LOCUS       Exported                9866 bp ds-DNA     circular SYN 06-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      complement(13..39)
                     /label=KanR Terminator
                     /note="color: #93ff35; direction: LEFT"
     CDS             complement(40..855)
                     /label=KanR
                     /note="color: #008000; direction: LEFT"
     primer_bind     53..73
                     /label=MS17_KanF
                     /note="color: black; sequence: tcgagcatcaaatgaaactgc"
     promoter        complement(856..1003)
                     /label=KanR Promoter
                     /note="color: #93ff35; direction: LEFT"
     rep_origin      complement(1004..1767)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     primer_bind     1139..1158
                     /label=EC22_ColE1
                     /note="color: black; sequence: cagcggtggtttgtttgccg"
     primer_bind     1719..1740
                     /label=MM19
                     /note="color: black; sequence: tgctcacatgttctttcctgcg"
     primer_bind     1721..1740
                     /label=Je06_lvl0seq_F
                     /note="color: black; sequence: ctcacatgttctttcctgcg"
     protein_bind    1776..1779
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    1780..2279
                     /label=HO 5' Homology
                     /note="color: #6685ca"
     primer_bind     2123..2142
                     /label=IL53_Full_ampli_fw
                     /note="color: black; sequence: GCTCTACGAGCCTGTTGGAG"
     primer_bind     2230..2254
                     /label=IL80_pYAP_ampli_fw
                     /note="color: black; sequence: GCACATCGATTATTTGATACCCCTT"
     primer_bind     2230..2252
                     /label=IL48_HO5_full_fw
                     /note="color: black; sequence: GCACATCGATTATTTGATACCCC"
     misc_feature    complement(2280..2299)
                     /label=HO 5' barcode
                     /note="color: #8c3031; direction: LEFT"
     primer_bind     complement(2280..2299)
                     /label=MM_HO (2)
                     /note="color: black; sequence: ACAGCGATGGAACTTACGGC"
     protein_bind    2300..2303
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    2319..2343
                     /label=ConS scar
                     /note="color: #fffd6c"
     protein_bind    2319..2322
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     2340..2361
                     /label=MM29
                     /note="color: black; sequence: gacgaacgCCTTGCAATTAGCT"
     primer_bind     2344..2374
                     /label=Haa1-Promoter Primer Type2_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAaacgCCTTGCAATTAGCTTCTATTACACCTG"
     misc_feature    2344..2347
                     /label=Adaptor for Type2 entry vector
                     /note="color: #ff00ff"
     misc_feature    2348..3325
                     /label=HAA1 Native Promoter
                     /note="color: #b1ff67"
     primer_bind     2602..2619
                     /label=PrimerMM0_1-2
                     /note="color: black; sequence: CAACGTTGCCATCGTTGG"
     primer_bind     2797..2815
                     /label=MM30
                     /note="color: black; sequence: AATTGGTGGTGCCGGTTGT"
     primer_bind     3000..3022
                     /label=PrimerMM0_1-3
                     /note="color: black; sequence: GGTCTTAGTAATACAGAGTCGTG"
     primer_bind     3012..3031
                     /label=EC112_pHAA1_check
                     /note="color: black; sequence: ACAGAGTCGTGCATTTCCAC"
     primer_bind     3136..3155
                     /label=LT44_sgRNAcheck_HAA1
                     /note="color: black; sequence: GAGCCAAGACAAAACGTGGC"
     primer_bind     complement(3278..3329)
                     /label=Haa1 Promoter Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataTATTTTTTAGGTTTTTTTTTTCTATACTTTTGTTTCC
                     TTTTTCTTGTC"
     primer_bind     3325..3359
                     /label=Haa1 Primer Type3a_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAtatgATGGTCTTGATAAATGGCATAAAGTATGCC"
     misc_feature    3326..3329
                     /label=Adaptor for Type 3a Entry Vector
                     /note="color: #ff00ff"
     misc_feature    3330..5411
                     /label=HAA1
                     /note="color: #b7e6d7"
     primer_bind     3330..3359
                     /label=Haa1-CFP type 3b FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAttctATGGTCTTGATAAATGGCATAAAGTATGCC"
     primer_bind     3363..3382
                     /label=EC81_HAA1_F2
                     /note="color: black; sequence: GAGAGGTGCATAAGAGGCCA"
     primer_bind     complement(3441..3461)
                     /label=EC113_HAA1_check
                     /note="color: black; sequence: GTCGCATGTAGTGGAAGGTCT"
     primer_bind     complement(3441..3461)
                     /label=EC82_HAA1_R2
                     /note="color: black; sequence: GTCGCATGTAGTGGAAGGTCT"
     primer_bind     3606..3626
                     /label=primerMM0_2-2
                     /note="color: black; sequence: TGCGGGACTGATGAGGTTTGC"
     primer_bind     3763..3782
                     /label=EC79_HAA1_F
                     /note="color: black; sequence: GCCACGGAGCACTATCAGAT"
     primer_bind     complement(3818..3837)
                     /label=EC80_HAA1_R
                     /note="color: black; sequence: TTTTACCAACACCCGGCTCA"
     primer_bind     4044..4063
                     /label=MM32
                     /note="color: black; sequence: TCGGTAAGCTTGCCAGCGAA"
     primer_bind     complement(4056..4075)
                     /label=MS37
                     /note="color: black; sequence: CGTGAATCAGCTTTCGCTGG"
     primer_bind     4110..4129
                     /label=primerMM0_2-3
                     /note="color: black; sequence: CTTGACCTATTAGGCCATTC"
     primer_bind     4289..4308
                     /label=MM2s
                     /note="color: black; sequence: TACAACCGCAACCGCTGCAA"
     primer_bind     4605..4623
                     /label=primerMM0_2-4
                     /note="color: black; sequence: AATCCTCAACTTCGTCATC"
     primer_bind     4653..4674
                     /label=MM33
                     /note="color: black; sequence: CATCATCCGGCAAACGAGTATT"
     primer_bind     4920..4939
                     /label=MM3s
                     /note="color: black; sequence: GCCGTAGCAAGCAATCTCGA"
     primer_bind     complement(5075..5096)
                     /label=MM21_Rw
                     /note="color: black; sequence: CTTGTCTTGGCGACTTCCAGTT"
     primer_bind     5136..5154
                     /label=primerMM0_2-5
                     /note="color: black; sequence: AACATACACGATCTGTCAC"
     primer_bind     complement(5382..5417)
                     /label=Haa1 Primer Type3a_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAagaaCCTAACGAAGACATGAAATTATCCAAATCCGC"
     primer_bind     complement(5382..5414)
                     /label=Haa1/Haa1prom Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataccTAACGAAGACATGAAATTATCCAAATCCGC"
     misc_feature    5412..5417
                     /label=Gly-Met bridge
                     /note="color: #a6acb3"
     misc_feature    5412..5413
                     /label=Stop Codon substitute
                     /note="color: #3366ff"
     protein_bind    5414..5417
                     /label=BsaI
                     /note="color: #00ff2c"
     CDS             5418..6131
                     /label=mTurquoise2
                     /note="color: #40e0d0; direction: RIGHT"
     primer_bind     5438..5459
                     /label=EC103_VENUS_F
                     /note="color: black; sequence: ATTCACTGGTGTTGTCCCAATT"
     primer_bind     complement(5502..5521)
                     /label=EC104_VENUS_R
                     /note="color: black; sequence: CCTTCACCTTCACCGGAGAC"
     primer_bind     complement(5937..5961)
                     /label=EC65_Venus_check
                     /note="color: black; sequence: aatggtcagctaattgaacaccacc"
     primer_bind     5937..5958
                     /label=EC105_VENUS_F2
                     /note="color: black; sequence: GGTGGTGTTCAATTAGCTGACC"
     primer_bind     complement(6106..6138)
                     /label=Haa1-CFP type 3b RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAggatcctttgtacaattcatccatacccaagg"
     protein_bind    6134..6137
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      6147..6371
                     /label=ScENO1 Terminator
                     /note="color: #ff8eff"
     primer_bind     complement(6160..6179)
                     /label=Je02tENO1SeqR
                     /note="color: black; sequence: tgttttttggactagaaggc"
     primer_bind     6318..6338
                     /label=MM23
                     /note="color: black; sequence: TATTCTGCTTGTGGTGACGCG"
     primer_bind     complement(6320..6339)
                     /label=EC195_Eno1_TT
                     /note="color: black; sequence: ACGCGTCACCACAAGCAGAA"
     primer_bind     6368..6389
                     /label=MM38
                     /note="color: black; sequence: gtatgctgccaaaccagatgtc"
     protein_bind    6372..6375
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    6376..6400
                     /label=Con1 scar
                     /note="color: #fffd6c"
     protein_bind    6376..6379
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     6401..6435
                     /label=YGP1 Primer Type2_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAaacgGAGCAATAGGAAAATTCAGTAGTCGATTTCC"
     misc_feature    6401..6404
                     /label=Adaptor for Type2 entry vector
                     /note="color: #ff00ff"
     misc_feature    6405..7285
                     /label=YGP1-HREs Promoter
                     /label=YGP1 Native Promoter
                     /note="color: #b4abac"
     misc_feature    6438..6446
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6459..6467
                     /label=HRE2
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    complement(6468..6472)
                     /label=RGM1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    6532..6536
                     /label=RGM1 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    6549..6558
                     /label=MIG1 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    6557..6565
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    complement(6569..6578)
                     /label=MIG1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(6585..6591)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    6590..6598
                     /label=My change - 2
                     /note="color: #ff00ff; direction: RIGHT"
     misc_feature    complement(6597..6604)
                     /label=GCN4 BS
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     6631..6650
                     /label=MM34
                     /note="color: black; sequence: ACATCGGCCATTCGTTGGTT"
     misc_feature    complement(6654..6660)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     complement(6693..6706)
                     /label=MM22_Rw
                     /note="color: black; sequence: GATCTTCCGCGGATGACGAG"
     misc_feature    6707..6715
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6708..6715
                     /label=HRE2
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    6715..6719
                     /label=CHA4 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(6757..6763)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(6785..6791)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(6824..6831)
                     /label=HRE1
                     /note="color: #99cc00; direction: LEFT"
     misc_feature    6835..6844
                     /label=GAT3 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    6848..6856
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    6852..6856
                     /label=RPH1 BS
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     6895..6917
                     /label=MM8s
                     /note="color: black; sequence: TTCCTAATGGAGAGGCTCAGGAG"
     misc_feature    6921..6929
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    6922..6929
                     /label=HRE1
                     /note="color: #99cc00; direction: RIGHT"
     misc_feature    6972..6980
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    complement(7037..7045)
                     /label=PHO2 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    7058..7066
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7075..7083
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    7147..7155
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7199..7207
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7237..7244
                     /label=HCM1 BS
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(7248..7289)
                     /label=YGP1 Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataTTTCTATTACTGTATTACTTAACTGACGATTAATCCT
                     G"
     primer_bind     7285..7304
                     /label=mCherry Type 3 FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAtatggtgagcaagggcgag"
     primer_bind     7286..7306
                     /label=MM35
                     /note="color: black; sequence: tatggtgagcaagggcgagga"
     misc_feature    7286..7289
                     /label=Adaptor for Type 3
                     /note="color: #ff00ff"
     CDS             7287..7997
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7430..7450)
                     /label=LT82_mCherry_check_R
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGGCG"
     primer_bind     complement(7432..7450)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGG"
     primer_bind     7700..7719
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     primer_bind     7710..7731
                     /label=LT83_mCherry_check_F
                     /note="color: black; sequence: CAGAAGAAGACTATGGGCTGGG"
     primer_bind     7913..7934
                     /label=MM36
                     /note="color: black; sequence: caacgaggactacaccatcgtg"
     primer_bind     complement(7974..8002)
                     /label=mCherry Type 3 Rw
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAggatttacttgtacagctcgtccatgcc"
     protein_bind    7998..8001
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      8011..8235
                     /label=ScSSA1 Terminator
                     /note="color: #ff8eff"
     protein_bind    8236..8239
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    8240..8264
                     /label=ConE scar
                     /note="color: #fffd6c"
     protein_bind    8240..8243
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     complement(8274..8295)
                     /label=MM24
                     /note="color: black; sequence: TCAAGGAAACCGTGTACTGCAG"
     protein_bind    8280..8283
                     /label=BsaI
                     /note="color: #00ff2c"
     promoter        8284..8460
                     /label=ScURA3 Promoter
                     /note="color: #ffb334"
     CDS             8461..9264
                     /label=URA3
                     /note="color: #ff9127; direction: RIGHT"
     primer_bind     8565..8587
                     /label=MM37
                     /note="color: black; sequence: attggatgttcgtaccaccaagg"
     terminator      9265..9342
                     /label=ScURA3 Terminator
                     /note="color: #ffcb72"
     protein_bind    9343..9346
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    9347..9366
                     /label=HO 3' barcode
                     /note="color: #0e7002"
     primer_bind     9347..9366
                     /label=MM_HO (3)
                     /note="color: black; sequence: TATCGTGTTGCATCTGCGGC"
     misc_feature    9367..9866
                     /label=HO 3' Homology
                     /note="color: #6685ca"
     primer_bind     complement(9399..9416)
                     /label=IL49_HO3_full_rev
                     /note="color: black; sequence: GCTCAGGGCACTGTACTG"
     primer_bind     complement(9451..9470)
                     /label=IL54_Full_ampli_rev
                     /note="color: black; sequence: GCTGCGTGCTGGAATTATGT"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgccaatt ctaattgtat cgagatcact
     1801 tttcgtgatc cgctaatcag cgacggtcac attaggtttg ccaagtcagg gtatgaacca
     1861 tacgatcagt tttcgtgaac ctggtacgta tattgtggcg tttgtgtata ttttcattct
     1921 ttgacaacaa tcaataccaa cctcaaatag gaaaagtaat aagtttggcg ttacacccca
     1981 aaagacgcca aacggatcga acttactcaa tagcaattag cgagacaaaa cctacgttaa
     2041 gacctgtaac cgatttatca aagcactctg cggttctttc ttgggaatat tacctggaca
     2101 ttttgtgccc tcaagaaacg aggctctacg agcctgttgg agcccctcag acattagccg
     2161 ccacgaatca aactttttac gcgattcggc ccaaatcagt ttctcacaga tcattcgtag
     2221 agtgaaaaag cacatcgatt atttgatacc cctttgggtt aattactgtt gaggtctttg
     2281 ccgtaagttc catcgctgtc cctgaattcg catctagact gaactggccg ataattgcag
     2341 acgaacgcct tgcaattagc ttctattaca cctgatgact gtttttcaat ctttatgtga
     2401 tctttttaca cccaatgtga taaagtacta gtaacatgaa tactactaaa cagatgatac
     2461 tttagagttt tcattccaac actcaattgt aaaaataaga caatattatg attttttggc
     2521 tgtataacca catctattta gtagcagggt gacatgttac tcccacacaa tacttcgcgc
     2581 aagtggttta gtggtaaaat ccaacgttgc catcgttggg cccccggttc gattccgggc
     2641 ttgcgcaatt tttccccctc gccctgaagg gcgtaacgtt aacgcttccg gtaatttgcg
     2701 aataattttt cttcccattt cccctttctt ttccctttaa catgtggagc atagcaggct
     2761 ctttactttt ttacgcattc agacagtcgg gaggtcaatt ggtggtgccg gttgtgggaa
     2821 agaggtaatt ttatttcctt tattggacat gatttcaata gtcacattgg ggcaggaaaa
     2881 agaaaaggga gaaaaaaatt cagtaaagaa aaaaaataag aaaaagagaa aagaaacaag
     2941 agagggctac agatttctaa atatcctttt gctgcacatt ttttatttac ctgctaattg
     3001 gtcttagtaa tacagagtcg tgcatttcca ctttataaga aaaaaaaaag ggataacagc
     3061 accagcactt gattgaaaac tagaaagttc acgttttata agtcctttcc gttctcttta
     3121 tcagttataa taaaagagcc aagacaaaac gtggcaggga agcttttcca aggaaaagag
     3181 ggttgtaata ttaaacaaaa accgtaatca agcaaaataa gaccccgtgg ttttttatca
     3241 taggaaaaga gttctctgtt gtcactctac catatttgac aagaaaaagg aaacaaaagt
     3301 atagaaaaaa aaaacctaaa aaatatatga tggtcttgat aaatggcata aagtatgcct
     3361 gtgagaggtg cataagaggc catagagtaa caacatgcaa tcatacagat caaccgctta
     3421 tgatgatcaa acccaaaggt agaccttcca ctacatgcga ctattgtaaa caacttcgaa
     3481 aaaacaagaa tgcaaatcct gaaggtgttt gcacgtgtgg ccggctagag aagaaaaaac
     3541 tggcacagaa agccaaagaa gaagcaagag ctaaagccaa agaaaaacaa agaaaacagt
     3601 gtacctgcgg gactgatgag gtttgcaaat atcatgctca aaagagacat ctaagaaagt
     3661 ccccttcaag ttctcaaaag aaaggaagat ccatttctcg ttctcaacca atgtttgaaa
     3721 gggtattgtc ttctacttca cttgacagca atatgttatc cggccacgga gcactatcag
     3781 atacctctag catactgacg agcacatttt tagacagtga gccgggtgtt ggtaaaattt
     3841 caaaagatta ccatcatgtc ccttcattgg cctccatttc atccttacaa tcctcgcaat
     3901 cgttagatca aaatttcagt ataccacaaa gcccgccgtt atcttcaatg tcatttaatt
     3961 ttctcacggg aaatatcaat gaaaccaacc aaaatcacag taatcatcag cattcaaaat
     4021 caggcaataa ctggcaagat agttcggtaa gcttgccagc gaaagctgat tcacgtctta
     4081 acatgatgga taaaaacaac tctgtgggtc ttgacctatt aggccattca aaacgaatat
     4141 cgccgatatc aaactctcgt gtgggcgaag ttagcgttcc gctagaagaa tatattcctt
     4201 ctgacattga tggggttgga agagttactg ataaaagctc tttggtctac gattggccat
     4261 ttgatgaaag tattgagaga aatttcagta caaccgcaac cgctgcaact ggtgaaagta
     4321 agttcgacat taacgacaac tgtaatagaa ttaatagcaa aagttatagt aagactaata
     4381 gtatgaatgg aaacggtatg aacaatagca ataataataa tatcaacagt aatggcaacg
     4441 acaagaacaa taacaactct tctagacaag aacatcaagg aaatggacta tttgacatgt
     4501 ttacagattc atcgtcgatt tcaacgcttt cccgtgcaaa cttattattg caagaaaaaa
     4561 ttggttcgca agaaaactct gtcaaacaag aaaactattc gaaaaatcct caacttcgtc
     4621 atcaattaac ttccagaagt agatcattta ttcatcatcc ggcaaacgag tatttgaaga
     4681 atacttttgg aaattcacat agtaatgaca tcggaaaggg agttgaagtg ctatctttga
     4741 caccgagttt tatggatatt cccgaaaaag aaagagaaac ggaaagatcg ccatcatcca
     4801 attacattac tgacagacct ttcactcgaa aacctagatc ttctagcatt gacgtaaacc
     4861 ataggtatcc acctatggca ccaacaaccg tagcgacatc tcccggtgca ttgaacaatg
     4921 ccgtagcaag caatctcgac gatcaactga gtttaacatc actaaactct cagccatcat
     4981 cgatagcaaa tatgatgatg gacccttcaa acctagctga gcaaagttct attcattcag
     5041 ttcctcagtc aataaactct ccgagaatgc ctaaaactgg aagtcgccaa gacaagaaca
     5101 ttcacactaa gaaggaagaa agaaatccgc taaataacat acacgatctg tcacaattgg
     5161 aaaatgtacc agacgagatg aaccaaatgt tctccccacc attaaaaagt atgaatagac
     5221 cggatgccat aagggaaaat tcatctagta gtaatttcat aatccaagga aatagcatga
     5281 tctctacgcc ttccggaagg aatgaccttc cagatacctc tccaatgagt agtattcaaa
     5341 cagcgtcacc accaagtcaa ttactgaccg atcaaggatt tgcggatttg gataatttca
     5401 tgtcttcgtt aggttctgtt tctaaaggtg aagaattatt cactggtgtt gtcccaattt
     5461 tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag
     5521 gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag
     5581 ttccatggcc aaccttagtc actactttat cttggggtgt tcaatgtttt gcaagatacc
     5641 cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag
     5701 aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg
     5761 aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta
     5821 acattttagg tcacaaattg gaatacaatt atttctctga caatgtttac atcactgctg
     5881 acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt gaagatggtg
     5941 gtgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt
     6001 taccagacaa ccattactta tccactcaat ctaagttatc caaagatcca aacgaaaaga
     6061 gggaccacat ggtcttgtta gaatttgtta ctgctgctgg tattaccttg ggtatggatg
     6121 aattgtacaa aggatcctaa ctcgagagct tttgattaag ccttctagtc caaaaaacac
     6181 gtttttttgt catttatttc attttcttag aatagtttag tttattcatt ttatagtcac
     6241 gaatgtttta tgattctata tagggttgca aacaagcatt tttcatttta tgttaaaaca
     6301 atttcaggtt taccttttat tctgcttgtg gtgacgcgtg tatccgcccg ctcttttggt
     6361 cacccatgta tgctgccaaa ccagatgtca acacagctac aacggagcaa taggaaaatt
     6421 cagtagtcga tttccatggc gaggggctat tgggtaatgt ggcggcgagg gctaaacatt
     6481 cggtatcatt gattcaatgt tctcacaatg ttcaaattct cggatcgggt atccctaatt
     6541 gggatctctt tccagaggcg aggggcttga tgtgggattg aagacacatg agtttttttc
     6601 tcaccgtcga aacttttgct tacccgtaaa acatcggcca ttcgttggtt tattatgtga
     6661 cttttttgtg actttttttt ttgcgaagtt ttctcgtcat ccgcggggcg aggggcgggc
     6721 gaaaaagtct ctcactttta cccaatcggg gcatgttatg tggacatgtt atgtgtccaa
     6781 gagacacatg agcccaaaaa gggtacactg tctaaatagt ttacccttta cgaatcgaaa
     6841 cttctcgggc gaggggccga aaattagcgc agaagttgac gaaagtttat acttttccta
     6901 atggagaggc tcaggagctg ggcgagggga tattcgtact ctattgcatc ttcaaactcc
     6961 gaagaatctc aggcgagggg gaagccggct tctcgatgct acagttcaac atcgatactt
     7021 tgaagaaaga aagcgcctat tatatctctt ttaccctggc gaggggaatt aggaggcgag
     7081 gggaaaaaag tacctcacta aaaaaaccat catctctgaa atataaaaag cttgatagag
     7141 ggtgacggcg aggggaactt ctgctgtgtt ctcttgggtt attgctctta ttgaatatgg
     7201 cgaggggttc tttcttgctt gtaaaaaatc agctcaaaaa acatctacag gattaatcgt
     7261 cagttaagta atacagtaat agaaatatgg tgagcaaggg cgaggaggat aacatggcca
     7321 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtggac ggccacgagt
     7381 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     7441 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     7501 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     7561 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7621 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     7681 ccaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg gaggcctcct
     7741 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     7801 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     7861 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     7921 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     7981 acgagctgta caagtaaatc ctaactcgag gccaattggt gcggcaattg ataataacga
     8041 aaatgtcttt taatgatctg ggtataatga ggaattttcc gaacgttttt actttatata
     8101 tatatataca tgtaacatat attctatacg ctatagagaa aggaaatttt tcaattaaaa
     8161 aaaaaataga gaaagagttt cacttcttga ttatcgctaa cactaatggt tgaagtactg
     8221 ctactttaat tttatgctga gcagttacag agatgttacg aaccactagt gcactgcagt
     8281 acacggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
     8341 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
     8401 tgcccagtat tcttaaccca actgcacaga acaaaaacgt gcaggaaacg aagataaatc
     8461 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
     8521 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
     8581 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
     8641 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
     8701 tccgccaagt acaatttttt actcttcgag gacagaaaat ttgctgacat tggtaataca
     8761 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
     8821 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
     8881 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
     8941 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
     9001 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
     9061 acacccggtg tgggtttaga tgacaaggga gatgcattgg gtcaacagta tagaaccgtg
     9121 gatgatgtgg tttctacagg atctgacatt attattgttg gaagaggact atttgcaaag
     9181 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
     9241 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
     9301 tcacaaatta gagcttcaat ttaattatat cagttattac ccgagttatc gtgttgcatc
     9361 tgcggcttta aattgatgta tctcatcgca ggcacgggca gtacagtgcc ctgagcgtag
     9421 ggaaaaatga aaaaaaggat gtaactttta acataattcc agcacgcagc gattgggtat
     9481 aatgaagatt gttaagttca acaacatgtg aaattcttag ctccgcaact tgcttgaatt
     9541 atgagctcta agattcaaga agtaaatagg tcatataatg gaatgatagt agtacttgat
     9601 aaaggaacca tgtgatctta cgttgatatg aaaataattc tctcacagaa gtcagtttgt
     9661 atgactaaca tagagagtaa ttgcttttcg gataaaatcg cacctaacaa agattacctt
     9721 ttgtaaagcc tccagaacag ctatgaatgt tcgttattct acgcactttc tttcatacat
     9781 cttgatccgc cttttttgat gtaggtttta acgcggcaaa acatattaat ttttctgatt
     9841 tggaaaaata aagagttagt tctgcc
//
