LOCUS       Exported                9358 bp ds-DNA     circular SYN 06-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      complement(13..39)
                     /label=KanR Terminator
                     /note="color: #93ff35; direction: LEFT"
     CDS             complement(40..855)
                     /label=KanR
                     /note="color: #008000; direction: LEFT"
     primer_bind     53..73
                     /label=MS17_KanF
                     /note="color: black; sequence: tcgagcatcaaatgaaactgc"
     promoter        complement(856..1003)
                     /label=KanR Promoter
                     /note="color: #93ff35; direction: LEFT"
     rep_origin      complement(1004..1767)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     primer_bind     1139..1158
                     /label=EC22_ColE1
                     /note="color: black; sequence: cagcggtggtttgtttgccg"
     primer_bind     1719..1740
                     /label=MM19
                     /note="color: black; sequence: tgctcacatgttctttcctgcg"
     primer_bind     1721..1740
                     /label=Je06_lvl0seq_F
                     /note="color: black; sequence: ctcacatgttctttcctgcg"
     protein_bind    1776..1779
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    1780..2279
                     /label=HO 5' Homology
                     /note="color: #6685ca"
     primer_bind     2123..2142
                     /label=IL53_Full_ampli_fw
                     /note="color: black; sequence: GCTCTACGAGCCTGTTGGAG"
     primer_bind     2230..2254
                     /label=IL80_pYAP_ampli_fw
                     /note="color: black; sequence: GCACATCGATTATTTGATACCCCTT"
     primer_bind     2230..2252
                     /label=IL48_HO5_full_fw
                     /note="color: black; sequence: GCACATCGATTATTTGATACCCC"
     misc_feature    complement(2280..2299)
                     /label=HO 5' barcode
                     /note="color: #8c3031; direction: LEFT"
     primer_bind     complement(2280..2299)
                     /label=MM_HO (2)
                     /note="color: black; sequence: ACAGCGATGGAACTTACGGC"
     protein_bind    2300..2303
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    2319..2343
                     /label=ConS scar
                     /note="color: #fffd6c"
     protein_bind    2319..2322
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     2340..2361
                     /label=MM29
                     /note="color: black; sequence: gacgaacgCCTTGCAATTAGCT"
     primer_bind     2344..2374
                     /label=Haa1-Promoter Primer Type2_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAaacgCCTTGCAATTAGCTTCTATTACACCTG"
     misc_feature    2344..2347
                     /label=Adaptor for Type2 entry vector
                     /note="color: #ff00ff"
     misc_feature    2348..3325
                     /label=HAA1 Native Promoter
                     /note="color: #b1ff67"
     primer_bind     2602..2619
                     /label=PrimerMM0_1-2
                     /note="color: black; sequence: CAACGTTGCCATCGTTGG"
     primer_bind     2797..2815
                     /label=MM30
                     /note="color: black; sequence: AATTGGTGGTGCCGGTTGT"
     primer_bind     3000..3022
                     /label=PrimerMM0_1-3
                     /note="color: black; sequence: GGTCTTAGTAATACAGAGTCGTG"
     primer_bind     3012..3031
                     /label=EC112_pHAA1_check
                     /note="color: black; sequence: ACAGAGTCGTGCATTTCCAC"
     primer_bind     3136..3155
                     /label=LT44_sgRNAcheck_HAA1
                     /note="color: black; sequence: GAGCCAAGACAAAACGTGGC"
     primer_bind     complement(3278..3329)
                     /label=Haa1 Promoter Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataTATTTTTTAGGTTTTTTTTTTCTATACTTTTGTTTCC
                     TTTTTCTTGTC"
     primer_bind     3325..3359
                     /label=Haa1 Primer Type3a_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAtatgATGGTCTTGATAAATGGCATAAAGTATGCC"
     misc_feature    3326..3329
                     /label=Adaptor for Type 3a Entry Vector
                     /note="color: #ff00ff"
     misc_feature    3330..5411
                     /label=HAA1
                     /note="color: #b7e6d7"
     primer_bind     3330..3359
                     /label=Haa1-CFP type 3b FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAttctATGGTCTTGATAAATGGCATAAAGTATGCC"
     primer_bind     3363..3382
                     /label=EC81_HAA1_F2
                     /note="color: black; sequence: GAGAGGTGCATAAGAGGCCA"
     primer_bind     complement(3441..3461)
                     /label=EC113_HAA1_check
                     /note="color: black; sequence: GTCGCATGTAGTGGAAGGTCT"
     primer_bind     complement(3441..3461)
                     /label=EC82_HAA1_R2
                     /note="color: black; sequence: GTCGCATGTAGTGGAAGGTCT"
     primer_bind     3606..3626
                     /label=primerMM0_2-2
                     /note="color: black; sequence: TGCGGGACTGATGAGGTTTGC"
     primer_bind     3763..3782
                     /label=EC79_HAA1_F
                     /note="color: black; sequence: GCCACGGAGCACTATCAGAT"
     primer_bind     complement(3818..3837)
                     /label=EC80_HAA1_R
                     /note="color: black; sequence: TTTTACCAACACCCGGCTCA"
     primer_bind     4044..4063
                     /label=MM32
                     /note="color: black; sequence: TCGGTAAGCTTGCCAGCGAA"
     primer_bind     complement(4056..4075)
                     /label=MS37
                     /note="color: black; sequence: CGTGAATCAGCTTTCGCTGG"
     primer_bind     4110..4129
                     /label=primerMM0_2-3
                     /note="color: black; sequence: CTTGACCTATTAGGCCATTC"
     primer_bind     4289..4308
                     /label=MM2s
                     /note="color: black; sequence: TACAACCGCAACCGCTGCAA"
     primer_bind     4605..4623
                     /label=primerMM0_2-4
                     /note="color: black; sequence: AATCCTCAACTTCGTCATC"
     primer_bind     4653..4674
                     /label=MM33
                     /note="color: black; sequence: CATCATCCGGCAAACGAGTATT"
     primer_bind     4920..4939
                     /label=MM3s
                     /note="color: black; sequence: GCCGTAGCAAGCAATCTCGA"
     primer_bind     complement(5075..5096)
                     /label=MM21_Rw
                     /note="color: black; sequence: CTTGTCTTGGCGACTTCCAGTT"
     primer_bind     5136..5154
                     /label=primerMM0_2-5
                     /note="color: black; sequence: AACATACACGATCTGTCAC"
     primer_bind     complement(5382..5417)
                     /label=Haa1 Primer Type3a_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAagaaCCTAACGAAGACATGAAATTATCCAAATCCGC"
     primer_bind     complement(5382..5414)
                     /label=Haa1/Haa1prom Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataccTAACGAAGACATGAAATTATCCAAATCCGC"
     misc_feature    5412..5417
                     /label=Gly-Met bridge
                     /note="color: #a6acb3"
     misc_feature    5412..5413
                     /label=Stop Codon substitute
                     /note="color: #3366ff"
     protein_bind    5414..5417
                     /label=BsaI
                     /note="color: #00ff2c"
     CDS             5418..6131
                     /label=mTurquoise2
                     /note="color: #40e0d0; direction: RIGHT"
     primer_bind     5438..5459
                     /label=EC103_VENUS_F
                     /note="color: black; sequence: ATTCACTGGTGTTGTCCCAATT"
     primer_bind     complement(5502..5521)
                     /label=EC104_VENUS_R
                     /note="color: black; sequence: CCTTCACCTTCACCGGAGAC"
     primer_bind     complement(5937..5961)
                     /label=EC65_Venus_check
                     /note="color: black; sequence: aatggtcagctaattgaacaccacc"
     primer_bind     5937..5958
                     /label=EC105_VENUS_F2
                     /note="color: black; sequence: GGTGGTGTTCAATTAGCTGACC"
     primer_bind     complement(6106..6138)
                     /label=Haa1-CFP type 3b RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAggatcctttgtacaattcatccatacccaagg"
     protein_bind    6134..6137
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      6147..6371
                     /label=ScENO1 Terminator
                     /note="color: #ff8eff"
     primer_bind     complement(6160..6179)
                     /label=Je02tENO1SeqR
                     /note="color: black; sequence: tgttttttggactagaaggc"
     primer_bind     6318..6338
                     /label=MM23
                     /note="color: black; sequence: TATTCTGCTTGTGGTGACGCG"
     primer_bind     complement(6320..6339)
                     /label=EC195_Eno1_TT
                     /note="color: black; sequence: ACGCGTCACCACAAGCAGAA"
     primer_bind     6368..6389
                     /label=MM38
                     /note="color: black; sequence: gtatgctgccaaaccagatgtc"
     protein_bind    6372..6375
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    6376..6400
                     /label=Con1 scar
                     /note="color: #fffd6c"
     protein_bind    6376..6379
                     /label=BsmBI
                     /note="color: #00ff15"
     misc_feature    6401..6404
                     /label=Adaptor for Type2 entry vector
                     /note="color: #ff00ff"
     misc_feature    6411..6419
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6435..6443
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6459..6467
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6483..6491
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6498..6506
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6522..6530
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6546..6554
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6570..6578
                     /label=HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    6583..6773
                     /label=ENO1 cp
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     6777..6796
                     /label=mCherry Type 3 FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAtatggtgagcaagggcgag"
     primer_bind     6778..6798
                     /label=MM35
                     /note="color: black; sequence: tatggtgagcaagggcgagga"
     misc_feature    6778..6781
                     /label=Adaptor for Type 3
                     /note="color: #ff00ff"
     CDS             6779..7489
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6922..6942)
                     /label=LT82_mCherry_check_R
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGGCG"
     primer_bind     complement(6924..6942)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGG"
     primer_bind     7192..7211
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     primer_bind     7202..7223
                     /label=LT83_mCherry_check_F
                     /note="color: black; sequence: CAGAAGAAGACTATGGGCTGGG"
     primer_bind     7405..7426
                     /label=MM36
                     /note="color: black; sequence: caacgaggactacaccatcgtg"
     primer_bind     complement(7466..7494)
                     /label=mCherry Type 3 Rw
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAggatttacttgtacagctcgtccatgcc"
     protein_bind    7490..7493
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      7503..7727
                     /label=ScSSA1 Terminator
                     /note="color: #ff8eff"
     protein_bind    7728..7731
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    7732..7756
                     /label=ConE scar
                     /note="color: #fffd6c"
     protein_bind    7732..7735
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     complement(7766..7787)
                     /label=MM24
                     /note="color: black; sequence: TCAAGGAAACCGTGTACTGCAG"
     protein_bind    7772..7775
                     /label=BsaI
                     /note="color: #00ff2c"
     promoter        7776..7952
                     /label=ScURA3 Promoter
                     /note="color: #ffb334"
     CDS             7953..8756
                     /label=URA3
                     /note="color: #ff9127; direction: RIGHT"
     primer_bind     8057..8079
                     /label=MM37
                     /note="color: black; sequence: attggatgttcgtaccaccaagg"
     terminator      8757..8834
                     /label=ScURA3 Terminator
                     /note="color: #ffcb72"
     protein_bind    8835..8838
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    8839..8858
                     /label=HO 3' barcode
                     /note="color: #0e7002"
     primer_bind     8839..8858
                     /label=MM_HO (3)
                     /note="color: black; sequence: TATCGTGTTGCATCTGCGGC"
     misc_feature    8859..9358
                     /label=HO 3' Homology
                     /note="color: #6685ca"
     primer_bind     complement(8891..8908)
                     /label=IL49_HO3_full_rev
                     /note="color: black; sequence: GCTCAGGGCACTGTACTG"
     primer_bind     complement(8943..8962)
                     /label=IL54_Full_ampli_rev
                     /note="color: black; sequence: GCTGCGTGCTGGAATTATGT"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgccaatt ctaattgtat cgagatcact
     1801 tttcgtgatc cgctaatcag cgacggtcac attaggtttg ccaagtcagg gtatgaacca
     1861 tacgatcagt tttcgtgaac ctggtacgta tattgtggcg tttgtgtata ttttcattct
     1921 ttgacaacaa tcaataccaa cctcaaatag gaaaagtaat aagtttggcg ttacacccca
     1981 aaagacgcca aacggatcga acttactcaa tagcaattag cgagacaaaa cctacgttaa
     2041 gacctgtaac cgatttatca aagcactctg cggttctttc ttgggaatat tacctggaca
     2101 ttttgtgccc tcaagaaacg aggctctacg agcctgttgg agcccctcag acattagccg
     2161 ccacgaatca aactttttac gcgattcggc ccaaatcagt ttctcacaga tcattcgtag
     2221 agtgaaaaag cacatcgatt atttgatacc cctttgggtt aattactgtt gaggtctttg
     2281 ccgtaagttc catcgctgtc cctgaattcg catctagact gaactggccg ataattgcag
     2341 acgaacgcct tgcaattagc ttctattaca cctgatgact gtttttcaat ctttatgtga
     2401 tctttttaca cccaatgtga taaagtacta gtaacatgaa tactactaaa cagatgatac
     2461 tttagagttt tcattccaac actcaattgt aaaaataaga caatattatg attttttggc
     2521 tgtataacca catctattta gtagcagggt gacatgttac tcccacacaa tacttcgcgc
     2581 aagtggttta gtggtaaaat ccaacgttgc catcgttggg cccccggttc gattccgggc
     2641 ttgcgcaatt tttccccctc gccctgaagg gcgtaacgtt aacgcttccg gtaatttgcg
     2701 aataattttt cttcccattt cccctttctt ttccctttaa catgtggagc atagcaggct
     2761 ctttactttt ttacgcattc agacagtcgg gaggtcaatt ggtggtgccg gttgtgggaa
     2821 agaggtaatt ttatttcctt tattggacat gatttcaata gtcacattgg ggcaggaaaa
     2881 agaaaaggga gaaaaaaatt cagtaaagaa aaaaaataag aaaaagagaa aagaaacaag
     2941 agagggctac agatttctaa atatcctttt gctgcacatt ttttatttac ctgctaattg
     3001 gtcttagtaa tacagagtcg tgcatttcca ctttataaga aaaaaaaaag ggataacagc
     3061 accagcactt gattgaaaac tagaaagttc acgttttata agtcctttcc gttctcttta
     3121 tcagttataa taaaagagcc aagacaaaac gtggcaggga agcttttcca aggaaaagag
     3181 ggttgtaata ttaaacaaaa accgtaatca agcaaaataa gaccccgtgg ttttttatca
     3241 taggaaaaga gttctctgtt gtcactctac catatttgac aagaaaaagg aaacaaaagt
     3301 atagaaaaaa aaaacctaaa aaatatatga tggtcttgat aaatggcata aagtatgcct
     3361 gtgagaggtg cataagaggc catagagtaa caacatgcaa tcatacagat caaccgctta
     3421 tgatgatcaa acccaaaggt agaccttcca ctacatgcga ctattgtaaa caacttcgaa
     3481 aaaacaagaa tgcaaatcct gaaggtgttt gcacgtgtgg ccggctagag aagaaaaaac
     3541 tggcacagaa agccaaagaa gaagcaagag ctaaagccaa agaaaaacaa agaaaacagt
     3601 gtacctgcgg gactgatgag gtttgcaaat atcatgctca aaagagacat ctaagaaagt
     3661 ccccttcaag ttctcaaaag aaaggaagat ccatttctcg ttctcaacca atgtttgaaa
     3721 gggtattgtc ttctacttca cttgacagca atatgttatc cggccacgga gcactatcag
     3781 atacctctag catactgacg agcacatttt tagacagtga gccgggtgtt ggtaaaattt
     3841 caaaagatta ccatcatgtc ccttcattgg cctccatttc atccttacaa tcctcgcaat
     3901 cgttagatca aaatttcagt ataccacaaa gcccgccgtt atcttcaatg tcatttaatt
     3961 ttctcacggg aaatatcaat gaaaccaacc aaaatcacag taatcatcag cattcaaaat
     4021 caggcaataa ctggcaagat agttcggtaa gcttgccagc gaaagctgat tcacgtctta
     4081 acatgatgga taaaaacaac tctgtgggtc ttgacctatt aggccattca aaacgaatat
     4141 cgccgatatc aaactctcgt gtgggcgaag ttagcgttcc gctagaagaa tatattcctt
     4201 ctgacattga tggggttgga agagttactg ataaaagctc tttggtctac gattggccat
     4261 ttgatgaaag tattgagaga aatttcagta caaccgcaac cgctgcaact ggtgaaagta
     4321 agttcgacat taacgacaac tgtaatagaa ttaatagcaa aagttatagt aagactaata
     4381 gtatgaatgg aaacggtatg aacaatagca ataataataa tatcaacagt aatggcaacg
     4441 acaagaacaa taacaactct tctagacaag aacatcaagg aaatggacta tttgacatgt
     4501 ttacagattc atcgtcgatt tcaacgcttt cccgtgcaaa cttattattg caagaaaaaa
     4561 ttggttcgca agaaaactct gtcaaacaag aaaactattc gaaaaatcct caacttcgtc
     4621 atcaattaac ttccagaagt agatcattta ttcatcatcc ggcaaacgag tatttgaaga
     4681 atacttttgg aaattcacat agtaatgaca tcggaaaggg agttgaagtg ctatctttga
     4741 caccgagttt tatggatatt cccgaaaaag aaagagaaac ggaaagatcg ccatcatcca
     4801 attacattac tgacagacct ttcactcgaa aacctagatc ttctagcatt gacgtaaacc
     4861 ataggtatcc acctatggca ccaacaaccg tagcgacatc tcccggtgca ttgaacaatg
     4921 ccgtagcaag caatctcgac gatcaactga gtttaacatc actaaactct cagccatcat
     4981 cgatagcaaa tatgatgatg gacccttcaa acctagctga gcaaagttct attcattcag
     5041 ttcctcagtc aataaactct ccgagaatgc ctaaaactgg aagtcgccaa gacaagaaca
     5101 ttcacactaa gaaggaagaa agaaatccgc taaataacat acacgatctg tcacaattgg
     5161 aaaatgtacc agacgagatg aaccaaatgt tctccccacc attaaaaagt atgaatagac
     5221 cggatgccat aagggaaaat tcatctagta gtaatttcat aatccaagga aatagcatga
     5281 tctctacgcc ttccggaagg aatgaccttc cagatacctc tccaatgagt agtattcaaa
     5341 cagcgtcacc accaagtcaa ttactgaccg atcaaggatt tgcggatttg gataatttca
     5401 tgtcttcgtt aggttctgtt tctaaaggtg aagaattatt cactggtgtt gtcccaattt
     5461 tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt gaaggtgaag
     5521 gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt aaattgccag
     5581 ttccatggcc aaccttagtc actactttat cttggggtgt tcaatgtttt gcaagatacc
     5641 cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt tatgttcaag
     5701 aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa gtcaagtttg
     5761 aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa gaagatggta
     5821 acattttagg tcacaaattg gaatacaatt atttctctga caatgtttac atcactgctg
     5881 acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt gaagatggtg
     5941 gtgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt ccagtcttgt
     6001 taccagacaa ccattactta tccactcaat ctaagttatc caaagatcca aacgaaaaga
     6061 gggaccacat ggtcttgtta gaatttgtta ctgctgctgg tattaccttg ggtatggatg
     6121 aattgtacaa aggatcctaa ctcgagagct tttgattaag ccttctagtc caaaaaacac
     6181 gtttttttgt catttatttc attttcttag aatagtttag tttattcatt ttatagtcac
     6241 gaatgtttta tgattctata tagggttgca aacaagcatt tttcatttta tgttaaaaca
     6301 atttcaggtt taccttttat tctgcttgtg gtgacgcgtg tatccgcccg ctcttttggt
     6361 cacccatgta tgctgccaaa ccagatgtca acacagctac aacggctagt ggcgaggggt
     6421 gacctaggat gtgaggcgag ggggactcta gataagcagg cgaggggctg aagcttgtca
     6481 atggcgaggg ggctagtggc gaggggcgac ctaggatgtg aggcgagggg gactctagat
     6541 aagcaggcga ggggctgaag cttgtcaatg gcgagggggc tatctccccg gaaactgtgg
     6601 ccttttctgg cacacatgat ctccacgatt tcaacatata aatagctttt gataatggca
     6661 atattaatca aatttatttt acttctttct tgtaacatct ctcttgtaat cccttattcc
     6721 ttctagctat ttttcataaa aaaccaagca actgcttatc aacacacaaa cacgctatat
     6781 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     6841 gcacatggag ggctccgtgg acggccacga gttcgagatc gagggcgagg gcgagggccg
     6901 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     6961 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     7021 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     7081 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     7141 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     7201 gcagaagaag actatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     7261 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     7321 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     7381 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     7441 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa tcctaactcg
     7501 aggccaattg gtgcggcaat tgataataac gaaaatgtct tttaatgatc tgggtataat
     7561 gaggaatttt ccgaacgttt ttactttata tatatatata catgtaacat atattctata
     7621 cgctatagag aaaggaaatt tttcaattaa aaaaaaaata gagaaagagt ttcacttctt
     7681 gattatcgct aacactaatg gttgaagtac tgctacttta attttatgct gagcagttac
     7741 agagatgtta cgaaccacta gtgcactgca gtacacggtt tccttgaaat ttttttgatt
     7801 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     7861 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     7921 gaacaaaaac gtgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     7981 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     8041 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     8101 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     8161 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     8221 aggacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     8281 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     8341 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     8401 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     8461 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     8521 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     8581 gagatgcatt gggtcaacag tatagaaccg tggatgatgt ggtttctaca ggatctgaca
     8641 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     8701 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     8761 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     8821 atcagttatt acccgagtta tcgtgttgca tctgcggctt taaattgatg tatctcatcg
     8881 caggcacggg cagtacagtg ccctgagcgt agggaaaaat gaaaaaaagg atgtaacttt
     8941 taacataatt ccagcacgca gcgattgggt ataatgaaga ttgttaagtt caacaacatg
     9001 tgaaattctt agctccgcaa cttgcttgaa ttatgagctc taagattcaa gaagtaaata
     9061 ggtcatataa tggaatgata gtagtacttg ataaaggaac catgtgatct tacgttgata
     9121 tgaaaataat tctctcacag aagtcagttt gtatgactaa catagagagt aattgctttt
     9181 cggataaaat cgcacctaac aaagattacc ttttgtaaag cctccagaac agctatgaat
     9241 gttcgttatt ctacgcactt tctttcatac atcttgatcc gccttttttg atgtaggttt
     9301 taacgcggca aaacatatta atttttctga tttggaaaaa taaagagtta gttctgcc
//
