LOCUS       Exported                8276 bp DNA     circular SYN 11-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1242..2051
                     /locus_tag="Kan"
                     /label=Kan
     misc_feature    2872..4709
                     /locus_tag="CAGpro"
                     /label=CAGpro
     misc_feature    4802..5515
                     /locus_tag="human CD63"
                     /label=human CD63
     misc_feature    5516..5563
                     /locus_tag="linker"
                     /label=linker
     misc_feature    5564..6262
                     /locus_tag="mScarlet"
                     /label=mScarlet
     misc_feature    6326..6550
                     /locus_tag="bGH polyA signal"
                     /label=bGH polyA signal
     misc_feature    7038..7367
                     /locus_tag="SV40pro"
                     /label=SV40pro
     misc_feature    7376..7975
                     /locus_tag="PuroR"
                     /label=PuroR
ORIGIN
        1 ctagcgatat caattaaccc tcactaaagg gagaccaagt taaacaattt aaaggcaatg
       61 ctaccaaata ctaattgagt gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa
      121 ataaaagctg aaatgaatca ttctctctac tattattctg atatttcaca ttcttaaaat
      181 aaagtggtga tcctaactga cctaagacag ggaattttta ctaggattaa atgtcaggaa
      241 ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac
      301 tgtataggga tctggtacca tttaaatctg ttccgcttcc tcgctcactg actcgctgcg
      361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
      961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1201 gatccttttt gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
     1261 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     1321 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     1381 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     1441 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     1501 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     1561 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     1621 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     1681 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     1741 gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     1801 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     1861 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     1921 ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     1981 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga
     2041 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     2101 gatgcacctg gatccagatc cctatacagt tgaagtcgga agtttacata cacttaagtt
     2161 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
     2221 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
     2281 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
     2341 ttacatacac taagttgact gtgcctttaa acagcttgga agctgcgcac taggcaagtt
     2401 aactaactcc tctgaatgtc agtatttcca tctgtaagat gaacacagtg gggctccaat
     2461 tccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2521 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2581 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2641 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggcca gctagtcgac
     2701 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     2761 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     2821 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     2881 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     2941 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     3001 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3061 agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc
     3121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     3301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     3361 attattttgt gcagcgatgg gggcgggggg ggggggcgcg cgccaggcgg ggcggggcgg
     3421 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     3481 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     3541 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
     3601 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
     3661 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
     3721 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     3781 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     3841 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     3901 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     3961 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     4021 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     4081 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     4141 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     4201 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     4261 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     4321 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     4381 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     4441 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     4501 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     4561 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     4621 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     4681 gccaatgccc tggctcacaa ataccactga gatctgcatg agctagcgtt taaacttaag
     4741 cttggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga tagccgccac
     4801 catggcggtg gaaggaggaa tgaaatgtgt gaagttcttg ctctacgtcc tcctgctggc
     4861 cttttgcgcc tgtgcagtgg gactgattgc cgtgggtgtc ggggcacagc ttgtcctgag
     4921 tcagaccata atccaggggg ctacccctgg ctctctgttg ccagtggtca tcatcgcagt
     4981 gggtgtcttc ctcttcctgg tggcttttgt gggctgctgc ggggcctgca aggagaacta
     5041 ttgtcttatg atcacgtttg ccatctttct gtctcttatc atgttggtgg aggtggccgc
     5101 agccattgct ggctatgtgt ttagagataa ggtgatgtca gagtttaata acaacttccg
     5161 gcagcagatg gagaattacc cgaaaaataa ccacactgct tcgatcctgg acaggatgca
     5221 ggcagatttt aagtgctgtg gggctgctaa ctacacagat tgggagaaaa tcccttccat
     5281 gtcgaagaac cgagtccccg actcctgctg cattaatgtt actgtgggct gtgggattaa
     5341 tttcaacgag aaggcgatcc ataaggaggg ctgtgtggag aagattgggg gctggctgag
     5401 gaaaaatgtg ctggtggtag ctgcagcagc ccttggaatt gcttttgtcg aggttttggg
     5461 aattgtcttt gcctgctgcc tcgtgaagag tatcagaagt ggctacgagg tgatgggaga
     5521 tctaggaggt ggaggtagcg gtggaggtgg aagccaggat ccgatggtga gcaagggcga
     5581 ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca
     5641 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     5701 gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt
     5761 catgtacggc tccagggcct tcaccaagca ccccgccgac atccccgact actataagca
     5821 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt
     5881 gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg
     5941 cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc
     6001 gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct
     6061 gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa
     6121 gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa
     6181 cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg
     6241 catggacgag ctgtacaagt aaatccagca cagtggcggc cgctcgagtc tagagggccc
     6301 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6361 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6421 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6481 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6541 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6601 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6661 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6721 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6781 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6841 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6901 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6961 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7021 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7081 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7141 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7201 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7261 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7321 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccatgac
     7381 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     7441 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     7501 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7561 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7621 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7681 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7741 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7801 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7861 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7921 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgattcga
     7981 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8041 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8101 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8221 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcga
//
