LOCUS       Exported               10445 bp ds-DNA     circular SYN 06-APR-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      complement(13..39)
                     /label=KanR Terminator
                     /note="color: #93ff35; direction: LEFT"
     CDS             complement(40..855)
                     /label=KanR
                     /note="color: #008000; direction: LEFT"
     primer_bind     53..73
                     /label=MS17_KanF
                     /note="color: black; sequence: tcgagcatcaaatgaaactgc"
     promoter        complement(856..1003)
                     /label=KanR Promoter
                     /note="color: #93ff35; direction: LEFT"
     rep_origin      complement(1004..1767)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     primer_bind     1139..1158
                     /label=EC22_ColE1
                     /note="color: black; sequence: cagcggtggtttgtttgccg"
     primer_bind     1719..1740
                     /label=MM19
                     /note="color: black; sequence: tgctcacatgttctttcctgcg"
     primer_bind     1721..1740
                     /label=Je06_lvl0seq_F
                     /note="color: black; sequence: ctcacatgttctttcctgcg"
     protein_bind    1776..1779
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    1780..2279
                     /label=HO 5' Homology
                     /note="color: #6685ca"
     primer_bind     2123..2142
                     /label=IL53_Full_ampli_fw
                     /note="color: black; sequence: GCTCTACGAGCCTGTTGGAG"
     primer_bind     2230..2254
                     /label=IL80_pYAP_ampli_fw
                     /note="color: black; sequence: GCACATCGATTATTTGATACCCCTT"
     primer_bind     2230..2252
                     /label=IL48_HO5_full_fw
                     /note="color: black; sequence: GCACATCGATTATTTGATACCCC"
     misc_feature    complement(2280..2299)
                     /label=HO 5' barcode
                     /note="color: #8c3031; direction: LEFT"
     primer_bind     complement(2280..2299)
                     /label=MM_HO (2)
                     /note="color: black; sequence: ACAGCGATGGAACTTACGGC"
     protein_bind    2300..2303
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    2319..2343
                     /label=ConS scar
                     /note="color: #fffd6c"
     protein_bind    2319..2322
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     2340..2361
                     /label=MM29
                     /note="color: black; sequence: gacgaacgCCTTGCAATTAGCT"
     primer_bind     2344..2374
                     /label=Haa1-Promoter Primer Type2_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAaacgCCTTGCAATTAGCTTCTATTACACCTG"
     misc_feature    2344..2347
                     /label=Adaptor for Type2 entry vector
                     /note="color: #ff00ff"
     misc_feature    2348..3325
                     /label=HAA1 Native Promoter
                     /note="color: #b1ff67"
     primer_bind     2602..2619
                     /label=PrimerMM0_1-2
                     /note="color: black; sequence: CAACGTTGCCATCGTTGG"
     primer_bind     2797..2815
                     /label=MM30
                     /note="color: black; sequence: AATTGGTGGTGCCGGTTGT"
     primer_bind     3000..3022
                     /label=PrimerMM0_1-3
                     /note="color: black; sequence: GGTCTTAGTAATACAGAGTCGTG"
     primer_bind     3012..3031
                     /label=EC112_pHAA1_check
                     /note="color: black; sequence: ACAGAGTCGTGCATTTCCAC"
     primer_bind     3136..3155
                     /label=LT44_sgRNAcheck_HAA1
                     /note="color: black; sequence: GAGCCAAGACAAAACGTGGC"
     primer_bind     complement(3278..3329)
                     /label=Haa1 Promoter Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataTATTTTTTAGGTTTTTTTTTTCTATACTTTTGTTTCC
                     TTTTTCTTGTC"
     misc_feature    3326..3329
                     /label=Adaptor for Type 3a Entry Vector
                     /note="color: #ff00ff"
     misc_feature    3330..3902
                     /label=Bm3R1 DNA Binding Domain
                     /note="color: #99cc00"
     primer_bind     3398..3419
                     /label=MM31
                     /note="color: black; sequence: CGACGCTACCACTATGCCAATG"
     misc_feature    3903..3904
                     /label=linker
                     /note="Needed to avoid frame shift in the final construct 
                     for the next part of the fusion protein (Haa1-CFP)"
                     /note="color: #0000ff"
     primer_bind     3905..3938
                     /label=Haa1-CFP type 3b FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAttctATGGTCTTGATAAATGGCATAAAGTATGCC"
     misc_feature    3905..3908
                     /label=Adaptor for type 3b entry vector
                     /note="color: #ff00ff"
     misc_feature    3909..5990
                     /label=HAA1
                     /note="color: #b7e6d7"
     primer_bind     3909..3938
                     /label=Haa1 Primer Type3a_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAtatgATGGTCTTGATAAATGGCATAAAGTATGCC"
     primer_bind     3942..3961
                     /label=EC81_HAA1_F2
                     /note="color: black; sequence: GAGAGGTGCATAAGAGGCCA"
     primer_bind     complement(4020..4040)
                     /label=EC82_HAA1_R2
                     /note="color: black; sequence: GTCGCATGTAGTGGAAGGTCT"
     primer_bind     complement(4020..4040)
                     /label=EC113_HAA1_check
                     /note="color: black; sequence: GTCGCATGTAGTGGAAGGTCT"
     primer_bind     4185..4205
                     /label=primerMM0_2-2
                     /note="color: black; sequence: TGCGGGACTGATGAGGTTTGC"
     primer_bind     4342..4361
                     /label=EC79_HAA1_F
                     /note="color: black; sequence: GCCACGGAGCACTATCAGAT"
     primer_bind     complement(4397..4416)
                     /label=EC80_HAA1_R
                     /note="color: black; sequence: TTTTACCAACACCCGGCTCA"
     primer_bind     4623..4642
                     /label=MM32
                     /note="color: black; sequence: TCGGTAAGCTTGCCAGCGAA"
     primer_bind     complement(4635..4654)
                     /label=MS37
                     /note="color: black; sequence: CGTGAATCAGCTTTCGCTGG"
     primer_bind     4689..4708
                     /label=primerMM0_2-3
                     /note="color: black; sequence: CTTGACCTATTAGGCCATTC"
     primer_bind     4868..4887
                     /label=MM2s
                     /note="color: black; sequence: TACAACCGCAACCGCTGCAA"
     primer_bind     5184..5202
                     /label=primerMM0_2-4
                     /note="color: black; sequence: AATCCTCAACTTCGTCATC"
     primer_bind     5232..5253
                     /label=MM33
                     /note="color: black; sequence: CATCATCCGGCAAACGAGTATT"
     primer_bind     5499..5518
                     /label=MM3s
                     /note="color: black; sequence: GCCGTAGCAAGCAATCTCGA"
     primer_bind     complement(5654..5675)
                     /label=MM21_Rw
                     /note="color: black; sequence: CTTGTCTTGGCGACTTCCAGTT"
     primer_bind     5715..5733
                     /label=primerMM0_2-5
                     /note="color: black; sequence: AACATACACGATCTGTCAC"
     primer_bind     complement(5961..5996)
                     /label=Haa1 Primer Type3a_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAagaaCCTAACGAAGACATGAAATTATCCAAATCCGC"
     primer_bind     complement(5961..5993)
                     /label=Haa1/Haa1prom Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataccTAACGAAGACATGAAATTATCCAAATCCGC"
     misc_feature    5991..5992
                     /label=Stop Codon substitute
                     /note="color: #3366ff"
     protein_bind    5993..5996
                     /label=BsaI
                     /note="color: #00ff2c"
     CDS             5997..6710
                     /label=mTurquoise2
                     /note="color: #40e0d0; direction: RIGHT"
     primer_bind     6017..6038
                     /label=EC103_VENUS_F
                     /note="color: black; sequence: ATTCACTGGTGTTGTCCCAATT"
     primer_bind     complement(6081..6100)
                     /label=EC104_VENUS_R
                     /note="color: black; sequence: CCTTCACCTTCACCGGAGAC"
     primer_bind     complement(6516..6540)
                     /label=EC65_Venus_check
                     /note="color: black; sequence: aatggtcagctaattgaacaccacc"
     primer_bind     6516..6537
                     /label=EC105_VENUS_F2
                     /note="color: black; sequence: GGTGGTGTTCAATTAGCTGACC"
     primer_bind     complement(6685..6717)
                     /label=Haa1-CFP type 3b RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAggatcctttgtacaattcatccatacccaagg"
     protein_bind    6713..6716
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      6726..6950
                     /label=ScENO1 Terminator
                     /note="color: #ff8eff"
     primer_bind     complement(6739..6758)
                     /label=Je02tENO1SeqR
                     /note="color: black; sequence: tgttttttggactagaaggc"
     primer_bind     6897..6917
                     /label=MM23
                     /note="color: black; sequence: TATTCTGCTTGTGGTGACGCG"
     primer_bind     complement(6899..6918)
                     /label=EC195_Eno1_TT
                     /note="color: black; sequence: ACGCGTCACCACAAGCAGAA"
     primer_bind     6947..6968
                     /label=MM38
                     /note="color: black; sequence: gtatgctgccaaaccagatgtc"
     protein_bind    6951..6954
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    6955..6979
                     /label=Con1 scar
                     /note="color: #fffd6c"
     protein_bind    6955..6958
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     6980..7014
                     /label=YGP1 Primer Type2_FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAaacgGAGCAATAGGAAAATTCAGTAGTCGATTTCC"
     misc_feature    6980..6983
                     /label=Adaptor for Type2 entry vector
                     /note="color: #ff00ff"
     misc_feature    6984..7864
                     /label=YGP1-HREs Promoter
                     /label=YGP1 Native Promoter
                     /note="color: #b4abac"
     misc_feature    7017..7025
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7038..7046
                     /label=HRE2
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    complement(7047..7051)
                     /label=RGM1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    7111..7115
                     /label=RGM1 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    7128..7137
                     /label=MIG1 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    7136..7144
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    complement(7148..7157)
                     /label=MIG1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(7164..7170)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    7169..7177
                     /label=My change - 2
                     /note="color: #ff00ff; direction: RIGHT"
     misc_feature    complement(7176..7183)
                     /label=GCN4 BS
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     7210..7229
                     /label=MM34
                     /note="color: black; sequence: ACATCGGCCATTCGTTGGTT"
     misc_feature    complement(7233..7239)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     complement(7272..7285)
                     /label=MM22_Rw
                     /note="color: black; sequence: GATCTTCCGCGGATGACGAG"
     misc_feature    7286..7294
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7287..7294
                     /label=HRE2
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    7294..7298
                     /label=CHA4 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(7336..7342)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(7364..7370)
                     /label=CBF1 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    complement(7403..7410)
                     /label=HRE1
                     /note="color: #99cc00; direction: LEFT"
     misc_feature    7414..7423
                     /label=GAT3 BS
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    7427..7435
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    7431..7435
                     /label=RPH1 BS
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     7474..7496
                     /label=MM8s
                     /note="color: black; sequence: TTCCTAATGGAGAGGCTCAGGAG"
     misc_feature    7500..7508
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    7501..7508
                     /label=HRE1
                     /note="color: #99cc00; direction: RIGHT"
     misc_feature    7551..7559
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    complement(7616..7624)
                     /label=PHO2 BS
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    7637..7645
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7654..7662
                     /label=NEW HRE
                     /note="color: #ffff00; direction: RIGHT"
     misc_feature    7726..7734
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7778..7786
                     /label=NEW HRE
                     /note="color: #ffcc00; direction: RIGHT"
     misc_feature    7816..7823
                     /label=HCM1 BS
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(7827..7868)
                     /label=YGP1 Primer Type2_RW
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAcataTTTCTATTACTGTATTACTTAACTGACGATTAATCCT
                     G"
     primer_bind     7864..7883
                     /label=mCherry Type 3 FW
                     /note="color: black; sequence: 
                     GCATCGTCTCATCGGTCTCAtatggtgagcaagggcgag"
     primer_bind     7865..7885
                     /label=MM35
                     /note="color: black; sequence: tatggtgagcaagggcgagga"
     misc_feature    7865..7868
                     /label=Adaptor for Type 3
                     /note="color: #ff00ff"
     CDS             7866..8576
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8009..8029)
                     /label=LT82_mCherry_check_R
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGGCG"
     primer_bind     complement(8011..8029)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGG"
     primer_bind     8279..8298
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     primer_bind     8289..8310
                     /label=LT83_mCherry_check_F
                     /note="color: black; sequence: CAGAAGAAGACTATGGGCTGGG"
     primer_bind     8492..8513
                     /label=MM36
                     /note="color: black; sequence: caacgaggactacaccatcgtg"
     primer_bind     complement(8553..8581)
                     /label=mCherry Type 3 Rw
                     /note="color: black; sequence: 
                     ATGCCGTCTCAGGTCTCAggatttacttgtacagctcgtccatgcc"
     protein_bind    8577..8580
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      8590..8814
                     /label=ScSSA1 Terminator
                     /note="color: #ff8eff"
     protein_bind    8815..8818
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    8819..8843
                     /label=ConE scar
                     /note="color: #fffd6c"
     protein_bind    8819..8822
                     /label=BsmBI
                     /note="color: #00ff15"
     primer_bind     complement(8853..8874)
                     /label=MM24
                     /note="color: black; sequence: TCAAGGAAACCGTGTACTGCAG"
     protein_bind    8859..8862
                     /label=BsaI
                     /note="color: #00ff2c"
     promoter        8863..9039
                     /label=ScURA3 Promoter
                     /note="color: #ffb334"
     CDS             9040..9843
                     /label=URA3
                     /note="color: #ff9127; direction: RIGHT"
     primer_bind     9144..9166
                     /label=MM37
                     /note="color: black; sequence: attggatgttcgtaccaccaagg"
     terminator      9844..9921
                     /label=ScURA3 Terminator
                     /note="color: #ffcb72"
     protein_bind    9922..9925
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    9926..9945
                     /label=HO 3' barcode
                     /note="color: #0e7002"
     primer_bind     9926..9945
                     /label=MM_HO (3)
                     /note="color: black; sequence: TATCGTGTTGCATCTGCGGC"
     misc_feature    9946..10445
                     /label=HO 3' Homology
                     /note="color: #6685ca"
     primer_bind     complement(9978..9995)
                     /label=IL49_HO3_full_rev
                     /note="color: black; sequence: GCTCAGGGCACTGTACTG"
     primer_bind     complement(10030..10049)
                     /label=IL54_Full_ampli_rev
                     /note="color: black; sequence: GCTGCGTGCTGGAATTATGT"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgccaatt ctaattgtat cgagatcact
     1801 tttcgtgatc cgctaatcag cgacggtcac attaggtttg ccaagtcagg gtatgaacca
     1861 tacgatcagt tttcgtgaac ctggtacgta tattgtggcg tttgtgtata ttttcattct
     1921 ttgacaacaa tcaataccaa cctcaaatag gaaaagtaat aagtttggcg ttacacccca
     1981 aaagacgcca aacggatcga acttactcaa tagcaattag cgagacaaaa cctacgttaa
     2041 gacctgtaac cgatttatca aagcactctg cggttctttc ttgggaatat tacctggaca
     2101 ttttgtgccc tcaagaaacg aggctctacg agcctgttgg agcccctcag acattagccg
     2161 ccacgaatca aactttttac gcgattcggc ccaaatcagt ttctcacaga tcattcgtag
     2221 agtgaaaaag cacatcgatt atttgatacc cctttgggtt aattactgtt gaggtctttg
     2281 ccgtaagttc catcgctgtc cctgaattcg catctagact gaactggccg ataattgcag
     2341 acgaacgcct tgcaattagc ttctattaca cctgatgact gtttttcaat ctttatgtga
     2401 tctttttaca cccaatgtga taaagtacta gtaacatgaa tactactaaa cagatgatac
     2461 tttagagttt tcattccaac actcaattgt aaaaataaga caatattatg attttttggc
     2521 tgtataacca catctattta gtagcagggt gacatgttac tcccacacaa tacttcgcgc
     2581 aagtggttta gtggtaaaat ccaacgttgc catcgttggg cccccggttc gattccgggc
     2641 ttgcgcaatt tttccccctc gccctgaagg gcgtaacgtt aacgcttccg gtaatttgcg
     2701 aataattttt cttcccattt cccctttctt ttccctttaa catgtggagc atagcaggct
     2761 ctttactttt ttacgcattc agacagtcgg gaggtcaatt ggtggtgccg gttgtgggaa
     2821 agaggtaatt ttatttcctt tattggacat gatttcaata gtcacattgg ggcaggaaaa
     2881 agaaaaggga gaaaaaaatt cagtaaagaa aaaaaataag aaaaagagaa aagaaacaag
     2941 agagggctac agatttctaa atatcctttt gctgcacatt ttttatttac ctgctaattg
     3001 gtcttagtaa tacagagtcg tgcatttcca ctttataaga aaaaaaaaag ggataacagc
     3061 accagcactt gattgaaaac tagaaagttc acgttttata agtcctttcc gttctcttta
     3121 tcagttataa taaaagagcc aagacaaaac gtggcaggga agcttttcca aggaaaagag
     3181 ggttgtaata ttaaacaaaa accgtaatca agcaaaataa gaccccgtgg ttttttatca
     3241 taggaaaaga gttctctgtt gtcactctac catatttgac aagaaaaagg aaacaaaagt
     3301 atagaaaaaa aaaacctaaa aaatatatgg aatctactcc tactaagcaa aaagccatct
     3361 tctctgcctc cttgttgttg ttcgctgaga gaggtttcga cgctaccact atgccaatga
     3421 ttgccgaaaa cgctaaggtt ggtgctggta ctatctacag atacttcaag aacaaagaat
     3481 ccttagttaa tgagttgttt caacaacacg tcaatgaatt tttgcaatgt atcgaatctg
     3541 gtttggctaa cgaaagagat ggttacaggg acggtttcca ccacatcttc gaaggtatgg
     3601 tcaccttcac caagaaccat cctagagcct taggtttcat caagacccac tctcaaggta
     3661 cttttttgac cgaagaatcc agattggctt atcaaaagtt ggtcgaattt gtctgtactt
     3721 tcttcagaga aggtcaaaag caaggtgtca tcagaaattt gccagaaaac gctttgattg
     3781 ctatcttgtt cggttctttc atggaagtct acgaaatgat tgaaaacgat tatttgtctt
     3841 tgactgatga attattaacc ggtgttgagg aatccttgtg ggctgccttg tctcgtcaat
     3901 ctggttctat ggtcttgata aatggcataa agtatgcctg tgagaggtgc ataagaggcc
     3961 atagagtaac aacatgcaat catacagatc aaccgcttat gatgatcaaa cccaaaggta
     4021 gaccttccac tacatgcgac tattgtaaac aacttcgaaa aaacaagaat gcaaatcctg
     4081 aaggtgtttg cacgtgtggc cggctagaga agaaaaaact ggcacagaaa gccaaagaag
     4141 aagcaagagc taaagccaaa gaaaaacaaa gaaaacagtg tacctgcggg actgatgagg
     4201 tttgcaaata tcatgctcaa aagagacatc taagaaagtc cccttcaagt tctcaaaaga
     4261 aaggaagatc catttctcgt tctcaaccaa tgtttgaaag ggtattgtct tctacttcac
     4321 ttgacagcaa tatgttatcc ggccacggag cactatcaga tacctctagc atactgacga
     4381 gcacattttt agacagtgag ccgggtgttg gtaaaatttc aaaagattac catcatgtcc
     4441 cttcattggc ctccatttca tccttacaat cctcgcaatc gttagatcaa aatttcagta
     4501 taccacaaag cccgccgtta tcttcaatgt catttaattt tctcacggga aatatcaatg
     4561 aaaccaacca aaatcacagt aatcatcagc attcaaaatc aggcaataac tggcaagata
     4621 gttcggtaag cttgccagcg aaagctgatt cacgtcttaa catgatggat aaaaacaact
     4681 ctgtgggtct tgacctatta ggccattcaa aacgaatatc gccgatatca aactctcgtg
     4741 tgggcgaagt tagcgttccg ctagaagaat atattccttc tgacattgat ggggttggaa
     4801 gagttactga taaaagctct ttggtctacg attggccatt tgatgaaagt attgagagaa
     4861 atttcagtac aaccgcaacc gctgcaactg gtgaaagtaa gttcgacatt aacgacaact
     4921 gtaatagaat taatagcaaa agttatagta agactaatag tatgaatgga aacggtatga
     4981 acaatagcaa taataataat atcaacagta atggcaacga caagaacaat aacaactctt
     5041 ctagacaaga acatcaagga aatggactat ttgacatgtt tacagattca tcgtcgattt
     5101 caacgctttc ccgtgcaaac ttattattgc aagaaaaaat tggttcgcaa gaaaactctg
     5161 tcaaacaaga aaactattcg aaaaatcctc aacttcgtca tcaattaact tccagaagta
     5221 gatcatttat tcatcatccg gcaaacgagt atttgaagaa tacttttgga aattcacata
     5281 gtaatgacat cggaaaggga gttgaagtgc tatctttgac accgagtttt atggatattc
     5341 ccgaaaaaga aagagaaacg gaaagatcgc catcatccaa ttacattact gacagacctt
     5401 tcactcgaaa acctagatct tctagcattg acgtaaacca taggtatcca cctatggcac
     5461 caacaaccgt agcgacatct cccggtgcat tgaacaatgc cgtagcaagc aatctcgacg
     5521 atcaactgag tttaacatca ctaaactctc agccatcatc gatagcaaat atgatgatgg
     5581 acccttcaaa cctagctgag caaagttcta ttcattcagt tcctcagtca ataaactctc
     5641 cgagaatgcc taaaactgga agtcgccaag acaagaacat tcacactaag aaggaagaaa
     5701 gaaatccgct aaataacata cacgatctgt cacaattgga aaatgtacca gacgagatga
     5761 accaaatgtt ctccccacca ttaaaaagta tgaatagacc ggatgccata agggaaaatt
     5821 catctagtag taatttcata atccaaggaa atagcatgat ctctacgcct tccggaagga
     5881 atgaccttcc agatacctct ccaatgagta gtattcaaac agcgtcacca ccaagtcaat
     5941 tactgaccga tcaaggattt gcggatttgg ataatttcat gtcttcgtta ggttctgttt
     6001 ctaaaggtga agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg
     6061 ttaatggtca caaattttct gtctccggtg aaggtgaagg tgatgctact tacggtaaat
     6121 tgaccttaaa atttatttgt actactggta aattgccagt tccatggcca accttagtca
     6181 ctactttatc ttggggtgtt caatgttttg caagataccc agatcatatg aaacaacatg
     6241 actttttcaa gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag
     6301 atgacggtaa ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata
     6361 gaatcgaatt aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg
     6421 aatacaatta tttctctgac aatgtttaca tcactgctga caaacaaaag aatggtatca
     6481 aagctaactt caaaattaga cacaacattg aagatggtgg tgttcaatta gctgaccatt
     6541 atcaacaaaa tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat
     6601 ccactcaatc taagttatcc aaagatccaa acgaaaagag ggaccacatg gtcttgttag
     6661 aatttgttac tgctgctggt attaccttgg gtatggatga attgtacaaa ggatcctaac
     6721 tcgagagctt ttgattaagc cttctagtcc aaaaaacacg tttttttgtc atttatttca
     6781 ttttcttaga atagtttagt ttattcattt tatagtcacg aatgttttat gattctatat
     6841 agggttgcaa acaagcattt ttcattttat gttaaaacaa tttcaggttt accttttatt
     6901 ctgcttgtgg tgacgcgtgt atccgcccgc tcttttggtc acccatgtat gctgccaaac
     6961 cagatgtcaa cacagctaca acggagcaat aggaaaattc agtagtcgat ttccatggcg
     7021 aggggctatt gggtaatgtg gcggcgaggg ctaaacattc ggtatcattg attcaatgtt
     7081 ctcacaatgt tcaaattctc ggatcgggta tccctaattg ggatctcttt ccagaggcga
     7141 ggggcttgat gtgggattga agacacatga gtttttttct caccgtcgaa acttttgctt
     7201 acccgtaaaa catcggccat tcgttggttt attatgtgac ttttttgtga cttttttttt
     7261 tgcgaagttt tctcgtcatc cgcggggcga ggggcgggcg aaaaagtctc tcacttttac
     7321 ccaatcgggg catgttatgt ggacatgtta tgtgtccaag agacacatga gcccaaaaag
     7381 ggtacactgt ctaaatagtt taccctttac gaatcgaaac ttctcgggcg aggggccgaa
     7441 aattagcgca gaagttgacg aaagtttata cttttcctaa tggagaggct caggagctgg
     7501 gcgaggggat attcgtactc tattgcatct tcaaactccg aagaatctca ggcgaggggg
     7561 aagccggctt ctcgatgcta cagttcaaca tcgatacttt gaagaaagaa agcgcctatt
     7621 atatctcttt taccctggcg aggggaatta ggaggcgagg ggaaaaaagt acctcactaa
     7681 aaaaaccatc atctctgaaa tataaaaagc ttgatagagg gtgacggcga ggggaacttc
     7741 tgctgtgttc tcttgggtta ttgctcttat tgaatatggc gaggggttct ttcttgcttg
     7801 taaaaaatca gctcaaaaaa catctacagg attaatcgtc agttaagtaa tacagtaata
     7861 gaaatatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     7921 tcaaggtgca catggagggc tccgtggacg gccacgagtt cgagatcgag ggcgagggcg
     7981 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     8041 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     8101 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     8161 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     8221 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     8281 ccgtaatgca gaagaagact atgggctggg aggcctcctc cgagcggatg taccccgagg
     8341 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     8401 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     8461 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     8521 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaatcc
     8581 taactcgagg ccaattggtg cggcaattga taataacgaa aatgtctttt aatgatctgg
     8641 gtataatgag gaattttccg aacgttttta ctttatatat atatatacat gtaacatata
     8701 ttctatacgc tatagagaaa ggaaattttt caattaaaaa aaaaatagag aaagagtttc
     8761 acttcttgat tatcgctaac actaatggtt gaagtactgc tactttaatt ttatgctgag
     8821 cagttacaga gatgttacga accactagtg cactgcagta cacggtttcc ttgaaatttt
     8881 tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg
     8941 gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa
     9001 ctgcacagaa caaaaacgtg caggaaacga agataaatca tgtcgaaagc tacatataag
     9061 gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa
     9121 aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta
     9181 gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat
     9241 ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta
     9301 ctcttcgagg acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg
     9361 ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca
     9421 ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt
     9481 ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt
     9541 actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac
     9601 atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat
     9661 gacaagggag atgcattggg tcaacagtat agaaccgtgg atgatgtggt ttctacagga
     9721 tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag
     9781 ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac
     9841 taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt
     9901 taattatatc agttattacc cgagttatcg tgttgcatct gcggctttaa attgatgtat
     9961 ctcatcgcag gcacgggcag tacagtgccc tgagcgtagg gaaaaatgaa aaaaaggatg
    10021 taacttttaa cataattcca gcacgcagcg attgggtata atgaagattg ttaagttcaa
    10081 caacatgtga aattcttagc tccgcaactt gcttgaatta tgagctctaa gattcaagaa
    10141 gtaaataggt catataatgg aatgatagta gtacttgata aaggaaccat gtgatcttac
    10201 gttgatatga aaataattct ctcacagaag tcagtttgta tgactaacat agagagtaat
    10261 tgcttttcgg ataaaatcgc acctaacaaa gattaccttt tgtaaagcct ccagaacagc
    10321 tatgaatgtt cgttattcta cgcactttct ttcatacatc ttgatccgcc ttttttgatg
    10381 taggttttaa cgcggcaaaa catattaatt tttctgattt ggaaaaataa agagttagtt
    10441 ctgcc
//
