGGLEGLIH 
                     SQRRQDLLFACTGSLWLDQI*;MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWC 
                     ALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWC 
                     CRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRR 
                     VGSPFGPPPRIDTVDLET*;MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRG 
                     VVCTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNS 
                     VVLSGKLTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRP 
                     QTSRISLWAASPPVSPRRPD*"
     misc_feature    2443..2458
                     /label=cPPT
                     /note="/translation=MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAV
                     GIGAL 
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL 
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE 
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL 
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN 
                     GSALRAPILQTNGSIHPQF*"
     primer_bind     2466..2485
                     /label=Binding Region
     misc_feature    complement(2478..2521)
                     /label=R1 CRISPR
                     /note="/Transferred_From=backup of lentiCas9-Hygro, 
                     lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified), lentiCas9-Hygro,
                     lentiGuide-Puro (modified), pHR-SFFV-dCas9-BFP-KRAB 
                     (modified), pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiGuide-Puro (modified); 
                     pHR-SFFV-dCas9-BFP-KRAB (modified); 
                     pLentiCRISPR-P2A-Thy1.1"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(2478..2521)
                     /label=Binding Region (3)
     promoter        2649..2860
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     2802..2826
                     /label=Primer 35
     primer_bind     2824..2843
                     /label=Binding Region (4)
     primer_bind     2839..2862
                     /label=Primer 21
     primer_bind     2843..2868
                     /label=Primer 17
     regulatory      2875..2884
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2881..3597
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3505..3522
                     /label=Primer 25
     primer_bind     3528..3544
                     /label=Primer 27
     primer_bind     3559..3577
                     /label=Primer 23
     CDS             3607..3663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3647..3690
                     /label=AD74
                     /note="To insert stop codon at the very beginning of the 
                     sequence"
     primer_bind     3647..3684
                     /label=AD72
     CDS             3670..6537
                     /codon_start=1
                     /label=HAUS6
                     /translation="MSSASVTAFEKEHLWMYLQALGFEPGPATIACGKIVSHTHLGVNM
                     FDKLNRDAFHIISYFLFQVLDQSLTKEVFKFCWPPFDQKSDTEFRKHCCEWIKRISGEC
                     GSSFPQVVGSLFLSPGGPKFIHLMYHFARFVAMKYIKSNSKNSSHHFVETFNIKPQDLH
                     KCIARCHFARSRFLQILQRQDCVTQKYQENAQLSVKQVRNLRSECIGLENQIKKMEPYD
                     DHSNMEEKIQKVRSLWASVNETLMFLEKEREVVSSVLSLVNQYALDGTNVAINIPRLLL
                     DKIEKQMFQLHIGNVYEAGKLNLLTVIQLLNEVLKVMKYERCQADQARLTVDLHYLEKE
                     TKFQKERLSDLKHMRYRIKDDLTTIRHSVVEKQGEWHKKWKEFLGLSPFSLIKGWTPSV
                     DLLPPMSPLSFDPASEEVYAKSILCQYPASLPDAHKQHNQENGCRGDSDTLGALHDLAN
                     SPASFLSQSVSSSDRNSVTVLEKDTKMGTPKEKNEAISKKIPEFEVENSPLSDVAKNTE
                     SSAFGGSLPAKKSDPFQKEQDHLVEEVARAVLSDSPQLSEGKEIKLEELIDSLGSNPFL
                     TRNQIPRTPENLITEIRSSWRKAIEMEENRTKEPIQMDVEHREVLPESLPVLHNQREFS
                     MADFLLETTVSDFGQSHLTEEKVISDCECVPQKHVLTSHIDEPPTQNQSDLLNKKVICK
                     QDLECLAFTKLSETSRMETFSPAVGNRIDVMGGSEEEFMKILDHLEVSCNKPSTNKTML
                     WNSFQISIGISSKSFKDNDFGILHETLPEEVGHLSFNSSSSSEANFKLEPNSPMHGGTL
                     LEDVVGGRQTTPESDFNLQALRSRYEALKKSLSKKREESYLSNSQTPERHKPELSPTPQ
                     NVQTDDTLNFLDTCDLHTEHIKPSLRTSIGERKRSLSPLIKFSPVEQRLRTTIACSLGE
                     LPNLKEEDILNKSLDAKEPPSDLTR"
     primer_bind     complement(4257..4277)
                     /label=siRNA #218
     primer_bind     complement(6518..6557)
                     /label=AD73
     primer_bind     6550..6571
                     /label=Primer 17
     primer_bind     complement(6551..6576)
                     /label=Primer 22
     intron          6601..6830
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(6613..6629)
                     /label=Primer 24
     primer_bind     complement(6613..6629)
                     /label=Primer 14
     primer_bind     complement(6613..6629)
                     /label=Primer 13
     misc_feature    6903..7476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     7396..7412
                     /label=Primer 28
     primer_bind     7396..7412
                     /label=Primer 16
     primer_bind     7396..7412
                     /label=Primer 15
     primer_bind     7487..7528
                     /label=Primer 19
     primer_bind     complement(7488..7530)
                     /label=Primer 18
     CDS             7509..7907
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(7881..7934)
                     /label=Primer 20
     misc_feature    7927..8515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7979..8003)
                     /label=Primer 36
     primer_bind     complement(8002..8021)
                     /label=Binding Region (5)
     primer_bind     8288..8307
                     /label=Binding Region (6)
     CDS             complement(8398..8409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    8703..8718
                     /label=cPPT
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     LTR             8986..9221
                     /label=3' LTR SIN
                     /note="/Transferred_From=backup of lentiCas9-Hygro,
                     lentiCas9-Blast (modified), lentiCas9-Hygro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiGuide-Puro (modified); 
                     pLentiCRISPR; pLentiCRISPR-P2A-Thy1.1; pLentisgRNA 
                     Thy1.1-P2A-neo +Kosak; pLentisgRNA Thy1.1-P2A-neo +Kosak
                     - improved backbone andkilled BsmBi site in thy1.1"
                     /note="/X-Transferred_Similarity=100.00%; 86.44%"
     LTR             9040..9220
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9040..9220
                     /label=HIV-1_5_LTR
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    9040..9220
                     /label=truncHIV-1_3_LTR
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    9040..9138
                     /label=R
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), 
                     lentiCas9-Hygro,pHR-SFFV-dCas9-BFP-KRAB 
                     (modified),pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);pLentiCRISPR-P2A-Thy1.1; 
                     plenti6gw_u6laminshrna_verB - R"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    9041..9220
                     /gene="5'LTR"
                     /label=5'LTR
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), 
                     lentiCas9-Hygro,pHR-SFFV-dCas9-BFP-KRAB 
                     (modified),pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); m-m-pCDH-E - 5'LTR; 
                     pHR-SFFV-dCas9-BFP-KRAB(modified); pLentiCRISPR-P2A-Thy1.1"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    9137..9221
                     /gene="U5"
                     /label=U5
                     /note="/SECDrawAs=Region"
                     /note="/SECStyleId=1"
                     /note="/Transferred_From=backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LUPG FKBP tTA P2A;
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    9139..9220
                     /label=U5
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Blast (modified), lentiCas9-Hygro,lentiGuide-Puro
                     (modified), pHR-SFFV-dCas9-BFP-KRAB(modified), 
                     pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified);
                     lentiCas9-Blast(modified); lentiGuide-Puro 
                     (modified);pHR-SFFV-dCas9-BFP-KRAB 
                     (modified);pLentiCRISPR-P2A-Thy1.1; 
                     plenti6gw_u6laminshrna_verB - U5"
                     /note="/X-Transferred_Similarity=100.00%"
     CDS             9228..9459
                     /label=bGH polyA
                     /note="/Transferred_From=backup of lentiCas9-Hygro, 
                     lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified), lentiCas9-Hygro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=PX335 D10N PV; lentiCRISPR v2
                     (modified); lentiCas9-Blast (modified)"
                     /note="/X-Transferred_Similarity=100.00%; 97.41%"
     primer_bind     complement(9246..9263)
                     /label=Primer 31
     polyA_signal    9252..9476
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9441..9464)
                     /label=Binding Region (7)
     primer_bind     complement(9441..9459)
                     /label=Binding Region (7)
     rep_origin      9522..9977
                     /label=F1 ori
                     /note="/Transferred_From=backup of lentiCas9-Hygro, 
                     lentiCRISPR
                     v2 (modified), lentiCas9-Blast (modified), lentiCas9-Hygro"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=lentiCRISPR v2 (modified);
                     lentiCas9-Blast (modified); lentiGuide-Puro (modified); 
                     pLentiCRISPR; pLentisgRNA Thy1.1-P2A-neo +Kosak; 
                     pLentisgRNA Thy1.1-P2A-neo +Kosak - improved backbone 
                     andkilled BsmBi site in thy1.1"
                     /note="/X-Transferred_Similarity=100.00%; 95.18%"
     rep_origin      9522..9950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      9539..9845
                     /label=f1_origin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    9539..9845
                     /gene="f1 origin"
                     /label=f1 Origin
                     /label=f1 Origin; f1 origin
                     /note="/Description=Unknown feature type:Rep_Origin"
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Hygro, pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=GIPZ - RIEP-P - f1 origin; GIPZ 
                     - f1
                     origin; GIPZ - mcs1 - f1 origin; GIPZ-empty - f1 origin; 
                     GIPZ-empty2 - f1 origin; GIPZ_link(temp) - f1 origin; 
                     GIPZ_mcs - f1 origin; LentiCrisprmCherryP2Apuro-improved 
                     tracer; circular) - f1 Origin; circular)@1 - f1 Origin; 
                     lentiCRISPR v2 (modified); pCMV-SPORT6 (OBS; 
                     pCMV-SPORT6-Smyd2(640mut) - f1 Origin; 
                     pCMV-SPORT6-Smyd2(mouse) - f1 Origin; 
                     pCMV-SPORT6-Smyd2(mouse)-3'LINK - f1 Origin; 
                     pCMV-SPORT6-Smyd2(mouse)-LINK - f1 Origin; 
                     pCMV-SPORT6-Smyd2-3UTRLinker - f1 Origin; pGIPZ - EF1alpha-
                     rtTA3-IRES-Ametrine (SV40 and Hygro destroyed)
                     - f1 origin; pGIPZ - EF1alpha-tGFP-miR30-PGk-mCherry
                     (SV40 and Hygro destroyed) - f1 origin; pGIPZ - UbiC- 
                     rtTA3-IRES-Ametrine (SV40 and Hygro destroyed) - f1 origin;
                     pGIPZ - hCMVEF1alpha-rtTA3-IRES-Ametrine (SV40
                     and Hygro destroyed) - f1 origin; pGIPZ - hCMVSCEP- 
                     rtTA3-IRES-Ametrine (SV40 and Hygro destroyed) - f1 origin;
                     pGIPZ - rtTA3-IRES-Ametrine (SV40 and Hygro destroyed) - f1
                     origin; pGIPZ - tGFP-miR30 (SV40 and
                     Hygro destroyed) - f1 origin; pLentiCRISPR-P2A-Thy1.1"
                     /note="/X-Transferred_Similarity=100.00%"
     misc_feature    9542..9848
                     /label=f1 Ori
                     /label=f1 Ori; f1 ori
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro-improved
                     trace
                     r, backup of lentiCas9-Hygro, lentiCRISPR v2(modified), 
                     lentiCas9-Hygro, pLentiCRISPR-P2A-Thy1.1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro-improv
                     edtra
                     cer; lentiCRISPR v2 (modified); pCMV-MIR (Origene) - f1Ori;
                     pCMV-mIR150 - f1 Ori; pCMV-mIR34b - f1 Ori;pCMV-miR150 
                     (Origene) - f1 Ori; pCMV-miR21 (Origene)
                     - f1Ori; pCMV-miR29a (Origene) - f1 Ori; pCMV-miR34b 
                     (Origene)- f1 Ori; 
                     pKLO-CAGGS-CPPT-turboGFP-miR30_PGK-Neo-WRE(correct) - f1 
                     ori;pKLO-CAGGS-rtTA3ATGex-IRES-Ametrine-WRE - f1 
                     ori;pKLO-EF1alpha-turboGFP-miR30_PGK-Neo-WRE Mareike - f1 
                     ori;pKLO-IRES-Ametrine-WRE - f1 ori; 
                     pKLO-IRES-Neo-WRE(LINK-IN) - f1 
                     ori;pLKO-CAGGS-rtTA3-CPPT-IRES-Ametrine-WRE - f1 
                     ori;pLKO-CAGGS-rtTA3-IRES-Ametrine-WRE - f1 
                     ori;pLKO-CPPT-CAGGS-rtTA3-IRES-Ametrine-WRE - f1 
                     ori;pLKO-EF1alpha-rtTA3-IRES-Ametrine-WRE - f1 
                     ori;pLKO-NewLinker_Mareike - f1 
                     ori;pLKO-PGK-Cherry-IRES-Neo-WPRE_Mareike - f1 
                     ori;pLKO-PGK-Cherry-IRES-Neo_Mareike - f1 
                     ori;pLKO-TRE3G-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE
                     Mareike -f1 
                     ori;pLKO-TRE3G-GFP-miR30-cPPTCTS-PGK-Cherry-IRES-Neo-WPRE( 
                     L3alpha) Mareike - f1 
                     ori;pLKO-TRE3G-cPPTCTS-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE 
                     (L3beta XbaI linker + SpeI linker) Mareike - f1 
                     ori;pLKO-TRE3G-cPPTCTS-GFP-miR30-_PGK-Cherry-IRES-Neo-WPRE 
                     (L3beta XbaI linker) Mareike - f1 
                     ori;pLKO-TRE3G-turboGFP-miR30-cPPTCTS-PGK-Cherry-IRES-Neo- 
                     WPRE(L3alpha) Mareike - f1 
                     ori;pLKO-TRE3G-turboGFP-miR30_PGK-Cherry-IRES-Neo-WRE 
                     Mareike- f1 ori; pLKO-WREr_Mareike - f1 ori; 
                     pLKO.3G_Mareike - f1ori; pLentiCRISPR-P2A-Thy1.1; plKO1 
                     3xLac - f1 ori"
                     /note="/X-Transferred_Similarity=100.00%"
     primer_bind     9654..9674
                     /label=Binding Region (8)
     primer_bind     9798..9820
                     /label=Binding Region (9)
     promoter        9964..10293
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(9964..9981)
                     /label=Primer 33
     rep_origin      10144..10279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      10144..10221
                     /label=SV40_origin
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     misc_feature    10206..10225
                     /label=SV40pro_F_primer
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     promoter        10341..10388
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10407..10781
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            10407..10778
                     /gene="bleo"
                     /label=bleo
                     /note="/translation=MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALC
                     LLTQP 
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF 
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP 
                     PRIDTVDLET*"
     polyA_signal    10911..11032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10923..10940)
                     /label=Primer 34
     primer_bind     11016..11033
                     /label=Primer 30
     primer_bind     complement(11077..11092)
                     /label=Binding Region (10)
     primer_bind     complement(11081..11097)
                     /label=Binding Region; Binding Region; Binding Region;
                     Bi
     primer_bind     complement(11083..11099)
                     /label=Primer 26
     protein_bind    11174..11195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11483..12071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11530..11548)
                     /label=Binding Region (11)
     CDS             complement(12242..13102)
                     /label=AmpR
                     /label=AmpR; AmpR; ORF frame 1; ORF frame 2; ORF frame 3;
                     ORF frame 1; ORF frame 2; ORF frame 3
                     /note="/Transferred_From=LentiCrisprmCherryP2Apuro, 
                     LentiCrisprmCherryP2Apuro-improved tracer, backup 
                     oflentiCas9-Hygro, lentiCRISPR 13450, lentiCRISPR 
                     v2(modified), lentiCas9-Blast, lentiCas9-Hygro, 
                     lentiCrisprmCherryP2AneoR, lentiCrispr 
                     mCherryP2AneoR-improvedtracer, lentiGuide-Puro
                     (modified), pCW-Cas9 (modified),pLentiCRISPR"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/Transferred_Translation=MSIQHFRVALIPFFAAFCLPVFAHPET
                     LVKVK 
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG 
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA 
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI 
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS 
                     QATMDERNRQIAEIGASLIKHW*"
                     /note="/X-Transferred_From=LentiCrisprmCherryP2Apuro;LentiC
                     rispr mCherryP2Apuro-improved tracer; lentiCRISPR13450; 
                     lentiCRISPR v2 (modified); lentiCrisprmCherryP2AneoR; 
                     lentiCrispr mCherryP2AneoR-improvedtracer;
                     lentiGuide-Puro (modified); pCW-Cas9 
                     (modified);pLentiCRISPR"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/X-Transferred_Translation=MSIQHFRVALIPFFAAFCLPVFAHP
                     ETLVK 
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ 
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL 
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ 
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT 
                     GSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     13019..13036
                     /label=Primer 29
     promoter        complement(13103..13207)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    13143..13168
                     /label=LacO
                     /note="/Transferred_From=backup of lentiCas9-Hygro,
                     lentiCas9-Hygro, pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=LUPG FKBP tTA P2A;
                     pHR-SFFV-dCas9-BFP-KRAB (modified)"
                     /note="/X-Transferred_Similarity=100.00%; 65.38%"
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattag ctagctaggt cttgaaagga gtgggaattg gctccggtgc
     2641 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2701 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2761 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2821 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gagaattctc tagagccacc
     2881 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2941 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctat
     3001 ggtaagctta ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3061 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3121 cagcacgact ttttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3181 tttaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3241 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3301 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3361 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3421 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3481 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3541 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
     3601 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     3661 ccgaccggta tgagctcggc ctcggtcacc gctttcgaga aggagcatct ctggatgtat
     3721 ctgcaggcgc tcggcttcga gccaggcccg gcaaccattg cctgcggaaa gatcgtgtcg
     3781 cacacgcacc tcggagtgaa catgtttgac aagctgaacc gtgatgcctt tcatataatt
     3841 tcttattttt tgtttcaagt tctggaccag tctctcacca aagaagtttt caaattttgt
     3901 tggcccccat ttgaccaaaa aagtgacact gaattccgaa aacattgctg tgaatggata
     3961 aaaaggattt ctggtgaatg tggaagcagc tttcctcaag ttgttggttc actatttctt
     4021 tctcctggtg gtcctaagtt tattcatctg atgtatcatt ttgcaagatt tgttgcaatg
     4081 aaatatatta aatccaattc taaaaattct tctcatcatt ttgtagagac atttaacata
     4141 aaaccacagg acttgcacaa atgcattgcc agatgccatt tcgcacgtag cagattttta
     4201 caaattttgc aaagacaaga ttgtgttacc caaaaatatc aggaaaatgc acaattatca
     4261 gttaagcagg tacgaaactt gagatctgaa tgtataggat tggaaaacca aataaagaaa
     4321 atggaaccct atgatgacca cagtaatatg gaagaaaaaa ttcaaaaggt tcggtctttg
     4381 tgggcttcag tgaatgaaac gctcatgttt ttggaaaaag agagagaagt tgttagttcg
     4441 gtccttagtc ttgttaacca atatgcttta gatggaacta atgttgctat taatattcca
     4501 aggctcttac ttgacaaaat tgagaaacaa atgtttcagt tgcacatagg aaatgtttat
     4561 gaggctggaa aactgaacct cttaacagtt attcagttat taaatgaagt cttgaaggtg
     4621 atgaaatatg aacgttgtca ggctgatcaa gcaagattga cggtagacct tcactacctt
     4681 gaaaaagaga ccaaatttca gaaggaaaga ttatcagatc tgaaacatat gaggtataga
     4741 ataaaagatg atctcacaac tataagacat tctgttgttg aaaagcaagg agaatggcat
     4801 aaaaagtgga aagaatttct tggtttgtct cctttcagtc taattaaagg ttggactcca
     4861 tctgtagatc ttttaccacc aatgtctccc ctttcgtttg atcctgcctc agaagaagtg
     4921 tatgcaaaga gtattctttg tcagtatcct gcttcacttc cagatgcaca taagcaacat
     4981 aaccaagaaa atggttgcag aggagacagt gataccttgg gagcgctaca tgatctagcc
     5041 aacagccctg cctctttctt gtcgcagtca gtttcatcat cagatagaaa cagtgttaca
     5101 gtacttgaaa aggacacaaa gatgggaact cccaaagaaa aaaatgaagc aatttctaag
     5161 aaaataccag aatttgaagt ggaaaattct ccattatcag atgttgcaaa gaatacagag
     5221 agtagtgcat ttggagggtc tttgccagct aaaaaaagtg atccatttca aaaagagcaa
     5281 gatcatctgg tagaagaggt tgccagagca gttttatctg attcaccaca gctctctgaa
     5341 ggaaaagaaa taaaattaga ggaactaatt gactctctgg gttctaaccc cttcttaaca
     5401 aggaatcaga ttccccgtac tccagaaaac ttgataactg aaattaggag ctcatggaga
     5461 aaagctattg aaatggaaga aaacagaact aaagaaccaa ttcaaatgga tgttgaacat
     5521 agagaagtat tgccagaatc attacctgtg ttgcacaatc aaagagaatt tagcatggct
     5581 gattttctct tagaaaccac tgtatcagat tttggccagt ctcatttgac tgaagagaaa
     5641 gttatttcag attgcgagtg tgtgcctcag aaacatgtgc tgaccagtca catagatgaa
     5701 ccaccaacac aaaatcagtc agatttgtta aataagaaag taatttgcaa gcaagatttg
     5761 gaatgtttag ccttcaccaa gctttcagaa actagccgaa tggagacatt ctcccctgct
     5821 gtcggcaata ggatagatgt gatgggtggc agtgaagagg agtttatgaa aatattggac
     5881 cacttagaag tttcttgtaa caaaccttcc acaaataaaa ctatgttgtg gaattctttt
     5941 cagatatcaa ttggaattag ttctaagagt tttaaagata atgattttgg catattacac
     6001 gaaactctcc cggaagaagt tggtcatcta agttttaata gttccagtag ttcagaggcc
     6061 aattttaaac tggagccaaa tagtcctatg catggtggca ctcttctaga agatgttgtg
     6121 ggagggagac agactactcc agaatcagac tttaatttac aggctcttcg cagtagatac
     6181 gaggctctga agaaatctct ttccaagaaa agggaagaat cttacctctc gaattcccaa
     6241 acacccgaaa gacacaaacc agaattgagc cctactcccc aaaatgtaca aacagatgat
     6301 acgcttaact ttttggacac ctgtgatttg catactgagc atataaagcc atctttacgc
     6361 acgtccatcg gtgaaagaaa acggtctctt tcaccactaa ttaagttttc tccagtggaa
     6421 caaagattga gaaccacaat agcatgtagt cttggagaac tacctaattt aaaggaagaa
     6481 gacattttga ataagagcct tgatgcaaaa gaaccaccgt ctgacttgac aagatgagga
     6541 tccgaattcc gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
     6601 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
     6661 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
     6721 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
     6781 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
     6841 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc
     6901 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6961 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7021 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7081 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7141 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     7201 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     7261 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     7321 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7381 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7441 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac
     7501 gaccttccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     7561 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     7621 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     7681 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     7741 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     7801 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     7861 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaacga tatcaagctt
     7921 atcggtaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7981 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8041 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8101 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8161 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8221 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8281 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8341 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8401 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8461 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     8521 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     8581 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     8641 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8701 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     8761 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     8821 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     8881 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     8941 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     9001 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     9061 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9121 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9181 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     9241 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     9301 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     9361 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     9421 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     9481 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     9541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     9961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    10081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    10141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    10201 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    10261 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    10321 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
    10381 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
    10441 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
    10501 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
    10561 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
    10621 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
    10681 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
    10741 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
    10801 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    10861 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    10921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11041 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    11101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    11161 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    11221 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    11281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    11341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    11401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    11461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    11521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    11581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    11641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    11701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    12001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    12061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    12121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    12181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    12241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    12301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    12361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    12421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    12481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    12541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    12601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    12661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    12721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    12781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    12841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    12901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    12961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    13021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    13081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    13141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    13201 ttccgcgcac atttccccga aaagtgccac ctgac
//
