LOCUS       Exported                8533 bp ds-DNA     circular SYN 22-FEB-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Carolyn Huang
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 22, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..12
                     /label=3>6
                     /note="color: #a6acb3"
     misc_feature    13..33
                     /label=Pt4_common_region_6>4
                     /note="color: #a6acb3"
     misc_feature    15..18
                     /label=6>4 OH
                     /note="color: #0000ff"
     misc_feature    complement(19..22)
                     /label=SMRT Gate OH Right
                     /note="color: #ff6600; direction: LEFT"
     misc_feature    complement(27..33)
                     /label=AarI
                     /note="color: #cc99ff; direction: LEFT"
     rep_origin      complement(73..1538)
                     /direction=LEFT
                     /label=SC101
                     /note="color: #ff9ccd"
     CDS             complement(588..1538)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /note="color: #993366"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1586..1808
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
                     /note="color: #ffff00"
     misc_feature    2290..2293
                     /label=BsmBI_OH
                     /note="color: #cc99ff"
     misc_feature    2295..2324
                     /label=Part4_Ori_AbR_Common
                     /note="color: #a6acb3"
     terminator      complement(2325..2351)
                     /label=KanR Terminator
                     /note="color: #93ff35; direction: LEFT"
     CDS             complement(2352..3167)
                     /label=KanR
                     /note="color: #008000; direction: LEFT"
     promoter        complement(3168..3315)
                     /label=KanR Promoter
                     /note="color: #93ff35; direction: LEFT"
     misc_feature    3316..3340
                     /label=Pt4_common_region_4>5
                     /note="color: #a6acb3"
     misc_feature    3316..3322
                     /label=AarI
                     /note="color: #cc99ff; direction: RIGHT"
     misc_feature    3327..3330
                     /label=SMRT Gate OH Left
                     /note="color: #ff6600; direction: RIGHT"
     misc_feature    3331..3332
                     /label=spacer in case extra trimming during AarI GG
                     /note="color: #a6acb3"
     misc_feature    3333..3336
                     /label=pBBR1 Backbone Index (GACT)
                     /note="color: #33cccc; direction: BOTH"
     misc_feature    3337..3340
                     /label=4>5 OH
                     /label=4b(3)
                     /note="color: #0000ff"
     misc_feature    3341..3344
                     /label=1>2
                     /note="color: #a6acb3"
     CDS             complement(3352..4434)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    complement(3470..3475)
                     /label=old bsmbi
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(3601..3606)
                     /label=old bbsi
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(3940..3945)
                     /label=old bbsi
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(4435..4512)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    4705..4844
                     /label=pLac_approximately
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    4805..4821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     protein_bind    4828..4844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    4872..4877
                     /label=RBS
                     /note="color: #a6acb3"
     CDS             join(4885..8533,1..8)
                     /codon_start=1
                     /label=C2c3
                     /note="color: #ffef86"
                     /translation="MTKHSIPLHAFRNSGADARKWKGRIALLAKRGKETMRTLQFPLEM
                     SEPEAAAINTTPFAVAYNAIEGTGKGTLFDYWAKLHLAGFRFFPSGGAATIFRQQAVFE
                     DASWNAAFCQQSGKDWPWLVPSKLYERFTKAPREVAKKDGSKKSIEFTQENVANESHVS
                     LVGASITDKTPEDQKEFFLKMAGALAEKFDSWKSANEDRIVAMKVIDEFLKSEGLHLPS
                     LENIAVKCSVETKPDNATVAWHDAPMSGVQNLAIGVFATCASRIDNIYDLNGGKLSKLI
                     QESATTPNVTALSWLFGKGLEYFRTTDIDTIMQDFNIPASAKESIKPLVESAQAIPTMT
                     VLGKKNYAPFRPNFGGKIDSWIANYASRLMLLNDILEQIEPGFELPQALLDNETLMSGI
                     DMTGDELKELIEAVYAWVDAAKQGLATLLGRGGNVDDAVQTFEQFSAMMDTLNGTLNTI
                     SARYVRAVEMAGKDEARLEKLIECKFDIPKWCKSVPKLVGISGGLPKVEEEIKVMNAAF
                     MDVRARMFVRFEEIAAYVASKGAGMDVYDALEKRELEQLKKLKSAVPERAHIQAYRAVL
                     HRIGRAVQNCSEKTKQLFSSKVIEMGVFKNPSHLNNFIFNQKGAIYRSPFDRSRHAPYQ
                     LHADKLLKNDWMELLAEISTTLMASESTEQMEDALRLERTRLQLQLSGLPDWEYPASLA
                     KPDIEVEIQTALKMQLAKDTVTSDVLQRTFNLYSSVLSGLTFKLLRRSFSLKMRFSVAD
                     TTQLIYVPKDCDWAIPKQYLQAEGEIGIAARVVTESSPAKMVTEVEMKEPKALGHFMQQ
                     APHDWYFDASLGGTQVAGRIVEKGKEVGKERKLVGYRMRGNSAYKTVLDKSLVGNTELS
                     QCSMIIEIPYTQTVDADFRAQVQAGLPKVSINLPVKETITASNKDEQMLFDRFVAIDLG
                     ERGLGYAVFDAKTLELQESGHRPIKAITNLLNRTHHYEQRPNQRQKFQAKFNVNLSELR
                     ENTVGDVCHQINRICAYYNAFPVLEYMVPDRLDKQLKSVYESVTNRYIWSSTDAHKSAR
                     VQFWLGGETWEHPYLKSAKDKKPLVLSPGRGASGKGTSQTCSCCGRNPFDLIKDMKPRA
                     KIAVVDGKAKLENSELKLFERNRESKDDMLARRHRNERAGMEQPLTPGNYTVDEIKALL
                     RANLRRAPKNRRTKDTTVSEYHCVFSDCGKTMHADENAAVNIGGKFIADIEK"
     misc_feature    4886..4888
                     /label=2>3
                     /note="color: #a6acb3"
     misc_feature    4888
                     /label=part of start codon
                     /note="color: #a6acb3"
     misc_feature    7666..7668
                     /label=active site res
                     /note="color: #faac61"
ORIGIN
        1 agaagtgagc tgcaaggagg actttggcag gtgctctcct gagtaggaca aatccgccgc
       61 cctagaccta gggtacgggt tttgctgccc gcaaacgggc tgttctggtg ttgctagttt
      121 gttatcagaa tcgcagatcc ggcttcaggt ttgccggctg aaagcgctat ttcttccaga
      181 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg
      241 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa
      301 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc
      361 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt
      421 taaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat
      481 ctgtgcatat ggacagtttt ccctttgata tctaacggtg aacagttgtt ctacttttgt
      541 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
      601 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
      661 ccattgagat catgcttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
      721 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttacg taggtaggaa
      781 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
      841 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
      901 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
      961 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
     1021 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
     1081 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
     1141 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
     1201 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
     1261 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
     1321 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg
     1381 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
     1441 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
     1501 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
     1561 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
     1621 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
     1681 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
     1741 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
     1801 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
     1861 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
     1921 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
     1981 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
     2041 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
     2101 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
     2161 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
     2221 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
     2281 tactgtccct agtgtcagat ttcttacttt attcaggcga ttacgtgtta caaccaatta
     2341 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     2401 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     2461 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     2521 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2581 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     2641 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2701 attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa
     2761 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2821 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     2881 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2941 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     3001 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     3061 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     3121 tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta
     3181 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     3241 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     3301 gagttgaagg atcagcacct gcccgaacca gagacttatc tcaggttgcg ctcactgccc
     3361 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3421 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg atacgggcaa
     3481 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     3541 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     3601 gtcctcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc
     3661 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     3721 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     3781 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     3841 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     3901 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcctcatggg agaaaataat
     3961 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     4021 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     4081 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     4141 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     4201 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     4261 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     4321 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     4381 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     4441 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
     4501 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     4561 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     4621 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     4681 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     4741 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     4801 agaattgtga gcggataaca attgacattg tgagcggata acaagatact gagcaccgtg
     4861 ttaactttaa gaaggagata taccatgacc aagcacagca tccctctgca cgccttcaga
     4921 aactctggcg ccgatgccag aaagtggaag ggcagaattg ccctgctggc caagaggggc
     4981 aaagaaacca tgcggaccct gcagttccct ctggaaatga gcgaacctga ggccgctgcc
     5041 atcaacacca caccttttgc cgtggcctac aacgccatcg aaggcacagg caagggcacc
     5101 ctgtttgact actgggccaa actgcacctg gccggcttca gattctttcc atctggcgga
     5161 gccgccacca tctttaggca gcaggccgtg tttgaggacg cctcttggaa tgccgccttc
     5221 tgtcagcaga gcggcaagga ttggccttgg ctggtgccta gcaagctgta cgagcggttc
     5281 acaaaggccc ctagagaggt ggccaagaag gacggcagca agaagtccat cgagttcacc
     5341 caagagaacg tggccaacga gagccacgtg tcacttgtgg gcgccagcat caccgataag
     5401 acccctgagg accagaaaga atttttcctg aagatggcag gcgccctggc cgagaagttc
     5461 gattcttgga agtccgccaa cgaggaccgg atcgtggcca tgaaagtgat cgacgagttc
     5521 ctgaagtccg agggcctgca tctgcccagc ctggaaaaca ttgccgtgaa gtgcagcgtg
     5581 gaaacaaagc ccgacaatgc cacagtggcc tggcacgatg ctcctatgag cggagtgcag
     5641 aatctggcca tcggagtgtt tgccacctgt gccagccgga tcgacaacat ctacgacctg
     5701 aacggcggca agctgtccaa gctgattcaa gagagcgcca ccacaccaaa cgtgacagcc
     5761 ctgtcttggc tgttcggcaa aggcctggaa tacttccgga ccaccgacat cgacaccatc
     5821 atgcaggact tcaacatccc cgccagcgcc aaagagtcca tcaagcctct ggtggaaagc
     5881 gcccaggcta tccctaccat gacagtgctg ggcaagaaga actacgcccc tttcagaccc
     5941 aactttggcg gcaagatcga cagctggatc gccaactacg ccagcagact gatgctgctg
     6001 aacgacatcc tggaacagat cgagcccggc ttcgaactgc ctcaggctct gctggacaac
     6061 gagacactga tgagcggcat cgacatgacc ggcgacgagc tgaaagaact gatcgaggcc
     6121 gtgtacgcct gggttgacgc cgctaaacaa ggactggcca cactgcttgg cagaggcggc
     6181 aatgtggatg atgccgtgca gaccttcgag cagttcagcg ccatgatgga caccctgaac
     6241 ggaaccctga acaccatcag cgccagatat gtgcgcgccg tggaaatggc cggaaaggat
     6301 gaagcccggc tggaaaagct gatcgagtgc aagttcgaca tccccaagtg gtgcaagagc
     6361 gtgccaaagc tcgtgggcat ttctggcggc ctgcctaagg tggaagagga aatcaaagtg
     6421 atgaacgccg ccttcatgga cgtgcgggcc agaatgttcg tcagattcga agagatcgcc
     6481 gcctacgtgg cctctaaagg cgccggaatg gatgtgtacg acgccctgga aaagagagag
     6541 ctggaacagc tcaagaaact caagagcgcc gtgcctgaga gagcccacat ccaggcttat
     6601 agagccgtgc tgcacagaat cggacgggct gtgcagaact gcagcgaaaa gaccaagcag
     6661 ctgttctcca gcaaagtgat tgagatgggc gtgttcaaga accccagcca cctgaacaac
     6721 ttcatcttca accagaaggg cgccatctac agaagcccct tcgacagatc tcggcacgcc
     6781 ccatatcagc tgcacgccga taagctgctg aagaacgact ggatggaact gctggccgaa
     6841 atcagcacca cactgatggc cagcgagtcc accgagcaga tggaagatgc cctgagactg
     6901 gaacggacca gactgcaact gcagctgtct ggcctgcctg actgggagta tcctgcctct
     6961 ctggccaagc ctgacatcga ggtggaaatc cagaccgctc tgaagatgca gctggccaaa
     7021 gacaccgtga ccagcgacgt cctgcagcgg accttcaatc tgtacagctc tgtgctgagc
     7081 ggcctgacct tcaagctgct gcggagaagc ttcagcctga aaatgcggtt cagcgtggcc
     7141 gacaccacac agctgatcta cgtgcccaag gactgcgact gggctatccc caagcagtat
     7201 ctgcaggccg agggcgagat tgggatcgcc gctagagtgg tcacagagtc tagccccgcc
     7261 aagatggtca ccgaagtgga aatgaaggaa cccaaggctc tgggccactt catgcagcag
     7321 gctccccacg actggtactt cgatgcctct ctcggaggaa ctcaggtggc cggcagaatc
     7381 gtggaaaagg gaaaagaagt cggcaaagag cggaagctcg tcggctacag aatgagaggc
     7441 aacagcgcct acaagaccgt gctggataag agcctcgtgg gaaacaccga gctgagccag
     7501 tgctctatga tcatcgagat cccttacaca cagaccgtgg acgccgactt cagagcacag
     7561 gttcaagccg gcctgccaaa agtgtctatc aacctgcctg tgaaagagac aatcaccgcc
     7621 agcaacaagg atgagcagat gctgttcgat cgctttgtgg ccatcgacct gggcgagaga
     7681 ggactgggat acgccgtgtt cgatgccaag acactggaac tgcaagagtc cggccaccgg
     7741 cctatcaagg ccatcaccaa cctgctgaac cggacccatc actacgagca gaggcccaac
     7801 cagagacaga agttccaggc caagttcaac gtgaacctga gcgagctgcg cgagaatact
     7861 gtgggcgacg tgtgccacca gatcaacaga atctgcgcct actacaacgc tttccccgtg
     7921 ctcgagtaca tggtgcccga cagactggac aaacagctga agtctgtgta cgagagcgtg
     7981 accaaccggt acatctggtc cagcacagac gcccacaaaa gcgctcgcgt gcagttttgg
     8041 ctcggcggag aaacatggga gcacccctac ctgaaaagcg ccaaggacaa gaaacccctg
     8101 gtgctgtctc ctggaagagg cgcttctggc aagggaacca gccagacctg tagctgctgt
     8161 ggcagaaacc ctttcgacct gatcaaggac atgaagccca gggccaagat cgccgtggtg
     8221 gatggaaaag ccaagctgga aaattccgag ctgaagctgt ttgagcggaa ccgcgagagc
     8281 aaggacgaca tgctggctcg gagacacaga aacgagcgcg ctggaatgga acagcctctg
     8341 acacccggca actacaccgt ggatgagatc aaggccctgc tgagagccaa cctgagaagg
     8401 gcccctaaga acagacggac caaggacacc accgtgtccg agtaccactg cgtgttcagc
     8461 gattgcggca agaccatgca tgccgacgaa aacgccgccg tgaacatcgg cggaaagttt
     8521 atcgccgata tcg
//
