LOCUS       Exported                6526 bp ds-DNA     circular SYN 12-JUN-2015
DEFINITION  pH2B_mNeonGreen_FRB_IRESpuro2 rotated to position 6137.
ACCESSION   .
VERSION     .
KEYWORDS    1071_H2B_mNeonGreen_FRB_IRESpuro2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Vector molecule:  KI67_NO-LR-mNeonGreen-FRB*
               Fragment ends: BamHI and NotI
               Fragment size: 1034
            Insert molecule:  pH2B_Cherry_IRESpuro2*
               Fragment ends: NotI and BamHI
               Fragment size: 5492
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..390
                     /label=H2b
                     /note="H2b"
     CDS             13..1422
                     /codon_start=1
                     /label=H2B-mNeonGreen-FRB
                     /note="H2B-mNeonGreen-FRB"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAKDPVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQG
                     TGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQ
                     VHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKT
                     YPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSK
                     TELNFKEWQKAFTDVMGMDELYKTGGSSGSGGMASRILWHEMWHEGLEEASRLYFGERN
                     VKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWD
                     LYYHVFRRISK"
     CDS             397..1101
                     /gene="MKI67-mNeonGreen"
                     /label=MKI67-mNeonGreen
                     /note="mNeonGreen"
     CDS             1108..1419
                     /codon_start=1
                     /label=DmrC
                     /note="'DmrC"
                     /translation="GSSGSGGMASRILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPL
                     HAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK
                     "
     misc_feature    1108..1128
                     /label='Spacer
                     /note="'Spacer"
                     /note="Spacer"
                     /note="Spacer"
     misc_feature    1129..1419
                     /label=DmrC
                     /note="DmrC"
                     /note="DmrC = FRB (T2098L)"
                     /note="DmrC = FRB (T2098L)"
     misc_feature    1425..1455
                     /label='MCS
                     /note="'MCS"
     misc_feature    1455..1750
                     /label=IVS
                     /note="IVS"
     misc_feature    1776..2386
                     /label=IRES
                     /note="IRES"
     misc_feature    2387..2980
                     /label=Puro
                     /note="Puro"
     misc_feature    complement(4636..5567)
                     /label=Amp'
                     /note="Amp'"
     misc_feature    5941..6116
                     /label=CMV'
                     /note="CMV'"
ORIGIN
        1 ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa gggctccaag
       61 aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag ccgcaaggag
      121 agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac cggcatttcg
      181 tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg catcgcaggt
      241 gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag ggagatccag
      301 acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc cgagggtact
      361 aaggccatca ccaagtacac cagcgctaag gatccagtga gcaagggcga ggaggataac
      421 atggcctctc tcccagcgac acatgagtta cacatctttg gctccatcaa cggtgtggac
      481 tttgacatgg tgggtcaggg caccggcaat ccaaatgatg gttatgagga gttaaacctg
      541 aagtccacca agggtgacct ccagttctcc ccctggattc tggtccctca tatcgggtat
      601 ggcttccatc agtacctgcc ctaccctgac gggatgtcgc ctttccaggc cgccatggta
      661 gatggctccg gataccaagt ccatcgcaca atgcagtttg aagatggtgc ctcccttact
      721 gttaactacc gctacaccta cgagggaagc cacatcaaag gagaggccca ggtgaagggg
      781 actggtttcc ctgctgacgg tcctgtgatg accaactcgc tgaccgctgc ggactggtgc
      841 aggtcgaaga agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac
      901 accactggaa atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag
      961 ccaatggcgg ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc
     1021 aagcactcca agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg
     1081 ggcatggacg agctgtacaa gaccggtggc agctccggca gcggcggcat ggcttctaga
     1141 atcctctggc atgagatgtg gcatgaaggc ctggaagagg catctcgttt gtactttggg
     1201 gaaaggaacg tgaaaggcat gtttgaggtg ctggagccct tgcatgctat gatggaacgg
     1261 ggcccccaga ctctgaagga aacatccttt aatcaggcct atggtcgaga tttaatggag
     1321 gcccaagagt ggtgcaggaa gtacatgaaa tcagggaatg tcaaggacct cctccaagcc
     1381 tgggacctct attatcatgt gttccgacga atctcaaagt aagcggccgc atagataact
     1441 gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
     1501 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
     1561 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
     1621 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
     1681 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     1741 ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctccctcc ccccccccta
     1801 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgtgatttt
     1861 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     1921 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     1981 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     2041 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     2101 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     2161 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     2221 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     2281 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     2341 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc
     2401 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     2461 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     2521 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2581 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     2641 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     2701 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     2761 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     2821 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     2881 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa
     2941 ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     3001 gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc gacccgggcg
     3061 gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagagctcg ctgatcagcc
     3121 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3181 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3241 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3301 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3361 gaaagaacca gctggggctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3421 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     3481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3781 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3841 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3901 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3961 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4021 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     4081 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4141 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4201 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4261 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4321 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4381 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4441 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4501 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     5641 gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag
     5701 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     5761 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     5821 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     5881 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5941 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     6001 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6061 caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6121 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6181 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6241 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     6301 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     6361 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     6421 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
     6481 taccggactc agatctcgag ctcaagcttc gaattctgca gtcgac
//
