LOCUS Exported 13855 bp ds-DNA circular SYN 02-NOV-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSL2796-pJU115 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13855) AUTHORS Pakrasi Lab WUSTL TITLE Direct Submission JOURNAL Exported Feb 10, 2022 from SnapGene 2.8.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13855 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(394..1209) /codon_start=1 /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 2067..2461 /note="RSF1010 oriV" /note="replication origin of the broad-host-range plasmid RSF1010 from Salmonella enterica serovar Typhimurium; requires the RSF1010 RepA/B/C proteins for replication" misc_feature 2488..2772 /note="mobC" misc_feature 2803..2890 /note="RSF1010 oriT" misc_feature 2971..5100 /note="mobA" CDS 4129..5100 /codon_start=1 /gene="repB" /product="replication protein B of the broad-host-range plasmid RSF1010 from Salmonella enterica serovar Typhimurium" /note="RSF1010 RepB" /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM" CDS 5611..6450 /codon_start=1 /gene="repA" /product="replication protein A of the broad-host-range plasmid RSF1010 from Salmonella enterica serovar Typhimurium" /note="RSF1010 RepA" /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA" CDS 6437..7288 /codon_start=1 /gene="repC" /product="replication protein C of the broad-host-range plasmid RSF1010 from Salmonella enterica serovar Typhimurium" /note="RSF1010 RepC" /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA AG" promoter 7921..7950 /note="lac promoter" /note="promoter for the E. coli lac operon" CDS 7965..11867 /codon_start=1 /note="cpf1" /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL VIKNEEYFEFVQNRNN" promoter 11874..11908 /note="J23119 promoter" /note="bacterial promoter (Registry of Standard Biological Parts BBa_J23119)" misc_feature 11929..11960 /note="Direct repeat" misc_feature 11985..12020 /note="Direct repeat" misc_feature 12051..12086 /note="Direct repeat" misc_feature 12114..12149 /note="direct repeat" CDS 12322..13232 /codon_start=1 /note="psbA1" /translation="DIDGIREPVAGSLMYGNNIISGAVVPSSNAIGLHFYPIWEAASLD EWLYNGGPYQLVVFHFLLGISCYMGRQWELSYRLGMRPWICVAYSAPLSAAFAVFLIYP IGQGSFSDGMPLGISGTFNFMFVFQAEHNILMHPFHMLGVAGVFGGSLFSAMHGSLVTS SLVRETTETESQNYGYKFGQEEETYNIVAAHGYFGRLIFQYAAFNNSRSLHFFLGAWPV VGIWFTSMGISTMAFNLNGFNFNQSVLDSQGKVINTWADVLNRANLGMEVMHERNAHNF PLDLAAGEATPVALTAPSIHG" misc_feature 12908..12910 /note="mutated PAM - silent mutation" misc_feature 12911..12930 /note="cpf1 target" misc_feature 12939 /note="S264A Point mutation" misc_feature 12940..12941 /note="Ser 264" ORIGIN 1 cattcatcag tgtaaaaatg gaatcaataa agccctgcgc agcgcgcagg gtcagcctga 61 atacgcgttt aatgaccagc acagtcgtga tggcaaggtc agaatagcgc tgaggtctgc 121 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga 181 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga 241 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca 301 actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct 361 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg 421 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg 481 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc 541 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag 601 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt 661 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact 721 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc 781 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag 841 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt 901 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat 961 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc 1021 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata 1081 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata 1141 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat 1201 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga 1261 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg 1321 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc 1381 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc 1441 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta 1501 tgagtcagca acaccttctt cacgaggcag acctcagcgc tattctgacc ttgccatcac 1561 gactgtgctg gtcattaaac gcgtattcag gctgaccctg cgcgctgcgc agggctttat 1621 tgattccatt tttacactga tgaatgttcc gttgcgctgc ccggattaca gctgaaagcg 1681 accaggtgct cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc 1741 ccgcattgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc 1801 tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt 1861 tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc 1921 gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta 1981 gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt 2041 gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg cccccctgca 2101 ataactgtca cgaacccctg caataactgt cacgccccca aacctgcaaa cccagcaggg 2161 gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt atctaagaat aatccactag 2221 gcgcggttat cagcgccctt gtggggcgct gctgcccttg cccaatatgc ccggccagag 2281 gccggatagc tggtctattc gctgcgctag gctacacacc gccccaccgc tgcgcggcag 2341 ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac cccgcagaaa tacgctggag 2401 cgcttttagc cgctttagcg gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc 2461 cccgcagggc ctgtctcggt cgatcattca gcccggctca tccttctggc gtggcggcag 2521 accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca tccattgccg ccatgagccg 2581 atcctccggc cactcgctgc tgttcacctt ggccaaaatc atggccccca ccagcacctt 2641 gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt gcccgcaccc gctgaatttc 2701 ggcattgatt cgcgctcgtt gttcttcgag cttggccagc cgatccgccg ccttgttgct 2761 ccccttaacc atcttgacac cccattgtta atgtgctgtc tcgtaggcta tcatggaggc 2821 acagcggcgg caatcccgac cctactttgt aggggagggc gcacttaccg gtttctcttc 2881 gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc attgcatgga 2941 gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac ggcgaaaacc 3001 ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca gcgcgaaggc 3061 aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat gccggagttc 3121 gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc caatgggcgg 3181 ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca gcagaaggcg 3241 ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta tacgctggcc 3301 atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga gcggatcaat 3361 gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag 3421 aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc 3481 gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga cgcccgcatt 3541 gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg 3601 aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac 3661 atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat 3721 gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga 3781 accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac 3841 ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc 3901 ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc 3961 gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct 4021 ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac 4081 gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac 4141 aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc 4201 ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg 4261 ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg 4321 cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg 4381 gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac 4441 tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc 4501 cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc 4561 cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg 4621 tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag 4681 caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc 4741 agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac 4801 cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt 4861 atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg 4921 gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag 4981 cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca 5041 cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag 5101 tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt 5161 atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat 5221 tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc 5281 gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag 5341 cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac 5401 cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa 5461 ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg 5521 cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta 5581 accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg 5641 gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt 5701 acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg 5761 gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg 5821 gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt 5881 ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg 5941 ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc 6001 gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa 6061 aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc 6121 gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc 6181 gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc 6241 tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc 6301 cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg 6361 tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag 6421 agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca 6481 cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa 6541 gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg 6601 cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg 6661 gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct 6721 gttcctggaa cccaagtggg aggccgtcac cgctgaatgc catgtggtca aaggtagcta 6781 tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat 6841 acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg 6901 gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt 6961 ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag 7021 catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg 7081 tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt 7141 ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc 7201 gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga 7261 catcacccgg cccaaggcgg caggctgacc ccccccactc tattgtaaac aagacatttt 7321 tatcttttat attcaatggc ttattttcct gctaattggt aataccatga aaaataccat 7381 gctcagaaaa ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc 7441 cataggccgc tttcctggct ttgcttccag atgtatgctc ttctgctcct gcaggcatcg 7501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 7561 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 7801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7921 tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatgtca atttatcaag 7981 aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc ccacagggta 8041 aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa agagctaaag 8101 actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata gaggagatat 8161 taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt tattttaaac 8221 ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa gatacgataa 8281 agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg tttaatcaaa 8341 accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta aagcaatcta 8401 aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata gatgaggcgt 8461 tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt catgaaaata 8521 gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg atagtagatg 8581 ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa gacaaagctc 8641 cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta acctttgata 8701 ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa gtttttgaga 8761 tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat actattattg 8821 gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa tatataaatc 8881 tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt gttttattta 8941 agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta gaagatgata 9001 gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt aaaacagtag 9061 aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa gctcaaaaac 9121 ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta tcacaacaag 9181 tttttgatga ttatagtgtt attggtacag cggtactaga atatataact caacaaatag 9241 cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc aaaaaaactg 9301 aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa tttaataagc 9361 atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt gcggctattc 9421 cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata tctatcaaat 9481 atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat gttaaagcta 9541 tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata tttcatatta 9601 gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat ctagtatttg 9661 aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt agaaactata 9721 taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac tcgactttgg 9781 ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt atcaaagatg 9841 ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat gataaagcta 9901 tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta cctggcgcaa 9961 ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat aatcctagtg 10021 aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt cctcaaaaag 10081 gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat ttttataaac 10141 agtctataag taagcatccg gagtggaaag attttggatt tagattttct gatactcaaa 10201 gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac aaactaactt 10261 ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa ttgtacctat 10321 tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat ctacatactt 10381 tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat aagctaaatg 10441 gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact cacccagcta 10501 aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt tttgaatatg 10561 atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt cctattacaa 10621 tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta ttgctaaaag 10681 aaaaagcaaa tgatgttcat atattaagta tagatagagg tgaaagacat ttagcttact 10741 atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac atcattggta 10801 atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa gatagggatt 10861 cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag ggctatctat 10921 ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt gtggtttttg 10981 aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag gtctatcaaa 11041 agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat aatgagtttg 11101 ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag acttttaaaa 11161 agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact tcaaaaattt 11221 gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc agcaaatctc 11281 aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc tattttgagt 11341 ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg actatagcta 11401 gctttgggag tagattgatt aactttagaa attcagataa aaatcataat tgggatactc 11461 gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct atcgaatatg 11521 ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag ttttttgcta 11581 agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt 11641 tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat tcgcgacagg 11701 cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt gggctaaaag 11761 gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc aatttggtta 11821 tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaagaa ttcttgacag 11881 ctagctcagt cctaggtata atgctagcgc tgatttaggc aaaaacgggt ctaagaactt 11941 taaataattt ctactgttgt agatcttcgg tcgcttgatc tttcgtctaa gaactttaaa 12001 taatttctac tgttgtagat gctactattc ctgtgccttc agataattca gtctaagaac 12061 tttaaataat ttctactgtt gtagatgtct agagcctttt gtattagtag ccggtctaag 12121 aactttaaat aatttctact gttgtagatt agcgatttat gaaggtcatt tttttgtcta 12181 gctttaatgc ggtagttggt accgagtggg taaccagcac cgacaaccgc atctacgtgg 12241 gttggttcgg cgtgctgatg atccccactc tgctgaccgc caccatctgc ttcatcgttg 12301 cgttcattgc agcccctccc gtcgacatcg acggcatccg tgagcccgtt gccggctctc 12361 tcatgtatgg caacaacatc atttccggcg ctgttgttcc ttccagcaac gccatcggcc 12421 tgcatttcta tccgatttgg gaagccgcta gcctcgacga gtggctgtac aacggtggtc 12481 cttaccaatt agtggtcttc cacttcttgc tgggtatcag ctgctacatg ggtcgtcaat 12541 gggagctgtc gtaccgcctc ggtatgcgcc cttggatctg tgttgcatac agtgctccac 12601 tctcggctgc ttttgcagtg tttctgatct acccgatcgg ccaaggttcg ttctcggacg 12661 gcatgcccct gggtatcagc ggcaccttca acttcatgtt cgtgttccaa gcagagcaca 12721 acattttgat gcaccccttc cacatgctgg gtgtggctgg tgtgttcggt ggttcgctgt 12781 tctcggcaat gcacggttcg ttggtgacca gctcgctggt gcgtgagacg accgagaccg 12841 agagccaaaa ctacggctac aaatttggtc aagaggaaga gacctacaac atcgtggcag 12901 cccacggata cttcggtcgc ttgatctttc aatacgcagc gttcaacaac agccgttcgc 12961 tgcacttctt cctgggtgca tggccggtcg tgggcatctg gtttacctcc atgggcatca 13021 gcaccatggc gttcaacctg aatggtttca acttcaacca gtcggttttg gatagccaag 13081 gcaaagtgat caacacttgg gcagatgtgt tgaaccgtgc caacttgggc atggaagtga 13141 tgcacgagcg taatgctcac aacttcccgc tcgacttggc agcaggcgaa gcgaccccgg 13201 tcgctttgac tgcgccttca attcacggtt aattgccgga attacagcag tctgaataat 13261 caaaaagcgc ctcgaatggg gcgctttttg atgcttggat attgattgta gacaaccttt 13321 ggacttgcga tcgctaggga ttagagatcg tccagcagat cctcttcgtt gctgttttca 13381 gtcggggttt gaatgccgaa aacggcagaa cccagcaagc tgacatccag ctccattcgt 13441 tgctccattt gtcggagaaa atagcctgtc atcatagctg aggcgagtaa gccagctaaa 13501 ttttcccgac tggttgcgat ttgaacattg aagccttcac tgggcaacat tcccagcaga 13561 ccttgaacat tttgttgaat gatttcttgg atatctgaag tcgccgactt tgctacccgc 13621 gccatcactt ctggtgactg atgctggagg tactgcaaca acgcattgcc cttaagggtt 13681 tccgtgtcgc tgtggaaaaa gtcagaacta aagaccatta cggcggttca gaaacgtacc 13741 caaactctat cgcatcacca ttaccacgtg ggtttggatc caagttcggt ttccagaact 13801 tgctaggttc gttagtcgac tctagaggat ctgtaatccg ggcagcgcaa cggaa //