LOCUS       Exported               16356 bp ds-DNA     circular SYN 14-SEP-2022
DEFINITION  EGFP-Ki-67(RASA)-mCherry_correct rotated to position 9617.
ACCESSION   .
VERSION     .
KEYWORDS    1371_EGFP-Ki-67(RASA)-mCherry_correct
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Vector molecule:  EGFP-Ki67-mCherry_IRESpuro2*
               Fragment ends: EcoRV
               Fragment size: 11809
            Insert molecule:  KI67_RASA_long_mNeonGreen_IRESpuro2*
               Fragment ends: EcoRV
               Fragment size: 4547
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2..702
                     /gene="mEGFP"
                     /product="monomeric EGFP"
                     /label=mEGFP'
                     /note="monomeric EGFP"
                     /note="/dnas_title=""monomeric EGFP"""
     CDS             742..10506
                     /codon_start=1
                     /gene="MKI67"
                     /label='MKI67'''
                     /note="/translation=""MWPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDI
                      
                     RIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFR
                      
                     YENESLQNGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNP
                      
                     QVHIKNVKEDSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSR
                      
                     YGELKSVPTTQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATE
                      
                     KESADGLQGETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKA
                      
                     VGASFPLYEPAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLT
                      
                     PRKLSTRNRTPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLT
                      
                     QVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFG
                      
                     GHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIH
                      
                     VEVKAQSLVISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSK
                      
                     RVSISRSQHDILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMN
                      
                     KRQRRPATPKKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQK
                      
                     MDFKEDLSGIAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSES
                      
                     FGGNVFFSAQNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTET
                      
                     EPSKTVSTANRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEM
                      
                     KEIERPFETYKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTAR
                      
                     GQNLLQTQDHAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLA
                      
                     VGKFTRTSGETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSL
                      
                     EDLAGFKELFQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEE
                      
                     EFLALRKLTPSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEK
                      
                     AQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKA
                      
                     DVEGELLACRNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQT
                      
                     PKEEAQALEDLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTP
                      
                     LEKRDVQKELSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKR
                      
                     HPKTKEKAQPLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSK
                      
                     RSLRKVDVEEEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGS
                      
                     KRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSK
                      
                     RRLKTSLGKVGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAAS
                      
                     LTGSKRQLRTPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTP
                      
                     TSSKPQPKRSLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLD
                      
                     QPGNLPGSNRRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDP
                      
                     ADTPTNTKQRPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPV
                      
                     EKLDLLGNLPGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSP
                      
                     QPDPVKTPTSSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFK
                      
                     ESAKQMLDPANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIA
                      
                     CKSPPPESMDTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFR
                      
                     ETAKQKLDPAASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIA
                      
                     CRSPQPDPVGTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDM
                      
                     KAFMGTPVQKLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTT
                      
                     KIACKSPQPDPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDE
                      
                     KNINTFVETPVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTD
                      
                     DKITEVSCKSPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEP
                      
                     TGDSKSIKAFKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEE
                      
                     SMTIDKNTKIPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTH
                      
                     KEPASGDEGIKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHT
                      
                     QESLTAGKATKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTD
                      
                     ADKEPAGEDKGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGH
                      
                     TEESMTDDKTTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETT
                      
                     HTDKEPVGEGKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTP
                      
                     GHTEELANGAADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKS
                      
                     NTSLPPLPFKRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVV
                      
                     IMKRSLRTSAKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQI
                      
                     TEVFVLAERIEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNES
                      
                     SQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVT
                      RSVKRCAENPKKAEDNVCVKKIRTRSHRDSEDI"""
                     /translation="WPTRRLVTIKRSGVDGPHFPLSLSTCLFGRGIECDIRIQLPVVSK
                     QHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQNG
                     RKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNPQVHIKNVKE
                     DSTADDSKDSVAQGTTNVHSSEHAGRNGRNAADPISGDFKEISSVKLVSRYGELKSVPT
                     TQCLDNSKKNESPFWKLYESVKKELDVKSQKENVLQYCRKSGLQTDYATEKESADGLQG
                     ETQLLVSRKSRPKSGGSGHAVAEPASPEQELDQNKGKGRDVESVQTPSKAVGASFPLYE
                     PAKMKTPVQYSQQQNSPQKHKNKDLYTTGRRESVNLGKSEGFKAGDKTLTPRKLSTRNR
                     TPAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLTQVERKIQKD
                     SLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRASAGGHLRPELFD
                     ENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIKEQPQPSGKQESGSEIHVEVKAQSLV
                     ISPPAPSPRKTPVASDQRRRSCKTAPASSSKSQTEVPKRGGRKSGNLPSKRVSISRSQH
                     DILQMICSKRRSGASEANLIVAKSWADVVKLGAKQTQTKVIKHGPQRSMNKRQRRPATP
                     KKPVGEVHSQFSTGHANSPCTIIIGKAHTEKVHVPARPYRVLNNFISNQKMDFKEDLSG
                     IAEMFKTPVKEQPQLTSTCHIAISNSENLLGKQFQGTDSGEEPLLPTSESFGGNVFFSA
                     QNAAKQPSDKCSASPPLRRQCIRENGNVAKTPRNTYKMTSLETKTSDTETEPSKTVSTA
                     NRSGRSTEFRNIQKLPVESKSEETNTEIVECILKRGQKATLLQQRREGEMKEIERPFET
                     YKENIELKENDEKMKAMKRSRTWGQKCAPMSDLTDLKSLPDTELMKDTARGQNLLQTQD
                     HAKAPKSEKGKITKMPCQSLQPEPINTPTHTKQQLKASLGKVGVKEELLAVGKFTRTSG
                     ETTHTHREPAGDGKSIRTFKESPKQILDPAARVTGMKKWPRTPKEEAQSLEDLAGFKEL
                     FQTPGPSEESMTDEKTTKIACKSPPPESVDTPTSTKQWPKRSLRKADVEEEFLALRKLT
                     PSAGKAMLTPKPAGGDEKDIKAFMGTPVQKLDLAGTLPGSKRQLQTPKEKAQALEDLAG
                     FKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLAC
                     RNLMPSAGKAMHTPKPSVGEEKDIIIFVGTPVQKLDLTENLTGSKRRPQTPKEEAQALE
                     DLTGFKELFQTPGHTEEAVAAGKTTKMPCESSPPESADTPTSTRRQPKTPLEKRDVQKE
                     LSALKKLTQTSGETTHTDKVPGGEDKSINAFRETAKQKLDPAASVTGSKRHPKTKEKAQ
                     PLEDLAGLKELFQTPVCTDKPTTHEKTTKIACRSQPDPVDTPTSSKPQSKRSLRKVDVE
                     EEFFALRKRTPSAGKAMHTPKPAVSGEKNIYAFMGTPVQKLDLTENLTGSKRRLQTPKE
                     KAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKTSLGK
                     VGVKEELLAVGKLTQTSGETTHTHTEPTGDGKSMKAFMESPKQILDSAASLTGSKRQLR
                     TPKGKSEVPEDLAGFIELFQTPSHTKESMTNEKTTKVSYRASQPDLVDTPTSSKPQPKR
                     SLRKADTEEEFLAFRKQTPSAGKAMHTPKPAVGEEKDINTFLGTPVQKLDQPGNLPGSN
                     RRLQTRKEKAQALEELTGFRELFQTPCTDNPTTDEKTTKKILCKSPQSDPADTPTNTKQ
                     RPKRSLKKADVEEEFLAFRKLTPSAGKAMHTPKAAVGEEKDINTFVGTPVEKLDLLGNL
                     PGSKRRPQTPKEKAKALEDLAGFKELFQTPGHTEESMTDDKITEVSCKSPQPDPVKTPT
                     SSKQRLKISLGKVGVKEEVLPVGKLTQTSGKTTQTHRETAGDGKSIKAFKESAKQMLDP
                     ANYGTGMERWPRTPKEEAQSLEDLAGFKELFQTPDHTEESTTDDKTTKIACKSPPPESM
                     DTPTSTRRRPKTPLGKRDIVEELSALKQLTQTTHTDKVPGDEDKGINVFRETAKQKLDP
                     AASVTGSKRQPRTPKGKAQPLEDLAGLKELFQTPICTDKPTTHEKTTKIACRSPQPDPV
                     GTPTIFKPQSKRSLRKADVEEESLALRKRTPSVGKAMDTPKPAGGDEKDMKAFMGTPVQ
                     KLDLPGNLPGSKRWPQTPKEKAQALEDLAGFKELFQTPGTDKPTTDEKTTKIACKSPQP
                     DPVDTPASTKQRPKRNLRKADVEEEFLALRKRTPSAGKAMDTPKPAVSDEKNINTFVET
                     PVQKLDLLGNLPGSKRQPQTPKEKAEALEDLVGFKELFQTPGHTEESMTDDKITEVSCK
                     SPQPESFKTSRSSKQRLKIPLVKVDMKEEPLAVSKLTRTSGETTQTHTEPTGDSKSIKA
                     FKESPKQILDPAASVTGSRRQLRTRKEKARALEDLVDFKELFSAPGHTEESMTIDKNTK
                     IPCKSPPPELTDTATSTKRCPKTRPRKEVKEELSAVERLTQTSGQSTHTHKEPASGDEG
                     IKVLKQRAKKKPNPVEEEPSRRRPRAPKEKAQPLEDLAGFTELSETSGHTQESLTAGKA
                     TKIPCESPPLEVVDTTASTKRHLRTRVQKVQVKEEPSAVKFTQTSGETTDADKEPAGED
                     KGIKALKESAKQTPAPAASVTGSRRRPRAPRESAQAIEDLAGFKDPAAGHTEESMTDDK
                     TTKIPCKSSPELEDTATSSKRRPRTRAQKVEVKEELLAVGKLTQTSGETTHTDKEPVGE
                     GKGTKAFKQPAKRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGHTEELANG
                     AADSFTSAPKQTPDSGKPLKISRRVLRAPKVEPVGDVVSTRDPVKSQSKSNTSLPPLPF
                     KRGGGKDGSVTGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVVIMKRSLRTS
                     AKRIEPAEELNSNDMKTNKEEHKLQDSVPENKGISLRSRRQNKTEAEQQITEVFVLAER
                     IEINRNEKKPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSARQNESSQPKVAEES
                     GGQKSAKVLMQNQKGKGEAGNSDSMCLRSRKTKSQPAASTLESKSVQRVTRSVKRCAEN
                     PKKAEDNVCVKKIRTRSHRDSEDI"
     misc_feature    2251..2262
                     /label=RASA
     misc_feature    2255..2256
                     /label=RASA mutation 1
                     /note="TG -> CC mutation"
     misc_feature    2260..2261
                     /label=RASA mutation 2
                     /note="TT -> GC mutation"
     misc_feature    9772
                     /label=silent mutation 2
                     /note="C -> T mutation"
     CDS             10540..11250
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    11284..11579
                     /label=IVS
     misc_feature    11605..12215
                     /label=IRES
     misc_feature    12216..12809
                     /label=Puro
     misc_feature    complement(14465..15396)
                     /label=Amp'
     misc_feature    15770..16271
                     /label=CMV'
     misc_feature    16342..16347
                     /label=Kozak
                     /note="Kozak"
                     /note="Kozak"
     misc_feature    16343..16348
                     /gene="MCS"
                     /product="Multiple cloning sites"
                     /label=MCS
                     /note="Multiple cloning sites"
                     /note="/dnas_title=""Multiple cloning sites"""
ORIGIN
        1 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
       61 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      181 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      421 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      481 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      601 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctt
      661 aaggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcagc
      721 ggccccagcg gcagcagcag ctggcccacg agacgcctgg ttactatcaa aaggagcggg
      781 gtcgacggtc cccactttcc cctgagcctc agcacctgct tgtttggaag gggtattgaa
      841 tgtgacatcc gtatccagct tcctgttgtg tcaaaacaac attgcaaaat tgaaatccat
      901 gagcaggagg caatattaca taatttcagt tccacaaatc caacacaagt aaatgggtct
      961 gttattgatg agcctgtacg gctaaaacat ggagatgtaa taactattat tgatcgttcc
     1021 ttcaggtatg aaaatgaaag tcttcagaat ggaaggaagt caactgaatt tccaagaaaa
     1081 atacgtgaac aggagccagc acgtcgtgtc tcaagatcta gcttctcttc tgaccctgat
     1141 gagaaagctc aagattccaa ggcctattca aaaatcactg aaggaaaagt ttcaggaaat
     1201 cctcaggtac atatcaagaa tgtcaaagaa gacagtaccg cagatgactc aaaagacagt
     1261 gttgctcagg gaacaactaa tgttcattcc tcagaacatg ctggacgtaa tggcagaaat
     1321 gcagctgatc ccatttctgg ggattttaaa gaaatttcca gcgttaaatt agtgagccgt
     1381 tatggagaat tgaagtctgt tcccactaca caatgtcttg acaatagcaa aaaaaatgaa
     1441 tctccctttt ggaagcttta tgagtcagtg aagaaagagt tggatgtaaa atcacaaaaa
     1501 gaaaatgtcc tacagtattg tagaaaatct ggattacaaa ctgattacgc aacagagaaa
     1561 gaaagtgctg atggtttaca gggggagacc caactgttgg tctcgcgtaa gtcaagacca
     1621 aaatctggtg ggagcggcca cgctgtggca gagcctgctt cacctgaaca agagcttgac
     1681 cagaacaagg ggaagggaag agacgtggag tctgttcaga ctcccagcaa ggctgtgggc
     1741 gccagctttc ctctctatga gccggctaaa atgaagaccc ctgtacaata ttcacagcaa
     1801 caaaattctc cacaaaaaca taagaacaaa gacctgtata ctactggtag aagagaatct
     1861 gtgaatctgg gtaaaagtga aggcttcaag gctggtgata aaactcttac tcccaggaag
     1921 ctttcaacta gaaatcgaac accagctaaa gttgaagatg cagctgactc tgccactaag
     1981 ccagaaaatc tctcttccaa aaccagagga agtattccta cagatgtgga agttctgcct
     2041 acggaaactg aaattcacaa tgagccattt ttaactctgt ggctcactca agttgagagg
     2101 aagatccaaa aggattccct cagcaagcct gagaaattgg gcactacagc tggacagatg
     2161 tgctctgggt tacctggtct tagttcagtt gatatcaaca actttggtga ttccattaat
     2221 gagagtgagg gaataccttt gaaaagaagg cgtgcctccg ctggtgggca cctaagacct
     2281 gaactatttg atgaaaactt gcctcctaat acgcctctca aaaggggaga agccccaacc
     2341 aaaagaaagt ctctggtaat gcacactcca cctgtcctga agaaaatcat caaggaacag
     2401 cctcaaccat caggaaaaca agagtcaggt tcagaaatcc atgtggaagt gaaggcacaa
     2461 agcttggtta taagccctcc agctcctagt cctaggaaaa ctccagttgc cagtgatcaa
     2521 cgccgtaggt cctgcaaaac agcccctgct tccagcagca aatctcagac agaggttcct
     2581 aagagaggag ggagaaagag tggcaacctg ccttcaaaga gagtgtctat cagccgaagt
     2641 caacatgata ttttacagat gatatgttcc aaaagaagaa gtggtgcttc ggaagcaaat
     2701 ctgattgttg caaaatcatg ggcagatgta gtaaaacttg gtgcaaaaca aacacaaact
     2761 aaagtcataa aacatggtcc tcaaaggtca atgaacaaaa ggcaaagaag acctgctact
     2821 ccaaagaagc ctgtgggcga agttcacagt caatttagta caggccacgc aaactctcct
     2881 tgtaccataa taatagggaa agctcatact gaaaaagtac atgtgcctgc tcgaccctac
     2941 agagtgctca acaacttcat ttccaaccaa aaaatggact ttaaggaaga tctttcagga
     3001 atagctgaaa tgttcaagac cccagtgaag gagcaaccgc agttgacaag cacatgtcac
     3061 atcgctattt caaattcaga gaatttgctt ggaaaacagt ttcaaggaac tgattcagga
     3121 gaagaacctc tgctccccac ctcagagagt tttggaggaa atgtgttctt cagtgcacag
     3181 aatgcagcaa aacagccatc tgataaatgc tctgcaagcc ctcccttaag acggcagtgt
     3241 attagagaaa atggaaacgt agcaaaaacg cccaggaaca cctacaaaat gacttctctg
     3301 gagacaaaaa cttcagatac tgagacagag ccttcaaaaa cagtatccac tgcaaacagg
     3361 tcaggaaggt ctacagagtt caggaatata cagaagctac ctgtggaaag taagagtgaa
     3421 gaaacaaata cagaaattgt tgagtgcatc ctaaaaagag gtcagaaggc aacactacta
     3481 caacaaagga gagaaggaga gatgaaggaa atagaaagac cttttgagac atataaggaa
     3541 aatattgaat taaaagaaaa cgatgaaaag atgaaagcaa tgaagagatc aagaacttgg
     3601 gggcagaaat gtgcaccaat gtctgacctg acagacctca agagcttgcc tgatacagaa
     3661 ctcatgaaag acacggcacg tggccagaat ctcctccaaa cccaagatca tgccaaggca
     3721 ccaaagagtg agaaaggcaa aatcactaaa atgccctgcc agtcattaca accagaacca
     3781 ataaacaccc caacacacac aaaacaacag ttgaaggcat ccctggggaa agtaggtgtg
     3841 aaagaagagc tcctagcagt cggcaagttc acacggacgt caggggagac cacgcacacg
     3901 cacagagagc cagcaggaga tggcaagagc atcagaacgt ttaaggagtc tccaaagcag
     3961 atcctggacc cagcagcccg tgtaactgga atgaagaagt ggccaagaac gcctaaggaa
     4021 gaggcccagt cactagaaga cctggctggc ttcaaagagc tcttccagac accaggtccc
     4081 tctgaggaat caatgactga tgagaaaact accaaaatag cctgcaaatc tccaccacca
     4141 gaatcagtgg acactccaac aagcacaaag caatggccta agagaagtct caggaaagca
     4201 gatgtagagg aagaattctt agcactcagg aaactaacac catcagcagg gaaagccatg
     4261 cttacgccca aaccagcagg aggtgatgag aaagacatta aagcatttat gggaactcca
     4321 gtgcagaaac tggacctggc aggaacttta cctggcagca aaagacagct acagactcct
     4381 aaggaaaagg cccaggctct agaagacctg gctggcttta aagagctctt ccagactcct
     4441 ggtcacaccg aggaattagt ggctgctggt aaaaccacta aaataccctg cgactctcca
     4501 cagtcagacc cagtggacac cccaacaagc acaaagcaac gacccaagag aagtatcagg
     4561 aaagcagatg tagagggaga actcttagcg tgcaggaatc taatgccatc agcaggcaaa
     4621 gccatgcaca cgcctaaacc atcagtaggt gaagagaaag acatcatcat atttgtggga
     4681 actccagtgc agaaactgga cctgacagag aacttaaccg gcagcaagag acggccacaa
     4741 actcctaagg aagaggccca ggctctggaa gacctgactg gctttaaaga gctcttccag
     4801 acccctggtc atactgaaga agcagtggct gctggcaaaa ctactaaaat gccctgcgaa
     4861 tcttctccac cagaatcagc agacacccca acaagcacaa gaaggcagcc caagacacct
     4921 ttggagaaaa gggacgtaca gaaggagctc tcagccctga agaagctcac acagacatca
     4981 ggggaaacca cacacacaga taaagtacca ggaggtgagg ataaaagcat caacgcgttt
     5041 agggaaactg caaaacagaa actggaccca gcagcaagtg taactggtag caagaggcac
     5101 ccaaaaacta aggaaaaggc ccaaccccta gaagacctgg ctggcttgaa agagctcttc
     5161 cagacaccag tatgcactga caagcccacg actcacgaga aaactaccaa aatagcctgc
     5221 agatcacaac cagacccagt ggacacacca acaagctcca agccacagtc caagagaagt
     5281 ctcaggaaag tggacgtaga agaagaattc ttcgcactca ggaaacgaac accatcagca
     5341 ggcaaagcca tgcacacacc caaaccagca gtaagtggtg agaaaaacat ctacgcattt
     5401 atgggaactc cagtgcagaa actggacctg acagagaact taactggcag caagagacgg
     5461 ctacaaactc ctaaggaaaa ggcccaggct ctagaagacc tggctggctt taaagagctc
     5521 ttccagacac gaggtcacac tgaggaatca atgactaatg ataaaactgc caaagtagcc
     5581 tgcaaatctt cacaaccaga cccagacaaa aacccagcaa gctccaagcg acggctcaag
     5641 acatccctgg ggaaagtggg cgtgaaagaa gagctcctag cagttggcaa gctcacacag
     5701 acatcaggag agactacaca cacacacaca gagccaacag gagatggtaa gagcatgaaa
     5761 gcatttatgg agtctccaaa gcagatctta gactcagcag caagtctaac tggcagcaag
     5821 aggcagctga gaactcctaa gggaaagtct gaagtccctg aagacctggc cggcttcatc
     5881 gagctcttcc agacaccaag tcacactaag gaatcaatga ctaatgaaaa aactaccaaa
     5941 gtatcctaca gagcttcaca gccagaccta gtggacaccc caacaagctc caagccacag
     6001 cccaagagaa gtctcaggaa agcagacact gaagaagaat ttttagcatt taggaaacaa
     6061 acgccatcag caggcaaagc catgcacaca cccaaaccag cagtaggtga agagaaagac
     6121 atcaacacgt ttttgggaac tccagtgcag aaactggacc agccaggaaa tttacctggc
     6181 agcaatagac ggctacaaac tcgtaaggaa aaggcccagg ctctagaaga actgactggc
     6241 ttcagagagc ttttccagac accatgcact gataacccca cgactgatga gaaaactacc
     6301 aaaaaaatac tctgcaaatc tccgcaatca gacccagcgg acaccccaac aaacacaaag
     6361 caacggccca agagaagcct caagaaagca gacgtagagg aagaattttt agcattcagg
     6421 aaactaacac catcagcagg caaagccatg cacacgccta aagcagcagt aggtgaagag
     6481 aaagacatca acacatttgt ggggactcca gtggagaaac tggacctgct aggaaattta
     6541 cctggcagca agagacggcc acaaactcct aaagaaaagg ccaaggctct agaagatctg
     6601 gctggcttca aagagctctt ccagacacca ggtcacactg aggaatcaat gaccgatgac
     6661 aaaatcacag aagtatcctg caaatctcca caaccagacc cagtcaaaac cccaacaagc
     6721 tccaagcaac gactcaagat atccttgggg aaagtaggtg tgaaagaaga ggtcctacca
     6781 gtcggcaagc tcacacagac gtcagggaag accacacaga cacacagaga gacagcagga
     6841 gatggaaaga gcatcaaagc gtttaaggaa tctgcaaagc agatgctgga cccagcaaac
     6901 tatggaactg ggatggagag gtggccaaga acacctaagg aagaggccca atcactagaa
     6961 gacctggccg gcttcaaaga gctcttccag acaccagacc acactgagga atcaacaact
     7021 gatgacaaaa ctaccaaaat agcctgcaaa tctccaccac cagaatcaat ggacactcca
     7081 acaagcacaa ggaggcggcc caaaacacct ttggggaaaa gggatatagt ggaagagctc
     7141 tcagccctga agcagctcac acagaccaca cacacagaca aagtaccagg agatgaggat
     7201 aaaggcatca acgtgttcag ggaaactgca aaacagaaac tggacccagc agcaagtgta
     7261 actggtagca agaggcagcc aagaactcct aagggaaaag cccaacccct agaagacttg
     7321 gctggcttga aagagctctt ccagacacca atatgcactg acaagcccac gactcatgag
     7381 aaaactacca aaatagcctg cagatctcca caaccagacc cagtgggtac cccaacaatc
     7441 ttcaagccac agtccaagag aagtctcagg aaagcagacg tagaggaaga atccttagca
     7501 ctcaggaaac gaacaccatc agtagggaaa gctatggaca cacccaaacc agcaggaggt
     7561 gatgagaaag acatgaaagc atttatggga actccagtgc agaaattgga cctgccagga
     7621 aatttacctg gcagcaaaag atggccacaa actcctaagg aaaaggccca ggctctagaa
     7681 gacctggctg gcttcaaaga gctcttccag acaccaggca ctgacaagcc cacgactgat
     7741 gagaaaacta ccaaaatagc ctgcaaatct ccacaaccag acccagtgga caccccagca
     7801 agcacaaagc aacggcccaa gagaaacctc aggaaagcag acgtagagga agaattttta
     7861 gcactcagga aacgaacacc atcagcaggc aaagccatgg acacaccaaa accagcagta
     7921 agtgatgaga aaaatatcaa cacatttgtg gaaactccag tgcagaaact ggacctgcta
     7981 ggaaatttac ctggcagcaa gagacagcca cagactccta aggaaaaggc tgaggctcta
     8041 gaggacctgg ttggcttcaa agaactcttc cagacaccag gtcacactga ggaatcaatg
     8101 actgatgaca aaatcacaga agtatcctgt aaatctccac agccagagtc attcaaaacc
     8161 tcaagaagct ccaagcaaag gctcaagata cccctggtga aagtggacat gaaagaagag
     8221 cccctagcag tcagcaagct cacacggaca tcaggggaga ctacgcaaac acacacagag
     8281 ccaacaggag atagtaagag catcaaagcg tttaaggagt ctccaaagca gatcctggac
     8341 ccagcagcaa gtgtaactgg tagcaggagg cagctgagaa ctcgtaagga aaaggcccgt
     8401 gctctagaag acctggttga cttcaaagag ctcttctcag caccaggtca cactgaagag
     8461 tcaatgacta ttgacaaaaa cacaaaaatt ccctgcaaat ctcccccacc agaactaaca
     8521 gacactgcca cgagcacaaa gagatgcccc aagacacgtc ccaggaaaga agtaaaagag
     8581 gagctctcag cagttgagag gctcacgcaa acatcagggc aaagcacaca cacacacaaa
     8641 gaaccagcaa gcggtgatga gggcatcaaa gtattgaagc aacgtgcaaa gaagaaacca
     8701 aacccagtag aagaggaacc cagcaggaga aggccaagag cacctaagga aaaggcccaa
     8761 cccctggaag acctggccgg cttcacagag ctctctgaaa catcaggtca cactcaggaa
     8821 tcactgactg ctggcaaagc cactaaaata ccctgcgaat ctcccccact agaagtggta
     8881 gacaccacag caagcacaaa gaggcatctc aggacacgtg tgcagaaggt acaagtaaaa
     8941 gaagagcctt cagcagtcaa gttcacacaa acatcagggg aaaccacgga tgcagacaaa
     9001 gaaccagcag gtgaagataa aggcatcaaa gcattgaagg aatctgcaaa acagacaccg
     9061 gctccagcag caagtgtaac tggcagcagg agacggccaa gagcacccag ggaaagtgcc
     9121 caagccatag aagacctagc tggcttcaaa gacccagcag caggtcacac tgaagaatca
     9181 atgactgatg acaaaaccac taaaataccc tgcaaatcat caccagaact agaagacacc
     9241 gcaacaagct caaagagacg gcccaggaca cgtgcccaga aagtagaagt gaaggaggag
     9301 ctgttagcag ttggcaagct cacacaaacc tcaggggaga ccacgcacac cgacaaagag
     9361 ccggtaggtg agggcaaagg cacgaaagca tttaagcaac ctgcaaagcg gaagctggac
     9421 gcagaagatg taattggcag caggagacag ccaagagcac ctaaggaaaa ggcccaaccc
     9481 ctggaagatc tggccagctt ccaagagctc tctcaaacac caggccacac tgaggaactg
     9541 gcaaatggtg ctgctgatag ctttacaagc gctccaaagc aaacacctga cagtggaaaa
     9601 cctctaaaaa tatccagaag agttcttcgg gcccctaaag tagaacccgt gggagacgtg
     9661 gtaagcacca gagaccctgt aaaatcacaa agcaaaagca acacttccct gcccccactg
     9721 cccttcaaga ggggaggtgg caaagatgga agcgtcacgg gaaccaagag gttgcgctgc
     9781 atgccagcac cagaggaaat tgtggaggag ctgccagcca gcaagaagca gagggttgct
     9841 cccagggcaa gaggcaaatc atccgaaccc gtggtcatca tgaagagaag tttgaggact
     9901 tctgcaaaaa gaattgaacc tgcggaagag ctgaacagca acgacatgaa aaccaacaaa
     9961 gaggaacaca aattacaaga ctcggtccct gaaaataagg gaatatccct gcgctccaga
    10021 cgccaaaata agactgaggc agaacagcaa ataactgagg tctttgtatt agcagaaaga
    10081 atagaaataa acagaaatga aaagaagccc atgaagacct ccccagagat ggacattcag
    10141 aatccagatg atggagcccg gaaacccata cctagagaca aagtcactga gaacaaaagg
    10201 tgcttgaggt ctgctagaca gaatgagagc tcccagccta aggtggcaga ggagagcgga
    10261 gggcagaaga gtgcgaaggt tctcatgcag aatcagaaag ggaaaggaga agcaggaaat
    10321 tcagactcca tgtgcctgag atcaagaaag acaaaaagcc agcctgcagc aagcactttg
    10381 gagagcaaat ctgtgcagag agtaacgcgg agtgtcaaga ggtgtgcaga aaatccaaag
    10441 aaggctgagg acaatgtgtg tgtcaagaaa ataagaacca gaagtcatag ggacagtgaa
    10501 gatatttggg atccatcgtc gagcggtccc tcgggttcga tggtgagcaa gggcgaggag
    10561 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
    10621 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
    10681 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
    10741 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
    10801 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
    10861 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
    10921 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
    10981 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
    11041 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
    11101 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
    11161 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
    11221 accggcggca tggacgagct gtacaagtaa agcggccgca tagataactg atccagtgtg
    11281 ctggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctcaaaag
    11341 cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
    11401 cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct
    11461 ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact tgagtgacaa
    11521 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcaggtcga
    11581 gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa cgttactggc
    11641 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgtgattttc caccatattg
    11701 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
    11761 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
    11821 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
    11881 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
    11941 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
    12001 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
    12061 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
    12121 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
    12181 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
    12241 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
    12301 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
    12361 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
    12421 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
    12481 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
    12541 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
    12601 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
    12661 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
    12721 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga
    12781 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
    12841 ccgaaaggag cgcacgaccc catggctccg accgaagccg acccgggcgg ccccgccgac
    12901 cccgcacccg cccccgaggc ccaccgactc tagagctcgc tgatcagcct cgactgtgcc
    12961 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    13021 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    13081 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    13141 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
    13201 ctggggctcg agtgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    13261 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
    13321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    13381 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    13441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    13501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    13561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    13621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    13681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    13741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    13801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    13861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
    13921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    13981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    14041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    14101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    14161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    14221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    14281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    14341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    14401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    14461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    14521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    14581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    14641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    14701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    14761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    14821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    14881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    14941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    15001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    15061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    15121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    15181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    15241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    15301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    15361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    15421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
    15481 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    15541 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
    15601 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
    15661 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
    15721 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    15781 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    15841 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    15901 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    15961 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    16021 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
    16081 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
    16141 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    16201 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
    16261 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca
    16321 gatctcgagc tcaagcttcg aaccgccatg gctagc
//
