LOCUS       Exported                8717 bp ds-DNA     circular SYN 17-DEC-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLV-NRE-5xNFkB-mIL1Ra
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 20, 2022 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     Ligation of : pLV-5xNFkB-mIL1Ra.txt.xdna [8696 nt] : (EcoRI[3368] / 
            EcoRI[3368]) to [13 nt] : (Uncut 3'[13] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    239..815
                     /label=CMV_imm_early
     misc_feature    318..865
                     /label=CAG_enhancer
     LTR             835..1015
                     /label=HIV-1 5 LTR
     misc_feature    1126..1170
                     /label=HIV-1 psi pack
     misc_feature    1680..1913
                     /label=RRE
     misc_feature    2444..2459
                     /label=cPPT
     misc_feature    complement(2620..3156)
                     /label=mIL1Ra CDS
     misc_feature    complement(3169..3228)
                     /label=hCMVmp
     misc_feature    complement(3234..3264)
                     /label=insert from Tandem Repeat Sequence.xdna
                     /note="note=""Fragment extracted from Tandem Repeat 
                     Sequence.xdna [31 nt] : (XmaI[1] / BglII[36])"
     misc_feature    complement(3235..3244)
                     /label=Binding Site 5
     misc_feature    complement(3256..3266)
                     /label=Binding Site 4
     misc_feature    complement(3267..3280)
                     /label=Binding Site 3
                     complement(3269..3319)
                     /note="note=""Fragment extracted from PARALLEL - 3X flanked
                     by NheI and XmaI overhang.txt.xdna [51 nt] : (NheI[1] / 
                     XmaI[56])"
     misc_feature    complement(3286..3297)
                     /label=Binding Site 2
     misc_feature    complement(3308..3319)
                     /label=Binding Site 1
                     3373..3385
                     /note="note=""Fragment extracted from [13 nt] : (Uncut 
                     3'[13] / Uncut 5'[0])"
     misc_feature    3411..3998
                     /label=WPRE
     CDS             complement(3880..3891)
                     /label=FactorXa site
     misc_feature    4185..4206
                     /label=U3PTT
     misc_feature    4185..4200
                     /label=cPPT
     LTR             4522..4702
                     /label=HIV-1 5 LTR
     terminator      4731..4958
                     /label=bGH PA
     misc_feature    5626..5703
                     /label=SV40 ORI
     promoter        5822..5888
                     /label=EM7 promoter
     promoter        5822..5888
                     /label=mPGK Prom
     CDS             5889..6263
                     /label=ZeoR
     primer_bind     complement(6559..6579)
                     /label=M13-rev
     misc_binding    complement(6585..6607)
                     /label=LacO
     promoter        complement(6612..6641)
                     /label=lac
     rep_origin      complement(6947..7575)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7727..8386)
                     /label=AmpR
     promoter        complement(8626..8654)
                     /label=Amp prom
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta attacaggcc tcggcagtac tattggtctt cctggaagta
     2641 gaacttcgtg actataaggg gctcttccgg tgtgttggtg aggctcacag gacggtcagc
     2701 ctctagtgtt gtgcagagga accatcctgg acaggcagct gactcaaagc tggtggtggg
     2761 gcctttctca gagcggatga aggtaaagcg cttgtcttct tctttgttct tgctcagatc
     2821 agtgatgtta acttcctcca gctggagctt gatatcatct ccagacttgg cacaagacag
     2881 gcacagcttg cccccgtgga tgcccaagaa cacactatga aggtcaatag gcaccatgtc
     2941 tatcttttct tctagtttga tatttggtcc ttgtaagtac ccagcaatga gctggttgtt
     3001 tctcaggtaa aaggtcttct ggttagtatc ccagattctg aaggcttgca tcttgcaggg
     3061 tcttttccca gaagggcggc aggctgcctc tgaatgaaac agaaggatga gaaggagaga
     3121 gattaggtga ctgtagggtc cccagcagat ttccatggtg gcaagcttga tctgacggtt
     3181 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccag atctgggaag
     3241 tcccctcgag gagacgggaa agtcccgggc taatttccca ctagtgggaa agtcccacta
     3301 gtgctagcgg aatttcccgc tagcacgcgt aagagctcgg tacctctagc tagtcgagct
     3361 caacttcgaa tttctagatc agaggaattc gatatcaagc ttatcgataa tcaacctctg
     3421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3781 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3961 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     4021 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     4081 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     4141 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4201 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     4261 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     4321 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     4381 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     4441 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     4501 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4561 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4621 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4681 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4741 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4801 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4861 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4921 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4981 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5041 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5101 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5161 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5221 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5281 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5341 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5401 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5461 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5521 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5581 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5641 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5701 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5761 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5821 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     5881 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     5941 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     6001 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     6061 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     6121 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     6181 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     6241 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     6301 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6361 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6541 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctgac
//
