LOCUS       Exported                9593 bp ds-DNA     circular SYN 22-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Rha-RSW-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Pakrasi Lab WUSTL
  TITLE     Direct Submission
  JOURNAL   Exported Feb 10, 2022 from SnapGene 2.8.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          200..1036
                     /organism="Shuttle expression vector pCK306"
                     /mol_type="other DNA"
                     /db_xref="taxon:2162674"
     source          1337..1458
                     /organism="Shuttle expression vector pCK306"
                     /mol_type="other DNA"
                     /db_xref="taxon:2162674"
     misc_feature    1..199
                     /note="TrbcS"
     CDS             complement(200..1036)
                     /codon_start=1
                     /transl_table=11
                     /gene="rhaS"
                     /gene_synonym="ECK3898;JW3876;rhaC2"
                     /product="RhaS"
                     /note="rhaS"
                     /note="positive regulator for rhaBAD operon; 
                     transcriptional activator of rhaBAD and rhaT"
                     /db_xref="ASAP:ABE-0012745"
                     /db_xref="EcoGene:EG10843"
                     /db_xref="UniProtKB/Swiss-Prot:P09377"
                     /db_xref="GeneID:948397"
                     /protein_id="AVX33626.1"
                     /translation="MTVLHSVDFFPSGNVSVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     misc_feature    1037..1136
                     /note="PlacOI"
     gene            1037..1109
                     /pseudo
                     /locus_tag="AM397_15650"
                     /note="AM397_15650"
     CDS             1037..1109
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="AM397_15650"
                     /product="catalase"
                     /note="AM397_15650"
                     /note="incomplete; partial on complete genome; missing 
                     start; Derived by automated computational analysis using 
                     gene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /translation="AVSCVKLLSAHNSTQHTSRKHKV"
     protein_bind    1056..1072
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(1080..1110)
                     /note="Plac"
     misc_feature    1137..1336
                     /note="TpsbC"
     misc_feature    1337..1458
                     /note="rhamnose"
     misc_binding    1353..1368
                     /bound_moiety="CRP"
                     /note="CRP binding site"
     misc_binding    1372..1388
                     /bound_moiety="RhaS"
                     /note="RhaS operator 1"
                     /note="operator 1"
     misc_binding    1405..1420
                     /bound_moiety="RhaS"
                     /note="RhaS operator 2"
                     /note="operator 2"
     misc_feature    1418..1423
                     /note="PrhaBAD"
     misc_feature    1442..1447
                     /note="PrhaBAD"
     misc_feature    1459..1522
                     /note="RiboSwitch"
     misc_feature    1523..2456
                     /note="EYFP"
     misc_feature    1523..2245
                     /note="EYFP ORF"
     misc_feature    2246..2456
                     /note="TrrnB"
     misc_feature    2460..9590
                     /note="Plasmid backbone"
     CDS             complement(2516..3331)
                     /codon_start=1
                     /transl_table=11
                     /gene="aphA"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /function="kanamycin resistanse"
                     /note="aphA"
                     /db_xref="GI:4323384"
                     /protein_id="AAD16328.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4610..4894)
                     /codon_start=1
                     /transl_table=11
                     /gene="mobC"
                     /product="mobilization protein C"
                     /note="mobC"
                     /db_xref="GI:4323387"
                     /protein_id="AAD16331.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     CDS             5093..7222
                     /codon_start=1
                     /transl_table=11
                     /gene="mobA"
                     /product="mobilization protein A"
                     /note="mobA"
                     /db_xref="GI:4323385"
                     /protein_id="AAD16329.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     CDS             5841..6254
                     /codon_start=1
                     /transl_table=11
                     /gene="mobB"
                     /product="mobilization protein B"
                     /note="mobB"
                     /db_xref="GI:4323386"
                     /protein_id="AAD16330.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             6251..7222
                     /codon_start=1
                     /transl_table=11
                     /gene="repB"
                     /product="replication protein B"
                     /note="repB"
                     /db_xref="GI:4323389"
                     /protein_id="AAD16333.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             7283..7495
                     /codon_start=1
                     /transl_table=11
                     /product="unknown protein E"
                     /note="unknown protein E"
                     /db_xref="GI:4323392"
                     /protein_id="AAD16336.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             7497..7703
                     /codon_start=1
                     /transl_table=11
                     /gene="cac"
                     /product="repressor protein F"
                     /note="cac"
                     /db_xref="GI:4323391"
                     /protein_id="AAD16335.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             7733..8572
                     /codon_start=1
                     /transl_table=11
                     /gene="repA"
                     /product="replication protein A"
                     /note="repA"
                     /db_xref="GI:4323388"
                     /protein_id="AAD16332.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             8559..9410
                     /codon_start=1
                     /transl_table=11
                     /gene="repC"
                     /product="replication protein C"
                     /note="repC"
                     /db_xref="GI:4323390"
                     /protein_id="AAD16334.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
ORIGIN
        1 taattgacaa ttgacaattc cccacttaga taaaaaatcc ggtcaggatg cggtcaacat
       61 tatacaaaaa ctgtccaaag aacagggccg tattgtgttg ctgattacct atagtcactg
      121 tgaaaaagca acaaaaaacc cgcgggagcg ggctaacatt gaattgaagt cagtttttta
      181 gtaattgcca aaactgtaat tattgcagaa agccatcccg tccctgacga atatcacgcg
      241 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac
      301 agcgataggc gatgtcagta acgctggcct cactgtggcg tagcagatgt cgggctttca
      361 tcagtcgcag gcggttcagg tatcgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
      421 gatgtagcgt acgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
      481 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
      541 ggttctcctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aagatctcgc
      601 gactggcggt cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct
      661 gtcgcacctg ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca
      721 gctcttgtgg cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat
      781 acagcacatt ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc
      841 gtacgaaaca gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac
      901 ccgtgccatg ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg
      961 cctgcgggag ccggggttct atcgccacgg acacgttacc agacggaaaa aaatccacac
     1021 tatgtaatac ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaattg
     1141 agacttttct gattttgcaa aggttttgct ttagttaaac ccaattgatt agtgtcccct
     1201 gcccatttgg tgggggatta ttatttttaa gataatccta ttttttggag tgaggccagt
     1261 tacctattag acgcgcgact cgaaagtcgt tcaggggagt tggaacggct tccaaaaacc
     1321 tttccccgct ggtgttccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
     1381 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt
     1441 tagactggtc gtaatgaata ccggtgatac cagcatcgtc ttgatgccct tggcagcacc
     1501 ctgctaagga ggtaacaaca agatggtgag caagggcgag gagctgttca ccggggtggt
     1561 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1621 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1681 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
     1741 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta
     1801 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1861 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1921 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1981 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     2041 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2101 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     2161 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2221 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     2281 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2341 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagta gcggccgctg
     2401 cagtccggca aaaaaacggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgtgt
     2461 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
     2521 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     2581 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     2641 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     2701 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     2761 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     2821 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     2881 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     2941 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     3001 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     3061 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     3121 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     3181 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     3241 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg
     3301 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     3361 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     3421 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     3481 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     3541 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     3601 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
     3661 gctattctga ccttgccatc acgactgtgc tggtcattaa acgcgtattc aggctgaccc
     3721 tgcgcgctgc gcagggcttt attgattcca tttttacact gatgaatgtt ccgttgcgct
     3781 gcccggatta cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg
     3841 tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata
     3901 gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc
     3961 aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg
     4021 atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg
     4081 tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg
     4141 ttgctagaca ttgccagcca gtgcccgtca ctcccgtact aactgtcacg aacccctgca
     4201 ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc
     4261 caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga
     4321 ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct
     4381 tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca
     4441 ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa
     4501 accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga
     4561 agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct
     4621 catccttctg gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg
     4681 catccattgc cgccatgagc cgatcctccg gccactcgct gctgttcacc ttggccaaaa
     4741 tcatggcccc caccagcacc ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc
     4801 ttgcccgcac ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg agcttggcca
     4861 gccgatccgc cgccttgttg ctccccttaa ccatcttgac accccattgt taatgtgctg
     4921 tctcgtaggc tatcatggag gcacagcggc ggcaatcccg accctacttt gtaggggagg
     4981 gcgcacttac cggtttctct tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc
     5041 tctccgaggg ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat
     5101 ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc caatcggcca gggccaaggc
     5161 cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga
     5221 atccgggcac atgccggagt tcgtcgagcg gcccgccgac tactgggatg ctgccgacct
     5281 gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct
     5341 gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc cagcacctga ccggtgccga
     5401 gcgcctgccg tatacgctgg ccatccatgc cggtggcggc gagaacccgc actgccacct
     5461 gatgatctcc gagcggatca atgacggcat cgagcggccc gccgctcagt ggttcaagcg
     5521 gtacaacggc aagaccccgg agaagggcgg ggcacagaag accgaagcgc tcaagcccaa
     5581 ggcatggctt gagcagaccc gcgaggcatg ggccgaccat gccaaccggg cattagagcg
     5641 ggctggccac gacgcccgca ttgaccacag aacacttgag gcgcagggca tcgagcgcct
     5701 gcccggtgtt cacctggggc cgaacgtggt ggagatggaa ggccggggca tccgcaccga
     5761 ccgggcagac gtggccctga acatcgacac cgccaacgcc cagatcatcg acttacagga
     5821 ataccgggag gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc agaggcatca
     5881 acgagttagc ggagcagatc gaaccgctgg cccagagcat ggcgacactg gccgacgaag
     5941 cccggcaggt catgagccag acccagcagg ccagcgaggc gcaggcggcg gagtggctga
     6001 aagcccagcg ccagacaggg gcggcatggg tggagctggc caaagagttg cgggaggtag
     6061 ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg tggcactgga
     6121 agctatggct aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat
     6181 cgttgctctt gctcgacctg acgccactga caaccgagga cggctcgatc tggctgcgct
     6241 tggtggcccg atgaagaacg acaggacttt gcaggccata ggccgacagc tcaaggccat
     6301 gggctgtgag cgcttcgata tcggcgtcag ggacgccacc accggccaga tgatgaaccg
     6361 ggaatggtca gccgccgaag tgctccagaa cacgccatgg ctcaagcgga tgaatgccca
     6421 gggcaatgac gtgtatatca ggcccgccga gcaggagcgg catggtctgg tgctggtgga
     6481 cgacctcagc gagtttgacc tggatgacat gaaagccgag ggccgggagc ctgccctggt
     6541 agtggaaacc agcccgaaga actatcaggc atgggtcaag gtggccgacg ccgcaggcgg
     6601 tgaacttcgg gggcagattg cccggacgct ggccagcgag tacgacgccg acccggccag
     6661 cgccgacagc cgccactatg gccgcttggc gggcttcacc aaccgcaagg acaagcacac
     6721 cacccgcgcc ggttatcagc cgtgggtgct gctgcgtgaa tccaagggca agaccgccac
     6781 cgctggcccg gcgctggtgc agcaggctgg ccagcagatc gagcaggccc agcggcagca
     6841 ggagaaggcc cgcaggctgg ccagcctcga actgcccgag cggcagctta gccgccaccg
     6901 gcgcacggcg ctggacgagt accgcagcga gatggccggg ctggtcaagc gcttcggtga
     6961 tgacctcagc aagtgcgact ttatcgccgc gcagaagctg gccagccggg gccgcagtgc
     7021 cgaggaaatc ggcaaggcca tggccgaggc cagcccagcg ctggcagagc gcaagcccgg
     7081 ccacgaagcg gattacatcg agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca
     7141 gcttgcgcgg gccgagctgg cacgggcacc ggcaccccgc cagcgaggca tggacagggg
     7201 cgggccagat ttcagcatgt agtgcttgcg ttggtactca cgcctgttat actatgagta
     7261 ctcacgcaca gaagggggtt ttatggaata cgaaaaaagc gcttcagggt cggtctacct
     7321 gatcaaaagt gacaagggct attggttgcc cggtggcttt ggttatacgt caaacaaggc
     7381 cgaggctggc cgcttttcag tcgctgatat ggccagcctt aaccttgacg gctgcacctt
     7441 gtccttgttc cgcgaagaca agcctttcgg ccccggcaag tttctcggtg actgatatga
     7501 aagaccaaaa ggacaagcag accggcgacc tgctggccag ccctgacgct gtacgccaag
     7561 cgcgatatgc cgagcgcatg aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg
     7621 acgacgaata cgaggcgctg cgcgagtgcc tggaagaact cagagcggcg cagggcgggg
     7681 gtagtgaccc cgccagcgcc taaccaccaa ctgcctgcaa aggaggcaat caatggctac
     7741 ccataagcct atcaatattc tggaggcgtt cgcagcagcg ccgccaccgc tggactacgt
     7801 tttgcccaac atggtggccg gtacggtcgg ggcgctggtg tcgcccggtg gtgccggtaa
     7861 atccatgctg gccctgcaac tggccgcaca gattgcaggc gggccggatc tgctggaggt
     7921 gggcgaactg cccaccggcc cggtgatcta cctgcccgcc gaagacccgc ccaccgccat
     7981 tcatcaccgc ctgcacgccc ttggggcgca cctcagcgcc gaggaacggc aagccgtggc
     8041 tgacggcctg ctgatccagc cgctgatcgg cagcctgccc aacatcatgg ccccggagtg
     8101 gttcgacggc ctcaagcgcg ccgccgaggg ccgccgcctg atggtgctgg acacgctgcg
     8161 ccggttccac atcgaggaag aaaacgccag cggccccatg gcccaggtca tcggtcgcat
     8221 ggaggccatc gccgccgata ccgggtgctc tatcgtgttc ctgcaccatg ccagcaaggg
     8281 cgcggccatg atgggcgcag gcgaccagca gcaggccagc cggggcagct cggtactggt
     8341 cgataacatc cgctggcagt cctacctgtc gagcatgacc agcgccgagg ccgaggaatg
     8401 gggtgtggac gacgaccagc gccggttctt cgtccgcttc ggtgtgagca aggccaacta
     8461 tggcgcaccg ttcgctgatc ggtggttcag gcggcatgac ggcggggtgc tcaagcccgc
     8521 cgtgctggag aggcagcgca agagcaaggg ggtgccccgt ggtgaagcct aagaacaagc
     8581 acagcctcag ccacgtccgg cacgacccgg cgcactgtct ggcccccggc ctgttccgtg
     8641 ccctcaagcg gggcgagcgc aagcgcagca agctggacgt gacgtatgac tacggcgacg
     8701 gcaagcggat cgagttcagc ggcccggagc cgctgggcgc tgatgatctg cgcatcctgc
     8761 aagggctggt ggccatggct gggcctaatg gcctagtgct tggcccggaa cccaagaccg
     8821 aaggcggacg gcagctccgg ctgttcctgg aacccaagtg ggaggccgtc accgctgaat
     8881 gccatgtggt caaaggtagc tatcgggcgc tggcaaagga aatcggggca gaggtcgata
     8941 gtggtggggc gctcaagcac atacaggact gcatcgagcg cctttggaag gtatccatca
     9001 tcgcccagaa tggccgcaag cggcaggggt ttcggctgct gtcggagtac gccagcgacg
     9061 aggcggacgg gcgcctgtac gtggccctga accccttgat cgcgcaggcc gtcatgggtg
     9121 gcggccagca tgtgcgcatc agcatggacg aggtgcgggc gctggacagc gaaaccgccc
     9181 gcctgctgca ccagcggctg tgtggctgga tcgaccccgg caaaaccggc aaggcttcca
     9241 tagatacctt gtgcggctat gtctggccgt cagaggccag tggttcgacc atgcgcaagc
     9301 gccgccagcg ggtgcgcgag gcgttgccgg agctggtcgc gctgggctgg acggtaaccg
     9361 agttcgcggc gggcaagtac gacatcaccc ggcccaaggc ggcaggctga ccccccccac
     9421 tctattgtaa acaagacatt tttatctttt atattcaatg gcttattttc ctgctaattg
     9481 gtaataccat gaaaaatacc atgctcagaa aaggcttaac aatattttga aaaattgcct
     9541 actgagcgct gccgcacagc tccataggcc gctttcctgg ctttgcttcc cac
//
