LOCUS       Exported                4036 bp ds-DNA     circular SYN 17-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Dina
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 17, 2022 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             14..691
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     polyA_signal    846..901
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1260..1276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1284..1300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1308..1338)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1353..1374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1433..1628
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1479..1614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1634..1768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2007..2595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2766..3626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3627..3731)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3768..3823
                     /label=RARE element
     promoter        3840..4036
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3887..4022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aagcttgcta gccatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt
       61 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      121 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
      181 cgcctgggac atcctgtccc ctcagttcca gtacggctcc aaggcctacg tgaagcaccc
      241 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      301 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      361 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      421 gcagaagaag accatgggct gggaggcctc caccgagcgg atgtaccccg aggacggcgc
      481 cctgaagggc gagatcaaga tgaggctgaa gctgaaggac ggcggccact acgacgccga
      541 ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga
      601 catcaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
      661 cgccgagggc cgccactcca ccggcgccta agaatgcggc cgcactcctc aggtgcaggc
      721 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
      781 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
      841 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
      901 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
      961 gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc
     1021 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     1081 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     1141 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     1201 ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc gtaatcatgg
     1261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     1441 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1561 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1621 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1681 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1741 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     1801 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1861 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1921 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1981 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2041 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2101 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2161 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2221 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2281 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2341 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2401 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2461 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2521 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2581 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2641 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2701 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2761 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2821 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2881 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2941 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3001 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3061 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3121 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3181 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3241 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3301 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3361 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3421 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3481 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3541 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3601 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3661 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3721 ggggttccgc gcacatttcc ccgaaaagtg ccacctgtcg acaattgcag ttgggtcatt
     3781 tgaaggttag cagcccgggt agggttcacc gaaagttcac tcgctcgaga tctgcgatct
     3841 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3901 tccgcccagt tccgcccatt ctccgcccca tcgctgacta atttttttta tttatgcaga
     3961 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4021 cctaggcttt tgcaaa
//
